IL136190A - A method for detecting cancer cells - Google Patents
A method for detecting cancer cellsInfo
- Publication number
- IL136190A IL136190A IL13619098A IL13619098A IL136190A IL 136190 A IL136190 A IL 136190A IL 13619098 A IL13619098 A IL 13619098A IL 13619098 A IL13619098 A IL 13619098A IL 136190 A IL136190 A IL 136190A
- Authority
- IL
- Israel
- Prior art keywords
- dyes
- image
- sample
- pixels
- concentrations
- Prior art date
Links
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Classifications
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Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
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- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/984,990 US5995645A (en) | 1993-08-18 | 1997-12-04 | Method of cancer cell detection |
| PCT/US1998/025312 WO1999028856A1 (en) | 1997-12-04 | 1998-11-27 | Method of cancer cell detection |
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| Publication Number | Publication Date |
|---|---|
| IL136190A0 IL136190A0 (en) | 2001-05-20 |
| IL136190A true IL136190A (en) | 2004-02-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| IL13619098A IL136190A (en) | 1997-12-04 | 1998-11-27 | A method for detecting cancer cells |
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| US (1) | US5995645A (ja) |
| EP (1) | EP1042727A4 (ja) |
| JP (1) | JP2001525580A (ja) |
| AU (1) | AU1610299A (ja) |
| IL (1) | IL136190A (ja) |
| WO (1) | WO1999028856A1 (ja) |
Families Citing this family (157)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6514722B2 (en) | 1997-03-27 | 2003-02-04 | Oncosis | Method and apparatus for selectively targeting specific cells within a cell population |
| US6753161B2 (en) * | 1997-03-27 | 2004-06-22 | Oncosis Llc | Optoinjection methods |
| US20080241848A1 (en) * | 1998-05-09 | 2008-10-02 | Ikonisys, Inc. | Methods for prenatal diagnosis of aneuploidy |
| US20090111101A1 (en) * | 1998-05-09 | 2009-04-30 | Ikonisys, Inc. | Automated Cancer Diagnostic Methods Using FISH |
| US7901887B2 (en) * | 1998-05-09 | 2011-03-08 | Ikonisys, Inc. | Automated cancer diagnostic methods using fish |
| CN1702459A (zh) | 1998-05-09 | 2005-11-30 | 伊康尼西斯公司 | 用于包括胎儿细胞的计算机所控制的稀少细胞的基于诊断的方法和装置 |
| CA2356623C (en) | 1998-12-23 | 2005-10-18 | Medispectra, Inc. | Systems and methods for optical examination of samples |
| US8229549B2 (en) | 2004-07-09 | 2012-07-24 | Tyco Healthcare Group Lp | Surgical imaging device |
| EP1553402A3 (en) * | 1999-07-30 | 2005-08-10 | California Institute Of Technology | System and method for monitoring cellular activity |
| US6750964B2 (en) * | 1999-08-06 | 2004-06-15 | Cambridge Research And Instrumentation, Inc. | Spectral imaging methods and systems |
| GB2355521A (en) * | 1999-09-30 | 2001-04-25 | Marconi Avionics | An imaging system |
| US7260248B2 (en) * | 1999-12-15 | 2007-08-21 | Medispectra, Inc. | Image processing using measures of similarity |
| JP4731698B2 (ja) * | 2000-04-06 | 2011-07-27 | キヤノン株式会社 | 画像処理装置、撮影装置、画像処理システム、画像処理方法、及び記憶媒体 |
| DE60137030D1 (de) | 2000-04-20 | 2009-01-29 | Greenvision Systems Ltd | Verfahren zur erzeugung von morphologischen konzentrations-/dichteabbildungen und histogrammen in partikeln einer chemisch reinen partikelsubstanz |
| CN1741036A (zh) | 2000-06-19 | 2006-03-01 | 科雷洛吉克系统公司 | 构造分类属于不同状态的生物样本的模型的方法 |
| US6925389B2 (en) * | 2000-07-18 | 2005-08-02 | Correlogic Systems, Inc., | Process for discriminating between biological states based on hidden patterns from biological data |
| AU8475301A (en) * | 2000-08-07 | 2002-02-18 | Univ Vanderbilt | Detection of cox-2 activity and anandamide metabolites |
| JP2004512845A (ja) | 2000-10-24 | 2004-04-30 | オンコシス リミテッド ライアビリティ カンパニー | 三次元の検体内の細胞を選択的に標的化する方法およびデバイス |
| JP4241038B2 (ja) | 2000-10-30 | 2009-03-18 | ザ ジェネラル ホスピタル コーポレーション | 組織分析のための光学的な方法及びシステム |
| FR2817986B1 (fr) * | 2000-12-07 | 2003-03-28 | Lyon Ecole Centrale | Procede de classification d'une image en couleur selon la prise de vue en exterieur ou en interieur |
| US6839661B2 (en) * | 2000-12-15 | 2005-01-04 | Medispectra, Inc. | System for normalizing spectra |
| JP4617059B2 (ja) | 2001-04-20 | 2011-01-19 | パワー メディカル インターベンションズ， エルエルシー | イメージング装置 |
| US9897538B2 (en) | 2001-04-30 | 2018-02-20 | The General Hospital Corporation | Method and apparatus for improving image clarity and sensitivity in optical coherence tomography using dynamic feedback to control focal properties and coherence gating |
| WO2002099734A1 (en) * | 2001-06-04 | 2002-12-12 | Ikonisys Inc. | Automated image analysis for detecting microalgae and other organisms in the environment |
| US20020169730A1 (en) * | 2001-08-29 | 2002-11-14 | Emmanuel Lazaridis | Methods for classifying objects and identifying latent classes |
| ES2361151T3 (es) | 2002-01-30 | 2011-06-14 | Tyco Healthcare Group Lp | Dispositivo quirúrgico de formación de imagen. |
| US20040082863A1 (en) * | 2002-03-15 | 2004-04-29 | Mcgreevy James | Device and method for the photodynamic diagnosis of tumor tissue |
| US6818903B2 (en) * | 2002-07-09 | 2004-11-16 | Medispectra, Inc. | Method and apparatus for identifying spectral artifacts |
| US6768918B2 (en) | 2002-07-10 | 2004-07-27 | Medispectra, Inc. | Fluorescent fiberoptic probe for tissue health discrimination and method of use thereof |
| TW200403434A (en) * | 2002-07-29 | 2004-03-01 | Correlogic Systems Inc | Quality assurance/quality control for electrospray ionization processes |
| US7314709B2 (en) * | 2002-08-06 | 2008-01-01 | Vanderbilt University | Compositions and methods for detecting and quantifying COX-2 activity by lipoamino acid metabolism |
| CN101904734B (zh) | 2002-09-30 | 2013-01-02 | Tyco医疗健康集团 | 整装的可灭菌外科系统 |
| AU2003273668A1 (en) * | 2002-10-02 | 2004-04-23 | Ifire Technology Corp.Lumen Health Innovations, Inc. | Apparatus and methods relating to high speed spectroscopy and excitation-emission matrices |
| US7351574B2 (en) | 2002-11-27 | 2008-04-01 | 3M Innovative Properties Company | Loading and ejection systems for biological growth plate scanner |
| US7298885B2 (en) * | 2002-11-27 | 2007-11-20 | 3M Innovative Properties Company | Biological growth plate scanner with automated image processing profile selection |
| US20040101954A1 (en) * | 2002-11-27 | 2004-05-27 | Graessle Josef A. | Back side plate illumination for biological growth plate scanner |
| US7319031B2 (en) | 2002-11-27 | 2008-01-15 | 3M Innovative Properties Company | Mounting platform for biological growth plate scanner |
| US20040102903A1 (en) * | 2002-11-27 | 2004-05-27 | Graessle Josef A. | Biological growth plate scanner |
| AU2004225188B2 (en) | 2003-03-31 | 2010-04-15 | The General Hospital Corporation | Speckle reduction in optical coherence tomography by path length encoded angular compounding |
| WO2004111930A2 (en) * | 2003-05-30 | 2004-12-23 | Rabinovich Andrew M | Color unmixing and region of interest detection in tissue samples |
| US7519096B2 (en) | 2003-06-06 | 2009-04-14 | The General Hospital Corporation | Process and apparatus for a wavelength tuning source |
| WO2005011474A2 (en) * | 2003-08-01 | 2005-02-10 | Correlogic Systems, Inc. | Multiple high-resolution serum proteomic features for ovarian cancer detection |
| US7496225B2 (en) * | 2003-09-04 | 2009-02-24 | 3M Innovative Properties Company | Biological growth plate scanner with automated intake |
| US7298886B2 (en) | 2003-09-05 | 2007-11-20 | 3M Innovative Properties Company | Counting biological agents on biological growth plates |
| US7321791B2 (en) | 2003-09-23 | 2008-01-22 | Cambridge Research And Instrumentation, Inc. | Spectral imaging of deep tissue |
| US8634607B2 (en) * | 2003-09-23 | 2014-01-21 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
| US20050095578A1 (en) * | 2003-10-31 | 2005-05-05 | Koller Manfred R. | Method and apparatus for cell permeabilization |
| US7425426B2 (en) | 2004-03-15 | 2008-09-16 | Cyntellect, Inc. | Methods for purification of cells based on product secretion |
| JP4995720B2 (ja) | 2004-07-02 | 2012-08-08 | ザ ジェネラル ホスピタル コーポレイション | ダブルクラッドファイバを有する内視鏡撮像プローブ |
| EP1989997A1 (en) | 2004-08-24 | 2008-11-12 | The General Hospital Corporation | Process, System and Software Arrangement for Measuring a Mechanical Strain and Elastic Properties of a Sample |
| KR20120062944A (ko) | 2004-08-24 | 2012-06-14 | 더 제너럴 하스피탈 코포레이션 | 혈관절편 영상화 방법 및 장치 |
| CA2584824A1 (en) * | 2004-10-22 | 2006-05-04 | Vanderbilt University | On-chip polarimetry for high-throughput screening of nanoliter and smaller sample volumes |
| US8548570B2 (en) | 2004-11-29 | 2013-10-01 | Hypermed Imaging, Inc. | Hyperspectral imaging of angiogenesis |
| US8374682B2 (en) | 2005-04-04 | 2013-02-12 | Hypermed Imaging, Inc. | Hyperspectral imaging in diabetes and peripheral vascular disease |
| WO2006058346A1 (en) | 2004-11-29 | 2006-06-01 | The General Hospital Corporation | Arrangements, devices, endoscopes, catheters and methods for performing optical imaging by simultaneously illuminating and detecting multiple points on a sample |
| US8224425B2 (en) * | 2005-04-04 | 2012-07-17 | Hypermed Imaging, Inc. | Hyperspectral imaging in diabetes and peripheral vascular disease |
| CA2631564A1 (en) | 2004-11-29 | 2006-06-01 | Hypermed, Inc. | Medical hyperspectral imaging for evaluation of tissue and tumor |
| CN101916359B (zh) * | 2005-01-27 | 2012-06-20 | 剑桥研究和仪器设备股份有限公司 | 把样本的不同区域分类到相应类别的方法和设备 |
| US20070003996A1 (en) * | 2005-02-09 | 2007-01-04 | Hitt Ben A | Identification of bacteria and spores |
| US8971984B2 (en) * | 2005-04-04 | 2015-03-03 | Hypermed Imaging, Inc. | Hyperspectral technology for assessing and treating diabetic foot and tissue disease |
| US7725169B2 (en) | 2005-04-15 | 2010-05-25 | The Board Of Trustees Of The University Of Illinois | Contrast enhanced spectroscopic optical coherence tomography |
| EP1875436B1 (en) | 2005-04-28 | 2009-12-09 | The General Hospital Corporation | Evaluation of image features of an anatomical structure in optical coherence tomography images |
| US7587078B2 (en) * | 2005-05-02 | 2009-09-08 | Cytyc Corporation | Automated image analysis |
| WO2006124628A2 (en) * | 2005-05-12 | 2006-11-23 | Correlogic Systems, Inc. | A model for classifying a biological sample in relation to breast cancer based on mass spectral data |
| US9060689B2 (en) | 2005-06-01 | 2015-06-23 | The General Hospital Corporation | Apparatus, method and system for performing phase-resolved optical frequency domain imaging |
| ES2354287T3 (es) | 2005-08-09 | 2011-03-11 | The General Hospital Corporation | Aparato y método para realizar una desmodulación en cuadratura por polarización en tomografía de coherencia óptica. |
| US7843572B2 (en) | 2005-09-29 | 2010-11-30 | The General Hospital Corporation | Method and apparatus for optical imaging via spectral encoding |
| US8145018B2 (en) | 2006-01-19 | 2012-03-27 | The General Hospital Corporation | Apparatus for obtaining information for a structure using spectrally-encoded endoscopy techniques and methods for producing one or more optical arrangements |
| EP1973466B1 (en) | 2006-01-19 | 2021-01-06 | The General Hospital Corporation | Ballon imaging catheter |
| WO2007090147A2 (en) * | 2006-01-31 | 2007-08-09 | The Board Of Trustees Of The University Of Illinois | Method and apparatus for measurement of optical properties in tissue |
| JP5524487B2 (ja) | 2006-02-01 | 2014-06-18 | ザ ジェネラル ホスピタル コーポレイション | コンフォーマルレーザ治療手順を用いてサンプルの少なくとも一部分に電磁放射を放射する方法及びシステム。 |
| JP5680829B2 (ja) | 2006-02-01 | 2015-03-04 | ザ ジェネラル ホスピタル コーポレイション | 複数の電磁放射をサンプルに照射する装置 |
| JP2009527770A (ja) | 2006-02-24 | 2009-07-30 | ザ ジェネラル ホスピタル コーポレイション | 角度分解型のフーリエドメイン光干渉断層撮影法を遂行する方法及びシステム |
| US8217943B2 (en) * | 2006-04-21 | 2012-07-10 | Beckman Coulter, Inc. | Displaying cellular analysis result data using a template |
| WO2007133961A2 (en) | 2006-05-10 | 2007-11-22 | The General Hospital Corporation | Processes, arrangements and systems for providing frequency domain imaging of a sample |
| CN101484806A (zh) | 2006-05-17 | 2009-07-15 | 协乐民公司 | 一种对组织进行自动分析的方法 |
| JP5032792B2 (ja) * | 2006-05-22 | 2012-09-26 | 浜松ホトニクス株式会社 | 細胞選別装置 |
| WO2007143141A2 (en) * | 2006-06-01 | 2007-12-13 | The General Hospital Corporation | In-vivo optical imaging method including analysis of dynamic images |
| US8131476B2 (en) * | 2006-08-07 | 2012-03-06 | General Electric Company | System and method for co-registering multi-channel images of a tissue micro array |
| US8060348B2 (en) | 2006-08-07 | 2011-11-15 | General Electric Company | Systems for analyzing tissue samples |
| WO2008039758A2 (en) | 2006-09-25 | 2008-04-03 | Cambridge Research & Instrumentation, Inc. | Sample imaging and classification |
| US8838213B2 (en) | 2006-10-19 | 2014-09-16 | The General Hospital Corporation | Apparatus and method for obtaining and providing imaging information associated with at least one portion of a sample, and effecting such portion(s) |
| JP5558839B2 (ja) | 2007-03-23 | 2014-07-23 | ザ ジェネラル ホスピタル コーポレイション | 角度走査及び分散手順を用いて波長掃引レーザを利用するための方法、構成及び装置 |
| JP5149527B2 (ja) * | 2007-03-29 | 2013-02-20 | 常盤薬品工業株式会社 | 皮膚の色素沈着表示方法 |
| US10534129B2 (en) | 2007-03-30 | 2020-01-14 | The General Hospital Corporation | System and method providing intracoronary laser speckle imaging for the detection of vulnerable plaque |
| WO2008144496A1 (en) * | 2007-05-18 | 2008-11-27 | Vanderbilt University | Improved interferometric detection system and method |
| MY150234A (en) * | 2007-06-29 | 2013-12-31 | Ahn Gook Pharmaceutical Company Ltd | Predictive markers for ovarian cancer |
| US9375158B2 (en) | 2007-07-31 | 2016-06-28 | The General Hospital Corporation | Systems and methods for providing beam scan patterns for high speed doppler optical frequency domain imaging |
| US8265360B2 (en) * | 2007-08-31 | 2012-09-11 | University Of Georgia Research Foundation, Inc. | Methods and systems for analyzing ratiometric data |
| WO2009039466A1 (en) | 2007-09-20 | 2009-03-26 | Vanderbilt University | Free solution measurement of molecular interactions by backscattering interferometry |
| US8330087B2 (en) * | 2007-10-16 | 2012-12-11 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
| US8983580B2 (en) | 2008-01-18 | 2015-03-17 | The Board Of Trustees Of The University Of Illinois | Low-coherence interferometry and optical coherence tomography for image-guided surgical treatment of solid tumors |
| US7751057B2 (en) | 2008-01-18 | 2010-07-06 | The Board Of Trustees Of The University Of Illinois | Magnetomotive optical coherence tomography |
| US8115934B2 (en) | 2008-01-18 | 2012-02-14 | The Board Of Trustees Of The University Of Illinois | Device and method for imaging the ear using optical coherence tomography |
| WO2009111301A1 (en) | 2008-03-04 | 2009-09-11 | 3M Innovative Properties Company | Information management in automated processing of biological growth media |
| BRPI0906086A2 (pt) | 2008-03-04 | 2015-07-07 | 3M Innovative Properties Co | Método, sistemas e meio legível por computador. |
| JP5389380B2 (ja) * | 2008-05-28 | 2014-01-15 | オリンパス株式会社 | 信号処理システム及び信号処理プログラム |
| US8199999B2 (en) * | 2008-06-17 | 2012-06-12 | Cambridge Research & Instrumentation, Inc. | Image classifier training |
| WO2010009136A2 (en) | 2008-07-14 | 2010-01-21 | The General Hospital Corporation | Apparatus and methods for color endoscopy |
| JP4717103B2 (ja) * | 2008-07-18 | 2011-07-06 | オリンパス株式会社 | 信号処理システム及び信号処理プログラム |
| US8644580B2 (en) * | 2008-08-07 | 2014-02-04 | Cambridge Research & Instrumentation, Inc. | Detection of RNA in tissue samples |
| US8280134B2 (en) | 2008-09-22 | 2012-10-02 | Cambridge Research & Instrumentation, Inc. | Multi-spectral imaging including at least one common stain |
| US8937724B2 (en) | 2008-12-10 | 2015-01-20 | The General Hospital Corporation | Systems and methods for extending imaging depth range of optical coherence tomography through optical sub-sampling |
| KR20110106436A (ko) * | 2009-01-09 | 2011-09-28 | 신텔렉트 인코포레이티드 | 세포의 유전적 분석 |
| US8788213B2 (en) | 2009-01-12 | 2014-07-22 | Intrexon Corporation | Laser mediated sectioning and transfer of cell colonies |
| JP2012515576A (ja) | 2009-01-20 | 2012-07-12 | ザ ジェネラル ホスピタル コーポレイション | 内視鏡生検装置、システム、及び方法 |
| CN102308444B (zh) | 2009-02-04 | 2014-06-18 | 通用医疗公司 | 利用高速光学波长调谐源的设备和方法 |
| US20100272651A1 (en) * | 2009-04-22 | 2010-10-28 | Tufts University | Method for assessing potential for tumor development and metastasis |
| BR112012001042A2 (pt) | 2009-07-14 | 2016-11-22 | Gen Hospital Corp | equipamento e método de medição do fluxo de fluído dentro de estrutura anatômica. |
| US9002077B2 (en) * | 2009-08-10 | 2015-04-07 | Cambridge Research & Instrumentation, Inc. | Visualization of stained samples |
| RS61066B1 (sr) | 2010-03-05 | 2020-12-31 | Massachusetts Gen Hospital | Sistemi koji obezbeđuju mikroskopske slike najmanje jedne anatomske strukture na određenoj rezoluciji |
| US8462981B2 (en) | 2010-04-07 | 2013-06-11 | Cambridge Research & Instrumentation, Inc. | Spectral unmixing for visualization of samples |
| US9069130B2 (en) | 2010-05-03 | 2015-06-30 | The General Hospital Corporation | Apparatus, method and system for generating optical radiation from biological gain media |
| US9795301B2 (en) | 2010-05-25 | 2017-10-24 | The General Hospital Corporation | Apparatus, systems, methods and computer-accessible medium for spectral analysis of optical coherence tomography images |
| EP2575597B1 (en) | 2010-05-25 | 2022-05-04 | The General Hospital Corporation | Apparatus for providing optical imaging of structures and compositions |
| US10285568B2 (en) | 2010-06-03 | 2019-05-14 | The General Hospital Corporation | Apparatus and method for devices for imaging structures in or at one or more luminal organs |
| WO2011156713A1 (en) | 2010-06-11 | 2011-12-15 | Vanderbilt University | Multiplexed interferometric detection system and method |
| JP5832537B2 (ja) | 2010-08-05 | 2015-12-16 | ケンブリッジ・リサーチ・アンド・インストルメンテーション・インコーポレーテッド | 試料の強調視覚評価 |
| EP3225990B1 (en) | 2010-08-30 | 2020-07-08 | Konica Minolta, Inc. | Particle for staining a tissue |
| US9976959B2 (en) | 2010-08-31 | 2018-05-22 | Konica Minolta Medical & Graphic, Inc. | Biological substance detection method using florescent dye-containing particle |
| US11378517B2 (en) | 2010-08-31 | 2022-07-05 | Konica Minolta, Inc. | Biological substance detection method |
| JP5384453B2 (ja) | 2010-09-09 | 2014-01-08 | シャープ株式会社 | 測定装置、測定システム、測定方法、制御プログラム、および、記録媒体 |
| EP2632324A4 (en) | 2010-10-27 | 2015-04-22 | Gen Hospital Corp | DEVICES, SYSTEMS AND METHOD FOR MEASURING BLOOD PRESSURE IN AT LEAST ONE VESSEL |
| JP2012098050A (ja) * | 2010-10-29 | 2012-05-24 | Mitaka Koki Co Ltd | モニター可能な分光計測装置 |
| US20130324846A1 (en) * | 2011-02-17 | 2013-12-05 | University Of Massachusetts | Devices and methods for optical pathology |
| US9562853B2 (en) | 2011-02-22 | 2017-02-07 | Vanderbilt University | Nonaqueous backscattering interferometric methods |
| AU2012229102B2 (en) | 2011-03-17 | 2016-02-04 | Cernostics, Inc. | Systems and compositions for diagnosing Barrett's esophagus and methods of using the same |
| US9330092B2 (en) | 2011-07-19 | 2016-05-03 | The General Hospital Corporation | Systems, methods, apparatus and computer-accessible-medium for providing polarization-mode dispersion compensation in optical coherence tomography |
| WO2013035703A1 (ja) | 2011-09-09 | 2013-03-14 | コニカミノルタエムジー株式会社 | 生体物質検出方法 |
| EP2769491A4 (en) | 2011-10-18 | 2015-07-22 | Gen Hospital Corp | DEVICE AND METHOD FOR PRODUCING AND / OR PROVIDING RECIRCULATING OPTICAL DELAY (DE) |
| JP6265119B2 (ja) | 2012-03-30 | 2018-01-24 | コニカミノルタ株式会社 | 生体物質検出方法 |
| WO2013148306A1 (en) | 2012-03-30 | 2013-10-03 | The General Hospital Corporation | Imaging system, method and distal attachment for multidirectional field of view endoscopy |
| US9273949B2 (en) | 2012-05-11 | 2016-03-01 | Vanderbilt University | Backscattering interferometric methods |
| WO2013177154A1 (en) | 2012-05-21 | 2013-11-28 | The General Hospital Corporation | Apparatus, device and method for capsule microscopy |
| WO2013179581A1 (ja) * | 2012-05-30 | 2013-12-05 | パナソニック株式会社 | 画像計測装置、画像計測方法及び画像計測システム |
| EP2888616A4 (en) | 2012-08-22 | 2016-04-27 | Gen Hospital Corp | SYSTEM, METHOD AND COMPUTER-ACCESSIBLE MEDIA FOR MANUFACTURING MINIATURE ENDOSCOPES USING SOFT LITHOGRAPHY |
| WO2014117130A1 (en) | 2013-01-28 | 2014-07-31 | The General Hospital Corporation | Apparatus and method for providing diffuse spectroscopy co-registered with optical frequency domain imaging |
| US10893806B2 (en) | 2013-01-29 | 2021-01-19 | The General Hospital Corporation | Apparatus, systems and methods for providing information regarding the aortic valve |
| US11179028B2 (en) | 2013-02-01 | 2021-11-23 | The General Hospital Corporation | Objective lens arrangement for confocal endomicroscopy |
| JP6378311B2 (ja) | 2013-03-15 | 2018-08-22 | ザ ジェネラル ホスピタル コーポレイション | 物体を特徴付ける方法とシステム |
| EP2997354A4 (en) | 2013-05-13 | 2017-01-18 | The General Hospital Corporation | Detecting self-interefering fluorescence phase and amplitude |
| WO2015009932A1 (en) | 2013-07-19 | 2015-01-22 | The General Hospital Corporation | Imaging apparatus and method which utilizes multidirectional field of view endoscopy |
| US10117576B2 (en) | 2013-07-19 | 2018-11-06 | The General Hospital Corporation | System, method and computer accessible medium for determining eye motion by imaging retina and providing feedback for acquisition of signals from the retina |
| EP3910282B1 (en) | 2013-07-26 | 2024-01-17 | The General Hospital Corporation | Method of providing a laser radiation with a laser arrangement utilizing optical dispersion for applications in fourier-domain optical coherence tomography |
| WO2015105870A1 (en) | 2014-01-08 | 2015-07-16 | The General Hospital Corporation | Method and apparatus for microscopic imaging |
| US10736494B2 (en) | 2014-01-31 | 2020-08-11 | The General Hospital Corporation | System and method for facilitating manual and/or automatic volumetric imaging with real-time tension or force feedback using a tethered imaging device |
| WO2015153982A1 (en) | 2014-04-04 | 2015-10-08 | The General Hospital Corporation | Apparatus and method for controlling propagation and/or transmission of electromagnetic radiation in flexible waveguide(s) |
| KR102513779B1 (ko) | 2014-07-25 | 2023-03-24 | 더 제너럴 하스피탈 코포레이션 | 생체 내 이미징 및 진단을 위한 장치, 디바이스 및 방법 |
| WO2016118812A1 (en) | 2015-01-23 | 2016-07-28 | Vanderbilt University | A robust interferometer and methods of using same |
| CN107209111B (zh) * | 2015-01-31 | 2020-08-28 | 文塔纳医疗系统公司 | 自动化整体载片分析的质量控制 |
| EP3253420A4 (en) * | 2015-02-02 | 2018-10-10 | Novadaq Technologies ULC | Methods and systems for characterizing tissue of a subject |
| GB2542764A (en) * | 2015-09-23 | 2017-04-05 | Pathxl Ltd | Image processing method and apparatus for normalisation and artefact correction |
| US10026159B2 (en) | 2015-09-23 | 2018-07-17 | Novadaq Technologies ULC | Methods and system for management of data derived from medical imaging |
| EP3408649B1 (en) | 2016-01-29 | 2023-06-14 | Vanderbilt University | Free-solution response function interferometry |
| CA3021481A1 (en) | 2016-07-29 | 2018-02-01 | Novadaq Technologies ULC | Methods and systems for characterizing tissue of a subject utilizing machine learning |
| CN106842535B (zh) * | 2017-01-13 | 2019-10-29 | 清华大学 | 基于光流的相位显微成像系统及其方法 |
| CN115791640B (zh) * | 2023-02-06 | 2023-06-02 | 杭州华得森生物技术有限公司 | 基于分光光谱的肿瘤细胞检测设备及其方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4085006A (en) * | 1974-11-06 | 1978-04-18 | Isomedics, Incorporated | Automatic cell analyzer method |
| US4581223A (en) * | 1980-03-12 | 1986-04-08 | Lawrence Kass | Individual leukocyte determination by means of differential metachromatic dye sorption |
| JPS5772914A (en) * | 1980-10-22 | 1982-05-07 | Takeda Chem Ind Ltd | Antitumor agent |
| US4622291A (en) * | 1984-06-11 | 1986-11-11 | The United States Of America As Represented By The Department Of Health And Human Services | Method and device for quantitative end point determination in immunofluorescence using microfluorophotometry |
| US4721669A (en) * | 1985-01-18 | 1988-01-26 | The Trustees Of Columbia University In The City Of New York | Chemical probes for left-handed DNA and chiral metal complexes as Z-specific anti-tumor agents |
| US5194373A (en) * | 1985-06-06 | 1993-03-16 | Thomas Jefferson University | Method of determining endothelial cell coverage of a prosthetic surface |
| US5109429A (en) * | 1985-11-04 | 1992-04-28 | Cell Analysis Systems,Inc. | Apparatus and method for analyses of biological specimens |
| US4998284A (en) * | 1987-11-17 | 1991-03-05 | Cell Analysis Systems, Inc. | Dual color camera microscope and methodology for cell staining and analysis |
| US4859584A (en) * | 1986-10-31 | 1989-08-22 | Smithkline Beckman Corporation | Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes |
| NO884566L (no) * | 1987-10-15 | 1989-04-17 | Univ Moskovsk | Anvendelse av fluorderivater og kontrastpreparat for diagnostisering av ondartede neoplasmer. |
| US5149527A (en) * | 1990-09-18 | 1992-09-22 | Oncotech, Inc. | Immunopotentiating protocol for chemotherapy-responsive tumors |
| US5106744A (en) * | 1990-11-01 | 1992-04-21 | Cytocolor Inc. | Method of staining monocytes and compositions thereof |
| US5817462A (en) * | 1995-02-21 | 1998-10-06 | Applied Spectral Imaging | Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding |
| US5784162A (en) * | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| US5377003A (en) * | 1992-03-06 | 1994-12-27 | The United States Of America As Represented By The Department Of Health And Human Services | Spectroscopic imaging device employing imaging quality spectral filters |
| EP0767361B1 (en) * | 1993-07-22 | 2000-02-23 | Applied Spectral Imaging Ltd. | Method and apparatus for spectral imaging |
| US5732150A (en) * | 1995-09-19 | 1998-03-24 | Ihc Health Services, Inc. | Method and system for multiple wavelength microscopy image analysis |
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| US5995645A (en) | 1999-11-30 |
| IL136190A0 (en) | 2001-05-20 |
| JP2001525580A (ja) | 2001-12-11 |
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