IN2014DN10053A - - Google Patents

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Publication number
IN2014DN10053A
IN2014DN10053A IN10053DEN2014A IN2014DN10053A IN 2014DN10053 A IN2014DN10053 A IN 2014DN10053A IN 10053DEN2014 A IN10053DEN2014 A IN 10053DEN2014A IN 2014DN10053 A IN2014DN10053 A IN 2014DN10053A
Authority
IN
India
Prior art keywords
component
nkx6
endodermal cell
noggin
egf
Prior art date
Application number
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English (en)
Inventor
Gordon Keller
Maria Cristina Nostro
Audrey Holtzinger
Farida Sarangi
Original Assignee
Univ Health Network
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Health Network filed Critical Univ Health Network
Publication of IN2014DN10053A publication Critical patent/IN2014DN10053A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
IN10053DEN2014 2012-04-30 2013-04-30 IN2014DN10053A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261640366P 2012-04-30 2012-04-30
PCT/CA2013/000432 WO2013163739A1 (en) 2012-04-30 2013-04-30 METHODS AND COMPOSITIONS FOR GENERATING PANCREATIC PROGENITORS AND FUNCTIONAL BETA CELLS FROM hPSCs

Publications (1)

Publication Number Publication Date
IN2014DN10053A true IN2014DN10053A (ja) 2015-08-14

Family

ID=49514141

Family Applications (1)

Application Number Title Priority Date Filing Date
IN10053DEN2014 IN2014DN10053A (ja) 2012-04-30 2013-04-30

Country Status (18)

Country Link
US (1) US20150104430A1 (ja)
EP (2) EP3594323A1 (ja)
JP (2) JP6397397B2 (ja)
CN (2) CN108004199B (ja)
AU (1) AU2013255031C1 (ja)
CA (1) CA2871795C (ja)
CY (1) CY1122058T1 (ja)
DK (1) DK2844739T3 (ja)
ES (1) ES2747967T3 (ja)
HK (2) HK1208047A1 (ja)
HR (1) HRP20191677T1 (ja)
HU (1) HUE046271T2 (ja)
IN (1) IN2014DN10053A (ja)
LT (1) LT2844739T (ja)
PL (1) PL2844739T3 (ja)
PT (1) PT2844739T (ja)
SI (1) SI2844739T1 (ja)
WO (1) WO2013163739A1 (ja)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014165663A1 (en) * 2013-04-03 2014-10-09 Cellular Dynamics International, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
WO2015178431A1 (ja) * 2014-05-21 2015-11-26 国立大学法人京都大学 膵芽細胞の製造方法および膵芽細胞を含む膵疾患治療剤
JP6954711B2 (ja) 2015-04-24 2021-10-27 ユニバーシティ オブ コペンハーゲン 真正膵臓前駆細胞の単離
WO2016170067A1 (en) * 2015-04-24 2016-10-27 University Of Copenhagen Method for production of insulin-producing cells
WO2017019702A1 (en) * 2015-07-27 2017-02-02 The Regents Of The University Of California Methods and compositions for producing pancreatic beta cells
CN106554936B (zh) * 2015-09-30 2020-05-05 海门雨霖细胞科技有限责任公司 诱导人干细胞向肝细胞定向分化的新方法
US11274280B2 (en) 2016-02-24 2022-03-15 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
MA45329A (fr) 2016-04-08 2019-02-13 Univ California Production de cellules bêta matures pleinement fonctionnelles à partir de progénitrices pancréatiques humaines
WO2018021293A1 (ja) * 2016-07-26 2018-02-01 国立大学法人京都大学 Ｐｔｆ１ａ陽性細胞の製造方法
US20190390169A1 (en) * 2017-03-03 2019-12-26 Kyoto University Pancreatic progenitor cell production method
CN111246864A (zh) * 2017-07-21 2020-06-05 森玛治疗公司 干细胞衍生的胰腺β细胞的重聚
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
IL305391A (en) 2017-11-15 2023-10-01 Vertex Pharma Preparations for the production of islet cells and methods of use
CN111630052B (zh) 2018-01-18 2023-04-18 第一三共株式会社 二氢吲嗪酮衍生物
EP3801577A4 (en) 2018-05-31 2021-07-14 University Health Network (UHN) METHOD AND COMPOSITIONS USING TANKYRASE INHIBITOR FOR GENERATING INSULIN-PRODUCING CELLS
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
AU2018439389A1 (en) 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
CN109053866B (zh) * 2018-09-03 2020-12-15 北京中京高科生物科技有限公司 表皮干细胞向胰腺细胞分化的改进方法
EP3868870A4 (en) * 2018-10-15 2022-10-19 Evia Life Sciences Inc. METHOD OF GENERATING STEM/PROGENIOR CELLS USING SMALL MOLECULAR COMPOUNDS FROM CELLS DERIVED FROM AN ENDODERMAL TISSUE OR ORGAN
JP7385244B2 (ja) * 2019-06-27 2023-11-22 国立大学法人 東京大学 膵前駆細胞の分離方法
WO2021051256A1 (en) * 2019-09-17 2021-03-25 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Pancreatic pro-endocrine progenitor cells and use thereof
CN114634904B (zh) * 2022-05-17 2022-09-13 天津外泌体科技有限公司 高纯度胰腺祖细胞的产生方法

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US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
US5945577A (en) 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
KR101089591B1 (ko) * 2001-12-07 2011-12-05 제론 코포레이션 인간 배아 줄기세포 유래의 섬세포
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Also Published As

Publication number Publication date
AU2013255031A1 (en) 2014-12-04
CA2871795C (en) 2023-03-14
AU2013255031B2 (en) 2018-09-06
CN108004199A (zh) 2018-05-08
PL2844739T3 (pl) 2019-12-31
WO2013163739A1 (en) 2013-11-07
HRP20191677T1 (hr) 2019-12-13
EP2844739B1 (en) 2019-06-19
US20150104430A1 (en) 2015-04-16
JP6759293B2 (ja) 2020-09-23
PT2844739T (pt) 2019-10-01
EP3594323A1 (en) 2020-01-15
HUE046271T2 (hu) 2020-02-28
JP6397397B2 (ja) 2018-09-26
CN108004199B (zh) 2022-03-18
CN104411815A (zh) 2015-03-11
JP2015519048A (ja) 2015-07-09
CY1122058T1 (el) 2020-11-25
LT2844739T (lt) 2019-10-10
CN104411815B (zh) 2017-11-28
SI2844739T1 (sl) 2019-11-29
HK1254968A1 (zh) 2019-08-02
CA2871795A1 (en) 2013-11-07
EP2844739A1 (en) 2015-03-11
EP2844739A4 (en) 2015-12-02
JP2018198620A (ja) 2018-12-20
AU2013255031C1 (en) 2018-12-06
ES2747967T3 (es) 2020-03-12
HK1208047A1 (en) 2016-02-19
DK2844739T3 (da) 2019-09-30

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