IL109382A - In vitro method for inserting a heterologous gene - Google Patents
In vitro method for inserting a heterologous geneInfo
- Publication number
- IL109382A IL109382A IL10938294A IL10938294A IL109382A IL 109382 A IL109382 A IL 109382A IL 10938294 A IL10938294 A IL 10938294A IL 10938294 A IL10938294 A IL 10938294A IL 109382 A IL109382 A IL 109382A
- Authority
- IL
- Israel
- Prior art keywords
- heterologous gene
- vitro method
- contg
- host genome
- descendants
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/60—Vectors containing traps for, e.g. exons, promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Lubricants (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB939308271A GB9308271D0 (en) | 1993-04-21 | 1993-04-21 | Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method |
GB939313323A GB9313323D0 (en) | 1993-06-28 | 1993-06-28 | Expression of heterologous genes |
GB9401011A GB9401011D0 (en) | 1994-01-20 | 1994-01-20 | Expression of heterologous genes |
Publications (2)
Publication Number | Publication Date |
---|---|
IL109382A0 IL109382A0 (en) | 1994-07-31 |
IL109382A true IL109382A (en) | 2005-08-31 |
Family
ID=27266666
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL10938294A IL109382A (en) | 1993-04-21 | 1994-04-21 | In vitro method for inserting a heterologous gene |
Country Status (12)
Country | Link |
---|---|
US (3) | US6150169A (ja) |
EP (2) | EP0695361B2 (ja) |
JP (3) | JP4375759B2 (ja) |
AT (1) | ATE247171T1 (ja) |
AU (1) | AU678234B2 (ja) |
CA (1) | CA2161088A1 (ja) |
DE (1) | DE69433034T3 (ja) |
DK (1) | DK0695361T4 (ja) |
IL (1) | IL109382A (ja) |
NZ (1) | NZ265091A (ja) |
SG (1) | SG48698A1 (ja) |
WO (1) | WO1994024301A1 (ja) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994024301A1 (en) | 1993-04-21 | 1994-10-27 | The University Of Edinburgh | Expression of heterologous genes according to a targeted expression profile |
DE19514310A1 (de) * | 1995-04-18 | 1996-10-24 | Univ Ludwigs Albert | Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen |
GB9524973D0 (en) | 1995-12-06 | 1996-02-07 | Lynxvale Ltd | Viral vectors |
US5928888A (en) * | 1996-09-26 | 1999-07-27 | Aurora Biosciences Corporation | Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities |
US7332338B2 (en) | 1996-10-04 | 2008-02-19 | Lexicon Pharmaceuticals, Inc. | Vectors for making genomic modifications |
AU736267B2 (en) * | 1996-12-31 | 2001-07-26 | Omeros Corporation | Vectors and methods for the mutagenesis of mammalian genes |
US6245564B1 (en) | 1997-01-23 | 2001-06-12 | Cornell Research Foundation, Inc. | Method for separating cells |
GB2331752B (en) * | 1997-11-28 | 2001-01-24 | Medical Res Council | Vectors |
US6808921B1 (en) | 1998-03-27 | 2004-10-26 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
US6436707B1 (en) * | 1998-03-27 | 2002-08-20 | Lexicon Genetics Incorporated | Vectors for gene mutagenesis and gene discovery |
CA2325560A1 (en) * | 1998-04-14 | 1999-10-21 | Chiron Corporation | Noncloning technique for expressing a gene of interest |
GB9819912D0 (en) | 1998-09-11 | 1998-11-04 | Univ Edinburgh | Propagation and/or derivation of embryonic stem cells |
US7531715B1 (en) | 1999-01-13 | 2009-05-12 | Ppl Therapeutics (Scotland) | Double nuclear transfer method and results thereof |
US20050060101A1 (en) * | 1999-06-28 | 2005-03-17 | Bevilacqua Michael P. | Systems and methods for characterizing a biological condition or agent using precision gene expression profiles |
US20040225449A1 (en) * | 1999-06-28 | 2004-11-11 | Bevilacqua Michael P. | Systems and methods for characterizing a biological condition or agent using selected gene expression profiles |
US6960439B2 (en) * | 1999-06-28 | 2005-11-01 | Source Precision Medicine, Inc. | Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles |
US20060115826A1 (en) * | 1999-06-28 | 2006-06-01 | Michael Bevilacqua | Gene expression profiling for identification monitoring and treatment of multiple sclerosis |
US6692916B2 (en) * | 1999-06-28 | 2004-02-17 | Source Precision Medicine, Inc. | Systems and methods for characterizing a biological condition or agent using precision gene expression profiles |
AU2002242854A1 (en) | 2001-03-21 | 2002-10-03 | Geron Corporation | Use of telomerase reverse transcriptase to create homozygous knockout animals |
CA2449303C (en) * | 2001-06-06 | 2014-10-14 | Regeneron Pharmaceuticals, Inc. | Method for targeting transcriptionally active loci |
US7473557B2 (en) * | 2001-06-06 | 2009-01-06 | Regeneron Pharmaceuticals, Inc. | Method for targeting transcriptionally active loci |
WO2003040404A1 (en) | 2001-11-09 | 2003-05-15 | Source Precision Medicine, Inc. | Identification, monitoring and treatment of disease and characterization of biological condition using gene expression profiles |
EP1994824A1 (en) * | 2001-11-15 | 2008-11-26 | Kirin Pharma Kabushiki Kaisha | Chimeric non-human animal |
US20030157641A1 (en) * | 2001-11-16 | 2003-08-21 | Idec Pharmaceuticals Corporation | Polycistronic expression of antibodies |
WO2007136865A2 (en) * | 2006-05-19 | 2007-11-29 | Glycofi, Inc | Recombinant vectors |
CN101454447A (zh) | 2006-06-01 | 2009-06-10 | 教会高等教育科研与病人护理协会 | 与细胞pH相关的植物核酸及其用途 |
US20110198764A1 (en) * | 2010-02-18 | 2011-08-18 | Tekni-Plex, Inc. | Aeration system with antimicrobial properties |
EP2383339A1 (en) * | 2010-04-27 | 2011-11-02 | Harald Von Melchner | Gene trapping for use in gene therapy |
WO2013089123A1 (ja) * | 2011-12-13 | 2013-06-20 | 公立大学法人横浜市立大学 | 遺伝子ターゲティングベクター及びその利用方法 |
ES2828663T3 (es) * | 2012-04-18 | 2021-05-27 | Univ Leland Stanford Junior | Dirección genética no disruptiva |
MX2016011690A (es) | 2014-03-21 | 2016-11-07 | Univ Leland Stanford Junior | Edicion del genoma sin nucleasas. |
EP4219709A1 (en) | 2016-11-03 | 2023-08-02 | Temple University - Of The Commonwealth System of Higher Education | Dna plasmids for the fast generation of homologous recombination vectors for cell line development |
WO2020081438A1 (en) | 2018-10-16 | 2020-04-23 | Blueallele, Llc | Methods for targeted insertion of dna in genes |
BR112023012422A2 (pt) | 2021-01-24 | 2023-12-12 | Michael David Forrest | Inibidores da atp sintase - usos cosmético e terapêutico |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2646438B1 (fr) * | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
DE69032809T2 (de) * | 1989-11-06 | 1999-07-08 | Cell Genesys, Inc., Foster City, Calif. | Herstellung von Proteinen mittels homologer Rekombination |
AU664847B2 (en) * | 1991-05-15 | 1995-12-07 | Cell Genesys, Inc. | Genomic modifications with homologous DNA targeting |
WO1993007266A1 (en) * | 1991-10-07 | 1993-04-15 | Idaho Research Foundation, Inc. | Genetic construct for selection of homologous recombinants on a single selective medium |
PT101031B (pt) * | 1991-11-05 | 2002-07-31 | Transkaryotic Therapies Inc | Processo para o fornecimento de proteinas por terapia genetica |
WO1994024301A1 (en) | 1993-04-21 | 1994-10-27 | The University Of Edinburgh | Expression of heterologous genes according to a targeted expression profile |
US6248934B1 (en) * | 1998-10-26 | 2001-06-19 | The Regents Of The University Of California | Gene trap vectors |
-
1994
- 1994-04-21 WO PCT/GB1994/000849 patent/WO1994024301A1/en active Search and Examination
- 1994-04-21 AT AT94913175T patent/ATE247171T1/de not_active IP Right Cessation
- 1994-04-21 JP JP52294494A patent/JP4375759B2/ja not_active Expired - Lifetime
- 1994-04-21 IL IL10938294A patent/IL109382A/xx not_active IP Right Cessation
- 1994-04-21 DE DE69433034T patent/DE69433034T3/de not_active Expired - Lifetime
- 1994-04-21 EP EP94913175A patent/EP0695361B2/en not_active Expired - Lifetime
- 1994-04-21 EP EP03018296A patent/EP1403376A3/en not_active Withdrawn
- 1994-04-21 CA CA002161088A patent/CA2161088A1/en not_active Abandoned
- 1994-04-21 US US08/537,765 patent/US6150169A/en not_active Expired - Lifetime
- 1994-04-21 SG SG1995001828A patent/SG48698A1/en unknown
- 1994-04-21 NZ NZ265091A patent/NZ265091A/en not_active IP Right Cessation
- 1994-04-21 DK DK94913175.9T patent/DK0695361T4/da active
- 1994-04-21 AU AU65427/94A patent/AU678234B2/en not_active Ceased
-
1999
- 1999-07-07 US US09/348,469 patent/US7005299B1/en not_active Expired - Fee Related
-
2006
- 2006-02-28 US US11/364,042 patent/US20090241207A1/en not_active Abandoned
-
2007
- 2007-08-29 JP JP2007222831A patent/JP2007306942A/ja active Pending
-
2008
- 2008-09-26 JP JP2008247444A patent/JP2009017889A/ja not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
DK0695361T3 (da) | 2003-10-27 |
ATE247171T1 (de) | 2003-08-15 |
US20090241207A1 (en) | 2009-09-24 |
NZ265091A (en) | 1997-07-27 |
DE69433034T2 (de) | 2004-06-09 |
DK0695361T4 (da) | 2010-03-22 |
JP2007306942A (ja) | 2007-11-29 |
IL109382A0 (en) | 1994-07-31 |
EP0695361A1 (en) | 1996-02-07 |
US7005299B1 (en) | 2006-02-28 |
EP1403376A3 (en) | 2007-08-29 |
JP4375759B2 (ja) | 2009-12-02 |
JP2009017889A (ja) | 2009-01-29 |
AU678234B2 (en) | 1997-05-22 |
WO1994024301A1 (en) | 1994-10-27 |
DE69433034T3 (de) | 2010-04-15 |
SG48698A1 (en) | 1998-05-18 |
CA2161088A1 (en) | 1994-10-27 |
DE69433034D1 (de) | 2003-09-18 |
EP0695361B1 (en) | 2003-08-13 |
EP1403376A2 (en) | 2004-03-31 |
AU6542794A (en) | 1994-11-08 |
US6150169A (en) | 2000-11-21 |
EP0695361B2 (en) | 2009-11-11 |
JPH09500005A (ja) | 1997-01-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FF | Patent granted | ||
KB20 | Patent renewed for 20 years | ||
EXP | Patent expired |