HRP20211723T1 - Upotreba supstrata za pojačavanje fluorescencije - Google Patents
Upotreba supstrata za pojačavanje fluorescencije Download PDFInfo
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- HRP20211723T1 HRP20211723T1 HRP20211723TT HRP20211723T HRP20211723T1 HR P20211723 T1 HRP20211723 T1 HR P20211723T1 HR P20211723T T HRP20211723T T HR P20211723TT HR P20211723 T HRP20211723 T HR P20211723T HR P20211723 T1 HRP20211723 T1 HR P20211723T1
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- Prior art keywords
- alexa fluor
- analyte
- fluorophore
- substrate
- use according
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- 239000000758 substrate Substances 0.000 title claims 7
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
- 239000012491 analyte Substances 0.000 claims 8
- 239000007787 solid Substances 0.000 claims 7
- 238000000034 method Methods 0.000 claims 6
- OBYNJKLOYWCXEP-UHFFFAOYSA-N 2-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-4-isothiocyanatobenzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(N=C=S)=CC=C1C([O-])=O OBYNJKLOYWCXEP-UHFFFAOYSA-N 0.000 claims 4
- 108010004729 Phycoerythrin Proteins 0.000 claims 4
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 claims 4
- WEJVZSAYICGDCK-UHFFFAOYSA-N Alexa Fluor 430 Chemical compound CC[NH+](CC)CC.CC1(C)C=C(CS([O-])(=O)=O)C2=CC=3C(C(F)(F)F)=CC(=O)OC=3C=C2N1CCCCCC(=O)ON1C(=O)CCC1=O WEJVZSAYICGDCK-UHFFFAOYSA-N 0.000 claims 2
- WHVNXSBKJGAXKU-UHFFFAOYSA-N Alexa Fluor 532 Chemical compound [H+].[H+].CC1(C)C(C)NC(C(=C2OC3=C(C=4C(C(C(C)N=4)(C)C)=CC3=3)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=C2C=3C(C=C1)=CC=C1C(=O)ON1C(=O)CCC1=O WHVNXSBKJGAXKU-UHFFFAOYSA-N 0.000 claims 2
- ZAINTDRBUHCDPZ-UHFFFAOYSA-M Alexa Fluor 546 Chemical compound [H+].[Na+].CC1CC(C)(C)NC(C(=C2OC3=C(C4=NC(C)(C)CC(C)C4=CC3=3)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=C2C=3C(C(=C(Cl)C=1Cl)C(O)=O)=C(Cl)C=1SCC(=O)NCCCCCC(=O)ON1C(=O)CCC1=O ZAINTDRBUHCDPZ-UHFFFAOYSA-M 0.000 claims 2
- IGAZHQIYONOHQN-UHFFFAOYSA-N Alexa Fluor 555 Chemical compound C=12C=CC(=N)C(S(O)(=O)=O)=C2OC2=C(S(O)(=O)=O)C(N)=CC=C2C=1C1=CC=C(C(O)=O)C=C1C(O)=O IGAZHQIYONOHQN-UHFFFAOYSA-N 0.000 claims 2
- 239000012116 Alexa Fluor 680 Substances 0.000 claims 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims 2
- 230000005284 excitation Effects 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 150000002632 lipids Chemical class 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 229910052751 metal Inorganic materials 0.000 claims 2
- 239000002184 metal Substances 0.000 claims 2
- HQCYVSPJIOJEGA-UHFFFAOYSA-N methoxycoumarin Chemical compound C1=CC=C2OC(=O)C(OC)=CC2=C1 HQCYVSPJIOJEGA-UHFFFAOYSA-N 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- 229910052709 silver Inorganic materials 0.000 claims 2
- 239000004332 silver Substances 0.000 claims 2
- QWZHDKGQKYEBKK-UHFFFAOYSA-N 3-aminochromen-2-one Chemical compound C1=CC=C2OC(=O)C(N)=CC2=C1 QWZHDKGQKYEBKK-UHFFFAOYSA-N 0.000 claims 1
- YMZMTOFQCVHHFB-UHFFFAOYSA-N 5-carboxytetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=C(C(O)=O)C=C1C([O-])=O YMZMTOFQCVHHFB-UHFFFAOYSA-N 0.000 claims 1
- 239000012103 Alexa Fluor 488 Substances 0.000 claims 1
- 239000012109 Alexa Fluor 568 Substances 0.000 claims 1
- 239000012110 Alexa Fluor 594 Substances 0.000 claims 1
- 239000012112 Alexa Fluor 633 Substances 0.000 claims 1
- 239000012114 Alexa Fluor 647 Substances 0.000 claims 1
- 239000012115 Alexa Fluor 660 Substances 0.000 claims 1
- 108091023037 Aptamer Proteins 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- -1 Cy3 Substances 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 239000000654 additive Substances 0.000 claims 1
- 229910045601 alloy Inorganic materials 0.000 claims 1
- 239000000956 alloy Substances 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 239000000945 filler Substances 0.000 claims 1
- 238000000799 fluorescence microscopy Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 230000001678 irradiating effect Effects 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 239000000049 pigment Substances 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims 1
- 229920001169 thermoplastic Polymers 0.000 claims 1
- 239000001993 wax Substances 0.000 claims 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N2015/0668—Comparing properties of sample and carrier fluid, e.g. oil in water
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
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- Health & Medical Sciences (AREA)
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- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
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- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Hematology (AREA)
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- Urology & Nephrology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Crystallography & Structural Chemistry (AREA)
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- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Luminescent Compositions (AREA)
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Claims (14)
1. Upotreba supstrata za pojačavanje fluorescencije jedne ili više fluorescentnih molekula, naznačena time što supstrat sadrži čvrsti polimerni nosač koji ima više međusobno razdvojenih udubljenja, te što je čvrsti nosač barem djelomično obložen srebrom ili legurama koje sadrže srebro, pri čemu su udubljenja međusobno udaljena 0,2 µm do 2,5 µm i duboka 0,1 µm do 5 µm, te pri čemu je sloj metala na čvrstom nosaču debeo 10 nm do 60 nm.
2. Upotreba u skladu s patentnim zahtjevom 1, naznačena time što udubljenja u čvrstom nosaču imaju duljinu i širinu, pri čemu je omjer između duljine i širine 2:1 do 1:2, osobito 1:1.
3. Upotreba u skladu s patentnim zahtjevom 1 ili 2, naznačena time što udubljenja u čvrstom nosaču imaju duljinu i širinu, pri čemu su duljina i širina udubljenja 0,1 µm do 2 µm.
4. Upotreba u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačena time što udubljenja imaju uglavnom okrugao oblik.
5. Upotreba u skladu s bilo kojim od patentnih zahtjeva 1 do 4, naznačena time što čvrsti nosač sadrži najmanje jedan materijal kojeg se bira iz skupine koju čine termoplastični polimeri i polikondenzati.
6. Upotreba u skladu s patentnim zahtjevom 5, naznačena time što materijal čvrstog polimernog nosača sadrži organske i/ili anorganske aditive i/ili punila, pri čemu se navedene po mogućnosti bira iz skupine koju čine TiO2, staklo, ugljik, obojeni pigmenti, lipidi i voskovi.
7. Upotreba u skladu s bilo kojim od patentnih zahtjeva 1 do 6, naznačena time što je supstrat dio kapilarne cijevi, mikrotitarske ploče, mikrofluidičkog čipa, analitičke trake, nosača za fluorescentnu mikroskopiju, senzorskog polja ili polja optičkih detektora.
8. Upotreba u skladu s bilo kojim od patentnih zahtjeva 1 do 7, naznačena time što metalna obloga na površini supstrata barem djelomično sadrži molekule za izravno i/ili posredno vezanje fluorescentnih molekula.
9. Postupak određivanja ili kvantificiranja najmanje jednog analita u uzorku, koji se sastoji od ovih koraka:
a) izbornog izravnog ili posrednog obilježavanja najmanje jednog analita najmanje jednim fluoroforom,
b) primjene najmanje jednog obilježenog analita iz koraka a) ili fluorescentnog analita na supstrat u skladu s bilo kojim od patentnih zahtjeva 1 do 8,
c) pobuđivanja najmanje jednog fluorofora ozračivanjem supstrata svjetlom odgovarajuće valne duljine, te
d) mjerenja fluorescencije radi određivanja prisutnosti ili kvantificiranja najmanje jednog analita u uzorku.
10. Postupak u skladu s patentnim zahtjevom 9, naznačen time što najmanje jedan fluorofor ima valnu duljinu pobude u rasponu od 360 do 780 nm, po mogućnosti od 490 do 680 nm.
11. Postupak u skladu s patentnim zahtjevom 9 ili 10, naznačen time što najmanje jedan fluorofor ima valnu duljinu emisije u rasponu od 410 do 800 nm, po mogućnosti od 510 do 710 nm.
12. Postupak u skladu s bilo kojim od patentnih zahtjeva 9 do 11, naznačen time što se najmanje jedan fluorofor bira iz skupine koju čine metoksikumarin, aminokumarin, Cy2, Alexa Fluor 488, fluorescein-izotiocijanat (FITC), Alexa Fluor 430, Alexa Fluor 532, Cy3, Alexa Fluor 555, 5-TAMRA, Alexa Fluor 546, fikoeritrin (PE), tetrametilrodamin-izotiocijanat (TRITC), Cy3.5, rodamin, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 633, Alexa Fluor 647, Cy5, Alexa Fluor 660, Cy5.5, Alexa Fluor 680 i Cy7, po mogućnosti iz skupine koju čine fluorescein-izotiocijanat (FITC), Cy3, fikoeritrin (PE), tetrametilrodamin-izotiocijanat (TRITC), Cy5 i Alexa Fluor 680.
13. Postupak u skladu s bilo kojim od patentnih zahtjeva 9 do 12, naznačen time što se posredno obilježavanje analita najmanje jednim fluoroforom provodi uz pomoć molekule obilježene fluoroforom koja se veže na analit.
14. Postupak u skladu s patentnim zahtjevom 13, naznačen time što se molekulu koja se veže na analit bira iz skupine koju čine protutijela, fragmenti protutijela, po mogućnosti fragmenti Fab, F(ab)’2 ili scFv, nukleinske kiseline, enzimi, lipidi, virusne čestice, aptameri i njihove kombinacije.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ATA50793/2015A AT517746B1 (de) | 2015-09-16 | 2015-09-16 | Substrat |
| PCT/EP2016/071953 WO2017046320A1 (de) | 2015-09-16 | 2016-09-16 | Substrat zur fluoreszenzverstärkung |
| EP16766311.1A EP3350597B1 (de) | 2015-09-16 | 2016-09-16 | Verwendung von substraten zur fluoreszenzverstärkung |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20211723T1 true HRP20211723T1 (hr) | 2022-02-18 |
Family
ID=56936424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20211723TT HRP20211723T1 (hr) | 2015-09-16 | 2016-09-16 | Upotreba supstrata za pojačavanje fluorescencije |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US11262297B2 (hr) |
| EP (1) | EP3350597B1 (hr) |
| JP (1) | JP6968056B2 (hr) |
| CN (1) | CN108351353B (hr) |
| AT (1) | AT517746B1 (hr) |
| CA (1) | CA2998667C (hr) |
| DK (1) | DK3350597T3 (hr) |
| ES (1) | ES2899225T3 (hr) |
| HR (1) | HRP20211723T1 (hr) |
| HU (1) | HUE056568T2 (hr) |
| PL (1) | PL3350597T3 (hr) |
| PT (1) | PT3350597T (hr) |
| RS (1) | RS62645B1 (hr) |
| SI (1) | SI3350597T1 (hr) |
| WO (1) | WO2017046320A1 (hr) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017142745A1 (en) | 2016-02-17 | 2017-08-24 | The Curators Of The University Of Missouri | Fabrication of multilayer nanograting structures |
| CN108872173B (zh) * | 2018-06-29 | 2020-12-15 | 郑州轻工业学院 | 一种荧光增强型适体传感器及其制备方法和应用 |
| AT521641B1 (de) * | 2018-09-12 | 2020-07-15 | Fianostics Gmbh | Verfahren zur Diagnose von Lebererkrankungen |
| CN109487221B (zh) * | 2018-12-12 | 2021-04-02 | 中国科学院合肥物质科学研究院 | 一种Ag-Au-Al-Cr-Cu纳米复合膜表面增强荧光基底及其制备方法 |
| AT522173B1 (de) * | 2019-04-03 | 2020-09-15 | Fianostics Gmbh | Substrat zur Verstärkung der Chemilumineszenz |
| CN110218628B (zh) * | 2019-06-19 | 2021-01-29 | 中国科学院半导体研究所 | 一种数字pcr芯片及其制备方法 |
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| FR2783179B1 (fr) * | 1998-09-16 | 2000-10-06 | Commissariat Energie Atomique | Dispositif d'analyse chimique ou biologique comprenant une pluralite de sites d'analyse sur un support, et son procede de fabrication |
| US7460224B2 (en) * | 2005-12-19 | 2008-12-02 | Opto Trace Technologies, Inc. | Arrays of nano structures for surface-enhanced Raman scattering |
| JP2005274368A (ja) * | 2004-03-25 | 2005-10-06 | Nippon Sheet Glass Co Ltd | バイオチップ用基板 |
| US9012207B2 (en) * | 2005-08-02 | 2015-04-21 | University Of Utah Research Foundation | Biosensors including metallic nanocavities |
| US8535616B2 (en) * | 2005-08-02 | 2013-09-17 | Moxtek, Inc. | Sub-wavelength metallic apertures as light enhancement devices |
| US7988918B2 (en) * | 2007-11-01 | 2011-08-02 | Complete Genomics, Inc. | Structures for enhanced detection of fluorescence |
| EP2257790B1 (en) * | 2008-03-03 | 2016-12-07 | University Of Maryland Baltimore County | Voltage-gated metal-enhanced fluorescence, chemiluminescence or bioluminescence methods and systems |
| JP2009259368A (ja) * | 2008-04-21 | 2009-11-05 | Sony Corp | 光ディスク製造方法、ディスク原盤製造方法、光ディスク |
| US20130065777A1 (en) * | 2009-12-04 | 2013-03-14 | Trustees Of Boston University | Nanostructure biosensors and systems and methods of use thereof |
| JP5544653B2 (ja) * | 2010-02-02 | 2014-07-09 | 独立行政法人産業技術総合研究所 | 抗原抗体反応の検出方法 |
| BR112016014923B1 (pt) * | 2013-12-23 | 2022-11-16 | Illumina, Inc | Arranjo, método para produção de um arranjo e método para detecção de ácidos nucleicos |
| CN104777135B (zh) * | 2015-03-13 | 2018-06-01 | 中山大学 | 一种全波长局域等离子体谐振传感器及其制备方法 |
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| AT517746B1 (de) | 2018-03-15 |
| US11262297B2 (en) | 2022-03-01 |
| PT3350597T (pt) | 2021-11-15 |
| JP6968056B2 (ja) | 2021-11-17 |
| EP3350597A1 (de) | 2018-07-25 |
| SI3350597T1 (sl) | 2022-01-31 |
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