IN187721B - - Google Patents

Info

Publication number
IN187721B
IN187721B IN1479MA1998A IN187721B IN 187721 B IN187721 B IN 187721B IN 1479MA1998 A IN1479MA1998 A IN 1479MA1998A IN 187721 B IN187721 B IN 187721B
Authority
IN
India
Prior art keywords
insulin
pro
recombinant human
isolated
vector
Prior art date
Application number
Other languages
English (en)
Inventor
Astolf Filho Spartaco
De Lima Beatriz Dolabela
Josef Ernst Thiemann
De Heloisa Ribeiro Tunes Sousa
Luciano Vilela
Original Assignee
Univ Brasilia
Biobras S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brasilia, Biobras S A filed Critical Univ Brasilia
Publication of IN187721B publication Critical patent/IN187721B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IN1479MA1998 1997-07-02 1998-07-02 IN187721B (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/886,967 US6068993A (en) 1997-07-02 1997-07-02 Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin

Publications (1)

Publication Number Publication Date
IN187721B true IN187721B (fr) 2002-06-15

Family

ID=25390172

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1479MA1998 IN187721B (fr) 1997-07-02 1998-07-02

Country Status (11)

Country Link
US (4) US6068993A (fr)
EP (1) EP0996713B1 (fr)
AT (1) ATE278776T1 (fr)
BR (1) BR9810650B1 (fr)
CA (2) CA2294760C (fr)
DE (1) DE69826864T2 (fr)
IL (2) IL133832A0 (fr)
IN (1) IN187721B (fr)
PL (1) PL194141B1 (fr)
RU (1) RU2222598C2 (fr)
WO (1) WO1999001548A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7049286B2 (en) * 2001-08-30 2006-05-23 Diatos, S.A. Insulin conjugates and methods of use thereof
CA2504129A1 (fr) * 2002-11-01 2004-05-21 Promega Corporation Compositions de lyse cellulaire, leurs procedes d'utilisation, appareil et trousse associes
MXPA06003357A (es) 2003-09-26 2006-06-08 Japan Tobacco Inc Metodo para inhibir la produccion de lipoproteina remanente.
US7674621B2 (en) * 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
CA2580313C (fr) 2004-07-19 2016-03-15 Biocon Limited Conjugues insuline-oligomere, preparations et utilisations de ceux-ci
WO2006037215A1 (fr) * 2004-10-04 2006-04-13 National Research Council Of Canada Systeme d'expression, composants de ce systeme d'expression, et procedes d'utilisation
US7935788B2 (en) * 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
US8986997B2 (en) * 2007-08-31 2015-03-24 The Regents Of The University Of Michigan Methods and compositions for increasing biological molecule stability
BRPI0818004B8 (pt) 2007-10-16 2021-05-25 Biocon Ltd composição farmacêutica sólida administrável por via oral e o processo da mesma.
RU2570625C2 (ru) * 2010-03-31 2015-12-10 Амсилк Гмбх Выделение нерастворимых искомых белков
RU2497118C1 (ru) * 2012-03-06 2013-10-27 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) БЫСТРЫЙ СПОСОБ ОБРАБОТКИ ГЛАДКОМЫШЕЧНЫХ КЛЕТОК КИШЕЧНИКА ГЕМОЛИЗИНОМ II Bacillus cereus
CN102617703A (zh) * 2012-04-14 2012-08-01 拜明(苏州)生物技术有限公司 一种无需裂解高效提取发酵细菌中外源表达蛋白的方法
WO2015176067A2 (fr) 2014-05-16 2015-11-19 The Regents Of The University Of California Long arn non-codant exprimé dans un cancer aggressif
EP3344651B1 (fr) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. Procédé d'obtention d'insuline avec liaisons disulfure correctement formées

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704362A (en) * 1977-11-08 1987-11-03 Genentech, Inc. Recombinant cloning vehicle microbial polypeptide expression
US4624926A (en) * 1981-01-02 1986-11-25 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
NZ199391A (en) * 1981-01-02 1985-12-13 Genentech Inc Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
US4503155A (en) * 1982-02-01 1985-03-05 Eli Lilly And Company Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli
US4732859A (en) * 1982-09-03 1988-03-22 Eli Lilly And Company Method for conferring bacteriophage resistance to bacteria
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4578355A (en) * 1983-01-12 1986-03-25 The United States Of America As Represented By The Department Of Health And Human Services Plasmid cloning vector pAS1
US5256546A (en) * 1983-07-15 1993-10-26 Bio-Technology General Corp. Bacterial expression of porcine growth hormone
US4734368A (en) * 1984-03-23 1988-03-29 Huels Aktiengesellschaft Process for the bioconversion of fumarate to L-malate
US4870013A (en) * 1984-04-19 1989-09-26 Cetus Corporation SV40 early and RSV promoters useful in saccharomyces expression
US4581170A (en) * 1984-08-03 1986-04-08 E. R. Squibb & Sons, Inc. N-hydroxyl protecting groups and process and intermediates for the preparation of 3-acylamino-1-hydroxy-2-azetidinones
US5032510A (en) * 1984-09-26 1991-07-16 Eli Lilly And Company Method for expression and secretion in bacillus
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
CA1304886C (fr) * 1986-07-10 1992-07-07 Heinz Dobeli Resines chelatant des metaux
CA1339955C (fr) * 1986-10-14 1998-07-14 Richard Eugene Heiney Methode pour transformer un pecurseur d'insuline humaine en insuline humaine
SU1703693A1 (ru) * 1987-02-09 1992-01-07 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Рекомбинантна плазмидна ДНК @ - @ 2-19, кодирующа синтез интерлейкина-2 человека, способ ее конструировани @ штамм бактерий ЕSснеRIснIа coLI - продуцент интерлейкина-2 человека
CA1340522C (fr) * 1987-03-10 1999-05-04 Heinz Dobeli Proteins hybrides renfermant des histidines voisines pour une purification amelioree
US5063158A (en) * 1987-11-09 1991-11-05 Eli Lilly And Company Recombinant DNA expression vector comprising both transcriptional and translational activating sequences
CA1340772C (fr) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression et secretion de proteines heterologues dans une levure au moyen de sequences leader tronquees du facteur alpha
US5026639A (en) * 1988-01-14 1991-06-25 Nippon Mining Company, Limited Method to improve mRNA translation and use thereof for production of platelet factor-4
US5258287A (en) * 1988-03-22 1993-11-02 Genentech, Inc. DNA encoding and methods of production of insulin-like growth factor binding protein BP53
EP0337243A1 (fr) * 1988-04-14 1989-10-18 F. Hoffmann-La Roche Ag Procédé pour purifier de l'interleukine-2 recombinante
US5262322A (en) * 1988-06-24 1993-11-16 Genentech, Inc. Host transformed with yeast gene and ubiquitin/polypeptide fusions
US4897465A (en) * 1988-10-12 1990-01-30 Abbott Laboratories Enrichment and concentration of proteins by ultrafiltration
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
DE69101486T2 (de) * 1990-01-24 1994-08-04 Upjohn Co Verfahren zur reinigung rekombinanter polypeptide.
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US5460954A (en) * 1992-04-01 1995-10-24 Cheil Foods & Chemicals, Inc. Production of human proinsulin using a novel vector system
US5648235A (en) * 1992-07-03 1997-07-15 Q.B.I. Enterprises Ltd. Method and means for the production of gene products, novel recombinant DNA vectors therefor and kits employing them
GB9224584D0 (en) * 1992-11-23 1993-01-13 Connaught Lab Use of outer membrane protein d15 and its peptides as vaccine against haempohilus influenzae diseases
US5648244A (en) * 1993-09-27 1997-07-15 President And Fellows Of Harvard College Production, purification, cleavage and use of fusion peptides
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
US5534428A (en) * 1994-09-06 1996-07-09 Life Technologies, Inc. Cloned Ssti/SacI restriction-modification system
US5850015A (en) * 1995-06-07 1998-12-15 Cornell Research Foundation, Inc. Hypersensitive response elicitor from Erwinia chrysanthemi
ZA979496B (en) * 1996-10-31 1999-04-23 Genentech Inc Assay using marked microbial host cell strains

Also Published As

Publication number Publication date
EP0996713A1 (fr) 2000-05-03
BR9810650A (pt) 2002-07-23
EP0996713B1 (fr) 2004-10-06
CA2687542C (fr) 2013-04-30
DE69826864T2 (de) 2005-11-03
US6281329B1 (en) 2001-08-28
DE69826864D1 (de) 2004-11-11
WO1999001548A2 (fr) 1999-01-14
BR9810650B1 (pt) 2010-12-14
CA2294760A1 (fr) 1999-01-14
CA2294760C (fr) 2010-02-23
WO1999001548A3 (fr) 1999-09-10
RU2222598C2 (ru) 2004-01-27
US6068993A (en) 2000-05-30
US20030082742A1 (en) 2003-05-01
IL133832A (en) 2007-07-04
IL133832A0 (en) 2001-04-30
US6699692B2 (en) 2004-03-02
PL348669A1 (en) 2002-06-03
CA2687542A1 (fr) 1999-01-14
US6509452B1 (en) 2003-01-21
ATE278776T1 (de) 2004-10-15
PL194141B1 (pl) 2007-04-30

Similar Documents

Publication Publication Date Title
IN187721B (fr)
Laban et al. Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.
AU1672388A (en) Expression of human proapolipoprotein a-i
HK1004474A1 (en) Purified recombinant interleukin-2-compositions
WO2001046384A3 (fr) Technique d'isolation et de purification de cellules neuronales multipotentes progenitrices et cellules neuronales multipotentes progenitrices
NZ296748A (en) Peripheral nervous system specific sodium channel proteins, DNA coding therefor, compositions and methods
EP1559776A3 (fr) Systeme d'expression du Fusarium non pathogene, non toxicogene, non toxique et promoteurs et terminateurs utilisés dans ce systeme
WO1999020766A3 (fr) SEQUENCES D'ACIDES NUCLEIQUES MODIFIES ET PROCEDES PERMETTANT D'ACCROITRE LES NIVEAUX D'ARNm ET L'EXPRESSION DES PROTEINES DANS LES SYSTEMES CELLULAIRES
AU1276295A (en) Expression of self-processing polyproteins in transgenic plants
KR970701199A (ko) 성장 정지 호메오박스 유전자(Growth Arrest Homeobox Gene)
DK0587742T3 (da) Vitaminafhængig carboxylase
KR960701096A (ko) 그룹 B 스트렙토코커스의 많은 균주들에 대한 면역성을 제공하는 세포 표면 단백질인 Rib 단백질, 그 단백질의 정제방법, 시약키트 및 제약조성물(PROTEIN RIB. A CELL SURFACE PROTEIN THAT CONFERS IMMUNITY TO MANY STRAINS OF THE GROUP B STREPTOCOCCUS; PROCESS FOR PURIFICATION OF THE PROTEIN, PEAGENT KIT AND PHARMACEUTICAL COMPOSITION)
DE69735264D1 (de) Gefrierschutzproteine aus choristoneura sp., gene und verfahren zu ihrer verwendung
ATE286069T1 (de) Verfahren zur herstellung rekombinanter pilc- proteine
SE9501472D0 (sv) Truncated IGF-I
WO1998027200A3 (fr) Sequences consensus de l'origine de replication d'adn humain et d'adn de mammifere
AU5076393A (en) Proteins which regulate the expression of vertebrate MHC class II genes, DNA sequences encoding them and pharmaceutical compositions
Ito Effects of a protease inhibitor on biosynthesis of Escherichia coli proteins
WO2009054754A1 (fr) Plasmide recombinant phins21 codant une protéine hybride avec la proinsuline humaine, souche de bactéries escherichia coli jm109/ phins21 productrice de la protéine hybride avec la proinsuline humaine et procédé de fabrication de proinsuline humaine
ZA200007619B (en) New peptide fragments for protein purification.
AU2001253258A1 (en) Human trp-like calcium channel protein-2 (tlcc-2)
EP2270029B1 (fr) Système de sécrétion d'E. coli à base de protéines à couche S
ZA200203383B (en) Mimetic peptides for epitope of apolipoprotein B-100, concatemer and modified peptides thereof, and the vaccine composition comprising the same.
PT742829E (pt) Expressao de proteinas heterologas em bacterias atenuadas utilizando os promotores htra
Sokolik Cytochrome c as a model substrate for the ubiquitin system