IL89701A - Recombinant retroviruses method for the production thereof and pharmaceutical compositions containing the same - Google Patents
Recombinant retroviruses method for the production thereof and pharmaceutical compositions containing the sameInfo
- Publication number
- IL89701A IL89701A IL8970189A IL8970189A IL89701A IL 89701 A IL89701 A IL 89701A IL 8970189 A IL8970189 A IL 8970189A IL 8970189 A IL8970189 A IL 8970189A IL 89701 A IL89701 A IL 89701A
- Authority
- IL
- Israel
- Prior art keywords
- cells
- expression
- recombinant retrovirus
- retrovirus
- vector
- Prior art date
Links
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Applications Claiming Priority (1)
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| US17051588A | 1988-03-21 | 1988-03-21 |
Publications (2)
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| IL89701A0 IL89701A0 (en) | 1989-09-28 |
| IL89701A true IL89701A (en) | 1995-07-31 |
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| IL8970189A IL89701A (en) | 1988-03-21 | 1989-03-21 | Recombinant retroviruses method for the production thereof and pharmaceutical compositions containing the same |
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| Country | Link |
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| EP (2) | EP0702084B1 (de) |
| JP (5) | JPH03504079A (de) |
| CN (1) | CN1038306A (de) |
| AT (2) | ATE174960T1 (de) |
| AU (1) | AU651311B2 (de) |
| CA (1) | CA1341585C (de) |
| CH (1) | CH684094A5 (de) |
| DE (2) | DE68929122T2 (de) |
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| ES (1) | ES2139810T3 (de) |
| FI (1) | FI113476B (de) |
| GB (2) | GB2236753B (de) |
| GR (1) | GR3032940T3 (de) |
| IL (1) | IL89701A (de) |
| NO (1) | NO314596B1 (de) |
| WO (1) | WO1989009271A1 (de) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6241982B1 (en) | 1988-03-21 | 2001-06-05 | Chiron Corporation | Method for treating brain cancer with a conditionally lethal gene |
| US5662896A (en) | 1988-03-21 | 1997-09-02 | Chiron Viagene, Inc. | Compositions and methods for cancer immunotherapy |
| US5716826A (en) * | 1988-03-21 | 1998-02-10 | Chiron Viagene, Inc. | Recombinant retroviruses |
| US6569679B1 (en) | 1988-03-21 | 2003-05-27 | Chiron Corporation | Producer cell that generates adenoviral vectors encoding a cytokine and a conditionally lethal gene |
| US5591624A (en) * | 1988-03-21 | 1997-01-07 | Chiron Viagene, Inc. | Retroviral packaging cell lines |
| US6133029A (en) * | 1988-03-21 | 2000-10-17 | Chiron Corporation | Replication defective viral vectors for infecting human cells |
| US5614404A (en) * | 1988-06-10 | 1997-03-25 | Theriod Biologics, Incorporated | Self-assembled, defective, non-self-propagating lentivirus particles |
| US5747324A (en) * | 1988-06-10 | 1998-05-05 | Therion Biologics Corporation | Self-assembled, defective, non-self-propagating lentivirus particles |
| US5650148A (en) * | 1988-12-15 | 1997-07-22 | The Regents Of The University Of California | Method of grafting genetically modified cells to treat defects, disease or damage of the central nervous system |
| WO1990007936A1 (en) * | 1989-01-23 | 1990-07-26 | Chiron Corporation | Recombinant therapies for infection and hyperproliferative disorders |
| DE69033975T2 (de) * | 1989-01-23 | 2002-10-02 | Chiron Corp. (N.D.Ges.D. Staates Delaware), Emeryville | Rekombinanttherapien für Infektionen und hyperproliferative Störungen |
| JP3140757B2 (ja) * | 1989-02-06 | 2001-03-05 | デイナ・フアーバー・キヤンサー・インステイテユート | パッケージング欠陥ｈｉｖプロウイルス、細胞系及びその使用 |
| US5693622A (en) * | 1989-03-21 | 1997-12-02 | Vical Incorporated | Expression of exogenous polynucleotide sequences cardiac muscle of a mammal |
| US6673776B1 (en) | 1989-03-21 | 2004-01-06 | Vical Incorporated | Expression of exogenous polynucleotide sequences in a vertebrate, mammal, fish, bird or human |
| US5703055A (en) | 1989-03-21 | 1997-12-30 | Wisconsin Alumni Research Foundation | Generation of antibodies through lipid mediated DNA delivery |
| WO1990012087A1 (en) * | 1989-04-05 | 1990-10-18 | Novacell Corporation | Infectious targetted replication-defective virion |
| EP0487587A1 (de) * | 1989-08-18 | 1992-06-03 | Chiron Corporation | Rekombinante retroviren um vektorkonstruktionen an zielzellen zu liefern |
| US6337209B1 (en) | 1992-02-26 | 2002-01-08 | Glaxo Wellcome Inc. | Molecular constructs containing a carcinoembryonic antigen regulatory sequence |
| GB8919607D0 (en) * | 1989-08-30 | 1989-10-11 | Wellcome Found | Novel entities for cancer therapy |
| EP0897987B1 (de) * | 1989-10-24 | 2001-02-28 | Chiron Corporation | Sekretion vom mit Gamma-Interferon Signalpeptid gebundenen humänen Protein |
| FR2655855B1 (fr) * | 1989-12-20 | 1992-04-10 | Pasteur Institut | Protection genetique d'animaux contre l'infection par le virus leucemogene bovin, et provirus et virus recombinant derive de ce virus. |
| US6706694B1 (en) | 1990-03-21 | 2004-03-16 | Vical Incorporated | Expression of exogenous polynucleotide sequences in a vertebrate |
| US6228844B1 (en) | 1991-11-12 | 2001-05-08 | Vical Incorporated | Stimulating vascular growth by administration of DNA sequences encoding VEGF |
| US6555108B1 (en) * | 1990-09-14 | 2003-04-29 | The General Hospital Corporation | Implanting HSV-TK retrovirus producing cells to treat tumors |
| US6537541B1 (en) * | 1990-09-14 | 2003-03-25 | The General Hospital Corporation | Implantation of HSV-TK retrovirus producer cells to destroy glioma |
| WO1992005266A2 (en) * | 1990-09-21 | 1992-04-02 | Viagene, Inc. | Packaging cells |
| KR100372934B1 (ko) | 1990-09-25 | 2003-12-24 | 캔탑 파마슈티칼스 리서취 리미티드 | 형질 상보성 세포주에 의해 생성된 바이러스 결손 백신 |
| US5665362A (en) * | 1990-09-25 | 1997-09-09 | Cantab Pharmaceuticals Research Limited | Viral vaccines |
| US7374768B1 (en) | 1990-09-25 | 2008-05-20 | Xenova Research Limited | Viral vaccines |
| US5837261A (en) * | 1990-09-25 | 1998-11-17 | Cantab Pharmaceuticals Research Limited | Viral vaccines |
| GB9100612D0 (en) * | 1991-01-11 | 1991-02-27 | Univ Edinburgh | Method of altering the metabolic state of non-dividing cells,transgenic animals and therapeutic agents |
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| JPH06505401A (ja) * | 1991-10-30 | 1994-06-23 | ユニベルシテ・ピエール・エ・マリ・キュリー（パリ６） | ウイルス、特に病原性レトロウイルスによって誘発される疾病の予防または治療のための形質転換された細胞 |
| WO1993010218A1 (en) * | 1991-11-14 | 1993-05-27 | The United States Government As Represented By The Secretary Of The Department Of Health And Human Services | Vectors including foreign genes and negative selective markers |
| US7223411B1 (en) | 1992-07-31 | 2007-05-29 | Dana-Farber Cancer Institute | Herpesvirus replication defective mutants |
| GB2269175A (en) * | 1992-07-31 | 1994-02-02 | Imperial College | Retroviral vectors |
| EP0746625B1 (de) * | 1992-11-09 | 2004-03-31 | THE UNITED STATES GOVERNMENT as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES | Erzielbare vektorenpartikel |
| WO1994016072A1 (en) * | 1993-01-14 | 1994-07-21 | Intracell Therapeutics, Inc. | Vectors useful for controlling hiv-1 replication and methods for using the same |
| AU1257495A (en) | 1993-11-18 | 1995-06-06 | Chiron Corporation | Compositions and methods for utilizing conditionally lethal genes |
| US6451571B1 (en) | 1994-05-02 | 2002-09-17 | University Of Washington | Thymidine kinase mutants |
| EP0758387B1 (de) * | 1994-05-02 | 2001-12-19 | University of Washington | Thymidin-kinase-mutanten |
| US5888814A (en) * | 1994-06-06 | 1999-03-30 | Chiron Corporation | Recombinant host cells encoding TNF proteins |
| EP0763123A4 (de) * | 1994-06-07 | 1998-08-12 | Immunomedics Inc | Sichere retrovirale vektoren, ihre herstellung und verwendung |
| GB9414652D0 (en) * | 1994-07-20 | 1994-09-07 | Moeller Erna | Method of treatment |
| WO1997015679A1 (en) * | 1995-10-27 | 1997-05-01 | The Trustees Of The University Of Pennsylvania | Recombinant viruses containing mobile genetic elements and methods of use in gene therapy |
| FR2741358B1 (fr) * | 1995-11-17 | 1998-01-02 | Centre Nat Rech Scient | Production de vecteurs retroviraux par l'intermediaire de vecteurs viraux a base de virus a adn |
| US6153380A (en) | 1996-01-23 | 2000-11-28 | Rigel Pharmaceuticals, Inc. | Methods for screening for transdominant intracellular effector peptides and RNA molecules |
| US6365344B1 (en) | 1996-01-23 | 2002-04-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for screening for transdominant effector peptides and RNA molecules |
| US6776986B1 (en) | 1996-06-06 | 2004-08-17 | Novartis Ag | Inhibition of HIV-1 replication by antisense RNA expression |
| DE69737597D1 (de) * | 1996-07-22 | 2007-05-24 | Us Gov Health & Human Serv | Vektoren zur inhibierung von viralem und tumorwachstum |
| US6953687B1 (en) | 1997-07-17 | 2005-10-11 | The United States Of America As Represented By The Department Of Health And Human Services | Vectors for delivering viral and oncogenic inhibitors |
| US6544523B1 (en) | 1996-11-13 | 2003-04-08 | Chiron Corporation | Mutant forms of Fas ligand and uses thereof |
| GB9703802D0 (en) * | 1997-02-24 | 1997-04-16 | Kingsman Susan M | Anti-hiv peptides and proteins |
| EP2008664B1 (de) | 1997-04-10 | 2012-08-29 | University Of Southern California | Modifizierte Proteine zur Bindung extrazellulärer Matrixkomponenten |
| GB9711578D0 (en) * | 1997-06-04 | 1997-07-30 | Oxford Biomedica Ltd | Novel retroviral vector production systems |
| DE19737442C2 (de) * | 1997-08-22 | 1999-06-24 | Olfert Landt Tib Molbiol Synth | Proteinumhüllte Polyribonukleinsäuren, ein Verfahren zu ihrer Herstellung und ihre Verwendung |
| JP2001514235A (ja) * | 1997-09-02 | 2001-09-11 | カイロン コーポレイション | 遺伝子治療を利用して関節炎を処置するための組成物および方法 |
| EP1900818A3 (de) | 1997-11-06 | 2008-06-11 | Novartis Vaccines and Diagnostics S.r.l. | Neissersche Antigene |
| US6914131B1 (en) | 1998-10-09 | 2005-07-05 | Chiron S.R.L. | Neisserial antigens |
| WO1999036544A2 (en) | 1998-01-14 | 1999-07-22 | Chiron S.P.A. | Neisseria meningitidis antigens |
| US7078483B2 (en) | 1998-04-29 | 2006-07-18 | University Of Southern California | Retroviral vectors including modified envelope escort proteins |
| EP2261347A3 (de) | 1998-05-01 | 2012-01-11 | Novartis Vaccines and Diagnostics, Inc. | Antigene und Zusammensetzungen gegen Neisseria meningitidis |
| US6492166B1 (en) | 1998-07-29 | 2002-12-10 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Use of constitutive transport elements for host range control |
| US6399354B1 (en) | 1998-07-31 | 2002-06-04 | President And Fellows Of Harvard College | Replication-competent virus expressing a detectable fusion protein |
| US7935805B1 (en) | 1998-12-31 | 2011-05-03 | Novartis Vaccines & Diagnostics, Inc | Polynucleotides encoding antigenic HIV Type C polypeptides, polypeptides and uses thereof |
| EP1980617A1 (de) | 1998-12-31 | 2008-10-15 | Novartis Vaccines and Diagnostics, Inc. | Verbesserte Expression von HIV-Polypeptiden und Herstellung virusähnlicher Partikel |
| CA2372235A1 (en) | 1999-04-30 | 2000-11-09 | Rino Rappuoli | Conserved neisserial antigens |
| GB9911683D0 (en) | 1999-05-19 | 1999-07-21 | Chiron Spa | Antigenic peptides |
| GB9916529D0 (en) | 1999-07-14 | 1999-09-15 | Chiron Spa | Antigenic peptides |
| EP2169073B1 (de) * | 1999-10-11 | 2013-11-13 | Institut Pasteur | Vektoren für die Herstellung von immunotherapeutischen Zusammensetzungen |
| EP1226253A2 (de) | 1999-10-29 | 2002-07-31 | Chiron S.P.A. | Antigene peptide aus neisseria |
| CA2871789C (en) | 2000-01-17 | 2017-04-04 | Novartis Vaccines And Diagnostics S.R.L. | Outer membrane vesicle (omv) vaccine comprising n. meningitidis serogroup b outer membrane proteins |
| DK1947187T5 (da) | 2000-02-28 | 2011-10-24 | Novartis Vaccines & Diagnostic | Hybrid ekspression af neisserial-proteiner |
| ATE439372T1 (de) | 2000-10-27 | 2009-08-15 | Novartis Vaccines & Diagnostic | Nukleinsäuren und proteine von gruppen a und b- streptokokken |
| WO2002042468A2 (en) | 2000-11-27 | 2002-05-30 | Geron Corporation | Glycosyltransferase vectors for treating cancer |
| WO2002044394A2 (en) | 2000-11-29 | 2002-06-06 | University Of Southern California | Targetet retoviral vectors for cancer immunotherapy |
| FR2820146B1 (fr) * | 2001-01-29 | 2003-06-27 | Centre Nat Rech Scient | Procede d'obtention de cellules d'encapsidation fortement productrices de retrovirus par elimination, au sein de la culture, des cellules permissives a l'infection par le retro virus produit |
| GB0107661D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Staphylococcus aureus |
| GB0107658D0 (en) | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
| GB0111442D0 (en) * | 2001-05-10 | 2001-07-04 | Isis Innovation | Method for preparing recombinant virus |
| CA2452015C (en) | 2001-07-05 | 2012-07-03 | Chiron Corporation | Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof |
| US20030198621A1 (en) | 2001-07-05 | 2003-10-23 | Megede Jan Zur | Polynucleotides encoding antigenic HIV type B and/or type C polypeptides, polypeptides and uses thereof |
| EP2168596B1 (de) | 2001-12-12 | 2012-05-23 | Novartis Vaccines and Diagnostics S.r.l. | Immunisierung gegen Chlamydia trachomatis |
| GB0308198D0 (en) | 2003-04-09 | 2003-05-14 | Chiron Srl | ADP-ribosylating bacterial toxin |
| ES2366617T3 (es) | 2003-04-21 | 2011-10-21 | Epeius Biotechnologies Corporation | Métodos y composiciones para el tratamiento de transtornos. |
| US20070178066A1 (en) | 2003-04-21 | 2007-08-02 | Hall Frederick L | Pathotropic targeted gene delivery system for cancer and other disorders |
| US8268324B2 (en) | 2004-03-29 | 2012-09-18 | Galpharma Co., Ltd. | Modified galectin 9 proteins and use thereof |
| GB0606190D0 (en) | 2006-03-28 | 2006-05-10 | Isis Innovation | Construct |
| ATE522541T1 (de) | 2006-06-09 | 2011-09-15 | Novartis Ag | Bakterielle adhäsine konformere |
| CN107022217A (zh) * | 2017-06-08 | 2017-08-08 | 南通市乐佳涂料有限公司 | 一种采用水包水的环保抗锈涂料 |
| CN109722465B (zh) * | 2019-01-07 | 2022-02-01 | 福建省疾病预防控制中心(福建省健康教育促进中心、福建省卫生检验检测中心) | 一种hiv耐药检测载体和构建方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2559159B1 (fr) * | 1984-02-02 | 1986-09-12 | Inst Nat Sante Rech Med | Vecteurs viraux de clonage et d'expression d'une proteine dans une cellule eucaryote, comportant au moins une partie du genome d'un retro-virus; cellules eucaryotes transfectees; procede utilisant de telles cellules et proteines obtenues |
| CA1310924C (en) * | 1986-04-24 | 1992-12-01 | Francis P. Mccormick | Infective drug delivery system |
| FR2606030B1 (fr) * | 1986-10-31 | 1989-10-20 | Pasteur Institut | Retrovirus recombinant defectif, son procede de fabrication et ses applications, notamment pour l'etude de l'interaction des retrovirus sauvages avec des lignees cellulaires infectables par celui-ci |
| WO1990001870A1 (en) * | 1988-08-22 | 1990-03-08 | The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce | Retroviral vectors expressing soluble cd4; a gene therapy for aids |
-
1989
- 1989-03-20 CH CH4290/89A patent/CH684094A5/de not_active IP Right Cessation
- 1989-03-20 JP JP1503903A patent/JPH03504079A/ja not_active Withdrawn
- 1989-03-20 AU AU33640/89A patent/AU651311B2/en not_active Expired
- 1989-03-20 CN CN89102697A patent/CN1038306A/zh active Pending
- 1989-03-20 WO PCT/US1989/001139 patent/WO1989009271A1/en not_active Ceased
- 1989-03-21 EP EP95115441A patent/EP0702084B1/de not_active Revoked
- 1989-03-21 AT AT89105059T patent/ATE174960T1/de not_active IP Right Cessation
- 1989-03-21 DE DE68929122T patent/DE68929122T2/de not_active Revoked
- 1989-03-21 EP EP89105059A patent/EP0334301B1/de not_active Expired - Lifetime
- 1989-03-21 AT AT95115441T patent/ATE187983T1/de not_active IP Right Cessation
- 1989-03-21 CA CA005943744A patent/CA1341585C/en not_active Expired - Fee Related
- 1989-03-21 DE DE68928884T patent/DE68928884T2/de not_active Expired - Fee Related
- 1989-03-21 IL IL8970189A patent/IL89701A/en unknown
- 1989-03-21 ES ES95115441T patent/ES2139810T3/es not_active Expired - Lifetime
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1990
- 1990-09-20 FI FI904647A patent/FI113476B/fi not_active IP Right Cessation
- 1990-09-20 GB GB9020586A patent/GB2236753B/en not_active Expired - Lifetime
- 1990-09-20 NO NO19904103A patent/NO314596B1/no not_active IP Right Cessation
- 1990-09-20 DK DK199002268A patent/DK175857B1/da not_active IP Right Cessation
-
1992
- 1992-08-10 GB GB9217086A patent/GB2255777B/en not_active Expired - Fee Related
-
1998
- 1998-12-24 JP JP10368260A patent/JPH11262397A/ja not_active Withdrawn
-
2000
- 2000-03-10 GR GR20000400636T patent/GR3032940T3/el not_active IP Right Cessation
-
2002
- 2002-03-11 JP JP2002066181A patent/JP2002306188A/ja not_active Withdrawn
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2003
- 2003-10-10 JP JP2003353011A patent/JP2004008227A/ja active Pending
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2007
- 2007-08-01 JP JP2007200751A patent/JP2007289206A/ja active Pending
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