IL201622A - Insect resistant plant - Google Patents
Insect resistant plantInfo
- Publication number
- IL201622A IL201622A IL201622A IL20162209A IL201622A IL 201622 A IL201622 A IL 201622A IL 201622 A IL201622 A IL 201622A IL 20162209 A IL20162209 A IL 20162209A IL 201622 A IL201622 A IL 201622A
- Authority
- IL
- Israel
- Prior art keywords
- seq
- primer
- plant
- qtl
- marker
- Prior art date
Links
- 241000238631 Hexapoda Species 0.000 title claims description 69
- 239000013615 primer Substances 0.000 claims description 1366
- 239000003550 marker Substances 0.000 claims description 827
- 241000196324 Embryophyta Species 0.000 claims description 497
- 230000002441 reversible effect Effects 0.000 claims description 406
- 241000254123 Bemisia Species 0.000 claims description 312
- 241001414989 Thysanoptera Species 0.000 claims description 266
- 210000000349 chromosome Anatomy 0.000 claims description 221
- 240000004160 Capsicum annuum Species 0.000 claims description 207
- 108700028369 Alleles Proteins 0.000 claims description 193
- 238000000034 method Methods 0.000 claims description 110
- 230000002068 genetic effect Effects 0.000 claims description 105
- 206010061217 Infestation Diseases 0.000 claims description 90
- 239000002987 primer (paints) Substances 0.000 claims description 89
- 235000002566 Capsicum Nutrition 0.000 claims description 86
- 239000003155 DNA primer Substances 0.000 claims description 84
- 108091034117 Oligonucleotide Proteins 0.000 claims description 73
- 235000013399 edible fruits Nutrition 0.000 claims description 66
- 238000003556 assay Methods 0.000 claims description 58
- 230000003321 amplification Effects 0.000 claims description 53
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 53
- 238000012408 PCR amplification Methods 0.000 claims description 44
- 230000002596 correlated effect Effects 0.000 claims description 44
- 125000003729 nucleotide group Chemical group 0.000 claims description 44
- 239000006002 Pepper Substances 0.000 claims description 43
- 235000016761 Piper aduncum Nutrition 0.000 claims description 43
- 235000017804 Piper guineense Nutrition 0.000 claims description 43
- 235000008184 Piper nigrum Nutrition 0.000 claims description 43
- 239000001511 capsicum annuum Substances 0.000 claims description 42
- 239000002773 nucleotide Substances 0.000 claims description 42
- 235000002567 Capsicum annuum Nutrition 0.000 claims description 41
- 238000006243 chemical reaction Methods 0.000 claims description 39
- 230000001747 exhibiting effect Effects 0.000 claims description 38
- 239000001390 capsicum minimum Substances 0.000 claims description 31
- 150000007523 nucleic acids Chemical class 0.000 claims description 31
- 108020004707 nucleic acids Proteins 0.000 claims description 26
- 102000039446 nucleic acids Human genes 0.000 claims description 26
- 238000012216 screening Methods 0.000 claims description 26
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 19
- 230000002349 favourable effect Effects 0.000 claims description 19
- 230000007613 environmental effect Effects 0.000 claims description 15
- 230000035772 mutation Effects 0.000 claims description 15
- OCKGFTQIICXDQW-ZEQRLZLVSA-N 5-[(1r)-1-hydroxy-2-[4-[(2r)-2-hydroxy-2-(4-methyl-1-oxo-3h-2-benzofuran-5-yl)ethyl]piperazin-1-yl]ethyl]-4-methyl-3h-2-benzofuran-1-one Chemical compound C1=C2C(=O)OCC2=C(C)C([C@@H](O)CN2CCN(CC2)C[C@H](O)C2=CC=C3C(=O)OCC3=C2C)=C1 OCKGFTQIICXDQW-ZEQRLZLVSA-N 0.000 claims description 13
- 239000003147 molecular marker Substances 0.000 claims description 11
- 230000006798 recombination Effects 0.000 claims description 11
- 238000005215 recombination Methods 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 10
- 230000001364 causal effect Effects 0.000 claims description 9
- 238000003306 harvesting Methods 0.000 claims description 8
- 238000010561 standard procedure Methods 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 3
- 230000010153 self-pollination Effects 0.000 claims description 3
- 241000722363 Piper Species 0.000 claims 17
- 241000208293 Capsicum Species 0.000 claims 6
- 230000001276 controlling effect Effects 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 description 103
- 240000003889 Piper guineense Species 0.000 description 87
- 108020004414 DNA Proteins 0.000 description 55
- 240000008574 Capsicum frutescens Species 0.000 description 41
- 108090000623 proteins and genes Proteins 0.000 description 40
- 239000000523 sample Substances 0.000 description 36
- 238000012360 testing method Methods 0.000 description 35
- 238000009396 hybridization Methods 0.000 description 34
- 230000001488 breeding effect Effects 0.000 description 31
- 238000009395 breeding Methods 0.000 description 28
- 108091028043 Nucleic acid sequence Proteins 0.000 description 27
- 238000011161 development Methods 0.000 description 23
- 230000018109 developmental process Effects 0.000 description 23
- 230000000694 effects Effects 0.000 description 20
- 238000013507 mapping Methods 0.000 description 19
- 210000001519 tissue Anatomy 0.000 description 16
- 238000013459 approach Methods 0.000 description 15
- 239000012634 fragment Substances 0.000 description 15
- 241000607479 Yersinia pestis Species 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 14
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 12
- 241000758706 Piperaceae Species 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 12
- 241000927584 Frankliniella occidentalis Species 0.000 description 11
- 108091092878 Microsatellite Proteins 0.000 description 10
- 241000016010 Tomato spotted wilt orthotospovirus Species 0.000 description 10
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Chemical class Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 10
- 241000254127 Bemisia tabaci Species 0.000 description 9
- 244000052769 pathogen Species 0.000 description 9
- 230000001717 pathogenic effect Effects 0.000 description 9
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 8
- 241000723873 Tobacco mosaic virus Species 0.000 description 8
- 230000000875 corresponding effect Effects 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 238000011081 inoculation Methods 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 235000002568 Capsicum frutescens Nutrition 0.000 description 7
- 240000001980 Cucurbita pepo Species 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 7
- 210000001339 epidermal cell Anatomy 0.000 description 7
- 230000017448 oviposition Effects 0.000 description 7
- 235000009854 Cucurbita moschata Nutrition 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 6
- 230000008654 plant damage Effects 0.000 description 6
- 108091033319 polynucleotide Proteins 0.000 description 6
- 102000040430 polynucleotide Human genes 0.000 description 6
- 239000002157 polynucleotide Substances 0.000 description 6
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 235000009852 Cucurbita pepo Nutrition 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 102000054765 polymorphisms of proteins Human genes 0.000 description 5
- 235000020354 squash Nutrition 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 210000001161 mammalian embryo Anatomy 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 238000005204 segregation Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 238000002054 transplantation Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 241000078491 Almeria Species 0.000 description 3
- 102000004150 Flap endonucleases Human genes 0.000 description 3
- 108090000652 Flap endonucleases Proteins 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 235000013601 eggs Nutrition 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000010152 pollination Effects 0.000 description 3
- 210000001938 protoplast Anatomy 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- 241000272165 Charadriidae Species 0.000 description 2
- 241000219112 Cucumis Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 241000189565 Frankliniella Species 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 2
- 241001247145 Sebastes goodei Species 0.000 description 2
- 240000005319 Sedum acre Species 0.000 description 2
- 235000014327 Sedum acre Nutrition 0.000 description 2
- 241000208292 Solanaceae Species 0.000 description 2
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 2
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 2
- 241000189579 Thripidae Species 0.000 description 2
- 241000339374 Thrips tabaci Species 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000007405 data analysis Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 238000013401 experimental design Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000002875 fluorescence polarization Methods 0.000 description 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 2
- 230000005078 fruit development Effects 0.000 description 2
- 102000054766 genetic haplotypes Human genes 0.000 description 2
- 238000003205 genotyping method Methods 0.000 description 2
- 238000009399 inbreeding Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000005070 ripening Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- 241000254124 Aleyrodidae Species 0.000 description 1
- 241001136792 Alle Species 0.000 description 1
- 241000406588 Amblyseius Species 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 241001302798 Bemisia argentifolii Species 0.000 description 1
- 235000002283 Capsicum annuum var aviculare Nutrition 0.000 description 1
- 235000013303 Capsicum annuum var. frutescens Nutrition 0.000 description 1
- 235000002284 Capsicum baccatum var baccatum Nutrition 0.000 description 1
- 235000018306 Capsicum chinense Nutrition 0.000 description 1
- 244000185501 Capsicum chinense Species 0.000 description 1
- 241000324343 Causa Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
- 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 241000275449 Diplectrum formosum Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 101100156281 Drosophila melanogaster viaf gene Proteins 0.000 description 1
- 108091092584 GDNA Proteins 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241000023813 Isia Species 0.000 description 1
- 241000123059 Lutjanus jocu Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000302512 Momordica charantia Species 0.000 description 1
- 235000009811 Momordica charantia Nutrition 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091005461 Nucleic proteins Chemical group 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 241001635529 Orius Species 0.000 description 1
- 241000713112 Orthobunyavirus Species 0.000 description 1
- 241000712894 Orthotospovirus Species 0.000 description 1
- 241000150350 Peribunyaviridae Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 241000365764 Scirtothrips dorsalis Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241000592344 Spermatophyta Species 0.000 description 1
- 241000339373 Thrips palmi Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 229940004511 androxy Drugs 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- 238000000098 azimuthal photoelectron diffraction Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000001728 capsicum frutescens Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 238000003967 crop rotation Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000013213 extrapolation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000004920 integrated pest control Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000008775 paternal effect Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000009394 selective breeding Methods 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 238000007492 two-way ANOVA Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
- A01H6/822—Capsicum sp. [pepper]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/08—Fruits
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07290556 | 2007-05-02 | ||
| EP07119649 | 2007-10-30 | ||
| PCT/EP2008/055374 WO2008135510A1 (en) | 2007-05-02 | 2008-04-30 | Insect resistant plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL201622A0 IL201622A0 (en) | 2010-05-31 |
| IL201622A true IL201622A (en) | 2014-05-28 |
Family
ID=39729778
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL201622A IL201622A (en) | 2007-05-02 | 2009-10-19 | Insect resistant plant |
| IL226355A IL226355A (en) | 2007-05-02 | 2013-05-13 | Insect resistant plant |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL226355A IL226355A (en) | 2007-05-02 | 2013-05-13 | Insect resistant plant |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US8993855B2 (enExample) |
| EP (2) | EP2543741A1 (enExample) |
| JP (1) | JP2010526529A (enExample) |
| KR (1) | KR101202971B1 (enExample) |
| CN (1) | CN101730750B (enExample) |
| AU (1) | AU2008248636B2 (enExample) |
| BR (1) | BRPI0811174B8 (enExample) |
| CA (1) | CA2685809C (enExample) |
| ES (1) | ES2424439T5 (enExample) |
| IL (2) | IL201622A (enExample) |
| MX (1) | MX2009011669A (enExample) |
| RU (2) | RU2522477C2 (enExample) |
| WO (1) | WO2008135510A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8471113B2 (en) * | 2010-12-10 | 2013-06-25 | Seminis Vegetable Seeds, Inc. | Pepper hybrid PS09954859 |
| US8455738B2 (en) * | 2010-12-10 | 2013-06-04 | Seminis Vegetable Seeds, Inc. | Pepper hybrid PS09956434 |
| US8304631B2 (en) * | 2010-12-10 | 2012-11-06 | Seminis Vegetable Seeds, Inc. | Pepper hybrid PS09943431 |
| US8455737B2 (en) * | 2010-12-10 | 2013-06-04 | Seminis Vegetable Seeds, Inc. | Pepper hybrid PS09967422 |
| EP2653029A1 (en) * | 2012-04-19 | 2013-10-23 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Thrips resistant cabbage |
| EP2966989A1 (en) * | 2013-03-15 | 2016-01-20 | Rijk Zwaan Zaadteelt en Zaadhandel B.V. | Resistance against leveillula taurica in pepper |
| US10307889B2 (en) * | 2015-03-30 | 2019-06-04 | 3M Innovative Properties Company | Coated abrasive article and method of making the same |
| EP3557982A1 (en) | 2016-12-21 | 2019-10-30 | Syngenta Participations AG | Prolific flowering watermelon |
| WO2018200550A1 (en) * | 2017-04-26 | 2018-11-01 | Seminis Vegetable Seeds, Inc. | Pepper plants with improved pest resistance |
| CN110149947B (zh) * | 2019-06-24 | 2021-11-30 | 云南省烟草公司文山州公司 | 一种烟草田间抗蓟马性状的筛选研究方法 |
| CN110499390B (zh) * | 2019-09-29 | 2023-03-10 | 云南省烟草农业科学研究院 | 用于烟草抗斑萎病rtsw基因辅助选择的分子标记引物、辅助选择的方法及其应用 |
| MX2022015808A (es) | 2020-06-10 | 2023-01-24 | Nunhems Bv | Plantas de capsicum annuum con resistencia mejorada a los tisanopteros. |
| CN113862386B (zh) * | 2021-08-16 | 2023-11-03 | 中国农业科学院蔬菜花卉研究所 | 一种与美洲南瓜Cucurbita pepo L.叶片缺刻基因Cpdll连锁的Indel标记及其引物、试剂盒与应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| RU2131181C1 (ru) * | 1997-07-15 | 1999-06-10 | Фурсов Виктор Николаевич | Способ селекции культурного хлопчатника с ранним естественным листоопадением, устойчивостью против белокрылки и экологически чистым снежно-белым волокном |
| MXPA02008316A (es) | 2000-02-25 | 2004-09-10 | Dna Plant Techn Corp | Nueva variedad de pimientos. |
| IL157538A0 (en) * | 2003-08-21 | 2004-03-28 | Bar Ilan Res & Dev Company Ltd | Plant resistant to cytoplasm-feeding parasites |
| PT2316261T (pt) * | 2004-02-12 | 2021-01-06 | Seminis Vegetable Seeds Inc | Métodos para acoplar alelos de resistência em tomates |
| CN1817104A (zh) * | 2006-03-28 | 2006-08-16 | 江苏辉丰农化股份有限公司 | 一种利用农作物杂种优势进行育种的方法 |
-
2008
- 2008-04-30 WO PCT/EP2008/055374 patent/WO2008135510A1/en not_active Ceased
- 2008-04-30 JP JP2010504748A patent/JP2010526529A/ja active Pending
- 2008-04-30 AU AU2008248636A patent/AU2008248636B2/en active Active
- 2008-04-30 US US12/451,236 patent/US8993855B2/en active Active
- 2008-04-30 MX MX2009011669A patent/MX2009011669A/es active IP Right Grant
- 2008-04-30 CN CN2008800232660A patent/CN101730750B/zh active Active
- 2008-04-30 CA CA2685809A patent/CA2685809C/en active Active
- 2008-04-30 EP EP12183382A patent/EP2543741A1/en not_active Withdrawn
- 2008-04-30 EP EP08749952.1A patent/EP2140023B9/en active Active
- 2008-04-30 RU RU2009144326/10A patent/RU2522477C2/ru active
- 2008-04-30 KR KR1020097022928A patent/KR101202971B1/ko active Active
- 2008-04-30 BR BRPI0811174A patent/BRPI0811174B8/pt active IP Right Grant
- 2008-04-30 ES ES08749952T patent/ES2424439T5/es active Active
-
2009
- 2009-10-19 IL IL201622A patent/IL201622A/en active IP Right Grant
-
2013
- 2013-05-13 IL IL226355A patent/IL226355A/en active IP Right Grant
-
2014
- 2014-01-17 RU RU2014101369A patent/RU2617955C2/ru active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2140023B1 (en) | 2013-05-08 |
| JP2010526529A (ja) | 2010-08-05 |
| BRPI0811174B8 (pt) | 2020-06-09 |
| EP2543741A1 (en) | 2013-01-09 |
| WO2008135510A1 (en) | 2008-11-13 |
| US8993855B2 (en) | 2015-03-31 |
| IL226355A0 (en) | 2013-06-27 |
| EP2140023B9 (en) | 2024-06-19 |
| IL226355A (en) | 2014-12-31 |
| CA2685809A1 (en) | 2008-11-13 |
| BRPI0811174B1 (pt) | 2020-05-26 |
| EP2140023A1 (en) | 2010-01-06 |
| IL201622A0 (en) | 2010-05-31 |
| BRPI0811174A2 (pt) | 2014-12-23 |
| EP2140023B2 (en) | 2024-01-31 |
| RU2009144326A (ru) | 2011-06-10 |
| US20100122383A1 (en) | 2010-05-13 |
| RU2522477C2 (ru) | 2014-07-20 |
| RU2617955C2 (ru) | 2017-04-28 |
| AU2008248636A1 (en) | 2008-11-13 |
| AU2008248636B2 (en) | 2013-10-03 |
| RU2014101369A (ru) | 2015-07-27 |
| HK1139713A1 (en) | 2010-09-24 |
| CN101730750A (zh) | 2010-06-09 |
| MX2009011669A (es) | 2009-11-10 |
| ES2424439T3 (es) | 2013-10-02 |
| CN101730750B (zh) | 2013-07-10 |
| ES2424439T5 (es) | 2024-08-06 |
| CA2685809C (en) | 2017-05-23 |
| KR20090126321A (ko) | 2009-12-08 |
| KR101202971B1 (ko) | 2012-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8993855B2 (en) | Insect resistant plant | |
| JP5405455B2 (ja) | 改良されたペッパー植物 | |
| US8975468B2 (en) | Disease resistant tomato plants | |
| US9137959B2 (en) | Insect resistant plant | |
| HK1180729A (en) | Insect resistant plant | |
| HK1139713B (en) | Insect resistant plant | |
| HK1134994B (en) | Improved pepper plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |