GB1411503A - Process for purfying an enzyme - Google Patents

Process for purfying an enzyme

Info

Publication number
GB1411503A
GB1411503A GB2913173A GB2913173A GB1411503A GB 1411503 A GB1411503 A GB 1411503A GB 2913173 A GB2913173 A GB 2913173A GB 2913173 A GB2913173 A GB 2913173A GB 1411503 A GB1411503 A GB 1411503A
Authority
GB
United Kingdom
Prior art keywords
enzyme
surface active
cells
precipitate
escherichia coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2913173A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SnamProgetti SpA
Original Assignee
SnamProgetti SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SnamProgetti SpA filed Critical SnamProgetti SpA
Publication of GB1411503A publication Critical patent/GB1411503A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

1411503 Purifying enzymes SNAMPROGETTI SpA 19 June 1973 [7 July 1972] 29131/73 Heading C3H The proportion of an enzyme in a mixture of (a) the enzyme and (b) at least one other compound, viz. protein, nucleo protein or nucleic acid, produced, e.g. by lysing micro-organism cells, is increased by treating a solution of the mixture with a cationic surface active agent to precipitate (at least partially) the other compound(s) and the precipitate is removed from the treated solution. The preferred surface active agents are hexadecyl pyridinium chloride and benzyl-dimethyl-alkyl (C 10 -C 18 ) ammonium chloride, used in concentrations ranging from 0.01 to 0.4 g/dl. Examples 1 to 6 are directed to the treatment of mixtures rich in penicillin amidase, obtained by homogenizing a suspension of Escherichia coli cells and thereafter removing cell fragments by centrifuging, with one or other of the above specified cationic surface active agents at pH's ranging from 4.5 to 7-0. The purified enzyme could be recovered from the clear supernatant product by addition of ammonium sulphate. Examples 7 and 8 relate to the treatment of mixtures rich in #- galactosidase obtained from cells of Escherichia coli and Saccharomyces lactis, respectively.
GB2913173A 1972-07-07 1973-06-19 Process for purfying an enzyme Expired GB1411503A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2673372 1972-07-07

Publications (1)

Publication Number Publication Date
GB1411503A true GB1411503A (en) 1975-10-29

Family

ID=11220129

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2913173A Expired GB1411503A (en) 1972-07-07 1973-06-19 Process for purfying an enzyme

Country Status (9)

Country Link
BE (1) BE801351A (en)
DE (2) DE2334521C3 (en)
DK (1) DK133952B (en)
FR (1) FR2192114B1 (en)
GB (1) GB1411503A (en)
IE (1) IE38174B1 (en)
LU (1) LU67902A1 (en)
NL (1) NL7309439A (en)
NO (1) NO138451C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
US4343902A (en) * 1980-02-05 1982-08-10 Cpc International Inc. Production of immobilized glucose isomerase
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1482569A (en) * 1966-06-07 1967-05-26 Mobil Oil Corp Process for the enzymatic oxidation of hydrocarbon derivatives
FR1505666A (en) * 1966-12-01 1967-12-15 Bristol Myers Co Process for the production of 6-aminopenicillanic acid by the enzymatic degradation of a penicillin

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
US4343902A (en) * 1980-02-05 1982-08-10 Cpc International Inc. Production of immobilized glucose isomerase
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same

Also Published As

Publication number Publication date
FR2192114B1 (en) 1979-01-12
DE2334521C3 (en) 1981-11-19
DK133952B (en) 1976-08-16
BE801351A (en) 1973-10-15
DE2366417C (en) 1982-07-08
NO138451B (en) 1978-05-29
DE2334521A1 (en) 1974-01-24
IE38174B1 (en) 1978-01-18
LU67902A1 (en) 1973-08-31
DK133952C (en) 1977-01-24
NO138451C (en) 1978-09-06
DE2334521B2 (en) 1976-12-23
FR2192114A1 (en) 1974-02-08
NL7309439A (en) 1974-01-09
IE38174L (en) 1974-01-07

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Legal Events

Date Code Title Description
PS Patent sealed
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee