IL80073A - Protein preparations or recombinant viruses capable of inducing an immune response against lav/htlv III - Google Patents
Protein preparations or recombinant viruses capable of inducing an immune response against lav/htlv IIIInfo
- Publication number
- IL80073A IL80073A IL8007386A IL8007386A IL80073A IL 80073 A IL80073 A IL 80073A IL 8007386 A IL8007386 A IL 8007386A IL 8007386 A IL8007386 A IL 8007386A IL 80073 A IL80073 A IL 80073A
- Authority
- IL
- Israel
- Prior art keywords
- lav
- htlv iii
- virus
- recombinant
- vaccinia
- Prior art date
Links
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
- C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
- C12N2740/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
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- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
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- Gastroenterology & Hepatology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL10907894A IL109078A0 (en) | 1985-09-25 | 1994-03-22 | Vaccines and immunoassays for acquired immune deficiency syndrome using a recombinant virus that expresses an envelope or gag hiv protein or protein or peptide products thereof |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US77990985A | 1985-09-25 | 1985-09-25 | |
US84298486A | 1986-03-27 | 1986-03-27 | |
US90521786A | 1986-09-09 | 1986-09-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
IL80073A0 IL80073A0 (en) | 1986-12-31 |
IL80073A true IL80073A (en) | 1995-01-24 |
Family
ID=27419757
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL8007386A IL80073A (en) | 1985-09-25 | 1986-12-31 | Protein preparations or recombinant viruses capable of inducing an immune response against lav/htlv III |
Country Status (22)
Country | Link |
---|---|
CN (1) | CN1020752C (pl) |
AT (1) | ATA256786A (pl) |
CH (1) | CH676247A5 (pl) |
DE (1) | DE3690508T1 (pl) |
DK (1) | DK455486A (pl) |
ES (2) | ES2002490A6 (pl) |
FI (1) | FI863848A (pl) |
FR (1) | FR2587720A2 (pl) |
GB (1) | GB2181435B (pl) |
GR (1) | GR862412B (pl) |
HU (1) | HU205780B (pl) |
IE (1) | IE59314B1 (pl) |
IL (1) | IL80073A (pl) |
IT (1) | IT1195829B (pl) |
MY (1) | MY103182A (pl) |
NL (1) | NL8602422A (pl) |
NO (1) | NO863803L (pl) |
NZ (1) | NZ217645A (pl) |
PT (1) | PT83434B (pl) |
SE (4) | SE8604007L (pl) |
WO (1) | WO1987002038A1 (pl) |
YU (1) | YU46753B (pl) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3650011T2 (de) * | 1985-04-08 | 1994-11-17 | Genetic Systems Corp | EXPRESSION UND DIAGNOSE MIT gag-KODIERTEN PEPTIDEN, DIE MIT ANTIKÖRPERN GEGEN LAV IMMUNOLOGISCH REAKTIV SIND. |
US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
US4925784A (en) * | 1986-04-04 | 1990-05-15 | Hoffmann-La Roche Inc. | Expression and purification of an HTLV-III gag/env gene protein |
US4983387A (en) * | 1986-05-19 | 1991-01-08 | Viral Technologies Inc. | HIV related peptides, immunogenic antigens, and use therefor as subunit vaccine for AIDS virus |
ATE107861T1 (de) * | 1986-09-19 | 1994-07-15 | Oncogen | Verwendung von aktivierten t-lymphozyten zur vorbereitung einer pharmazeutischen zusammensetzung zur behandlung von aids. |
IE872748L (en) * | 1986-10-16 | 1988-04-16 | Arjomari Europ | Polypeptides derived from the evvelope gene of human¹immunodeficiency virus in recombinant baculovirus infected¹insect cells |
JPS63258575A (ja) * | 1986-12-15 | 1988-10-26 | レプリゲン コーポレーション | 昆虫細胞中でつくられる組換えhivエンベロープタンパク |
WO1988005440A1 (fr) * | 1987-01-16 | 1988-07-28 | Institut Pasteur | Peptides ayant des proprietes immunologiques 2-hiv-2 |
FR2610632B1 (fr) * | 1987-02-11 | 1990-12-21 | Pasteur Institut | Peptides caracteristiques des retrovirus d'immunodeficience humaine (virus hiv) leurs applications au diagnostic des infections dues a certains de ces virus et, le cas echeant, a la vaccination contre le sida |
US5681713A (en) * | 1987-05-08 | 1997-10-28 | Smithkline Beecham Corporation | Expression of heterologous proteins in Drosophila cells |
GB8714802D0 (en) * | 1987-06-24 | 1987-07-29 | Proteus Biotech Ltd | Synthetic polypeptides |
DE10399031I1 (de) * | 1987-08-28 | 2004-01-29 | Health Research Inc | Rekombinante Viren. |
FR2620030B1 (fr) * | 1987-09-07 | 1990-03-23 | Transgene Sa | Vecteur d'expression des proteines du virus hiv-2, un agent causal du sida, culture cellulaire infectee ou transformee par ce vecteur, proteines obtenues, vaccin et anticorps obtenus |
IL89118A0 (en) * | 1988-02-03 | 1989-08-15 | Microgenesys Inc | Vaccine containing polypeptides derived from the envelope gene of human immunodeficiency virus type 1 |
US5763160A (en) * | 1988-02-12 | 1998-06-09 | United Biomedical, Inc. | Synthetic peptides and process of using same for the detection of antibodies to human immunodeficiency virus (HIV) gp120 envelope protein, diagnosis of AIDS and pre-AIDS conditions and as vaccines |
ATE110112T1 (de) * | 1988-05-06 | 1994-09-15 | Ferropas Ag | Verfahren und systeme zur herstellung von hiv- antigenen. |
US5043262A (en) * | 1988-05-12 | 1991-08-27 | Dana Farber Cancer Institute | Protein, sequences containing the VPU gene therefore, vectors, methods of preparation and use |
AP129A (en) * | 1988-06-03 | 1991-04-17 | Smithkline Biologicals S A | Expression of retrovirus gag protein eukaryotic cells |
US5631154A (en) * | 1988-06-10 | 1997-05-20 | Therion Biologics, Incorporated | Self assembled, defective, non-self-propagating lentivirus particles |
WO1989012095A1 (en) * | 1988-06-10 | 1989-12-14 | Applied Biotechnology, Inc. | A method of evaluating recombinant vaccines against immunodeficiency virus |
US5747324A (en) * | 1988-06-10 | 1998-05-05 | Therion Biologics Corporation | Self-assembled, defective, non-self-propagating lentivirus particles |
US5614404A (en) * | 1988-06-10 | 1997-03-25 | Theriod Biologics, Incorporated | Self-assembled, defective, non-self-propagating lentivirus particles |
US5665577A (en) * | 1989-02-06 | 1997-09-09 | Dana-Farber Cancer Institute | Vectors containing HIV packaging sequences, packaging defective HIV vectors, and uses thereof |
WO1990012880A1 (en) * | 1989-04-18 | 1990-11-01 | Applied Biotechnology, Inc. | Generation of hybrid genes and proteins by virus-mediated recombination |
WO1990015141A2 (en) * | 1989-06-01 | 1990-12-13 | Applied Biotechnology, Inc. | Self-assembled, defective, non-self-propagating viral particles |
GB8923123D0 (en) * | 1989-10-13 | 1989-11-29 | Connaught Lab | A vaccine for human immunodeficiency virus |
US5981276A (en) * | 1990-06-20 | 1999-11-09 | Dana-Farber Cancer Institute | Vectors containing HIV packaging sequences, packaging defective HIV vectors, and uses thereof |
US5840312A (en) * | 1991-05-02 | 1998-11-24 | Institut Pasteur | Recombinant Bacillus anthracis strains unable to produce the lethal factor protein or edema factor protein |
FR2676068B1 (fr) * | 1991-05-02 | 1994-11-04 | Pasteur Institut | Souches recombinantes immunogenes de b. anthracis - compositions immunogenes les contenant. |
EP0611397B1 (en) * | 1991-11-08 | 2002-06-19 | PHARMACIA & UPJOHN COMPANY | Feline leukemia virus vaccines |
DE4405810A1 (de) | 1994-02-23 | 1995-08-24 | Behringwerke Ag | Von einem Retrovirus aus der HIV-Gruppe abgeleitete Peptide und deren Verwendung |
AU5259796A (en) | 1995-02-10 | 1996-08-27 | Worcester Foundation For Biomedical Research, Inc. | Delivery of exogenous compounds |
US6013506A (en) * | 1995-06-05 | 2000-01-11 | Wardley; Richard C. | Feline leukemia virus vaccines |
DE59507617D1 (de) * | 1995-09-06 | 2000-02-17 | Schablonentechnik Kufstein Ag | Verfahren zum Herstellen einer Siebdruckschablone |
US5741492A (en) * | 1996-01-23 | 1998-04-21 | St. Jude Children's Research Hospital | Preparation and use of viral vectors for mixed envelope protein vaccines against human immunodeficiency viruses |
US6723558B1 (en) | 1996-01-23 | 2004-04-20 | St. Jude Children's Research Hospital | Preparation and use of viral vectors for mixed envelope protein vaccines against human immunodeficiency viruses |
US20060165606A1 (en) | 1997-09-29 | 2006-07-27 | Nektar Therapeutics | Pulmonary delivery particles comprising water insoluble or crystalline active agents |
US7871598B1 (en) | 2000-05-10 | 2011-01-18 | Novartis Ag | Stable metal ion-lipid powdered pharmaceutical compositions for drug delivery and methods of use |
MXPA04005865A (es) | 2001-12-19 | 2004-09-13 | Nektar Therapeutics | Suministro de aminoglucosidos a los pulmones. |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3485810T2 (de) * | 1983-05-27 | 1992-12-10 | Texas A & M University Syst | Verfahren zur herstellung eines rekombinanten baculovirus-expressionsvektors. |
US4662514A (en) * | 1983-11-01 | 1987-05-05 | Charleswater Products, Inc. | Electrically conductive polymeric tubes for static sensitive electronic devices |
US9328391B1 (en) * | 1984-08-22 | 2016-05-03 | The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Cloning and expression of HIV-1 DNA |
IL76082A (en) * | 1984-08-22 | 1991-07-18 | Us Health | Molecular clones of the genome of htlv-iii and a process for the preparation thereof |
DE3588254T2 (de) * | 1984-10-18 | 2008-06-26 | Institut Pasteur | GAG-Antigen und dessen Verwendung zum Nachweis von LAV-Infektion, sowie in immunogenen Zusammensetzungen |
GB8501473D0 (en) * | 1985-01-21 | 1985-02-20 | Pasteur Institut | Cloned dna sequences |
CA1341423C (en) * | 1984-10-31 | 2003-03-04 | Paul A. Luciw | Recombinant proteins of viruses associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome |
EP0187041B1 (en) * | 1984-12-24 | 1996-05-15 | Genentech, Inc. | Fusions of AIDS-related polypeptides |
US4774175A (en) * | 1985-03-01 | 1988-09-27 | Centocor, Inc. | Immunochemical methods for the detection of antibody against HTLV-III |
DK171119B1 (da) * | 1985-04-19 | 1996-06-17 | Hoffmann La Roche | AIDS-viruskappeprotein, ekspressionsvektor bærende viruskappeproteinet, transformanter transformeret med ekspressionsvektoren, fremgangsmåde til fremstilling af viruskappeproteinet, fremgangsmåde til detektion af AIDS-antistoffer, fremgangsmåde til bestemmelse af AIDS-virus, vaccine mod AIDS, antistoffer mod viruskappeproteinet samt anvendelse af dette til fremstilling af en vaccine og til testnin |
FR2586427B1 (fr) * | 1985-08-20 | 1988-12-09 | Pasteur Institut | Sequences de nucleotides et de polypeptides issus du virus visna et leur application a des essais de diagnostic et a la production de compositions immunogenes |
-
1986
- 1986-09-22 GR GR862412A patent/GR862412B/el unknown
- 1986-09-22 NZ NZ217645A patent/NZ217645A/xx unknown
- 1986-09-23 IE IE252586A patent/IE59314B1/en not_active IP Right Cessation
- 1986-09-23 SE SE8604007A patent/SE8604007L/xx not_active Application Discontinuation
- 1986-09-24 ES ES8602123A patent/ES2002490A6/es not_active Expired
- 1986-09-24 DK DK455486A patent/DK455486A/da not_active Application Discontinuation
- 1986-09-24 NO NO863803A patent/NO863803L/no unknown
- 1986-09-24 IT IT67730/86A patent/IT1195829B/it active
- 1986-09-24 FI FI863848A patent/FI863848A/fi not_active IP Right Cessation
- 1986-09-24 HU HU864072A patent/HU205780B/hu not_active IP Right Cessation
- 1986-09-24 GB GB8622987A patent/GB2181435B/en not_active Expired - Fee Related
- 1986-09-24 CH CH3821/86A patent/CH676247A5/fr not_active IP Right Cessation
- 1986-09-25 AT AT0256786A patent/ATA256786A/de unknown
- 1986-09-25 NL NL8602422A patent/NL8602422A/nl not_active Application Discontinuation
- 1986-09-25 CN CN86106632A patent/CN1020752C/zh not_active Expired - Fee Related
- 1986-09-25 FR FR8613414A patent/FR2587720A2/fr active Granted
- 1986-09-25 DE DE19863690508 patent/DE3690508T1/de not_active Withdrawn
- 1986-09-25 YU YU165486A patent/YU46753B/sh unknown
- 1986-09-25 WO PCT/US1986/002002 patent/WO1987002038A1/en active Application Filing
- 1986-09-25 PT PT83434A patent/PT83434B/pt not_active IP Right Cessation
- 1986-12-31 IL IL8007386A patent/IL80073A/en not_active IP Right Cessation
-
1987
- 1987-09-29 MY MYPI87002188A patent/MY103182A/en unknown
-
1988
- 1988-05-13 ES ES8801494A patent/ES2006941A6/es not_active Expired
-
1991
- 1991-10-14 SE SE9102976A patent/SE9102976L/xx not_active Application Discontinuation
- 1991-10-14 SE SE9102974A patent/SE9102974L/xx not_active Application Discontinuation
- 1991-10-14 SE SE9102975A patent/SE9102975L/xx not_active Application Discontinuation
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