IL223864B - Methods for producing a heterologous polypeptide in recombinant bacterial cells and bacterial cells produced thereby - Google Patents

Methods for producing a heterologous polypeptide in recombinant bacterial cells and bacterial cells produced thereby

Info

Publication number
IL223864B
IL223864B IL223864A IL22386412A IL223864B IL 223864 B IL223864 B IL 223864B IL 223864 A IL223864 A IL 223864A IL 22386412 A IL22386412 A IL 22386412A IL 223864 B IL223864 B IL 223864B
Authority
IL
Israel
Prior art keywords
bacterial cells
producing
methods
heterologous polypeptide
recombinant
Prior art date
Application number
IL223864A
Other languages
English (en)
Hebrew (he)
Original Assignee
Lonza Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP10171252A external-priority patent/EP2412813A1/de
Application filed by Lonza Ag filed Critical Lonza Ag
Publication of IL223864B publication Critical patent/IL223864B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IL223864A 2010-07-29 2012-12-25 Methods for producing a heterologous polypeptide in recombinant bacterial cells and bacterial cells produced thereby IL223864B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10171252A EP2412813A1 (de) 2010-07-29 2010-07-29 Regulierung von induzierbaren Promotoren
EP11162420 2011-04-14
PCT/EP2011/062921 WO2012013710A2 (en) 2010-07-29 2011-07-27 Regulation of inducible promoters

Publications (1)

Publication Number Publication Date
IL223864B true IL223864B (en) 2018-05-31

Family

ID=44533478

Family Applications (1)

Application Number Title Priority Date Filing Date
IL223864A IL223864B (en) 2010-07-29 2012-12-25 Methods for producing a heterologous polypeptide in recombinant bacterial cells and bacterial cells produced thereby

Country Status (18)

Country Link
US (1) US9464294B2 (de)
EP (2) EP2598640B1 (de)
JP (1) JP6071878B2 (de)
KR (2) KR101904140B1 (de)
CN (2) CN103108954B (de)
AU (1) AU2011284844B9 (de)
BR (1) BR112013003657A2 (de)
CA (1) CA2803654C (de)
DK (1) DK2722390T3 (de)
EA (2) EA034074B1 (de)
ES (1) ES2636910T3 (de)
IL (1) IL223864B (de)
MX (1) MX352068B (de)
PL (1) PL2722390T3 (de)
SG (1) SG187024A1 (de)
TW (2) TWI510623B (de)
WO (1) WO2012013710A2 (de)
ZA (1) ZA201209268B (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9970017B2 (en) * 2013-03-27 2018-05-15 Lonza Ag Regulation of inducible promoters
CN104404135B (zh) * 2014-10-27 2017-12-29 首都师范大学 实时荧光定量PCR定位基因转录起始位点和PolyA位点的方法
KR102239307B1 (ko) * 2020-06-29 2021-04-12 한국생명공학연구원 포도당에 의해 조절되는 프로모터 변이체 및 이를 이용한 단백질 생산 방법
CN112062822B (zh) * 2020-09-24 2022-02-01 江南大学 一种碳分解代谢调控蛋白CcpA突变体I42A

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5176114B2 (ja) 2005-06-06 2013-04-03 フェネックス インコーポレイテッド 細菌宿主細胞におけるマンニトール誘導のプロモーター系
EP2284273B1 (de) * 2009-08-10 2012-10-10 Lonza AG Vektor mit Mannose-Promoter und Mannose-Promoter
EP2292769A1 (de) * 2009-08-10 2011-03-09 Lonza AG Fermentierungsverfahren

Also Published As

Publication number Publication date
US20130203114A1 (en) 2013-08-08
MX2013001037A (es) 2013-04-03
EA034074B1 (ru) 2019-12-25
TW201213544A (en) 2012-04-01
EP2722390B1 (de) 2017-05-10
JP2013535197A (ja) 2013-09-12
KR101904140B1 (ko) 2018-10-04
CN105200100A (zh) 2015-12-30
DK2722390T3 (en) 2017-08-28
CA2803654A1 (en) 2012-02-02
CN103108954B (zh) 2015-05-13
AU2011284844A1 (en) 2013-01-10
US9464294B2 (en) 2016-10-11
KR20130054328A (ko) 2013-05-24
PL2722390T3 (pl) 2017-12-29
EA024834B1 (ru) 2016-10-31
EA201600029A1 (ru) 2016-05-31
KR20180086261A (ko) 2018-07-30
EP2598640A2 (de) 2013-06-05
ES2636910T3 (es) 2017-10-10
EA201300189A1 (ru) 2013-05-30
HK1184496A1 (en) 2014-01-24
CA2803654C (en) 2021-02-16
WO2012013710A3 (en) 2012-04-26
TW201428102A (zh) 2014-07-16
EP2722390A1 (de) 2014-04-23
MX352068B (es) 2017-11-08
EP2598640B1 (de) 2015-04-01
WO2012013710A2 (en) 2012-02-02
TWI510623B (zh) 2015-12-01
KR102022884B1 (ko) 2019-09-20
SG187024A1 (en) 2013-02-28
BR112013003657A2 (pt) 2017-02-21
ZA201209268B (en) 2013-07-31
AU2011284844B9 (en) 2015-10-29
CN103108954A (zh) 2013-05-15
JP6071878B2 (ja) 2017-02-01
AU2011284844B2 (en) 2015-10-15
TWI515295B (zh) 2016-01-01

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