IL128212A - Detection of small molecules by use of a piezoelectric sensor - Google Patents
Detection of small molecules by use of a piezoelectric sensorInfo
- Publication number
- IL128212A IL128212A IL12821299A IL12821299A IL128212A IL 128212 A IL128212 A IL 128212A IL 12821299 A IL12821299 A IL 12821299A IL 12821299 A IL12821299 A IL 12821299A IL 128212 A IL128212 A IL 128212A
- Authority
- IL
- Israel
- Prior art keywords
- assayed
- molecule
- sample
- sensing surface
- binding
- Prior art date
Links
- 150000003384 small molecules Chemical class 0.000 title claims description 13
- 238000001514 detection method Methods 0.000 title description 15
- 239000013078 crystal Substances 0.000 claims abstract description 164
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 126
- 230000027455 binding Effects 0.000 claims abstract description 97
- 238000000034 method Methods 0.000 claims abstract description 48
- 230000008859 change Effects 0.000 claims abstract description 47
- 238000002360 preparation method Methods 0.000 claims abstract description 40
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 24
- 230000001939 inductive effect Effects 0.000 claims abstract description 20
- 238000012545 processing Methods 0.000 claims abstract description 8
- 239000000427 antigen Substances 0.000 claims description 81
- 102000036639 antigens Human genes 0.000 claims description 78
- 108091007433 antigens Proteins 0.000 claims description 78
- 108090000623 proteins and genes Proteins 0.000 claims description 54
- 102000004169 proteins and genes Human genes 0.000 claims description 53
- 230000003472 neutralizing effect Effects 0.000 claims description 45
- 230000007423 decrease Effects 0.000 claims description 43
- 238000001914 filtration Methods 0.000 claims description 40
- 239000002360 explosive Substances 0.000 claims description 36
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 30
- 229940088598 enzyme Drugs 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 21
- 108090000790 Enzymes Proteins 0.000 claims description 21
- 239000000706 filtrate Substances 0.000 claims description 20
- 238000012360 testing method Methods 0.000 claims description 19
- 108090001008 Avidin Proteins 0.000 claims description 18
- 229960002685 biotin Drugs 0.000 claims description 15
- 235000020958 biotin Nutrition 0.000 claims description 15
- 239000011616 biotin Substances 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 230000001965 increasing effect Effects 0.000 claims description 12
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 10
- 108010090804 Streptavidin Proteins 0.000 claims description 8
- 241000894007 species Species 0.000 claims description 8
- 239000007795 chemical reaction product Substances 0.000 claims description 7
- 125000000539 amino acid group Chemical group 0.000 claims description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 230000009467 reduction Effects 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 4
- 102000002464 Galactosidases Human genes 0.000 claims description 3
- 108010093031 Galactosidases Proteins 0.000 claims description 3
- 108010015776 Glucose oxidase Proteins 0.000 claims description 3
- 239000004366 Glucose oxidase Substances 0.000 claims description 3
- 230000003197 catalytic effect Effects 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 229940116332 glucose oxidase Drugs 0.000 claims description 3
- 235000019420 glucose oxidase Nutrition 0.000 claims description 3
- 108010029942 microperoxidase Proteins 0.000 claims description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000009149 molecular binding Effects 0.000 claims description 2
- 230000002452 interceptive effect Effects 0.000 claims 2
- 238000004880 explosion Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 description 168
- 239000000243 solution Substances 0.000 description 88
- 210000004027 cell Anatomy 0.000 description 76
- 239000000015 trinitrotoluene Substances 0.000 description 69
- SPSSULHKWOKEEL-UHFFFAOYSA-N 2,4,6-trinitrotoluene Chemical compound CC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O SPSSULHKWOKEEL-UHFFFAOYSA-N 0.000 description 66
- 239000002356 single layer Substances 0.000 description 56
- DYSXLQBUUOPLBB-UHFFFAOYSA-N 2,3-dinitrotoluene Chemical compound CC1=CC=CC([N+]([O-])=O)=C1[N+]([O-])=O DYSXLQBUUOPLBB-UHFFFAOYSA-N 0.000 description 41
- RMBFBMJGBANMMK-UHFFFAOYSA-N 2,4-dinitrotoluene Chemical compound CC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O RMBFBMJGBANMMK-UHFFFAOYSA-N 0.000 description 40
- 238000004458 analytical method Methods 0.000 description 34
- 239000002609 medium Substances 0.000 description 27
- 239000012491 analyte Substances 0.000 description 20
- 238000002347 injection Methods 0.000 description 19
- 239000007924 injection Substances 0.000 description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 18
- 230000003993 interaction Effects 0.000 description 18
- 239000010453 quartz Substances 0.000 description 17
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 17
- 239000011550 stock solution Substances 0.000 description 15
- 239000007853 buffer solution Substances 0.000 description 12
- 238000006073 displacement reaction Methods 0.000 description 12
- 239000000203 mixture Substances 0.000 description 12
- 238000003380 quartz crystal microbalance Methods 0.000 description 12
- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 11
- 238000003556 assay Methods 0.000 description 11
- 238000011534 incubation Methods 0.000 description 10
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 9
- 230000003321 amplification Effects 0.000 description 9
- 238000003199 nucleic acid amplification method Methods 0.000 description 9
- 239000011780 sodium chloride Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 239000012530 fluid Substances 0.000 description 8
- 239000010410 layer Substances 0.000 description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 102100032865 General transcription factor IIH subunit 5 Human genes 0.000 description 7
- 101000655402 Homo sapiens General transcription factor IIH subunit 5 Proteins 0.000 description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 7
- 229940098773 bovine serum albumin Drugs 0.000 description 7
- 229940099500 cystamine Drugs 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- XTRDKALNCIHHNI-UHFFFAOYSA-N 2,6-dinitrotoluene Chemical compound CC1=C([N+]([O-])=O)C=CC=C1[N+]([O-])=O XTRDKALNCIHHNI-UHFFFAOYSA-N 0.000 description 6
- 238000002965 ELISA Methods 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- OOTFVKOQINZBBF-UHFFFAOYSA-N cystamine Chemical compound CCSSCCN OOTFVKOQINZBBF-UHFFFAOYSA-N 0.000 description 6
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 6
- 239000010931 gold Substances 0.000 description 6
- 210000004408 hybridoma Anatomy 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 6
- 239000002184 metal Substances 0.000 description 6
- 239000008363 phosphate buffer Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000036962 time dependent Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- ZYUWUKIAUDIXCQ-UHFFFAOYSA-N N-(2,4-dinitrophenyl)aminohexanoic acid Chemical compound OC(=O)CCCCCNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O ZYUWUKIAUDIXCQ-UHFFFAOYSA-N 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 239000008055 phosphate buffer solution Substances 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- LVSPDZAGCBEQAV-UHFFFAOYSA-N 4-chloronaphthalen-1-ol Chemical compound C1=CC=C2C(O)=CC=C(Cl)C2=C1 LVSPDZAGCBEQAV-UHFFFAOYSA-N 0.000 description 4
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 239000013615 primer Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 206010003445 Ascites Diseases 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- RPENMORRBUTCPR-UHFFFAOYSA-M sodium;1-hydroxy-2,5-dioxopyrrolidine-3-sulfonate Chemical compound [Na+].ON1C(=O)CC(S([O-])(=O)=O)C1=O RPENMORRBUTCPR-UHFFFAOYSA-M 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 108010042685 trinitrophenyl keyhole limpet hemocyanin Proteins 0.000 description 3
- 108010055746 trinitrophenyl-bovine serum albumin Proteins 0.000 description 3
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 2
- XNWFRZJHXBZDAG-UHFFFAOYSA-N 2-METHOXYETHANOL Chemical compound COCCO XNWFRZJHXBZDAG-UHFFFAOYSA-N 0.000 description 2
- INYDMNPNDHRJQJ-UHFFFAOYSA-N 3,4-dinitrotoluene Chemical compound CC1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1 INYDMNPNDHRJQJ-UHFFFAOYSA-N 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000237988 Patellidae Species 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 229910052786 argon Inorganic materials 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000002163 immunogen Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- RZXMPPFPUUCRFN-UHFFFAOYSA-N p-toluidine Chemical compound CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002494 quartz crystal microgravimetry Methods 0.000 description 2
- 239000012088 reference solution Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 210000004989 spleen cell Anatomy 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 2
- BPSIOYPQMFLKFR-UHFFFAOYSA-N trimethoxy-[3-(oxiran-2-ylmethoxy)propyl]silane Chemical group CO[Si](OC)(OC)CCCOCC1CO1 BPSIOYPQMFLKFR-UHFFFAOYSA-N 0.000 description 2
- UVGHPGOONBRLCX-NJSLBKSFSA-N (2,5-dioxopyrrolidin-1-yl) 6-[5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]hexanoate Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)NCCCCCC(=O)ON1C(=O)CCC1=O UVGHPGOONBRLCX-NJSLBKSFSA-N 0.000 description 1
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Substances CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 1
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- NHJVRSWLHSJWIN-UHFFFAOYSA-N 2,4,6-trinitrobenzenesulfonic acid Chemical compound OS(=O)(=O)C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O NHJVRSWLHSJWIN-UHFFFAOYSA-N 0.000 description 1
- 125000001894 2,4,6-trinitrophenyl group Chemical group [H]C1=C(C(*)=C(C([H])=C1[N+]([O-])=O)[N+]([O-])=O)[N+]([O-])=O 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- VCESGVLABVSDRO-UHFFFAOYSA-L 2-[4-[4-[3,5-bis(4-nitrophenyl)tetrazol-2-ium-2-yl]-3-methoxyphenyl]-2-methoxyphenyl]-3,5-bis(4-nitrophenyl)tetrazol-2-ium;dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC(=CC=2)[N+]([O-])=O)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC(=CC=2)[N+]([O-])=O)=NN1C1=CC=C([N+]([O-])=O)C=C1 VCESGVLABVSDRO-UHFFFAOYSA-L 0.000 description 1
- PZEUTLIKVUEDLB-UHFFFAOYSA-N 2-[[[2-[[6-amino-2-[[2-[[6-amino-2-[[2-[[2-[[2-[[2-[[2-[2-[[1-[2-[[2-[[2-[[2-[[2-[[2-[[6-amino-2-[[2-[[2-[2-[[2-[[2-[(2-aminoacetyl)amino]-3-methylpentanoyl]amino]acetyl]amino]propanoylamino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-methylbutanoyl]amino]acetyl]amino]-4-methylpentanoyl]pyrrolidine-2-carbonyl]amino]propanoylamino]-4-methylpentanoyl]amino]-3-methylpentanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-3-methylpentanoyl]amino]hexanoyl]amino]-5-carbamimidamidopentanoyl]amino]hexanoyl]amino]-3-(carbamoylamino)propanoyl]-(3-amino-3-oxopropyl)carbamoyl]amino]pentanediamide Chemical compound CCC(C)C(NC(=O)CN)C(=O)NCC(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NC(CCCCN)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)O)C(=O)NC(C(C)C)C(=O)NCC(=O)NC(CC(C)C)C(=O)N1CCCC1C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(C(C)CC)C(=O)NC(CO)C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)NC(C(C)CC)C(=O)NC(CCCCN)C(=O)NC(CCCNC(N)=N)C(=O)NC(CCCCN)C(=O)NC(CNC(N)=O)C(=O)N(CCC(N)=O)C(=O)NC(CCC(N)=O)C(N)=O PZEUTLIKVUEDLB-UHFFFAOYSA-N 0.000 description 1
- WDMUXYQIMRDWRC-UHFFFAOYSA-N 2-hydroxy-3,4-dinitrobenzoic acid Chemical compound OC(=O)C1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1O WDMUXYQIMRDWRC-UHFFFAOYSA-N 0.000 description 1
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 1
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 1
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
- KJDSORYAHBAGPP-UHFFFAOYSA-N 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydron;tetrachloride Chemical compound Cl.Cl.Cl.Cl.C1=C(N)C(N)=CC=C1C1=CC=C(N)C(N)=C1 KJDSORYAHBAGPP-UHFFFAOYSA-N 0.000 description 1
- FMIDYXXMYUVLMC-UHFFFAOYSA-N 6-hydroxy-3,6-dinitrocyclohexa-2,4-diene-1-carboxylic acid Chemical compound OC(=O)C1C=C([N+]([O-])=O)C=CC1(O)[N+]([O-])=O FMIDYXXMYUVLMC-UHFFFAOYSA-N 0.000 description 1
- RMHZMJVUTKSVKC-UHFFFAOYSA-N 6-hydroxy-4,6-dinitrocyclohexa-2,4-diene-1-carboxylic acid Chemical compound OC(=O)C1C=CC([N+]([O-])=O)=CC1(O)[N+]([O-])=O RMHZMJVUTKSVKC-UHFFFAOYSA-N 0.000 description 1
- OXEUETBFKVCRNP-UHFFFAOYSA-N 9-ethyl-3-carbazolamine Chemical compound NC1=CC=C2N(CC)C3=CC=CC=C3C2=C1 OXEUETBFKVCRNP-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- BIZULEWPNGZPNN-WBGPXRNDSA-N C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)(=O)NC(C(=O)O)CCCC Chemical compound C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)(=O)NC(C(=O)O)CCCC BIZULEWPNGZPNN-WBGPXRNDSA-N 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- GVGLGOZIDCSQPN-PVHGPHFFSA-N Heroin Chemical compound O([C@H]1[C@H](C=C[C@H]23)OC(C)=O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4OC(C)=O GVGLGOZIDCSQPN-PVHGPHFFSA-N 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- AFBPFSWMIHJQDM-UHFFFAOYSA-N N-methyl-N-phenylamine Natural products CNC1=CC=CC=C1 AFBPFSWMIHJQDM-UHFFFAOYSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 229920002292 Nylon 6 Polymers 0.000 description 1
- 241000209094 Oryza Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 238000012443 analytical study Methods 0.000 description 1
- 230000001032 anti-candidal effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000003659 bee venom Substances 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229960003920 cocaine Drugs 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 229960002069 diamorphine Drugs 0.000 description 1
- 238000011549 displacement method Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000000548 hind-foot Anatomy 0.000 description 1
- 229940066369 honey bee venom Drugs 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 210000004201 immune sera Anatomy 0.000 description 1
- 229940042743 immune sera Drugs 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- NGDIAZZSCVVCEW-UHFFFAOYSA-M sodium;butyl sulfate Chemical compound [Na+].CCCCOS([O-])(=O)=O NGDIAZZSCVVCEW-UHFFFAOYSA-M 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229940034208 thyroxine Drugs 0.000 description 1
- 238000001771 vacuum deposition Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Peptides Or Proteins (AREA)
- Measurement Of Length, Angles, Or The Like Using Electric Or Magnetic Means (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Piezo-Electric Transducers For Audible Bands (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Fluid Pressure (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL12821299A IL128212A (en) | 1999-01-25 | 1999-01-25 | Detection of small molecules by use of a piezoelectric sensor |
PCT/IL2000/000048 WO2000043774A2 (fr) | 1999-01-25 | 2000-01-25 | Detection de petites molecules a l'aise d'un capteur piezo-electrique |
US09/889,936 US7135295B1 (en) | 1999-01-25 | 2000-01-25 | Detection of small molecules by use of a piezoelectric sensor |
DE60038363T DE60038363T2 (de) | 1999-01-25 | 2000-01-25 | Detektion von kleinen molekülen mit piezo-elektrischem sensor |
AU21264/00A AU2126400A (en) | 1999-01-25 | 2000-01-25 | Detection of small molecules by use of a piezoelectric sensor |
EP00901308A EP1145006B1 (fr) | 1999-01-25 | 2000-01-25 | Detection de petites molecules a l'aise d'un capteur piezo-electrique |
AT00901308T ATE389881T1 (de) | 1999-01-25 | 2000-01-25 | Detektion von kleinen molekülen mit piezo- elektrischem sensor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL12821299A IL128212A (en) | 1999-01-25 | 1999-01-25 | Detection of small molecules by use of a piezoelectric sensor |
Publications (2)
Publication Number | Publication Date |
---|---|
IL128212A0 IL128212A0 (en) | 1999-11-30 |
IL128212A true IL128212A (en) | 2004-06-20 |
Family
ID=11072413
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL12821299A IL128212A (en) | 1999-01-25 | 1999-01-25 | Detection of small molecules by use of a piezoelectric sensor |
Country Status (7)
Country | Link |
---|---|
US (1) | US7135295B1 (fr) |
EP (1) | EP1145006B1 (fr) |
AT (1) | ATE389881T1 (fr) |
AU (1) | AU2126400A (fr) |
DE (1) | DE60038363T2 (fr) |
IL (1) | IL128212A (fr) |
WO (1) | WO2000043774A2 (fr) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2490210A1 (fr) | 2002-06-19 | 2003-12-31 | Biosensor Applications Sweden Ab (Publ) | Surface metallique revetue situee sur un support solide et utile dans la detection d'analyte par deplacement |
EP1514111A1 (fr) * | 2002-06-19 | 2005-03-16 | Biosensor Applications Sweden AB (Publ) | Surface de metal revetu sur un support solide pour effectuer des reactions de deplacement |
FI20030615A0 (fi) * | 2003-04-24 | 2003-04-24 | Marko Virta | Nanopartikkeli bioaffiniteettimäärityksiin |
JP2007512528A (ja) * | 2003-11-20 | 2007-05-17 | バイオセンサー、アプリケーションズ、スエーデン、アクチボラグ | 所定の抗原混合物に特異的な少なくとも2種類の異なる抗体の混合物およびその混合物の使用 |
US20080100279A1 (en) * | 2006-06-15 | 2008-05-01 | University Of South Florida | Nano-Based Device for Detection of Disease Biomarkers and Other Target Molecules |
US8349604B2 (en) * | 2006-06-15 | 2013-01-08 | University Of South Florida | Nano-based device for detection of disease biomarkers and other target molecules |
CN101595387B (zh) * | 2006-12-13 | 2014-01-29 | 生物传感器应用国际有限公司 | 在测试体积中测试抗原的连续可重复方法 |
US20100112720A1 (en) * | 2006-12-13 | 2010-05-06 | Biosensor Applications Sweden Ab | Continuously repeatable method of detecting antigens in test volume |
US8409875B2 (en) | 2010-10-20 | 2013-04-02 | Rapid Diagnostek, Inc. | Measurement of binding kinetics with a resonating sensor |
US8771402B2 (en) * | 2011-06-14 | 2014-07-08 | Ut-Battelle, Llc | Membrane based apparatus for measurement of volatile particles |
AU2012300227B2 (en) * | 2011-08-26 | 2016-03-10 | Aviana Molecular Technologies, Llc | Biocoated piezoelectric biosensor platform for point-of-care diagnostic use |
PL3194962T3 (pl) | 2014-09-15 | 2019-09-30 | Qorvo Us, Inc. | Detekcja masy poprzez sprzęganie redoks |
CN104407150B (zh) * | 2014-11-12 | 2016-03-30 | 浙江大学 | 用于2,4,6-tnt检测的生物纳米传感器制备方法 |
FR3032792B1 (fr) * | 2015-02-13 | 2017-03-24 | Inst De Rech Pour Le Dev (Ird) | Dispositif de captage d'au moins une espece chimique comprenant un capteur chimique et procede de fabrication d'un tel capteur chimique |
EP3478718A4 (fr) | 2016-07-01 | 2020-07-29 | Paracrine Therapeutics AB | Procédés et compositions pour l'inhibition du pdgf-cc |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4242096A (en) | 1977-11-14 | 1980-12-30 | Minnesota Mining And Manufacturing Company | Immunoassay for antigens |
US4314821A (en) * | 1979-04-09 | 1982-02-09 | Minnesota Mining And Manufacturing Company | Sandwich immunoassay using piezoelectric oscillator |
US4236893A (en) | 1979-04-09 | 1980-12-02 | Minnesota Mining And Manufacturing Company | Method for the assay of classes of antigen-specific antibodies |
US4735906A (en) | 1984-11-28 | 1988-04-05 | Texas A&M University | Sensor having piezoelectric crystal for microgravimetric immunoassays |
US4999284A (en) | 1988-04-06 | 1991-03-12 | E. I. Du Pont De Nemours And Company | Enzymatically amplified piezoelectric specific binding assay |
JPH01269485A (ja) * | 1988-04-20 | 1989-10-26 | Matsushita Electric Ind Co Ltd | モノクローナル抗体産生細胞ラインおよびモノクローナル抗体 |
JPH01269488A (ja) * | 1988-04-20 | 1989-10-26 | Matsushita Electric Ind Co Ltd | モノクローナル抗体産生細胞ラインおよびモノクローナル抗体 |
EP0494896B1 (fr) | 1989-10-04 | 1994-07-06 | E.I. Du Pont De Nemours And Company | Methode d'analyse pour des complexes de cibles biologiques situes a la surface d'un capteur biologique |
US5179028A (en) | 1990-04-20 | 1993-01-12 | Hughes Aircraft Company | Antibody coated crystal chemical sensor |
IL114692A (en) | 1995-07-21 | 1999-10-28 | Yissum Res Dev Co | Determination of an analyte in a liquid medium |
JP3992114B2 (ja) | 1997-06-12 | 2007-10-17 | バイオセンサー、アプリケーションズ、スエーデン、アクチボラグ | 空気中の検体の検出のための装置、システムおよび方法 |
-
1999
- 1999-01-25 IL IL12821299A patent/IL128212A/en not_active IP Right Cessation
-
2000
- 2000-01-25 AU AU21264/00A patent/AU2126400A/en not_active Abandoned
- 2000-01-25 EP EP00901308A patent/EP1145006B1/fr not_active Expired - Lifetime
- 2000-01-25 WO PCT/IL2000/000048 patent/WO2000043774A2/fr active IP Right Grant
- 2000-01-25 US US09/889,936 patent/US7135295B1/en not_active Expired - Fee Related
- 2000-01-25 DE DE60038363T patent/DE60038363T2/de not_active Expired - Lifetime
- 2000-01-25 AT AT00901308T patent/ATE389881T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
IL128212A0 (en) | 1999-11-30 |
EP1145006B1 (fr) | 2008-03-19 |
WO2000043774A3 (fr) | 2001-03-01 |
DE60038363T2 (de) | 2009-04-23 |
WO2000043774A2 (fr) | 2000-07-27 |
ATE389881T1 (de) | 2008-04-15 |
AU2126400A (en) | 2000-08-07 |
EP1145006A2 (fr) | 2001-10-17 |
DE60038363D1 (de) | 2008-04-30 |
US7135295B1 (en) | 2006-11-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Suleiman et al. | Recent developments in piezoelectric immunosensors. A review | |
Marquette et al. | State of the art and recent advances in immunoanalytical systems | |
US7135295B1 (en) | Detection of small molecules by use of a piezoelectric sensor | |
JP2803877B2 (ja) | 巨大分子の特性決定法 | |
US5492840A (en) | Surface plasmon resonance sensor unit and its use in biosensor systems | |
EP0843816B1 (fr) | Determination d'un analyte dans un milieu liquide | |
González-Martı́nez et al. | On-line immunoanalysis for environmental pollutants: from batch assays to automated sensors | |
US7329536B2 (en) | Piezoimmunosensor | |
SE501013C2 (sv) | Förfarande för förbättring av analys med bindning till fast fas | |
Shen et al. | Engineered recombinant single-chain fragment variable antibody for immunosensors | |
KR20090034999A (ko) | 응집 저해 측정법 및 응집 저해 측정용 시약 | |
US20080050795A1 (en) | Mixture Of At Least Two Different Antibodies Specific For Predetermined Antigens And Use Of The Mixture | |
Sakai et al. | Selective and repeatable detection of human serum albumin by using piezoelectric immunosensor | |
Skládal et al. | Kinetic studies of affinity interactions: comparison of piezoelectric and resonant mirror-based biosensors | |
US6410251B2 (en) | Method for detecting or assaying target substance by utilizing oxygen electrode | |
JP4663318B2 (ja) | 置換による分析物検出に有用な固体担体上の被覆された金属表面 | |
EP3427054A2 (fr) | Étalons internes pour dosages immunologiques et leur utilisation | |
CA2309599C (fr) | Ameliorations apportees aux analyses de deplacement | |
CN102301229A (zh) | 在液体样本中的几种靶抗原的分析 | |
WO1998040739A1 (fr) | Biocapteur pour cellules | |
Kim et al. | Direct-binding quartz crystal microbalance immunosensor to detect carp metallothionein | |
Hübner | Development of Immunological Methods for the Detection of Micropollutants in Fresh Water Samples | |
Mascini et al. | Immunosensors based on piezoelectric crystal device | |
Carroll | Development of bead injection methodology for immunoassays | |
JPH09292397A (ja) | バイオセンサ、キット、生体物質の分析方法及びバイオセンサの製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FF | Patent granted | ||
KB | Patent renewed | ||
KB | Patent renewed | ||
KB | Patent renewed | ||
MM9K | Patent not in force due to non-payment of renewal fees |