IL305393A - Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof - Google Patents
Recombinant vectors comprising polycistronic expression cassettes and methods of use thereofInfo
- Publication number
- IL305393A IL305393A IL305393A IL30539323A IL305393A IL 305393 A IL305393 A IL 305393A IL 305393 A IL305393 A IL 305393A IL 30539323 A IL30539323 A IL 30539323A IL 305393 A IL305393 A IL 305393A
- Authority
- IL
- Israel
- Prior art keywords
- polynucleotide sequence
- seq
- amino acid
- sequence
- combination
- Prior art date
Links
- 239000013598 vector Substances 0.000 title claims description 210
- 238000000034 method Methods 0.000 title claims description 46
- 102000040430 polynucleotide Human genes 0.000 claims description 1322
- 108091033319 polynucleotide Proteins 0.000 claims description 1322
- 239000002157 polynucleotide Substances 0.000 claims description 1322
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 439
- 150000001413 amino acids Chemical class 0.000 claims description 250
- 239000011575 calcium Substances 0.000 claims description 217
- 108091008874 T cell receptors Proteins 0.000 claims description 187
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims description 160
- 210000004027 cell Anatomy 0.000 claims description 153
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 115
- 108090000623 proteins and genes Proteins 0.000 claims description 86
- 102000004169 proteins and genes Human genes 0.000 claims description 79
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 60
- 230000014509 gene expression Effects 0.000 claims description 41
- 239000012634 fragment Substances 0.000 claims description 40
- 102000008579 Transposases Human genes 0.000 claims description 39
- 108010020764 Transposases Proteins 0.000 claims description 39
- 108090001126 Furin Proteins 0.000 claims description 35
- 102000004961 Furin Human genes 0.000 claims description 34
- 230000004048 modification Effects 0.000 claims description 30
- 238000012986 modification Methods 0.000 claims description 30
- 239000002773 nucleotide Substances 0.000 claims description 26
- 125000003729 nucleotide group Chemical group 0.000 claims description 26
- 239000012642 immune effector Substances 0.000 claims description 18
- 229940121354 immunomodulator Drugs 0.000 claims description 18
- 102000003812 Interleukin-15 Human genes 0.000 claims description 17
- 108090000172 Interleukin-15 Proteins 0.000 claims description 17
- 239000003550 marker Substances 0.000 claims description 17
- 240000007019 Oxalis corniculata Species 0.000 claims description 14
- 239000013256 coordination polymer Substances 0.000 claims description 13
- 108020001507 fusion proteins Proteins 0.000 claims description 13
- 102000037865 fusion proteins Human genes 0.000 claims description 13
- 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 claims description 12
- 102000001301 EGF receptor Human genes 0.000 claims description 12
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 10
- 101100067974 Arabidopsis thaliana POP2 gene Proteins 0.000 claims description 10
- 101100118549 Homo sapiens EGFR gene Proteins 0.000 claims description 10
- 101100123851 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) HER1 gene Proteins 0.000 claims description 10
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 claims description 10
- 210000000130 stem cell Anatomy 0.000 claims description 10
- 239000012636 effector Substances 0.000 claims description 9
- 101000611023 Homo sapiens Tumor necrosis factor receptor superfamily member 6 Proteins 0.000 claims description 8
- 102100040403 Tumor necrosis factor receptor superfamily member 6 Human genes 0.000 claims description 8
- 238000004520 electroporation Methods 0.000 claims description 8
- 206010028980 Neoplasm Diseases 0.000 claims description 7
- 108010047041 Complementarity Determining Regions Proteins 0.000 claims description 6
- 201000011510 cancer Diseases 0.000 claims description 6
- 239000013603 viral vector Substances 0.000 claims description 6
- 101000914514 Homo sapiens T-cell-specific surface glycoprotein CD28 Proteins 0.000 claims description 5
- 102100027213 T-cell-specific surface glycoprotein CD28 Human genes 0.000 claims description 5
- 239000013612 plasmid Substances 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 208000023275 Autoimmune disease Diseases 0.000 claims description 4
- 101001018097 Homo sapiens L-selectin Proteins 0.000 claims description 4
- 102100033467 L-selectin Human genes 0.000 claims description 4
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 4
- 210000003171 tumor-infiltrating lymphocyte Anatomy 0.000 claims description 4
- 210000003630 histaminocyte Anatomy 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 210000003071 memory t lymphocyte Anatomy 0.000 claims description 3
- 210000000581 natural killer T-cell Anatomy 0.000 claims description 3
- 210000001539 phagocyte Anatomy 0.000 claims description 3
- 101001040800 Homo sapiens Integral membrane protein GPR180 Proteins 0.000 claims description 2
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 claims description 2
- 206010033128 Ovarian cancer Diseases 0.000 claims description 2
- 206010061535 Ovarian neoplasm Diseases 0.000 claims description 2
- 102000010292 Peptide Elongation Factor 1 Human genes 0.000 claims description 2
- 108010077524 Peptide Elongation Factor 1 Proteins 0.000 claims description 2
- 239000001506 calcium phosphate Substances 0.000 claims description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 2
- 235000011010 calcium phosphates Nutrition 0.000 claims description 2
- 208000006990 cholangiocarcinoma Diseases 0.000 claims description 2
- 238000001246 colloidal dispersion Methods 0.000 claims description 2
- 231100000433 cytotoxic Toxicity 0.000 claims description 2
- 230000001472 cytotoxic effect Effects 0.000 claims description 2
- 230000003325 follicular Effects 0.000 claims description 2
- 238000001638 lipofection Methods 0.000 claims description 2
- 210000004072 lung Anatomy 0.000 claims description 2
- 238000000520 microinjection Methods 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 238000000527 sonication Methods 0.000 claims description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 798
- 235000001014 amino acid Nutrition 0.000 description 241
- 229940024606 amino acid Drugs 0.000 description 240
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 169
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 169
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 150
- 230000008859 change Effects 0.000 description 66
- 235000018102 proteins Nutrition 0.000 description 53
- 102100030708 GTPase KRas Human genes 0.000 description 45
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 description 45
- 102210042925 HLA-A*02:01 Human genes 0.000 description 33
- 239000012071 phase Substances 0.000 description 27
- 230000009258 tissue cross reactivity Effects 0.000 description 27
- 108020004414 DNA Proteins 0.000 description 25
- 102000004196 processed proteins & peptides Human genes 0.000 description 25
- 229920001184 polypeptide Polymers 0.000 description 24
- 239000000427 antigen Substances 0.000 description 23
- 108091007433 antigens Proteins 0.000 description 23
- 102000036639 antigens Human genes 0.000 description 23
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 19
- 238000013519 translation Methods 0.000 description 14
- 230000014616 translation Effects 0.000 description 14
- 241001529936 Murinae Species 0.000 description 13
- 230000006870 function Effects 0.000 description 12
- 102000004127 Cytokines Human genes 0.000 description 10
- 108090000695 Cytokines Proteins 0.000 description 10
- 238000003501 co-culture Methods 0.000 description 10
- 210000004443 dendritic cell Anatomy 0.000 description 10
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 9
- 108010029485 Protein Isoforms Proteins 0.000 description 9
- 102000001708 Protein Isoforms Human genes 0.000 description 9
- 108700019146 Transgenes Proteins 0.000 description 9
- 108020004999 messenger RNA Proteins 0.000 description 9
- 239000002243 precursor Substances 0.000 description 9
- 239000004471 Glycine Substances 0.000 description 8
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 7
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 7
- 230000001086 cytosolic effect Effects 0.000 description 7
- 238000006467 substitution reaction Methods 0.000 description 7
- 108020004705 Codon Proteins 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 6
- 108010002350 Interleukin-2 Proteins 0.000 description 6
- 125000000174 L-prolyl group Chemical group [H]N1C([H])([H])C([H])([H])C([H])([H])[C@@]1([H])C(*)=O 0.000 description 6
- 125000000510 L-tryptophano group Chemical group [H]C1=C([H])C([H])=C2N([H])C([H])=C(C([H])([H])[C@@]([H])(C(O[H])=O)N([H])[*])C2=C1[H] 0.000 description 6
- 238000011534 incubation Methods 0.000 description 6
- 230000006698 induction Effects 0.000 description 6
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102200006539 rs121913529 Human genes 0.000 description 6
- 102000035160 transmembrane proteins Human genes 0.000 description 6
- 108091005703 transmembrane proteins Proteins 0.000 description 6
- 102100040482 HLA class II histocompatibility antigen, DR beta 3 chain Human genes 0.000 description 5
- 108010061311 HLA-DRB3 Chains Proteins 0.000 description 5
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- 230000004186 co-expression Effects 0.000 description 5
- 239000000539 dimer Substances 0.000 description 5
- 230000007774 longterm Effects 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 5
- 230000028327 secretion Effects 0.000 description 5
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 4
- 108010020515 HLA-A*68 antigen Proteins 0.000 description 4
- 102210009885 HLA-C*08:02 Human genes 0.000 description 4
- 102000043131 MHC class II family Human genes 0.000 description 4
- 108091054438 MHC class II family Proteins 0.000 description 4
- 125000000729 N-terminal amino-acid group Chemical group 0.000 description 4
- 125000000539 amino acid group Chemical group 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 102200006531 rs121913529 Human genes 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 230000001225 therapeutic effect Effects 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 4
- 102210009893 HLA-C*01:02 Human genes 0.000 description 3
- 102000043129 MHC class I family Human genes 0.000 description 3
- 108091054437 MHC class I family Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 3
- 235000018417 cysteine Nutrition 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- BSDCIRGNJKZPFV-GWOFURMSSA-N (2r,3s,4r,5r)-2-(hydroxymethyl)-5-(2,5,6-trichlorobenzimidazol-1-yl)oxolane-3,4-diol Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=CC(Cl)=C(Cl)C=C2N=C1Cl BSDCIRGNJKZPFV-GWOFURMSSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical group [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 description 2
- 102100028972 HLA class I histocompatibility antigen, A alpha chain Human genes 0.000 description 2
- 102100028976 HLA class I histocompatibility antigen, B alpha chain Human genes 0.000 description 2
- 102100028971 HLA class I histocompatibility antigen, C alpha chain Human genes 0.000 description 2
- 108010075704 HLA-A Antigens Proteins 0.000 description 2
- -1 His Chemical compound 0.000 description 2
- 101000851181 Homo sapiens Epidermal growth factor receptor Proteins 0.000 description 2
- 101000763322 Homo sapiens M1-specific T cell receptor beta chain Proteins 0.000 description 2
- 101000763321 Homo sapiens T cell receptor beta chain MC.7.G5 Proteins 0.000 description 2
- 101000662909 Homo sapiens T cell receptor beta constant 1 Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 102100026964 M1-specific T cell receptor beta chain Human genes 0.000 description 2
- 108700005089 MHC Class I Genes Proteins 0.000 description 2
- 101710153660 Nuclear receptor corepressor 2 Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 108091081021 Sense strand Proteins 0.000 description 2
- 102100037272 T cell receptor beta constant 1 Human genes 0.000 description 2
- 241000703392 Tribec virus Species 0.000 description 2
- 238000002441 X-ray diffraction Methods 0.000 description 2
- 238000012867 alanine scanning Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 229910052805 deuterium Inorganic materials 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000027426 receptor tyrosine kinases Human genes 0.000 description 2
- 108091008598 receptor tyrosine kinases Proteins 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000011664 signaling Effects 0.000 description 2
- 238000012916 structural analysis Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000017105 transposition Effects 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 1
- 102100023388 ATP-dependent RNA helicase DHX15 Human genes 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 102000000844 Cell Surface Receptors Human genes 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 108010012236 Chemokines Proteins 0.000 description 1
- 102000019034 Chemokines Human genes 0.000 description 1
- 241001432959 Chernes Species 0.000 description 1
- 108091007741 Chimeric antigen receptor T cells Proteins 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 108060006698 EGF receptor Proteins 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- 102100035233 Furin Human genes 0.000 description 1
- 102000013446 GTP Phosphohydrolases Human genes 0.000 description 1
- 108091006109 GTPases Proteins 0.000 description 1
- 102100028970 HLA class I histocompatibility antigen, alpha chain E Human genes 0.000 description 1
- 102100028966 HLA class I histocompatibility antigen, alpha chain F Human genes 0.000 description 1
- 102100028967 HLA class I histocompatibility antigen, alpha chain G Human genes 0.000 description 1
- 102100029966 HLA class II histocompatibility antigen, DP alpha 1 chain Human genes 0.000 description 1
- 108010058607 HLA-B Antigens Proteins 0.000 description 1
- 108010052199 HLA-C Antigens Proteins 0.000 description 1
- 108010010378 HLA-DP Antigens Proteins 0.000 description 1
- 102000015789 HLA-DP Antigens Human genes 0.000 description 1
- 108010093061 HLA-DPA1 antigen Proteins 0.000 description 1
- 108010062347 HLA-DQ Antigens Proteins 0.000 description 1
- 108010058597 HLA-DR Antigens Proteins 0.000 description 1
- 102000006354 HLA-DR Antigens Human genes 0.000 description 1
- 108010024164 HLA-G Antigens Proteins 0.000 description 1
- 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 1
- 108010027412 Histocompatibility Antigens Class II Proteins 0.000 description 1
- 102000018713 Histocompatibility Antigens Class II Human genes 0.000 description 1
- 101000907886 Homo sapiens ATP-dependent RNA helicase DHX15 Proteins 0.000 description 1
- 101000986084 Homo sapiens HLA class I histocompatibility antigen, C alpha chain Proteins 0.000 description 1
- 101000986085 Homo sapiens HLA class I histocompatibility antigen, alpha chain E Proteins 0.000 description 1
- 101000986080 Homo sapiens HLA class I histocompatibility antigen, alpha chain F Proteins 0.000 description 1
- 101000662902 Homo sapiens T cell receptor beta constant 2 Proteins 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 108700018351 Major Histocompatibility Complex Proteins 0.000 description 1
- 108010050619 Monokines Proteins 0.000 description 1
- 102000013967 Monokines Human genes 0.000 description 1
- 101100096028 Mus musculus Smok1 gene Proteins 0.000 description 1
- 238000005481 NMR spectroscopy Methods 0.000 description 1
- 101100054323 Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) abcC gene Proteins 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 108010003723 Single-Domain Antibodies Proteins 0.000 description 1
- 101150077364 TRDC gene Proteins 0.000 description 1
- 102100023935 Transmembrane glycoprotein NMB Human genes 0.000 description 1
- 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
- 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 125000003295 alanine group Chemical group N[C@@H](C)C(=O)* 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000611 antibody drug conjugate Substances 0.000 description 1
- 230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 1
- 229940049595 antibody-drug conjugate Drugs 0.000 description 1
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002050 diffraction method Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 102000045108 human EGFR Human genes 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000004068 intracellular signaling Effects 0.000 description 1
- 125000000741 isoleucyl group Chemical group [H]N([H])C(C(C([H])([H])[H])C([H])([H])C([H])([H])[H])C(=O)O* 0.000 description 1
- 238000012004 kinetic exclusion assay Methods 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000012900 molecular simulation Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- PTMHPRAIXMAOOB-UHFFFAOYSA-N phosphoramidic acid Chemical compound NP(O)(O)=O PTMHPRAIXMAOOB-UHFFFAOYSA-N 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000037425 regulation of transcription Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 102200006538 rs121913530 Human genes 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 102000030938 small GTPase Human genes 0.000 description 1
- 108060007624 small GTPase Proteins 0.000 description 1
- 230000020382 suppression by virus of host antigen processing and presentation of peptide antigen via MHC class I Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 108091007466 transmembrane glycoproteins Proteins 0.000 description 1
- 125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000002424 x-ray crystallography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5443—IL-15
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K40/00—Cellular immunotherapy
- A61K40/10—Cellular immunotherapy characterised by the cell type used
- A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/7051—T-cell receptor (TcR)-CD3 complex
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Hematology (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Polyethers (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163153862P | 2021-02-25 | 2021-02-25 | |
| US202163159434P | 2021-03-10 | 2021-03-10 | |
| US202163260409P | 2021-08-19 | 2021-08-19 | |
| US202163260855P | 2021-09-02 | 2021-09-02 | |
| US202263267421P | 2022-02-01 | 2022-02-01 | |
| PCT/US2022/070690 WO2022183167A1 (en) | 2021-02-25 | 2022-02-17 | Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL305393A true IL305393A (en) | 2023-10-01 |
Family
ID=80628943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL305393A IL305393A (en) | 2021-02-25 | 2022-02-17 | Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240175047A1 (https=) |
| EP (1) | EP4298121A1 (https=) |
| JP (1) | JP2024507929A (https=) |
| KR (1) | KR20230150336A (https=) |
| AU (1) | AU2022227085A1 (https=) |
| CA (1) | CA3209732A1 (https=) |
| IL (1) | IL305393A (https=) |
| WO (1) | WO2022183167A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4110804A1 (en) * | 2020-02-26 | 2023-01-04 | The United States of America, as represented by the Secretary, Department of Health and Human Services | Hla class ii-restricted t cell receptors against ras with g12v mutation |
| US20250145950A1 (en) * | 2022-02-01 | 2025-05-08 | Alaunos Therapeutics, Inc. | Methods for Activation and Expansion of T Cells |
| US20250216381A1 (en) | 2022-03-21 | 2025-07-03 | Alaunos Therapeutics, Inc. | Methods for Identifying Neoantigen-Reactive T Cell Receptors |
| WO2024059621A2 (en) * | 2022-09-13 | 2024-03-21 | The Trustees Of The University Of Pennsylvania | T-cell receptors and compositions thereof for targeting mutant ras |
| WO2024163371A1 (en) * | 2023-01-30 | 2024-08-08 | Fred Hutchinson Cancer Center | Binding proteins specific for mutant p53 and uses thereof |
| WO2024197072A2 (en) | 2023-03-21 | 2024-09-26 | Alaunos Therapeutics, Inc. | Identification of neoantigen-reactive t cell receptors |
| WO2024206274A2 (en) * | 2023-03-27 | 2024-10-03 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | T cell receptors targeting y220c or r175h mutation in p53 |
| CN117143823B (zh) * | 2023-09-11 | 2024-07-16 | 皖南医学院第一附属医院（皖南医学院弋矶山医院） | 一种基于tcr单链可变区的靶向ras(g12v)的嵌合抗原受体记忆nk细胞的研制及其应用 |
| WO2025056681A1 (en) * | 2023-09-15 | 2025-03-20 | Medigene Immunotherapies Gmbh | T-CELL RECEPTOR AGAINST mKRAS7-16 G12V AND G12C |
| WO2025096983A1 (en) * | 2023-11-02 | 2025-05-08 | Board Of Regents, The University Of Texas System | Peptides and engineered t cell receptors targeting tpx2 antigen and methods of use |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996018105A1 (en) | 1994-12-06 | 1996-06-13 | The President And Fellows Of Harvard College | Single chain t-cell receptor |
| ATE466948T1 (de) | 1997-03-11 | 2010-05-15 | Univ Minnesota | Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle |
| GB9809658D0 (en) | 1998-05-06 | 1998-07-01 | Celltech Therapeutics Ltd | Biological products |
| IL127142A0 (en) | 1998-11-19 | 1999-09-22 | Yeda Res & Dev | Immune cells having predefined biological specificity |
| WO2000063374A1 (en) | 1999-04-16 | 2000-10-26 | Celltech Therapeutics Limited | Synthetic transmembrane components |
| IL160359A0 (en) | 2001-08-31 | 2004-07-25 | Avidex Ltd | Soluble t cell receptor |
| US20040014194A1 (en) | 2002-03-27 | 2004-01-22 | Schering Corporation | Beta-secretase crystals and methods for preparing and using the same |
| WO2003089618A2 (en) | 2002-04-22 | 2003-10-30 | Regents Of The University Of Minnesota | Transposon system and methods of use |
| CA2501870C (en) | 2002-10-09 | 2013-07-02 | Avidex Limited | Single chain recombinant t cell receptors |
| ATE475669T1 (de) | 2004-06-29 | 2010-08-15 | Immunocore Ltd | Einen modifizierten t-zellen-rezeptor exprimierende zellen |
| GB0712670D0 (en) | 2007-06-29 | 2007-08-08 | King S College London | Isolated peptides and uses thereof |
| EP2160461B1 (en) | 2007-07-04 | 2012-08-08 | Max-Delbrück-Centrum für Molekulare Medizin (MDC) | Hyperactive variants of the transposase protein of the transposon system sleeping beauty |
| EP2486049A1 (en) | 2009-10-06 | 2012-08-15 | The Board Of Trustees Of The UniversityOf Illinois | Human single-chain t cell receptors |
| CA3285826A1 (en) | 2012-02-22 | 2026-03-02 | The Trustees Of The University Of Pennsylvania | Compositions and methods for generating a persisting population of t cells useful for the treatment of cancer |
| PT2692865E (pt) | 2012-07-30 | 2015-02-06 | Nbe Therapeutics Llc | Identificação mediada por transposição de proteínas funcionais ou de ligação específicas |
| CN118994363A (zh) | 2014-11-26 | 2024-11-22 | 美国卫生和人力服务部 | 抗突变的kras的t细胞受体 |
| KR102615825B1 (ko) | 2015-03-11 | 2023-12-21 | 더 보드 오브 리젠츠 오브 더 유니버시티 오브 텍사스 시스템 | 전위효소 폴리펩타이드 및 이의 용도 |
| ES2879287T3 (es) | 2015-09-15 | 2021-11-22 | Us Health | Receptores de células T que reconocen KRAS mutado restringido a HLA-cw8 |
| HRP20221183T1 (hr) | 2016-08-02 | 2022-12-09 | The U.S.A. As Represented By The Secretary, Department Of Health And Human Services | T-stanični receptori anti-kras-g12d |
| KR20200003913A (ko) | 2017-05-10 | 2020-01-10 | 이오반스 바이오테라퓨틱스, 인크. | 액상 종양으로부터의 종양 침윤 림프구의 확장 및 그의 치료 용도 |
| CA3065930A1 (en) * | 2017-06-07 | 2018-12-13 | Intrexon Corporation | Expression of novel cell tags |
| EP3673053A1 (en) | 2017-08-21 | 2020-07-01 | EMBL European Molecular Biology Laboratory | Improved transposase polypeptide and uses thereof |
| KR20250037592A (ko) | 2017-09-20 | 2025-03-17 | 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 | 돌연변이된 ras에 대한 hla 클래스 ii-제한된 t 세포 수용체 |
| SG11202002635RA (en) | 2017-09-29 | 2020-04-29 | The United States Of America As Represented By The Secretary | Methods of isolating t cells having antigenic specificity for a p53 cancer-specific mutation |
| WO2019067243A1 (en) | 2017-09-29 | 2019-04-04 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | T CELL RECEPTORS RECOGNIZING P53 MUTE |
| AU2018345400B2 (en) | 2017-10-05 | 2024-06-20 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Methods for selectively expanding cells expressing a TCR with a murine constant region |
| CR20200287A (es) | 2017-12-04 | 2020-11-11 | Us Health | Receptores de células t restringidos a antígeno leucocitario humano (hla) clase i contra sarcoma de rata (ras) mutado |
| US20200399383A1 (en) * | 2018-02-13 | 2020-12-24 | Novartis Ag | Chimeric antigen receptor therapy in combination with il-15r and il15 |
| EP3561053A1 (en) * | 2018-04-26 | 2019-10-30 | Baylor College of Medicine | Immune effector cells and molecular adaptors with an antigen cytokine complex for effective cancer immunotherapy |
| WO2019213195A1 (en) | 2018-05-01 | 2019-11-07 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | T cell receptors which recognize mutated egfr |
| CA3116749A1 (en) | 2018-10-24 | 2020-04-30 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Hla-a3-restricted t cell receptors against mutated ras |
| EP3883955A1 (en) * | 2018-11-19 | 2021-09-29 | Board of Regents, The University of Texas System | A modular, polycistronic vector for car and tcr transduction |
| EP3914612A1 (en) | 2019-01-22 | 2021-12-01 | The United States of America, as represented by the Secretary, Department of Health and Human Services | Hla class ii-restricted t cell receptors against ras with g12r mutation |
| TW202525841A (zh) | 2019-06-27 | 2025-07-01 | 美國衛生與公眾服務部 | 識別p53中之r175h或y220c突變之t細胞受體 |
| CN115279784A (zh) | 2020-02-12 | 2022-11-01 | 美国卫生和人力服务部 | 针对含有g12d突变的ras的hla i类限制性t细胞受体 |
| KR20220143875A (ko) | 2020-02-14 | 2022-10-25 | 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 | G12v 돌연변이를 갖는 ras에 대한 hla 클래스 i-제한된 t 세포 수용체 |
| EP4110804A1 (en) | 2020-02-26 | 2023-01-04 | The United States of America, as represented by the Secretary, Department of Health and Human Services | Hla class ii-restricted t cell receptors against ras with g12v mutation |
-
2022
- 2022-02-17 WO PCT/US2022/070690 patent/WO2022183167A1/en not_active Ceased
- 2022-02-17 JP JP2023551669A patent/JP2024507929A/ja active Pending
- 2022-02-17 KR KR1020237032793A patent/KR20230150336A/ko active Pending
- 2022-02-17 EP EP22708022.3A patent/EP4298121A1/en not_active Withdrawn
- 2022-02-17 IL IL305393A patent/IL305393A/en unknown
- 2022-02-17 CA CA3209732A patent/CA3209732A1/en active Pending
- 2022-02-17 US US18/547,827 patent/US20240175047A1/en active Pending
- 2022-02-17 AU AU2022227085A patent/AU2022227085A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2022227085A1 (en) | 2023-09-14 |
| US20240175047A1 (en) | 2024-05-30 |
| KR20230150336A (ko) | 2023-10-30 |
| JP2024507929A (ja) | 2024-02-21 |
| WO2022183167A1 (en) | 2022-09-01 |
| CA3209732A1 (en) | 2022-09-01 |
| EP4298121A1 (en) | 2024-01-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240175047A1 (en) | Recombinant Vectors Comprising Polycistronic Expression Cassettes and Methods of Use Thereof | |
| JP7379561B2 (ja) | キメラ抗原及びｔ細胞受容体、並びに使用方法 | |
| EP3481867B1 (en) | Programmed death 1 ligand 1 (pd-l1) binding proteins and methods of use thereof | |
| BR112021003305A2 (pt) | métodos para produzir células que expressam receptor de antígeno quimérico | |
| KR20200071079A (ko) | 공동-자극을 위한 신규한 플랫폼, 신규한 car 설계 및 입양 세포 치료를 위한 다른 향상 | |
| JP2022514023A (ja) | 操作されたサイトカイン受容体対を使用して腫瘍浸潤リンパ球を拡大培養する方法及びその使用 | |
| AU2016238963A1 (en) | Method and compositions for cellular immunotherapy | |
| JP7627080B2 (ja) | Ｂ７ｈ３キメラ抗原受容体を含む方法および組成物 | |
| TW202039831A (zh) | 對抗ｐｄ－１抗體呈現難治性之非小細胞肺癌（ｎｓｃｌｃ）病患之治療 | |
| BR112019017008A2 (pt) | anticorpo, ácido nucleico, vetor de expressão, célula hospedeira, imunoconjugado multifuncional, receptor de antígeno quimérico, vírus, célula imune modificada pelo receptor do antígeno quimérico, composição farmacêutica, kit, e, uso do anticorpo. | |
| JP2022547837A (ja) | 遺伝子操作されたｔ細胞及びその産生方法 | |
| CA3237016A1 (en) | Treatment of cancer with nk cells and multispecific engagers | |
| AU2022254689A1 (en) | Treatment of cancer with nk cells and a her2 targeted antibody | |
| CN116113689A (zh) | 改善免疫细胞功能 | |
| TW202323521A (zh) | 製備表現嵌合抗原受體的細胞之方法 | |
| CA3107515A1 (en) | Method for tumor treatment with immune effector cells | |
| JP2024510162A (ja) | 免疫細胞機能の改善 | |
| JPWO2018143454A1 (ja) | 新規ｔ細胞受容体 | |
| WO2022206994A1 (en) | Engineered immune cells and uses thereof | |
| US20250145950A1 (en) | Methods for Activation and Expansion of T Cells | |
| CN118541160A (zh) | 通过促进优越的适应性免疫细胞群体增强过继细胞转移 | |
| WO2022179613A1 (en) | Engineered immune effector cells expressing exogenously introduced cytokines | |
| CN117098772A (zh) | 包含多顺反子表达盒的重组载体及其使用方法 | |
| HK40101165A (zh) | 工程化的免疫细胞及其用途 | |
| HK40008962A (en) | Programmed death 1 ligand 1 (pd-l1) binding proteins and methods of use thereof |