IE38174B1 - Process for purifying an enzyme - Google Patents
Process for purifying an enzymeInfo
- Publication number
- IE38174B1 IE38174B1 IE100473A IE100473A IE38174B1 IE 38174 B1 IE38174 B1 IE 38174B1 IE 100473 A IE100473 A IE 100473A IE 100473 A IE100473 A IE 100473A IE 38174 B1 IE38174 B1 IE 38174B1
- Authority
- IE
- Ireland
- Prior art keywords
- enzyme
- surface active
- cells
- precipitate
- escherichia coli
- Prior art date
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 5
- 102000004190 Enzymes Human genes 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 abstract 4
- 239000004094 surface-active agent Substances 0.000 abstract 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 125000002091 cationic group Chemical group 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 102000002464 Galactosidases Human genes 0.000 abstract 1
- 108010093031 Galactosidases Proteins 0.000 abstract 1
- 102000011931 Nucleoproteins Human genes 0.000 abstract 1
- 108010061100 Nucleoproteins Proteins 0.000 abstract 1
- 108010073038 Penicillin Amidase Proteins 0.000 abstract 1
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 235000019270 ammonium chloride Nutrition 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 239000001166 ammonium sulphate Substances 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/84—Penicillin amidase (3.5.1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2673372 | 1972-07-07 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE38174L IE38174L (en) | 1974-01-07 |
| IE38174B1 true IE38174B1 (en) | 1978-01-18 |
Family
ID=11220129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE100473A IE38174B1 (en) | 1972-07-07 | 1973-06-18 | Process for purifying an enzyme |
Country Status (9)
| Country | Link |
|---|---|
| BE (1) | BE801351A (enrdf_load_html_response) |
| DE (2) | DE2366417C (enrdf_load_html_response) |
| DK (1) | DK133952B (enrdf_load_html_response) |
| FR (1) | FR2192114B1 (enrdf_load_html_response) |
| GB (1) | GB1411503A (enrdf_load_html_response) |
| IE (1) | IE38174B1 (enrdf_load_html_response) |
| LU (1) | LU67902A1 (enrdf_load_html_response) |
| NL (1) | NL7309439A (enrdf_load_html_response) |
| NO (1) | NO138451C (enrdf_load_html_response) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4055469A (en) * | 1976-12-10 | 1977-10-25 | Eastman Kodak Company | Purification of microbial enzyme extracts using synthetic polyelectrolytes |
| JPS56109586A (en) * | 1980-02-05 | 1981-08-31 | Cpc International Inc | Production of fixed glucoseisomerase |
| US5622822A (en) * | 1994-09-13 | 1997-04-22 | Johnson & Johnson Clinical Diagnostics, Inc. | Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1482569A (fr) * | 1966-06-07 | 1967-05-26 | Mobil Oil Corp | Procédé d'oxydation enzymatique de dérivés hydrocarbonés |
| FR1505666A (fr) * | 1966-12-01 | 1967-12-15 | Bristol Myers Co | Procédé de production de l'acide 6-aminopénicillanique par la dégradation enzymatique d'une pénicilline |
-
1973
- 1973-06-18 IE IE100473A patent/IE38174B1/xx unknown
- 1973-06-19 GB GB2913173A patent/GB1411503A/en not_active Expired
- 1973-06-21 FR FR7322608A patent/FR2192114B1/fr not_active Expired
- 1973-06-22 BE BE1005183A patent/BE801351A/xx not_active IP Right Cessation
- 1973-06-29 LU LU67902D patent/LU67902A1/xx unknown
- 1973-07-03 NO NO272773A patent/NO138451C/no unknown
- 1973-07-05 DK DK374273A patent/DK133952B/da not_active IP Right Cessation
- 1973-07-05 NL NL7309439A patent/NL7309439A/xx not_active Application Discontinuation
- 1973-07-06 DE DE19732366417 patent/DE2366417C/de not_active Expired
- 1973-07-06 DE DE19732334521 patent/DE2334521C3/de not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| NL7309439A (enrdf_load_html_response) | 1974-01-09 |
| DE2334521B2 (de) | 1976-12-23 |
| NO138451C (no) | 1978-09-06 |
| DE2334521C3 (de) | 1981-11-19 |
| FR2192114A1 (enrdf_load_html_response) | 1974-02-08 |
| FR2192114B1 (enrdf_load_html_response) | 1979-01-12 |
| GB1411503A (en) | 1975-10-29 |
| DK133952C (enrdf_load_html_response) | 1977-01-24 |
| DE2334521A1 (de) | 1974-01-24 |
| NO138451B (no) | 1978-05-29 |
| DK133952B (da) | 1976-08-16 |
| IE38174L (en) | 1974-01-07 |
| BE801351A (fr) | 1973-10-15 |
| DE2366417C (enrdf_load_html_response) | 1982-07-08 |
| LU67902A1 (enrdf_load_html_response) | 1973-08-31 |
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