IL130949A - Method for characterising polypeptides - Google Patents
Method for characterising polypeptidesInfo
- Publication number
- IL130949A IL130949A IL13094998A IL13094998A IL130949A IL 130949 A IL130949 A IL 130949A IL 13094998 A IL13094998 A IL 13094998A IL 13094998 A IL13094998 A IL 13094998A IL 130949 A IL130949 A IL 130949A
- Authority
- IL
- Israel
- Prior art keywords
- proteins
- polypeptides
- population
- terminus
- agent
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 171
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 117
- 238000000034 method Methods 0.000 title claims abstract description 97
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 44
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 63
- 230000007017 scission Effects 0.000 claims abstract description 63
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 55
- 102000007079 Peptide Fragments Human genes 0.000 claims abstract description 27
- 108010033276 Peptide Fragments Proteins 0.000 claims abstract description 27
- 239000012634 fragment Substances 0.000 claims abstract description 24
- 125000000539 amino acid group Chemical group 0.000 claims abstract description 13
- 238000004949 mass spectrometry Methods 0.000 claims description 27
- 239000007790 solid phase Substances 0.000 claims description 27
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 24
- 150000001413 amino acids Chemical group 0.000 claims description 20
- 238000004811 liquid chromatography Methods 0.000 claims description 8
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 6
- 102000035195 Peptidases Human genes 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 238000004255 ion exchange chromatography Methods 0.000 claims description 5
- 230000006287 biotinylation Effects 0.000 claims description 4
- 238000007413 biotinylation Methods 0.000 claims description 4
- 239000002981 blocking agent Substances 0.000 claims description 4
- 230000008878 coupling Effects 0.000 claims description 4
- 238000010168 coupling process Methods 0.000 claims description 4
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 3
- 235000019833 protease Nutrition 0.000 claims description 3
- 210000001768 subcellular fraction Anatomy 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 description 176
- 235000018102 proteins Nutrition 0.000 description 173
- 102000004169 proteins and genes Human genes 0.000 description 173
- 239000012528 membrane Substances 0.000 description 39
- 150000002500 ions Chemical class 0.000 description 38
- 238000004458 analytical method Methods 0.000 description 37
- 239000000523 sample Substances 0.000 description 36
- 238000012163 sequencing technique Methods 0.000 description 26
- 108090000631 Trypsin Proteins 0.000 description 23
- 102000004142 Trypsin Human genes 0.000 description 23
- 239000012588 trypsin Substances 0.000 description 23
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 22
- 229940024606 amino acid Drugs 0.000 description 21
- 238000000926 separation method Methods 0.000 description 20
- 238000013467 fragmentation Methods 0.000 description 19
- 238000006062 fragmentation reaction Methods 0.000 description 19
- 239000000243 solution Substances 0.000 description 18
- 239000003153 chemical reaction reagent Substances 0.000 description 17
- 239000011159 matrix material Substances 0.000 description 16
- 239000002033 PVDF binder Substances 0.000 description 14
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 14
- 238000004885 tandem mass spectrometry Methods 0.000 description 14
- 102000016943 Muramidase Human genes 0.000 description 12
- 108010014251 Muramidase Proteins 0.000 description 12
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 12
- 229960002685 biotin Drugs 0.000 description 12
- 239000011616 biotin Substances 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 12
- 235000010335 lysozyme Nutrition 0.000 description 12
- 229960000274 lysozyme Drugs 0.000 description 12
- 239000004325 lysozyme Substances 0.000 description 12
- 239000002904 solvent Substances 0.000 description 12
- 235000020958 biotin Nutrition 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 11
- 238000005194 fractionation Methods 0.000 description 11
- 210000004899 c-terminal region Anatomy 0.000 description 10
- 238000005040 ion trap Methods 0.000 description 9
- 239000007822 coupling agent Substances 0.000 description 8
- 108090001008 Avidin Proteins 0.000 description 7
- 125000003277 amino group Chemical group 0.000 description 7
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- DPBLXKKOBLCELK-UHFFFAOYSA-N pentan-1-amine Chemical compound CCCCCN DPBLXKKOBLCELK-UHFFFAOYSA-N 0.000 description 6
- 230000000717 retained effect Effects 0.000 description 6
- 241000894007 species Species 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 239000013043 chemical agent Substances 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000007789 gas Substances 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 101800001415 Bri23 peptide Proteins 0.000 description 4
- 102400000107 C-terminal peptide Human genes 0.000 description 4
- 101800000655 C-terminal peptide Proteins 0.000 description 4
- 108091000080 Phosphotransferase Proteins 0.000 description 4
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 238000001502 gel electrophoresis Methods 0.000 description 4
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 4
- 239000007791 liquid phase Substances 0.000 description 4
- 238000001819 mass spectrum Methods 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- QKFJKGMPGYROCL-UHFFFAOYSA-N phenyl isothiocyanate Chemical compound S=C=NC1=CC=CC=C1 QKFJKGMPGYROCL-UHFFFAOYSA-N 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- 102000020233 phosphotransferase Human genes 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 230000021736 acetylation Effects 0.000 description 3
- 238000006640 acetylation reaction Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000001412 amines Chemical class 0.000 description 3
- 238000013375 chromatographic separation Methods 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 230000005684 electric field Effects 0.000 description 3
- 230000005686 electrostatic field Effects 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 3
- 229940100684 pentylamine Drugs 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 230000019491 signal transduction Effects 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 2
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 2
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- JGFZNNIVVJXRND-UHFFFAOYSA-N N,N-Diisopropylethylamine (DIPEA) Chemical compound CCN(C(C)C)C(C)C JGFZNNIVVJXRND-UHFFFAOYSA-N 0.000 description 2
- 102400000108 N-terminal peptide Human genes 0.000 description 2
- 101800000597 N-terminal peptide Proteins 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 108010064851 Plant Proteins Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 2
- 239000001099 ammonium carbonate Substances 0.000 description 2
- 239000012805 animal sample Substances 0.000 description 2
- 210000004900 c-terminal fragment Anatomy 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-L glutamate group Chemical group N[C@@H](CCC(=O)[O-])C(=O)[O-] WHUUTDBJXJRKMK-VKHMYHEASA-L 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 229940117953 phenylisothiocyanate Drugs 0.000 description 2
- 230000026731 phosphorylation Effects 0.000 description 2
- 238000006366 phosphorylation reaction Methods 0.000 description 2
- 235000021118 plant-derived protein Nutrition 0.000 description 2
- 150000003141 primary amines Chemical group 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 238000012421 spiking Methods 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- 238000000539 two dimensional gel electrophoresis Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- UGWULZWUXSCWPX-UHFFFAOYSA-N 2-sulfanylideneimidazolidin-4-one Chemical class O=C1CNC(=S)N1 UGWULZWUXSCWPX-UHFFFAOYSA-N 0.000 description 1
- MWOOKDULMBMMPN-UHFFFAOYSA-N 3-(2-ethyl-1,2-oxazol-2-ium-5-yl)benzenesulfonate Chemical compound O1[N+](CC)=CC=C1C1=CC=CC(S([O-])(=O)=O)=C1 MWOOKDULMBMMPN-UHFFFAOYSA-N 0.000 description 1
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical group [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 125000001433 C-terminal amino-acid group Chemical group 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108091060211 Expressed sequence tag Proteins 0.000 description 1
- 238000004252 FT/ICR mass spectrometry Methods 0.000 description 1
- 108010093488 His-His-His-His-His-His Proteins 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108010058682 Mitochondrial Proteins Proteins 0.000 description 1
- 102000006404 Mitochondrial Proteins Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108010022999 Serine Proteases Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- QYGWFFSAYBRMHH-UHFFFAOYSA-N [isothiocyanato(phenoxy)phosphoryl]oxybenzene Chemical compound C=1C=CC=CC=1OP(N=C=S)(=O)OC1=CC=CC=C1 QYGWFFSAYBRMHH-UHFFFAOYSA-N 0.000 description 1
- 230000009435 amidation Effects 0.000 description 1
- 238000007112 amidation reaction Methods 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-L aspartate group Chemical group N[C@@H](CC(=O)[O-])C(=O)[O-] CKLJMWTZIZZHCS-REOHCLBHSA-L 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- -1 benzyl ester Chemical class 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 108091006004 biotinylated proteins Proteins 0.000 description 1
- 229910052792 caesium Inorganic materials 0.000 description 1
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
- 238000000738 capillary electrophoresis-mass spectrometry Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000005277 cation exchange chromatography Methods 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 125000004218 chloromethyl group Chemical group [H]C([H])(Cl)* 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N cinnamic acid group Chemical group C(C=CC1=CC=CC=C1)(=O)O WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000001360 collision-induced dissociation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000000132 electrospray ionisation Methods 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 239000013628 high molecular weight specie Substances 0.000 description 1
- 108091008147 housekeeping proteins Proteins 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000001679 laser desorption electrospray ionisation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 210000004898 n-terminal fragment Anatomy 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010647 peptide synthesis reaction Methods 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- DGTNSSLYPYDJGL-UHFFFAOYSA-N phenyl isocyanate Chemical compound O=C=NC1=CC=CC=C1 DGTNSSLYPYDJGL-UHFFFAOYSA-N 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 125000006239 protecting group Chemical group 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000012070 reactive reagent Substances 0.000 description 1
- 238000006798 ring closing metathesis reaction Methods 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010846 tandem mass spectrometry analysis Methods 0.000 description 1
- 238000011191 terminal modification Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- AAPLIUHOKVUFCC-UHFFFAOYSA-N trimethylsilanol Chemical compound C[Si](C)(C)O AAPLIUHOKVUFCC-UHFFFAOYSA-N 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9701357.7A GB9701357D0 (en) | 1997-01-23 | 1997-01-23 | Characterising polypeptides |
| GBGB9726947.6A GB9726947D0 (en) | 1997-12-19 | 1997-12-19 | Characterising polypeptides |
| PCT/GB1998/000201 WO1998032876A1 (en) | 1997-01-23 | 1998-01-23 | Characterising polypeptides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL130949A0 IL130949A0 (en) | 2001-01-28 |
| IL130949A true IL130949A (en) | 2004-12-15 |
Family
ID=26310845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL13094998A IL130949A (en) | 1997-01-23 | 1998-01-23 | Method for characterising polypeptides |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US6156527A (de) |
| EP (1) | EP1027454B1 (de) |
| JP (1) | JP3795534B2 (de) |
| CN (1) | CN1118580C (de) |
| AT (1) | ATE247173T1 (de) |
| AU (1) | AU721390B2 (de) |
| CA (1) | CA2278556C (de) |
| DE (1) | DE69817211T2 (de) |
| ES (1) | ES2205437T3 (de) |
| IL (1) | IL130949A (de) |
| NZ (1) | NZ336768A (de) |
| WO (1) | WO1998032876A1 (de) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6207370B1 (en) | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
| DE69912444T3 (de) | 1998-08-25 | 2010-05-06 | University Of Washington, Seattle | Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen |
| GB9821393D0 (en) | 1998-10-01 | 1998-11-25 | Brax Genomics Ltd | Protein profiling 2 |
| ATE344456T1 (de) * | 2000-05-05 | 2006-11-15 | Purdue Research Foundation | Durch affinität ausgewählte unterschriftspeptide zur identifizierung und quantifizierung von proteinen |
| JP2004503780A (ja) | 2000-06-12 | 2004-02-05 | ユニバーシティ オブ ワシントン | リンペプチドの選択的標識および単離ならびにプロテオーム分析への適用 |
| WO2002014867A2 (en) * | 2000-08-11 | 2002-02-21 | Agilix Corporation | Ultra-sensitive detection systems |
| US7973936B2 (en) | 2001-01-30 | 2011-07-05 | Board Of Trustees Of Michigan State University | Control system and apparatus for use with ultra-fast laser |
| US7567596B2 (en) | 2001-01-30 | 2009-07-28 | Board Of Trustees Of Michigan State University | Control system and apparatus for use with ultra-fast laser |
| US7450618B2 (en) * | 2001-01-30 | 2008-11-11 | Board Of Trustees Operating Michigan State University | Laser system using ultrashort laser pulses |
| AU2002245345A1 (en) | 2001-01-30 | 2002-08-12 | Board Of Trustees Operating Michigan State University | Control system and apparatus for use with laser excitation or ionization |
| US7609731B2 (en) * | 2001-01-30 | 2009-10-27 | Board Of Trustees Operating Michigan State University | Laser system using ultra-short laser pulses |
| US7583710B2 (en) | 2001-01-30 | 2009-09-01 | Board Of Trustees Operating Michigan State University | Laser and environmental monitoring system |
| US20020106700A1 (en) * | 2001-02-05 | 2002-08-08 | Foote Robert S. | Method for analyzing proteins |
| EP1267170A1 (de) * | 2001-06-07 | 2002-12-18 | Xzillion GmbH & CO.KG | Verfahren zur Charakterisierung von Polypeptiden |
| US7163803B2 (en) * | 2001-06-07 | 2007-01-16 | Electrophoretics Limited | Method for characterizing polypeptides |
| US6800449B1 (en) * | 2001-07-13 | 2004-10-05 | Syngenta Participations Ag | High throughput functional proteomics |
| CA2461587C (en) | 2001-09-27 | 2011-04-26 | Purdue Research Foundation | Materials and methods for controlling isotope effects during fractionation of analytes |
| JP2005514603A (ja) * | 2001-11-06 | 2005-05-19 | アジリックス・コーポレイション | 高感度コード化検出システム |
| AU2003224253A1 (en) | 2002-04-04 | 2003-10-27 | Electrophoretics Limited | Method for characterising analytes |
| US7094345B2 (en) * | 2002-09-09 | 2006-08-22 | Cytonome, Inc. | Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system |
| JP4613299B2 (ja) * | 2003-01-30 | 2011-01-12 | ディーエイチ テクノロジーズ デベロップメント プライベート リミテッド | 分析物分析に関する方法、混合物、キット、および組成物 |
| US20050164324A1 (en) * | 2003-06-04 | 2005-07-28 | Gygi Steven P. | Systems, methods and kits for characterizing phosphoproteomes |
| WO2005042559A2 (en) * | 2003-06-06 | 2005-05-12 | President And Fellows Of Harvard College | Capture and release based isotope tagged peptides and methods for using the same |
| US7579165B2 (en) * | 2003-10-16 | 2009-08-25 | Shimadzu Corporation | Methods for derivatizing protein or peptide with sulfonic acid groups |
| JP4576972B2 (ja) * | 2003-10-16 | 2010-11-10 | 株式会社島津製作所 | タンパク質又はペプチドのスルホン酸誘導体化されたｎ末端ペプチドフラグメントを質量分析する方法 |
| US20050147985A1 (en) * | 2004-01-05 | 2005-07-07 | Applera Corporation | Mixtures of isobarically labeled analytes and fragments ions derived therefrom |
| US7355045B2 (en) * | 2004-01-05 | 2008-04-08 | Applera Corporation | Isotopically enriched N-substituted piperazine acetic acids and methods for the preparation thereof |
| US20050148087A1 (en) * | 2004-01-05 | 2005-07-07 | Applera Corporation | Isobarically labeled analytes and fragment ions derived therefrom |
| US7307169B2 (en) | 2004-01-05 | 2007-12-11 | Applera Corporation | Isotopically enriched N-substituted piperazines and methods for the preparation thereof |
| JP2006078192A (ja) * | 2004-09-07 | 2006-03-23 | Shimadzu Corp | 生体高分子の質量分析法 |
| US20070207555A1 (en) * | 2005-02-03 | 2007-09-06 | Cesar Guerra | Ultra-sensitive detection systems using multidimension signals |
| EP1851532A1 (de) | 2005-02-14 | 2007-11-07 | Board of Trustees of Michigan State University | Ultraschnelles lasersystem |
| GB0515323D0 (en) | 2005-07-26 | 2005-08-31 | Electrophoretics Ltd | Mass labels |
| GB0518585D0 (en) | 2005-09-12 | 2005-10-19 | Electrophoretics Ltd | Mass labels |
| US8618470B2 (en) | 2005-11-30 | 2013-12-31 | Board Of Trustees Of Michigan State University | Laser based identification of molecular characteristics |
| US9018562B2 (en) | 2006-04-10 | 2015-04-28 | Board Of Trustees Of Michigan State University | Laser material processing system |
| US20090002703A1 (en) * | 2006-08-16 | 2009-01-01 | Craig Edward Parman | Methods and systems for quantifying isobaric labels and peptides |
| CN101517416A (zh) * | 2006-09-14 | 2009-08-26 | 皇家飞利浦电子股份有限公司 | 基于鉴定羧基端肽分析蛋白质样品的方法 |
| US8334506B2 (en) | 2007-12-10 | 2012-12-18 | 1St Detect Corporation | End cap voltage control of ion traps |
| US7973277B2 (en) | 2008-05-27 | 2011-07-05 | 1St Detect Corporation | Driving a mass spectrometer ion trap or mass filter |
| US8861075B2 (en) | 2009-03-05 | 2014-10-14 | Board Of Trustees Of Michigan State University | Laser amplification system |
| EP2336783A1 (de) * | 2009-12-18 | 2011-06-22 | Basf Se | C-terminale Sequenzierung von Aminosäuren |
| US8630322B2 (en) | 2010-03-01 | 2014-01-14 | Board Of Trustees Of Michigan State University | Laser system for output manipulation |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| GB2510488B (en) | 2011-06-23 | 2020-04-08 | Univ Texas | Identifying peptides at the single molecule level |
| WO2016069124A1 (en) | 2014-09-15 | 2016-05-06 | Board Of Regents, The University Of Texas System | Improved single molecule peptide sequencing |
| WO2013112745A1 (en) * | 2012-01-24 | 2013-08-01 | The Regents Of The University Of Colorado, A Body Corporate | Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation |
| CN106153712B (zh) * | 2015-04-16 | 2019-02-12 | 深圳华大生命科学研究院 | 一种多肽二硫键的定位方法 |
| AU2019301750A1 (en) | 2018-07-12 | 2021-01-28 | Board Of Regents, The University Of Texas System | Molecular neighborhood detection by oligonucleotides |
| EP3837271A4 (de) * | 2018-08-14 | 2022-06-15 | Board of Regents, The University of Texas System | An den haupthistokompatibilitätskomplex gebundene einzelmolekülsequenzierungspeptide |
| GB2593091B (en) | 2018-10-05 | 2023-12-20 | Univ Texas | Solid-phase N-terminal peptide capture and release |
| CN121148475B (zh) * | 2025-09-15 | 2026-04-07 | 中国科学院生物物理研究所 | aa-score驱动的单一位点多肽聚类策略在肽组学研究中的应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4532207A (en) * | 1982-03-19 | 1985-07-30 | G. D. Searle & Co. | Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase |
| SE8803986D0 (sv) * | 1988-11-03 | 1988-11-03 | Pharmacia Ab | Method for sequencing peptides or proteins |
| JPH0824576B2 (ja) * | 1992-07-22 | 1996-03-13 | 農林水産省食品総合研究所長 | 新規アスパラギニルエンドプロテアーゼとその製造法並びに利用法 |
| US5470703A (en) * | 1992-10-21 | 1995-11-28 | Shimadzu Corporation | Method for peptide C-terminal fragment sequence analysis and apparatus for collecting peptide fragment |
-
1998
- 1998-01-23 DE DE69817211T patent/DE69817211T2/de not_active Expired - Lifetime
- 1998-01-23 ES ES98900949T patent/ES2205437T3/es not_active Expired - Lifetime
- 1998-01-23 US US09/341,993 patent/US6156527A/en not_active Expired - Lifetime
- 1998-01-23 AU AU56745/98A patent/AU721390B2/en not_active Ceased
- 1998-01-23 WO PCT/GB1998/000201 patent/WO1998032876A1/en not_active Ceased
- 1998-01-23 NZ NZ336768A patent/NZ336768A/en unknown
- 1998-01-23 EP EP98900949A patent/EP1027454B1/de not_active Expired - Lifetime
- 1998-01-23 JP JP53172898A patent/JP3795534B2/ja not_active Expired - Fee Related
- 1998-01-23 CN CN98801977.9A patent/CN1118580C/zh not_active Expired - Fee Related
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- 1998-01-23 CA CA002278556A patent/CA2278556C/en not_active Expired - Fee Related
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Also Published As
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|---|---|
| ES2205437T3 (es) | 2004-05-01 |
| EP1027454B1 (de) | 2003-08-13 |
| IL130949A0 (en) | 2001-01-28 |
| AU5674598A (en) | 1998-08-18 |
| ATE247173T1 (de) | 2003-08-15 |
| DE69817211T2 (de) | 2004-06-03 |
| CN1118580C (zh) | 2003-08-20 |
| JP3795534B2 (ja) | 2006-07-12 |
| JP2001508872A (ja) | 2001-07-03 |
| DE69817211D1 (de) | 2003-09-18 |
| AU721390B2 (en) | 2000-06-29 |
| CA2278556A1 (en) | 1998-07-30 |
| US6156527A (en) | 2000-12-05 |
| NZ336768A (en) | 2001-12-21 |
| WO1998032876A1 (en) | 1998-07-30 |
| CA2278556C (en) | 2003-07-29 |
| EP1027454A1 (de) | 2000-08-16 |
| CN1244218A (zh) | 2000-02-09 |
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