IE20011100A1 - Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method - Google Patents
Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such MethodInfo
- Publication number
- IE20011100A1 IE20011100A1 IE20011100A IE20011100A IE20011100A1 IE 20011100 A1 IE20011100 A1 IE 20011100A1 IE 20011100 A IE20011100 A IE 20011100A IE 20011100 A IE20011100 A IE 20011100A IE 20011100 A1 IE20011100 A1 IE 20011100A1
- Authority
- IE
- Ireland
- Prior art keywords
- sample
- alkaline
- sphingomyelinase
- buffer containing
- incubating
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 102100036093 Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 Human genes 0.000 title claims abstract description 11
- 101000876377 Homo sapiens Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 Proteins 0.000 title claims abstract description 11
- 238000004458 analytical method Methods 0.000 title description 5
- 108010061312 Sphingomyelin Phosphodiesterase Proteins 0.000 claims abstract description 8
- 102000011971 Sphingomyelin Phosphodiesterase Human genes 0.000 claims abstract description 8
- 101710124951 Phospholipase C Proteins 0.000 claims description 34
- 101710166827 Sphingomyelinase Proteins 0.000 claims description 34
- 101710122751 Sphingomyelinase C Proteins 0.000 claims description 34
- 239000000523 sample Substances 0.000 claims description 19
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 10
- 238000003556 assay Methods 0.000 claims description 10
- 239000013060 biological fluid Substances 0.000 claims description 10
- DHCLVCXQIBBOPH-UHFFFAOYSA-N Glycerol 2-phosphate Chemical compound OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 239000012131 assay buffer Substances 0.000 claims description 8
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims description 7
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims description 7
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- PKYCWFICOKSIHZ-UHFFFAOYSA-N 1-(3,7-dihydroxyphenoxazin-10-yl)ethanone Chemical compound OC1=CC=C2N(C(=O)C)C3=CC=C(O)C=C3OC2=C1 PKYCWFICOKSIHZ-UHFFFAOYSA-N 0.000 claims description 6
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- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims description 5
- 239000011539 homogenization buffer Substances 0.000 claims description 5
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- 230000007062 hydrolysis Effects 0.000 claims description 5
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- 230000001580 bacterial effect Effects 0.000 claims description 4
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims description 4
- 230000005284 excitation Effects 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 239000011535 reaction buffer Substances 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- HRNLUBSXIHFDHP-UHFFFAOYSA-N N-(2-aminophenyl)-4-[[[4-(3-pyridinyl)-2-pyrimidinyl]amino]methyl]benzamide Chemical compound NC1=CC=CC=C1NC(=O)C(C=C1)=CC=C1CNC1=NC=CC(C=2C=NC=CC=2)=N1 HRNLUBSXIHFDHP-UHFFFAOYSA-N 0.000 claims description 3
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- HSSLDCABUXLXKM-UHFFFAOYSA-N resorufin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3N=C21 HSSLDCABUXLXKM-UHFFFAOYSA-N 0.000 claims description 3
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- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 3
- 229940106189 ceramide Drugs 0.000 description 3
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 3
- 208000029664 classic familial adenomatous polyposis Diseases 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- GHCZAUBVMUEKKP-GYPHWSFCSA-N glycochenodeoxycholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCC(O)=O)C)[C@@]2(C)CC1 GHCZAUBVMUEKKP-GYPHWSFCSA-N 0.000 description 3
- RFDAIACWWDREDC-FRVQLJSFSA-N glycocholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 RFDAIACWWDREDC-FRVQLJSFSA-N 0.000 description 3
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- 108040005466 neutral sphingomyelin phosphodiesterase activity proteins Proteins 0.000 description 3
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- WBWWGRHZICKQGZ-HZAMXZRMSA-N taurocholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS(O)(=O)=O)C)[C@@]2(C)[C@@H](O)C1 WBWWGRHZICKQGZ-HZAMXZRMSA-N 0.000 description 3
- AWDRATDZQPNJFN-VAYUFCLWSA-N taurodeoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS(O)(=O)=O)C)[C@@]2(C)[C@@H](O)C1 AWDRATDZQPNJFN-VAYUFCLWSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57419—Specifically defined cancers of colon
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90206—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (33)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IE20011100A IE20011100A1 (en) | 2001-12-21 | 2001-12-21 | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
| DK02805875T DK1456405T3 (da) | 2001-12-21 | 2002-12-19 | Fremgangsmåde og kit til detektion af alkalisk sphingomyelinase |
| IL16251302A IL162513A0 (en) | 2001-12-21 | 2002-12-19 | Method and kit for detecting alkaline sphingomyelinase |
| ARP020104993A AR038037A1 (es) | 2001-12-21 | 2002-12-19 | Un metodo analitico para detectar esfingomielinasa alcalina y un kit para llevar a cabo el metodo analitico |
| RU2004122426/15A RU2316002C2 (ru) | 2001-12-21 | 2002-12-19 | Аналитический способ обнаружения щелочной сфингомиелиназы и набор, используемый в таком способе |
| PT2805875T PT1456405E (pt) | 2001-12-21 | 2002-12-19 | Método e kit para detecção de esfingomielinase alcalina |
| KR1020047008886A KR101002068B1 (ko) | 2001-12-21 | 2002-12-19 | 알칼리성 스핑고마이엘리나제의 분석학적 검출 방법 및상기 방법에 사용하기 위한 키트 |
| CNA200710147169XA CN101126716A (zh) | 2001-12-21 | 2002-12-19 | 检测碱性鞘磷脂酶的分析方法以及用于该方法的试剂盒 |
| ES02805875T ES2312666T3 (es) | 2001-12-21 | 2002-12-19 | Procedimiento y estuche de ensayo para detectar esfingomielinasa alcalina. |
| HRP20040632AA HRP20040632B1 (hr) | 2001-12-21 | 2002-12-19 | Analitiäśki postupak za otkrivanje alkalne sfingomijelinaze i pribor koji se rabi u ovom postupku |
| US10/499,336 US20050118152A1 (en) | 2001-12-21 | 2002-12-19 | Analytical method for detecting alkaline sphingomyelinase and kit for use in such method |
| HK05107338.1A HK1075069B (en) | 2001-12-21 | 2002-12-19 | Kit for detecting alkaline sphingomyelinase |
| DE60228993T DE60228993D1 (de) | 2001-12-21 | 2002-12-19 | Verfahren und kit zum nachweis von alkalischer sphingomyelinase |
| JP2003556548A JP2005512601A (ja) | 2001-12-21 | 2002-12-19 | アルカリ性スフィンゴミエリナーゼを検出するための分析方法およびかかる方法に使用するキット |
| NZ533806A NZ533806A (en) | 2001-12-21 | 2002-12-19 | Method and kit for detecting alkaline sphingomyelinase |
| BRPI0215045A BRPI0215045B1 (pt) | 2001-12-21 | 2002-12-19 | método analítico para detectar esfingomielinase alcalina e kit para uso em tal método |
| MXPA04005919A MXPA04005919A (es) | 2001-12-21 | 2002-12-19 | Metodo analitico para detectar efingomielinasa alcalina y kit para uso en este metodo. |
| HU0402542A HU228812B1 (hu) | 2001-12-21 | 2002-12-19 | Analitikai eljárás bázisos szfingomielináz detektálására és az ennek alkalmazására szolgáló kit |
| AU2002367123A AU2002367123B2 (en) | 2001-12-21 | 2002-12-19 | Method and kit for detecting alkaline sphingomyelinase |
| EP02805875A EP1456405B1 (en) | 2001-12-21 | 2002-12-19 | Method and kit for detecting alkaline sphingomyelinase |
| PCT/IT2002/000811 WO2003056031A2 (en) | 2001-12-21 | 2002-12-19 | Method and kit for detecting alkaline sphingomyelinase |
| SI200230740T SI1456405T1 (sl) | 2001-12-21 | 2002-12-19 | Postopek in set za določanje alkalne sfingomielinaze |
| CA2469796A CA2469796C (en) | 2001-12-21 | 2002-12-19 | Analytical method for detecting alkaline sphingomyelinase and kit for use in such method |
| PL374085A PL207876B1 (pl) | 2001-12-21 | 2002-12-19 | Analityczny sposób wykrywania alkalicznej sfingomielinazy oraz zestaw do zastosowania w tym sposobie |
| AT02805875T ATE408707T1 (de) | 2001-12-21 | 2002-12-19 | Verfahren und kit zum nachweis von alkalischer sphingomyelinase |
| EG2002121379A EG25804A (en) | 2001-12-21 | 2002-12-21 | A kit for detecting sphingomyelinase in a biological material |
| IL162513A IL162513A (en) | 2001-12-21 | 2004-06-14 | Method and kit for detecting alkaline sphingomyelinase |
| NO20042992A NO331806B1 (no) | 2001-12-21 | 2004-07-13 | Analytisk fremgangsmate for in vitro deteksjon av alkalisk sfingomyelinase og kit for anvendelse ved slik fremgangsmate |
| ZA200405762A ZA200405762B (en) | 2001-12-21 | 2004-07-20 | Method and kit for detecting alkaline sphingomyelinase |
| US11/359,619 US7211410B2 (en) | 2001-12-21 | 2006-02-23 | Analytical method for detecting alkaline sphingomyelinase and kit for use in such method |
| US11/712,417 US7323317B2 (en) | 2001-12-21 | 2007-03-01 | Analytical method for detecting alkaline sphingomyelinase and kit for use in such method |
| CY20081101308T CY1108512T1 (el) | 2001-12-21 | 2008-11-13 | Μεθοδος και τυποποιημενη συσκευασια για ανιχνευση της αλκαλικης σφιγγομυελινασης |
| JP2009104135A JP5009332B2 (ja) | 2001-12-21 | 2009-04-22 | アルカリ性スフィンゴミエリナーゼを検出するための分析方法およびかかる方法に使用するキット |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IE20011100A IE20011100A1 (en) | 2001-12-21 | 2001-12-21 | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IE20011100A1 true IE20011100A1 (en) | 2003-07-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE20011100A IE20011100A1 (en) | 2001-12-21 | 2001-12-21 | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
Country Status (28)
| Country | Link |
|---|---|
| US (3) | US20050118152A1 (enExample) |
| EP (1) | EP1456405B1 (enExample) |
| JP (2) | JP2005512601A (enExample) |
| KR (1) | KR101002068B1 (enExample) |
| CN (1) | CN101126716A (enExample) |
| AR (1) | AR038037A1 (enExample) |
| AT (1) | ATE408707T1 (enExample) |
| AU (1) | AU2002367123B2 (enExample) |
| BR (1) | BRPI0215045B1 (enExample) |
| CA (1) | CA2469796C (enExample) |
| CY (1) | CY1108512T1 (enExample) |
| DE (1) | DE60228993D1 (enExample) |
| DK (1) | DK1456405T3 (enExample) |
| EG (1) | EG25804A (enExample) |
| ES (1) | ES2312666T3 (enExample) |
| HR (1) | HRP20040632B1 (enExample) |
| HU (1) | HU228812B1 (enExample) |
| IE (1) | IE20011100A1 (enExample) |
| IL (2) | IL162513A0 (enExample) |
| MX (1) | MXPA04005919A (enExample) |
| NO (1) | NO331806B1 (enExample) |
| NZ (1) | NZ533806A (enExample) |
| PL (1) | PL207876B1 (enExample) |
| PT (1) | PT1456405E (enExample) |
| RU (1) | RU2316002C2 (enExample) |
| SI (1) | SI1456405T1 (enExample) |
| WO (1) | WO2003056031A2 (enExample) |
| ZA (1) | ZA200405762B (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE20011100A1 (en) | 2001-12-21 | 2003-07-09 | Vsl Pharma Ltd | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
| PL1973561T3 (pl) * | 2005-12-15 | 2011-08-31 | Elanco Us Inc | Enzymy do zmniejszania stresu immunologicznego |
| CN103760348B (zh) * | 2014-02-11 | 2015-03-11 | 苏州博源医疗科技有限公司 | 一种甘胆酸免疫检测试剂及其制备和检测方法 |
| CN104614371A (zh) * | 2015-02-15 | 2015-05-13 | 史春龙 | 一种在便器中检测排泄物生化指标的方法及使用该方法的检测设备 |
| CN116106281A (zh) * | 2023-02-03 | 2023-05-12 | 大连医科大学 | 一种粪便样本中肠道菌脂肪酶活性的检测方法 |
| CN117630364B (zh) * | 2023-12-05 | 2024-07-30 | 临沂大学 | 一种双酶型免疫荧光系统检测痕量黄曲霉毒素b1的方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1311495B1 (it) * | 1999-06-09 | 2002-03-13 | Mendes S U R L | Composizione comprendente sfingomielinasi alcalina, utilizzabile qualeprodotto dietetico, integratore alimentare o medicamento. |
| US6265179B1 (en) * | 2000-02-01 | 2001-07-24 | Molecular Probes, Inc. | Detection of phosphate using coupled enzymatic reactions |
| IE20011100A1 (en) | 2001-12-21 | 2003-07-09 | Vsl Pharma Ltd | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
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- 2002-12-19 AT AT02805875T patent/ATE408707T1/de active
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- 2002-12-19 IL IL16251302A patent/IL162513A0/xx unknown
- 2002-12-19 AU AU2002367123A patent/AU2002367123B2/en not_active Ceased
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| MM9A | Patent lapsed through non-payment of renewal fee |