HUE028676T2 - Májprogenitor-sejtek kondicionált tápközege - Google Patents
Májprogenitor-sejtek kondicionált tápközege Download PDFInfo
- Publication number
- HUE028676T2 HUE028676T2 HUE09761761A HUE09761761A HUE028676T2 HU E028676 T2 HUE028676 T2 HU E028676T2 HU E09761761 A HUE09761761 A HU E09761761A HU E09761761 A HUE09761761 A HU E09761761A HU E028676 T2 HUE028676 T2 HU E028676T2
- Authority
- HU
- Hungary
- Prior art keywords
- cells
- rti
- growth factor
- human
- conditioned medium
- Prior art date
Links
- 239000003636 conditioned culture medium Substances 0.000 title claims description 92
- 210000004185 liver Anatomy 0.000 title claims description 50
- 210000000130 stem cell Anatomy 0.000 title description 38
- 210000004027 cell Anatomy 0.000 claims description 64
- 239000000203 mixture Substances 0.000 claims description 56
- 239000008194 pharmaceutical composition Substances 0.000 claims description 31
- 102000004169 proteins and genes Human genes 0.000 claims description 31
- 108090000623 proteins and genes Proteins 0.000 claims description 31
- 210000003494 hepatocyte Anatomy 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 19
- 239000003102 growth factor Substances 0.000 claims description 13
- 210000000056 organ Anatomy 0.000 claims description 9
- 230000012010 growth Effects 0.000 claims description 8
- 208000027418 Wounds and injury Diseases 0.000 claims description 7
- 230000006378 damage Effects 0.000 claims description 7
- 208000014674 injury Diseases 0.000 claims description 7
- 230000008472 epithelial growth Effects 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 210000003734 kidney Anatomy 0.000 claims description 2
- 206010002091 Anaesthesia Diseases 0.000 claims 1
- 208000019901 Anxiety disease Diseases 0.000 claims 1
- 241000287828 Gallus gallus Species 0.000 claims 1
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000010441 alabaster Substances 0.000 claims 1
- 230000037005 anaesthesia Effects 0.000 claims 1
- 239000004599 antimicrobial Substances 0.000 claims 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 claims 1
- 230000035622 drinking Effects 0.000 claims 1
- 210000000981 epithelium Anatomy 0.000 claims 1
- 230000005713 exacerbation Effects 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 241000411851 herbal medicine Species 0.000 claims 1
- 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 230000032646 lung growth Effects 0.000 claims 1
- 230000003211 malignant effect Effects 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 150000004682 monohydrates Chemical class 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 230000011514 reflex Effects 0.000 claims 1
- 239000013589 supplement Substances 0.000 claims 1
- 210000005166 vasculature Anatomy 0.000 claims 1
- MSWZFWKMSRAUBD-GASJEMHNSA-N 2-amino-2-deoxy-D-galactopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O MSWZFWKMSRAUBD-GASJEMHNSA-N 0.000 description 50
- 102000004127 Cytokines Human genes 0.000 description 44
- 108090000695 Cytokines Proteins 0.000 description 44
- 239000002158 endotoxin Substances 0.000 description 37
- 102000004889 Interleukin-6 Human genes 0.000 description 36
- 108090001005 Interleukin-6 Proteins 0.000 description 36
- 102000004890 Interleukin-8 Human genes 0.000 description 36
- 108090001007 Interleukin-8 Proteins 0.000 description 36
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 35
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 35
- 229920006008 lipopolysaccharide Polymers 0.000 description 34
- 229940100601 interleukin-6 Drugs 0.000 description 33
- 229940096397 interleukin-8 Drugs 0.000 description 33
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 description 33
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 32
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 31
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 30
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 30
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 30
- 235000018102 proteins Nutrition 0.000 description 27
- 239000002609 medium Substances 0.000 description 24
- 238000002474 experimental method Methods 0.000 description 22
- 102000049853 macrophage stimulating protein Human genes 0.000 description 22
- 108010053292 macrophage stimulating protein Proteins 0.000 description 22
- 208000007788 Acute Liver Failure Diseases 0.000 description 20
- 206010000804 Acute hepatic failure Diseases 0.000 description 20
- 238000011579 SCID mouse model Methods 0.000 description 20
- 239000007924 injection Substances 0.000 description 19
- 238000002347 injection Methods 0.000 description 19
- 108091006905 Human Serum Albumin Proteins 0.000 description 17
- 102000008100 Human Serum Albumin Human genes 0.000 description 17
- 241001465754 Metazoa Species 0.000 description 16
- 241000699670 Mus sp. Species 0.000 description 16
- 238000002360 preparation method Methods 0.000 description 16
- 210000002966 serum Anatomy 0.000 description 16
- 238000012258 culturing Methods 0.000 description 14
- 239000006228 supernatant Substances 0.000 description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- 230000004083 survival effect Effects 0.000 description 12
- 238000001727 in vivo Methods 0.000 description 11
- 238000000108 ultra-filtration Methods 0.000 description 11
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 10
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 10
- 101000958041 Homo sapiens Musculin Proteins 0.000 description 10
- 230000001172 regenerating effect Effects 0.000 description 10
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 9
- 238000003556 assay Methods 0.000 description 9
- 210000004271 bone marrow stromal cell Anatomy 0.000 description 9
- 230000006698 induction Effects 0.000 description 9
- 231100000518 lethal Toxicity 0.000 description 9
- 230000001665 lethal effect Effects 0.000 description 9
- 239000012528 membrane Substances 0.000 description 9
- 238000011282 treatment Methods 0.000 description 9
- 108010003415 Aspartate Aminotransferases Proteins 0.000 description 8
- 102000004625 Aspartate Aminotransferases Human genes 0.000 description 8
- 230000006907 apoptotic process Effects 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 description 7
- 108010082126 Alanine transaminase Proteins 0.000 description 7
- 108010057081 Merozoite Surface Protein 1 Proteins 0.000 description 7
- 210000001185 bone marrow Anatomy 0.000 description 7
- 230000003247 decreasing effect Effects 0.000 description 7
- 239000003814 drug Substances 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 238000010186 staining Methods 0.000 description 7
- 238000001262 western blot Methods 0.000 description 7
- QAPSNMNOIOSXSQ-YNEHKIRRSA-N 1-[(2r,4s,5r)-4-[tert-butyl(dimethyl)silyl]oxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O[Si](C)(C)C(C)(C)C)C1 QAPSNMNOIOSXSQ-YNEHKIRRSA-N 0.000 description 6
- 108050006400 Cyclin Proteins 0.000 description 6
- 102100036691 Proliferating cell nuclear antigen Human genes 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 230000034994 death Effects 0.000 description 6
- 231100000517 death Toxicity 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000028974 hepatocyte apoptotic process Effects 0.000 description 6
- 239000007928 intraperitoneal injection Substances 0.000 description 6
- 206010028851 Necrosis Diseases 0.000 description 5
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 239000006143 cell culture medium Substances 0.000 description 5
- 230000030833 cell death Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000017074 necrotic cell death Effects 0.000 description 5
- 108010063104 Apoptosis Regulatory Proteins Proteins 0.000 description 4
- 102000010565 Apoptosis Regulatory Proteins Human genes 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 206010019663 Hepatic failure Diseases 0.000 description 4
- 206010053159 Organ failure Diseases 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 238000010253 intravenous injection Methods 0.000 description 4
- 208000007903 liver failure Diseases 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000009010 Bradford assay Methods 0.000 description 3
- 102100021943 C-C motif chemokine 2 Human genes 0.000 description 3
- 102000019034 Chemokines Human genes 0.000 description 3
- 108010012236 Chemokines Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102100037362 Fibronectin Human genes 0.000 description 3
- 108010067306 Fibronectins Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 206010021143 Hypoxia Diseases 0.000 description 3
- 108091058560 IL8 Proteins 0.000 description 3
- 101710091439 Major capsid protein 1 Proteins 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 102000008108 Osteoprotegerin Human genes 0.000 description 3
- 108010035042 Osteoprotegerin Proteins 0.000 description 3
- 208000001647 Renal Insufficiency Diseases 0.000 description 3
- 230000001640 apoptogenic effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000012228 culture supernatant Substances 0.000 description 3
- 238000011496 digital image analysis Methods 0.000 description 3
- 235000013861 fat-free Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000001146 hypoxic effect Effects 0.000 description 3
- 201000006370 kidney failure Diseases 0.000 description 3
- 231100000225 lethality Toxicity 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 238000010899 nucleation Methods 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000005199 ultracentrifugation Methods 0.000 description 3
- IMGXDCAOBSTOQJ-CFTXMQDWSA-N (3R,4R,5R,6R)-3-amino-6-(hydroxymethyl)oxane-2,4,5-triol (2R,3R,4R,5R)-2-amino-3,4,5,6-tetrahydroxyhexanal Chemical compound O=C[C@H](N)[C@@H](O)[C@@H](O)[C@H](O)CO.N[C@H]1C(O)O[C@H](CO)[C@H](O)[C@@H]1O IMGXDCAOBSTOQJ-CFTXMQDWSA-N 0.000 description 2
- 108010075348 Activated-Leukocyte Cell Adhesion Molecule Proteins 0.000 description 2
- 108010039627 Aprotinin Proteins 0.000 description 2
- 101150008012 Bcl2l1 gene Proteins 0.000 description 2
- 102100037853 C-C chemokine receptor type 4 Human genes 0.000 description 2
- 102100024210 CD166 antigen Human genes 0.000 description 2
- 102000005754 Cytokine Receptor gp130 Human genes 0.000 description 2
- 108010006197 Cytokine Receptor gp130 Proteins 0.000 description 2
- 102000006975 Ectodysplasins Human genes 0.000 description 2
- 108010072589 Ectodysplasins Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 2
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 2
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 description 2
- 101000599951 Homo sapiens Insulin-like growth factor I Proteins 0.000 description 2
- 101000976913 Homo sapiens Lens fiber major intrinsic protein Proteins 0.000 description 2
- 238000012404 In vitro experiment Methods 0.000 description 2
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 2
- 102000004883 Insulin-like growth factor-binding protein 6 Human genes 0.000 description 2
- 108090001014 Insulin-like growth factor-binding protein 6 Proteins 0.000 description 2
- 102100023487 Lens fiber major intrinsic protein Human genes 0.000 description 2
- GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 2
- 206010067125 Liver injury Diseases 0.000 description 2
- 102100039867 Outer mitochondrial transmembrane helix translocase Human genes 0.000 description 2
- -1 PDGF Proteins 0.000 description 2
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 2
- 238000012288 TUNEL assay Methods 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229960004405 aprotinin Drugs 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000003828 downregulation Effects 0.000 description 2
- 229940082150 encore Drugs 0.000 description 2
- 102000046949 human MSC Human genes 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 2
- GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 2
- 108010052968 leupeptin Proteins 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000009818 osteogenic differentiation Effects 0.000 description 2
- 102000013415 peroxidase activity proteins Human genes 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000019624 protein content Nutrition 0.000 description 2
- 235000002639 sodium chloride Nutrition 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000003827 upregulation Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- WIGIZIANZCJQQY-UHFFFAOYSA-N 4-ethyl-3-methyl-N-[2-[4-[[[(4-methylcyclohexyl)amino]-oxomethyl]sulfamoyl]phenyl]ethyl]-5-oxo-2H-pyrrole-1-carboxamide Chemical compound O=C1C(CC)=C(C)CN1C(=O)NCCC1=CC=C(S(=O)(=O)NC(=O)NC2CCC(C)CC2)C=C1 WIGIZIANZCJQQY-UHFFFAOYSA-N 0.000 description 1
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 102000005606 Activins Human genes 0.000 description 1
- 108010059616 Activins Proteins 0.000 description 1
- 108010078606 Adipokines Proteins 0.000 description 1
- 102000014777 Adipokines Human genes 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 235000011330 Armoracia rusticana Nutrition 0.000 description 1
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 241000195940 Bryophyta Species 0.000 description 1
- 101710149863 C-C chemokine receptor type 4 Proteins 0.000 description 1
- 102100025248 C-X-C motif chemokine 10 Human genes 0.000 description 1
- 101710098275 C-X-C motif chemokine 10 Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000001326 Chemokine CCL4 Human genes 0.000 description 1
- 108010055165 Chemokine CCL4 Proteins 0.000 description 1
- 102000001327 Chemokine CCL5 Human genes 0.000 description 1
- 108010055166 Chemokine CCL5 Proteins 0.000 description 1
- 108010071942 Colony-Stimulating Factors Proteins 0.000 description 1
- 102100032759 Cysteine-rich motor neuron 1 protein Human genes 0.000 description 1
- 108090000738 Decorin Proteins 0.000 description 1
- 102000004237 Decorin Human genes 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 108050009340 Endothelin Proteins 0.000 description 1
- 102000002045 Endothelin Human genes 0.000 description 1
- 102100023688 Eotaxin Human genes 0.000 description 1
- 101710139422 Eotaxin Proteins 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 102100026149 Fibroblast growth factor receptor-like 1 Human genes 0.000 description 1
- 101710084357 Fibroblast growth factor receptor-like 1 Proteins 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 102000010956 Glypican Human genes 0.000 description 1
- 108050001154 Glypican Proteins 0.000 description 1
- 108050007237 Glypican-3 Proteins 0.000 description 1
- 241001481828 Glyptocephalus cynoglossus Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000738584 Homo sapiens C-C chemokine receptor type 4 Proteins 0.000 description 1
- 101000942095 Homo sapiens Cysteine-rich motor neuron 1 protein Proteins 0.000 description 1
- 101001044893 Homo sapiens Interleukin-20 receptor subunit alpha Proteins 0.000 description 1
- 101001055222 Homo sapiens Interleukin-8 Proteins 0.000 description 1
- 101000604886 Homo sapiens Kremen protein 2 Proteins 0.000 description 1
- 102000000589 Interleukin-1 Human genes 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 102000003777 Interleukin-1 beta Human genes 0.000 description 1
- 108090000193 Interleukin-1 beta Proteins 0.000 description 1
- 108090000174 Interleukin-10 Proteins 0.000 description 1
- 102000003816 Interleukin-13 Human genes 0.000 description 1
- 108090000176 Interleukin-13 Proteins 0.000 description 1
- 102000003812 Interleukin-15 Human genes 0.000 description 1
- 108090000172 Interleukin-15 Proteins 0.000 description 1
- 102000013691 Interleukin-17 Human genes 0.000 description 1
- 108050003558 Interleukin-17 Proteins 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 102100022706 Interleukin-20 receptor subunit alpha Human genes 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 108010002616 Interleukin-5 Proteins 0.000 description 1
- 108010002586 Interleukin-7 Proteins 0.000 description 1
- 102100026236 Interleukin-8 Human genes 0.000 description 1
- 108010002335 Interleukin-9 Proteins 0.000 description 1
- 101710177504 Kit ligand Proteins 0.000 description 1
- 102100038224 Kremen protein 2 Human genes 0.000 description 1
- 102100027000 Latent-transforming growth factor beta-binding protein 1 Human genes 0.000 description 1
- 101710178954 Latent-transforming growth factor beta-binding protein 1 Proteins 0.000 description 1
- 108060004872 MIF Proteins 0.000 description 1
- 108010046938 Macrophage Colony-Stimulating Factor Proteins 0.000 description 1
- 102000007651 Macrophage Colony-Stimulating Factor Human genes 0.000 description 1
- 102000009571 Macrophage Inflammatory Proteins Human genes 0.000 description 1
- 108010009474 Macrophage Inflammatory Proteins Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000009890 Osteonectin Human genes 0.000 description 1
- 108010077077 Osteonectin Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000012083 RIPA buffer Substances 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102100037599 SPARC Human genes 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 1
- 102100040247 Tumor necrosis factor Human genes 0.000 description 1
- CYKLRRKFBPBYEI-KBQKSTHMSA-N UDP-alpha-D-galactosamine Chemical class O1[C@H](CO)[C@H](O)[C@H](O)[C@@H](N)[C@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 CYKLRRKFBPBYEI-KBQKSTHMSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000478 adipokine Substances 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000002870 angiogenesis inducing agent Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 239000012152 bradford reagent Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001889 chemoattractive effect Effects 0.000 description 1
- 239000007376 cm-medium Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000816 effect on animals Effects 0.000 description 1
- ZUBDGKVDJUIMQQ-UBFCDGJISA-N endothelin-1 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@H]2CSSC[C@@H](C(N[C@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@@H](CO)NC(=O)[C@H](N)CSSC1)C1=CNC=N1 ZUBDGKVDJUIMQQ-UBFCDGJISA-N 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 231100000753 hepatic injury Toxicity 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 230000002443 hepatoprotective effect Effects 0.000 description 1
- 230000003082 hepatotoxic effect Effects 0.000 description 1
- 238000007489 histopathology method Methods 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 230000004968 inflammatory condition Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 108090000681 interleukin 20 Proteins 0.000 description 1
- 102000004114 interleukin 20 Human genes 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 238000007837 multiplex assay Methods 0.000 description 1
- XXUPLYBCNPLTIW-UHFFFAOYSA-N octadec-7-ynoic acid Chemical compound CCCCCCCCCCC#CCCCCCC(O)=O XXUPLYBCNPLTIW-UHFFFAOYSA-N 0.000 description 1
- 102000027450 oncoproteins Human genes 0.000 description 1
- 108091008819 oncoproteins Proteins 0.000 description 1
- 230000033667 organ regeneration Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 238000003498 protein array Methods 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 238000011552 rat model Methods 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000002660 stem cell treatment Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/407—Liver; Hepatocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/177—Receptors; Cell surface antigens; Cell surface determinants
- A61K38/1793—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
- A61K38/1833—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
- A61K38/1858—Platelet-derived growth factor [PDGF]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
- A61K38/1858—Platelet-derived growth factor [PDGF]
- A61K38/1866—Vascular endothelial growth factor [VEGF]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/204—IL-6
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/19—Cytokines; Lymphokines; Interferons
- A61K38/20—Interleukins [IL]
- A61K38/2053—IL-8
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/30—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/80—Undefined extracts from animals
- C12N2500/84—Undefined extracts from animals from mammals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physiology (AREA)
- Vascular Medicine (AREA)
- Marine Sciences & Fisheries (AREA)
- Nutrition Science (AREA)
- Virology (AREA)
- Diabetes (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Urology & Nephrology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (4)
- MÁJlPkOGENTFö8--SETr£KKGM)S€ION’ÁLT TÁPKÖ&EGE Szabadalmi sgérsyposuok t Eljárás kmécíoMtitúpközeg előállítására, amely tótee a következő lépésekét: (1) féfeöfk ihájeredeti:, feft hepatocíták tégyósdéss sejttenyesztö tágtózégbén a» érett lepstociták: «Ipasztnlásmg és epltheloid tnortslogiájü, táMiö popaMctójásak sxefefepása; Síi:) a iáiéiík ζβφφίΜ morfölégsajn sejtek popatáesiéjáKak ex;pandáiása $^m®&&hxéy glSkóztamltnó, bBGF-fel (intml® epithéiialis növekedési faktor) és bFGF-iél (bszikus öhtt>hiaszí-rk>vekedési faktor) kiegésziteit és - endösseitek. szaporodásához szükséges^ szokásos szerveden sókat, aoáposa^akaf és vitaminokat tartalmazó ~ tápközegbentörténő tenyésztéssé!; és (isi) a sejtok elMMmtese a «gttenyésztö tépkózegbóh .2, Az L ígéoypoot saeíkaí: eJ|;ás-ás:, «bel az érett hepaiooiták skéfomtaríaMá tsstyészíö í%)közeghep lefagyasztássa kemjtsk knppzőíoktté «kér jelenlétében, majd M íáöek olvasztva,.m (f> lépést $m:má íersyóktáés élőt;.
- 3. ÁM !, vágy 2. igénypont szerinti éljárás, áltól a tenyésztő itápköseg stÍb~MEM~st: tartab&as OMF minőségé humán aibereinnal kiegészítve.
- 4. Barnán, plorípoteas májprogenifor^tvonaf tóswésjlésés®t efSáítthatö, kondicionált tápkózsg:» azzal Jelentem,, hogy sejtekről merne*. továáM azzal Jefomezve, hogy legalább a kővetkezők keverékét tartalmazza: hepatoeita növekedési fater piGFj, intetisukm # Gk-d), intsrleukhr &' $1,-4) és vaszkolade emfothehalis növekedési faktor (VBGf). 5. A 4. igénylőt szerinti, kondicionált tápközegkőszitmény, amely 1^3, igénypontok bármelyike szerinti eljárással állítható elő. b. Gyógyászati késziimétsy, amely a 4, vagy 5. igénypont szerinti, krmdicionáR tápki>zeg gyógyászadlag Itatásos mennyiségét tartalmazza. ?. Gyógyászati :késKíttoeöy, amely legalább a kÓveÉezok keverékének gyogyásxsbiag hatásos sieaoyiségéi tartalmazza: hepatövita növekedési faktor (HGF'k inmdeykin ó :(11- -5), iafeílehkiti 8 (11..-8) ék vasiíu.lárís endoA«álá^.»övá^&si f&fo* (VEGFh 10-400 og/tni koneentfáciid-íartonsánybáii. 8, A 1. igénypoid szerinti; gyógyászati készöínény, amelyben a keverek üsmímm továbbá: jbókirjflg-shsiplálö proteint (MSP) gj^gyáskóttlag aktív metmyiségben. 9. A 7. vagy 8, Igénypótri stebti gyógyászati: ;készt»lny? amelyben a hepatoölg: nóveksáM :Mder (IIGF) 1-100 ngjfni konoesitráotátmtomátrynan vad jeled,: az interienkin 6 (ILrh) 10-200 .álltái: .keneéöttáoíótartoínánybsí! vart: jéien, az aierienkin 8 (lL-8) 35 ng/rsl vttgy jnagasabb kon«éhtóéióban van: jelen. :1;0. A 8. vagy 9,. igénypont szerinti gyógyászati készítmény, amelyben a nrakxofágsíimaiák) protein. (MS?) í'HKi pg/ml komzmszádbtsnománybim van jden. 1.1;. MvnmriMM gmgvmx^ü Msmtmmy, apóly a 9, vagy 1f, sgéeypöaí szermii legalább IMzöröséte, előnyösen legalább 1 ö-ass^sire, előnyösebben legalább 2Cv-szaro$áfa, pég eíönyö^bbm legnMbli 15*szörösére koncentrál gyógyászati készítményből áil.
- 12. Hígított pegyaasaíi készitefe, amely a 9, mgy 10. igénypont szerinti, lepMbb S-szikosöre, éOPyösen legalább it-saepsére, előnyösebben tepiább2Ct-szoro$ára, pég előnyösebben Ssplabb 25~szörősé?e higiííjt I; gyégpssab MsáÓPténybŐl Ml 13. A 6-12. igénypsösiok bármelyike szerinti gyógyászati készítmény szerv sérülésének vagy eiégíelenaögénskikeKeléséibeá válé alkalmazásra, 14. Á 13. igénypont szedni! gybgyásssál késKÍönény aa öl* megimtlrtmöií aikabasaásrs, shel s szerv máf vagy vese. 15. A 13. vagy 14. igénypont szerinti :gy%yászaii készítmény az őst megbatározott alkalmazásra. amely Ő:,Ö!~] mgíkg ílózlsíadöikásyban bepttoeia növekedési faktor píGt), 0,01··! rn|íkg dözlstprtományb&ö: mierleókin 6 (ÍL~% 0,044 mg% dé^staríopánybait táteiieufein % (IL-Ó) és adott esetben Ö,ÖÍ 4 stgdsg döajstanoményban vsszkniárss «mloipialts növekedési faktor (VEHF} beadására tdktőpas gyö^sssitótt elöálbtött
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP08010651 | 2008-06-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HUE028676T2 true HUE028676T2 (hu) | 2016-12-28 |
Family
ID=40905279
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HUE09761761A HUE028676T2 (hu) | 2008-06-11 | 2009-06-11 | Májprogenitor-sejtek kondicionált tápközege |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US10456425B2 (hu) |
| EP (1) | EP2296672B1 (hu) |
| JP (1) | JP5632833B2 (hu) |
| CN (2) | CN103800895B (hu) |
| BR (1) | BRPI0909866B1 (hu) |
| CA (1) | CA2727392C (hu) |
| DK (1) | DK2296672T3 (hu) |
| ES (1) | ES2556630T3 (hu) |
| HU (1) | HUE028676T2 (hu) |
| PL (1) | PL2296672T3 (hu) |
| PT (1) | PT2296672E (hu) |
| WO (1) | WO2009150199A1 (hu) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2333047A1 (en) * | 2009-12-09 | 2011-06-15 | Fresenius Medical Care Deutschland GmbH | Adult stem cell derived conditioned medium and/or adult stem cells for use in the therapeutic treatment of a tumor disease |
| US8603470B1 (en) | 2012-08-07 | 2013-12-10 | National Cheng Kung University | Use of IL-20 antagonists for treating liver diseases |
| JP6474394B2 (ja) * | 2013-07-05 | 2019-02-27 | ユニベルシテ カトリック ドゥ ルーベン | ヒト成体肝臓幹細胞由来の馴化培地および肝臓障害の治療におけるその使用 |
| AR099641A1 (es) | 2013-09-05 | 2016-08-10 | Consejo Nac De Investig Científicas Y Técnicas (Conicet) | OSTEONECTINA (SPARC) COMO BLANCO TERAPÉUTICO PARA EL TRATAMIENTO Y PREVENCIÓN DEL FALLO HEPÁTICO AGUDO, COMPOSICIÓN FARMACEUTICA, CONSTRUCTO GENÉTICO, ARNsi, AGENTE QUE INHIBE LA EXPRESIÓN, USO |
| RU2644650C2 (ru) * | 2014-12-01 | 2018-02-13 | Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" | Материал стволовых клеток и способ его получения |
| CN104818245B (zh) * | 2015-05-29 | 2020-02-04 | 广州赛莱拉干细胞科技股份有限公司 | 一种肝脏干细胞的培养基及培养方法 |
| KR20180108789A (ko) | 2016-02-10 | 2018-10-04 | 웨이크 포리스트 유니버시티 헬스 사이언시즈 | 간 섬유화의 모델 시스템 및 그의 제조 및 사용 방법 |
| RU2708329C2 (ru) | 2016-05-31 | 2019-12-05 | Общество с ограниченной ответственностью "Т-Хелпер Клеточные Технологии" | Материал стволовых клеток, композиции и способы применения |
| CN109628377A (zh) * | 2019-01-02 | 2019-04-16 | 贵州省人民医院 | 一种小鼠原代肝细胞灌注式分离及离体培养方法 |
| CN111607556B (zh) * | 2019-01-25 | 2022-06-07 | 中国科学院广州生物医药与健康研究院 | 一种培养扩增人肝祖细胞的培养基及其应用 |
| TW202106875A (zh) * | 2019-04-30 | 2021-02-16 | 比利時商普羅米修亞生物科技股份有限公司 | 人類同種異體肝衍生前驅細胞的製備(二) |
| CN111394391B (zh) * | 2019-07-11 | 2022-12-06 | 上海赛立维生物科技有限公司 | 肝祖细胞样细胞库的构建方法及其制备的细胞株与应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH093099A (ja) * | 1995-06-20 | 1997-01-07 | Toyobo Co Ltd | マクロファージ刺激蛋白質改変体およびその製造法 |
| US6475490B1 (en) * | 1998-10-19 | 2002-11-05 | Fordham University | Compositions and methods for promoting tissue repair using heat shock proteins |
| US6372494B1 (en) * | 1999-05-14 | 2002-04-16 | Advanced Tissue Sciences, Inc. | Methods of making conditioned cell culture medium compositions |
| US6719969B1 (en) * | 1999-08-09 | 2004-04-13 | The Regents Of The University Of Michigan | Treatment of liver disease and injury with CXC chemokines |
| CA2290240C (en) | 1999-11-24 | 2008-03-11 | Stergios Stergiopoulos | High resolution 3d ultrasound imaging system deploying a multi-dimensional array of sensors and method for multi-dimensional beamforming sensor signals |
| US7547674B2 (en) * | 2001-06-06 | 2009-06-16 | New York Medical College | Methods and compositions for the repair and/or regeneration of damaged myocardium |
| US6916770B2 (en) | 2001-04-27 | 2005-07-12 | Polyone Corporation | Multi-functional color concentrate compositions |
| WO2002098365A2 (en) * | 2001-06-07 | 2002-12-12 | Skinmedica, Inc. | Conditioned cell culture media and uses thereof |
| WO2003033697A1 (en) * | 2001-10-18 | 2003-04-24 | Ixion Biotechnology, Inc. | Conversion of liver stem and progenitor cells to pancreatic functional cells |
| WO2006126219A1 (en) * | 2005-05-26 | 2006-11-30 | Fresenius Medical Care Deutschland G.M.B.H. | Liver progenitor cells |
| WO2007097993A2 (en) * | 2006-02-16 | 2007-08-30 | The Burnham Institute Of Medical Research | Media conditioned by human embryonic stem cells or other progenitor cells and uses therefor |
| KR101505202B1 (ko) * | 2006-03-07 | 2015-03-27 | 지타 쉬로프 | 인간 배아 줄기 세포 및 그의 유도체를 포함하는 조성물, 그의 사용 방법 및 제조 방법 |
| FR2912916B1 (fr) * | 2007-02-26 | 2009-05-22 | Oreal | Composition cosmetique ou dermatologique comprenant un milieu de culture cellulaire |
-
2009
- 2009-06-11 JP JP2011512983A patent/JP5632833B2/ja active Active
- 2009-06-11 BR BRPI0909866-6A patent/BRPI0909866B1/pt active IP Right Grant
- 2009-06-11 DK DK09761761.7T patent/DK2296672T3/en active
- 2009-06-11 CA CA2727392A patent/CA2727392C/en active Active
- 2009-06-11 PL PL09761761T patent/PL2296672T3/pl unknown
- 2009-06-11 HU HUE09761761A patent/HUE028676T2/hu unknown
- 2009-06-11 EP EP09761761.7A patent/EP2296672B1/en active Active
- 2009-06-11 US US12/737,143 patent/US10456425B2/en active Active
- 2009-06-11 WO PCT/EP2009/057232 patent/WO2009150199A1/en active Application Filing
- 2009-06-11 ES ES09761761.7T patent/ES2556630T3/es active Active
- 2009-06-11 CN CN201310348088.1A patent/CN103800895B/zh active Active
- 2009-06-11 PT PT97617617T patent/PT2296672E/pt unknown
- 2009-06-11 CN CN200980131229.6A patent/CN102119031B/zh active Active
-
2017
- 2017-01-23 US US15/412,142 patent/US10357519B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2727392C (en) | 2017-08-22 |
| US20170182100A1 (en) | 2017-06-29 |
| CN103800895A (zh) | 2014-05-21 |
| ES2556630T3 (es) | 2016-01-19 |
| US10357519B2 (en) | 2019-07-23 |
| EP2296672B1 (en) | 2015-09-16 |
| EP2296672A1 (en) | 2011-03-23 |
| BRPI0909866A2 (pt) | 2015-08-11 |
| DK2296672T3 (en) | 2016-01-04 |
| BRPI0909866B1 (pt) | 2021-08-24 |
| PL2296672T3 (pl) | 2016-03-31 |
| PT2296672E (pt) | 2016-01-22 |
| US20110129439A1 (en) | 2011-06-02 |
| US10456425B2 (en) | 2019-10-29 |
| CN102119031A (zh) | 2011-07-06 |
| JP5632833B2 (ja) | 2014-11-26 |
| CN102119031B (zh) | 2014-09-10 |
| JP2011522553A (ja) | 2011-08-04 |
| CA2727392A1 (en) | 2009-12-17 |
| CN103800895B (zh) | 2016-12-28 |
| WO2009150199A1 (en) | 2009-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2296672B1 (en) | Conditioned medium of liver progenitor cells | |
| ES2589311T3 (es) | Poblaciones de células que tienen actividad inmunorreguladora, método de aislamiento y usos | |
| Yan et al. | Injured microenvironment directly guides the differentiation of engrafted Flk-1+ mesenchymal stem cell in lung | |
| CN105008521B (zh) | 调节干细胞的免疫调节作用的方法 | |
| Watanabe et al. | Conditioned mesenchymal stem cells produce pleiotropic gut trophic factors | |
| US10851346B2 (en) | MTOR/STAT3 signal inhibitor-treated mesenchymal stem cell having immunomodulatory activity, and cell therapy composition comprising same, for preventing or treating immune disorders | |
| Vonlaufen et al. | Isolation of quiescent human pancreatic stellate cells: a promising in vitro tool for studies of human pancreatic stellate cell biology | |
| Kim et al. | Anti-arthritic effect of ginsenoside Rb1 on collagen induced arthritis in mice | |
| US20120225130A1 (en) | Compositions, methods, and devices for treating liver disease | |
| CN109219658A (zh) | 用于干细胞培养和治疗的培养基、方法、细胞和分泌的因子 | |
| KR20110038569A (ko) | 인간 유래 성체 줄기세포를 함유하는 항암용 조성물 | |
| US20100129339A1 (en) | Nkt cell-stimulating agent for administration through upper respiratory tract mucous membrane | |
| Furuya et al. | Interleukin-17A plays a pivotal role after partial hepatectomy in mice | |
| CA2540666C (en) | Glucocerebroside treatment of disease | |
| KR101636139B1 (ko) | 면역조절능이 우수한 sRAGE 과발현 간엽줄기세포 및 이를 포함하는 면역질환의 예방 또는 치료용 세포치료제 조성물 | |
| US20190060406A1 (en) | Il-1ra based compositions and treatments | |
| KR20050103259A (ko) | 인터루킨-2 및 모노아세틸디글리세라이드-３을유효성분으로 함유하는 항암제 | |
| TW202206591A (zh) | 體外增殖自然殺手細胞及自然殺手t細胞之方法 | |
| Ohira et al. | Improvement by eicosanoids in cancer cachexia induced by LLC-IL6 transplantation | |
| CN109803681B (zh) | 用于治疗免疫病症的免疫抑制组合物 | |
| Amore et al. | Cytokine mRNA expression by cultured rat mesangial cells after contact with environmental lectins. | |
| Machiyama et al. | TNF receptor associated factor 5 controls oncostatin M-mediated lung inflammation | |
| EP3873503B1 (en) | Treatment of cachexia using fibroblast cells and products thereof | |
| CN106520901A (zh) | 研究三叶青提取物提高人共刺激t细胞增殖的方法 | |
| TWI771561B (zh) | 猴頭菇菌絲體萃取物製備用於改善中樞神經系統髓鞘化之醫藥組合物之用途 |