IE38174L - Enzyme purification - Google Patents

Enzyme purification

Info

Publication number
IE38174L
IE38174L IE100473A IE100473A IE38174L IE 38174 L IE38174 L IE 38174L IE 100473 A IE100473 A IE 100473A IE 100473 A IE100473 A IE 100473A IE 38174 L IE38174 L IE 38174L
Authority
IE
Ireland
Prior art keywords
surface active
enzyme
cells
precipitate
escherichia coli
Prior art date
Application number
IE100473A
Other versions
IE38174B1 (en
Original Assignee
Samprogetti S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samprogetti S P A filed Critical Samprogetti S P A
Publication of IE38174L publication Critical patent/IE38174L/en
Publication of IE38174B1 publication Critical patent/IE38174B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

1411503 Purifying enzymes SNAMPROGETTI SpA 19 June 1973 [7 July 1972] 29131/73 Heading C3H The proportion of an enzyme in a mixture of (a) the enzyme and (b) at least one other compound, viz. protein, nucleo protein or nucleic acid, produced, e.g. by lysing micro-organism cells, is increased by treating a solution of the mixture with a cationic surface active agent to precipitate (at least partially) the other compound(s) and the precipitate is removed from the treated solution. The preferred surface active agents are hexadecyl pyridinium chloride and benzyl-dimethyl-alkyl (C 10 -C 18 ) ammonium chloride, used in concentrations ranging from 0.01 to 0.4 g/dl. Examples 1 to 6 are directed to the treatment of mixtures rich in penicillin amidase, obtained by homogenizing a suspension of Escherichia coli cells and thereafter removing cell fragments by centrifuging, with one or other of the above specified cationic surface active agents at pH's ranging from 4.5 to 7-0. The purified enzyme could be recovered from the clear supernatant product by addition of ammonium sulphate. Examples 7 and 8 relate to the treatment of mixtures rich in #- galactosidase obtained from cells of Escherichia coli and Saccharomyces lactis, respectively. [GB1411503A]
IE100473A 1972-07-07 1973-06-18 Process for purifying an enzyme IE38174B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2673372 1972-07-07

Publications (2)

Publication Number Publication Date
IE38174L true IE38174L (en) 1974-01-07
IE38174B1 IE38174B1 (en) 1978-01-18

Family

ID=11220129

Family Applications (1)

Application Number Title Priority Date Filing Date
IE100473A IE38174B1 (en) 1972-07-07 1973-06-18 Process for purifying an enzyme

Country Status (9)

Country Link
BE (1) BE801351A (en)
DE (2) DE2366417C (en)
DK (1) DK133952B (en)
FR (1) FR2192114B1 (en)
GB (1) GB1411503A (en)
IE (1) IE38174B1 (en)
LU (1) LU67902A1 (en)
NL (1) NL7309439A (en)
NO (1) NO138451C (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
JPS56109586A (en) * 1980-02-05 1981-08-31 Cpc International Inc Production of fixed glucoseisomerase
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1482569A (en) * 1966-06-07 1967-05-26 Mobil Oil Corp Process for the enzymatic oxidation of hydrocarbon derivatives
FR1505666A (en) * 1966-12-01 1967-12-15 Bristol Myers Co Process for the production of 6-aminopenicillanic acid by the enzymatic degradation of a penicillin

Also Published As

Publication number Publication date
NO138451B (en) 1978-05-29
NO138451C (en) 1978-09-06
DE2366417C (en) 1982-07-08
LU67902A1 (en) 1973-08-31
FR2192114B1 (en) 1979-01-12
DK133952B (en) 1976-08-16
IE38174B1 (en) 1978-01-18
DE2334521C3 (en) 1981-11-19
DE2334521B2 (en) 1976-12-23
NL7309439A (en) 1974-01-09
GB1411503A (en) 1975-10-29
FR2192114A1 (en) 1974-02-08
DK133952C (en) 1977-01-24
DE2334521A1 (en) 1974-01-24
BE801351A (en) 1973-10-15

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