HRP20170069T1 - Polipeptidi dvojnog afiniteta za pročišćavanje - Google Patents
Polipeptidi dvojnog afiniteta za pročišćavanje Download PDFInfo
- Publication number
- HRP20170069T1 HRP20170069T1 HRP20170069TT HRP20170069T HRP20170069T1 HR P20170069 T1 HRP20170069 T1 HR P20170069T1 HR P20170069T T HRP20170069T T HR P20170069TT HR P20170069 T HRP20170069 T HR P20170069T HR P20170069 T1 HRP20170069 T1 HR P20170069T1
- Authority
- HR
- Croatia
- Prior art keywords
- polypeptide
- dual
- target biomolecule
- affinity polypeptide
- affinity
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims 23
- 102000004196 processed proteins & peptides Human genes 0.000 title claims 23
- 229920001184 polypeptide Polymers 0.000 title claims 22
- 230000009977 dual effect Effects 0.000 title claims 6
- 238000000746 purification Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims 17
- 239000003446 ligand Substances 0.000 claims 9
- 239000007787 solid Substances 0.000 claims 8
- 210000004027 cell Anatomy 0.000 claims 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 6
- 238000010494 dissociation reaction Methods 0.000 claims 5
- 230000005593 dissociations Effects 0.000 claims 5
- 230000004927 fusion Effects 0.000 claims 5
- 102000004169 proteins and genes Human genes 0.000 claims 5
- 108090000623 proteins and genes Proteins 0.000 claims 5
- 229960002685 biotin Drugs 0.000 claims 3
- 235000020958 biotin Nutrition 0.000 claims 3
- 239000011616 biotin Substances 0.000 claims 3
- 238000010828 elution Methods 0.000 claims 3
- 108090001008 Avidin Proteins 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 2
- 102000004856 Lectins Human genes 0.000 claims 2
- 108090001090 Lectins Proteins 0.000 claims 2
- 102000019298 Lipocalin Human genes 0.000 claims 2
- 108050006654 Lipocalin Proteins 0.000 claims 2
- 108010090804 Streptavidin Proteins 0.000 claims 2
- 239000001913 cellulose Substances 0.000 claims 2
- 229920002678 cellulose Polymers 0.000 claims 2
- 239000002523 lectin Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 108010087904 neutravidin Proteins 0.000 claims 2
- 239000007790 solid phase Substances 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- XUFXOAAUWZOOIT-SXARVLRPSA-N (2R,3R,4R,5S,6R)-5-[[(2R,3R,4R,5S,6R)-5-[[(2R,3R,4S,5S,6R)-3,4-dihydroxy-6-methyl-5-[[(1S,4R,5S,6S)-4,5,6-trihydroxy-3-(hydroxymethyl)-1-cyclohex-2-enyl]amino]-2-oxanyl]oxy]-3,4-dihydroxy-6-(hydroxymethyl)-2-oxanyl]oxy]-6-(hydroxymethyl)oxane-2,3,4-triol Chemical compound O([C@H]1O[C@H](CO)[C@H]([C@@H]([C@H]1O)O)O[C@H]1O[C@@H]([C@H]([C@H](O)[C@H]1O)N[C@@H]1[C@@H]([C@@H](O)[C@H](O)C(CO)=C1)O)C)[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O XUFXOAAUWZOOIT-SXARVLRPSA-N 0.000 claims 1
- XWNJMSJGJFSGRY-UHFFFAOYSA-N 2-(benzylamino)-3,7-dihydropurin-6-one Chemical class N1C=2N=CNC=2C(=O)N=C1NCC1=CC=CC=C1 XWNJMSJGJFSGRY-UHFFFAOYSA-N 0.000 claims 1
- 229920001817 Agar Polymers 0.000 claims 1
- 229920000936 Agarose Polymers 0.000 claims 1
- 235000002198 Annona diversifolia Nutrition 0.000 claims 1
- 241000282836 Camelus dromedarius Species 0.000 claims 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims 1
- 241000206672 Gelidium Species 0.000 claims 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 241000282842 Lama glama Species 0.000 claims 1
- 239000004952 Polyamide Substances 0.000 claims 1
- 108010085012 Steroid Receptors Proteins 0.000 claims 1
- 229960002632 acarbose Drugs 0.000 claims 1
- XUFXOAAUWZOOIT-UHFFFAOYSA-N acarviostatin I01 Natural products OC1C(O)C(NC2C(C(O)C(O)C(CO)=C2)O)C(C)OC1OC(C(C1O)O)C(CO)OC1OC1C(CO)OC(O)C(O)C1O XUFXOAAUWZOOIT-UHFFFAOYSA-N 0.000 claims 1
- 235000010419 agar Nutrition 0.000 claims 1
- -1 antibody Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 239000001768 carboxy methyl cellulose Substances 0.000 claims 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims 1
- 229920003086 cellulose ether Polymers 0.000 claims 1
- 230000003196 chaotropic effect Effects 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 239000000975 dye Substances 0.000 claims 1
- 239000002532 enzyme inhibitor Substances 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 229920002647 polyamide Polymers 0.000 claims 1
- 229920002451 polyvinyl alcohol Polymers 0.000 claims 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 150000004760 silicates Chemical class 0.000 claims 1
- 102000005969 steroid hormone receptors Human genes 0.000 claims 1
- 150000003431 steroids Chemical class 0.000 claims 1
- 150000003568 thioethers Chemical class 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (17)
1. Postupak za pročišćavanje ciljne biomolekule, koji sadrži korake: (a) dovođenje u kontakt (i) ciljne biomolekule, (ii) polipeptida dvojnog afiniteta, te (iii) krute podloge koja sadrži ligand za hvatanje ili mjesto vezivanja polipeptida dvojnog afiniteta, pri čemu je omjer između konstanti ravnoteže disocijacije polipeptida dvojnog afiniteta, [KD,t / KD,s], barem 10° kod standardnih uvjeta; i (b) obnavljanje ciljne biomolekule pomoću eluiranja, naznačen time da se ciljni afinitetni polipeptid i polipeptid dvojnog afiniteta dovode u kontakt u otopini prije dovođenja u kontakt smjese sa krutom podlogom koja sadrži ligand za hvatanje ili mjesto vezivanja polipeptida dvojnog afiniteta.
2. Postupak prema zahtjevu 1, naznačen time da je kruta podloga odabrana iz skupine koju čine matrice čvrstih faza i čestice.
3. Postupak prema zahtjevu 1, naznačen time da polipeptid dvojnog afiniteta ima konstantu ravnoteže disocijacije, KD,t prema ciljnoj biomolekuli u rasponu od 10-2 do 10-13 M, još specifičnije od 10-4 do 10-13 M, poželjno u rasponu od 10-6 do 10-13 M i konstantu ravnoteže disocijacije, KD,s prema ligandu za hvatanje u rasponu od 10-9 do 10-16 M, poželjno u rasponu od 10-11do 10-16 M.
4. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da je omjer između konstanti ravnoteže disocijacije polipeptida dvojnog afiniteta, [KD,t / KD,s], barem 101, još određenije barem 102, još određenije 103, te čak još određenije barem 104.
5. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da se eluiranje cilja postiže promjenom ili pH, ili ionske jakosti, ili promjenom sadržaja kaotropnih iona u otopini, ili bilo kojom njihovom kombinacijom.
6. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da polipeptid dvojnog afiniteta je fuzijski polipeptid, koji je poželjno odabran iz skupine koju čine protein A, antitijela, fragmenti antitijela, fragmenti proteina A, IgG vezujuće domene dobivene od proteina A, lipokalini, lektini.
7. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da je ligand-vezujući dio polipeptida dvojnog afiniteta odabran iz skupine koju čine avidin, streptavidin, neutravidin, steroidni receptor, antitijelo, fragment antitijela, lipokalini, lektini, amiloglukozidaze, celulozne vezujuće domene.
8. Postupak prema bilo kojem od zahtjeva 6-7, naznačen time da je antitijelo odabrano iz skupine koju čine antitijela lame i deve.
9. Postupak prema zahtjevu 6, naznačen time da je fuzijski polipeptid izrađen fuzijom barem jedne IgG vezujuće domene za protein A ili IgG vezujuće domene dobivene od proteina A i barem jedne biotin vezujuće domene za avidin, streptavidin, ili neutravidin.
10. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da je ligand odabran iz skupine koju čine biotin, akarboza, steroidi, hapten, peptidi epitopa, boje, i inhibitori enzima.
11. Postupak prema zahtjevu 9, naznačen time da ligand za hvatanje koji je vezan na krutu podlogu je biotin i ciljna biomolekula je IgG.
12. Postupak prema bilo kojem od prethodnih zahtjeva, naznačen time da kruta podloga je matrica čvrste faze, poželjno odabrana iz skupine koju čine agar-agar, agaroze, celuloze, celulozni eteri, karboksimetil celuloza, poliamidi, polivinilalkoholi, silikati, te stakla s kontroliranim porama.
13. Postupak prema zahtjevu 6, naznačen time da se fuzijski polipeptid proizvodi kao rekombinantni polipeptid u rekombinantnoj stanici domaćinu.
14. Postupak prema zahtjevu 13, naznačen time da su fuzijski polipeptid i ciljna biomolekula izraženi u istom tipu stanice domaćina.
15. Postupak prema bilo kojem od zahtjeva 13-14, naznačen time da je stanica domaćin odabrana iz skupine koju čine bakterijske stanice, stanice gljiva, stanice sisavaca, stanice biljaka, te stanice insekata.
16. Postupak prema zahtjevu 1, naznačen time da je polipeptid dvojnog afiniteta kemijski kondenziran.
17. Postupak za pročišćavanje ciljne biomolekule, koji sadrži korake: (a) dovođenje u kontakt (i) ciljne biomolekule, (ii) polipeptida dvojnog afiniteta, te (iii) krute podloge koja sadrži ligand za hvatanje, naznačen time da polipeptid dvojnog afiniteta ima konstantu ravnoteže disocijacije, KD,t prema ciljnoj biomolekuli u rasponu od 10-2 do 10-13 M, poželjno od 10-4 do 10-13 M, poželjnije od 10-6 do 10-13 M kod standardnih uvjeta, te pri čemu je vezivanje polipeptida dvojnog afiniteta na ligand za hvatanje na krutoj podlozi osigurano pomoću cijepanja para-supstituiranog benzil gvanina što rezultira s tioeter vezom; i (b) obnavljanje ciljne biomolekule pomoću eluiranja, gdje se ciljni polipeptid i polipeptid dvojnog afiniteta dovode u kontakt u otopini prije dovođenja u kontakt smjese sa krutom podlogom koja sadrži ligand za hvatanje.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07120454 | 2007-11-12 | ||
| PCT/EP2008/065346 WO2009062942A2 (en) | 2007-11-12 | 2008-11-12 | Dual affinity polypeptides for purification |
| EP08850855.1A EP2220107B1 (en) | 2007-11-12 | 2008-11-12 | Dual affinity polypeptides for purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20170069T1 true HRP20170069T1 (hr) | 2017-03-24 |
Family
ID=40568532
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20170069TT HRP20170069T1 (hr) | 2007-11-12 | 2017-01-17 | Polipeptidi dvojnog afiniteta za pročišćavanje |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US20110207916A1 (hr) |
| EP (1) | EP2220107B1 (hr) |
| CN (1) | CN101910194B (hr) |
| AU (1) | AU2008322998B2 (hr) |
| BR (1) | BRPI0820156B8 (hr) |
| CA (1) | CA2705334C (hr) |
| DK (1) | DK2220107T3 (hr) |
| ES (1) | ES2613029T3 (hr) |
| HR (1) | HRP20170069T1 (hr) |
| HU (1) | HUE032930T2 (hr) |
| LT (1) | LT2220107T (hr) |
| PL (1) | PL2220107T3 (hr) |
| PT (1) | PT2220107T (hr) |
| SI (1) | SI2220107T1 (hr) |
| WO (1) | WO2009062942A2 (hr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUE032930T2 (hu) | 2007-11-12 | 2017-11-28 | Chreto Aps | Kettõs affinitású polipeptidek tisztításhoz |
| WO2010128033A2 (en) | 2009-05-07 | 2010-11-11 | Novozymes A/S | Method for purification of target polypeptides |
| WO2011107518A1 (de) | 2010-03-05 | 2011-09-09 | Boehringer Ingelheim International Gmbh | Selektive anreicherung von antikörpern |
| EP2436389A1 (en) * | 2010-10-01 | 2012-04-04 | Nestec S.A. | Milk-based protein hydrolysates and infant formulae and nutritional compositions made thereof |
| CA2809916A1 (en) * | 2010-10-01 | 2012-04-05 | Novozymes A/S | Polypeptides having endopeptidase activity and polynucleotides encoding same |
| EP3299388A1 (en) * | 2012-03-28 | 2018-03-28 | The Board of Regents of The University of Texas System | Tgf type ii-type iii receptor fusions |
| US10755210B2 (en) | 2014-08-15 | 2020-08-25 | Ecolab Usa Inc. | CIP wash summary and library |
| US10315235B2 (en) * | 2014-08-15 | 2019-06-11 | Ecolab Usa Inc. | CIP wash comparison and simulation |
| EP3212787B1 (en) * | 2014-10-28 | 2019-07-10 | Merck Patent GmbH | Methods for non-covalent fc-domain-containing protein display on the surface of cells and methods of screening thereof |
| EP3805384A1 (en) * | 2015-05-22 | 2021-04-14 | DuPont Nutrition Biosciences ApS | Aldc production methods |
| CN108191956B (zh) * | 2017-12-25 | 2020-10-30 | 浙江大学 | 组合型配基、组合型仿生层析介质及其制备方法和应用 |
| CN108440666B (zh) * | 2018-03-07 | 2021-03-23 | 深圳市伯劳特生物制品有限公司 | 一种生物素化的insulin抗原及其生物素化工艺 |
| EP4134158B1 (en) | 2018-12-17 | 2024-05-15 | Solventum Intellectual Properties Company | Ligand linker substrate |
| JP7249432B2 (ja) | 2019-03-29 | 2023-03-30 | エフ． ホフマン－ラ ロシュ アーゲー | 多価分子の機能分析のための、ｓｐｒをベースとする結合アッセイ |
| CN114591407B (zh) * | 2021-12-21 | 2023-07-04 | 百林科(兰州)新材料有限公司 | 耐碱蛋白a变体及其应用 |
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| CN116068206A (zh) * | 2023-03-15 | 2023-05-05 | 安徽惠邦生物工程有限公司 | 一种髓鞘碱性蛋白检测试剂盒及其检测方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE243754T1 (de) * | 1987-05-21 | 2003-07-15 | Micromet Ag | Multifunktionelle proteine mit vorbestimmter zielsetzung |
| US5169936A (en) | 1989-04-14 | 1992-12-08 | Biogen, Inc. | Protein purification on immobilized metal affinity resins effected by elution using a weak ligand |
| US5328985A (en) * | 1991-07-12 | 1994-07-12 | The Regents Of The University Of California | Recombinant streptavidin-protein chimeras useful for conjugation of molecules in the immune system |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| WO1997009068A2 (en) | 1995-09-01 | 1997-03-13 | University Of Washington | Interactive molecular conjugates |
| US6497881B1 (en) | 1995-11-30 | 2002-12-24 | New York University | High efficiency tissue specific compound delivery system using streptavidin-protein a fusion protein |
| US6753189B1 (en) * | 1998-06-04 | 2004-06-22 | Mizuho Medy Co., Ltd. | Detection apparatus and method for the same |
| US6831160B1 (en) | 2000-01-24 | 2004-12-14 | The Regents Of The University Of California | Method of affinity purifying proteins using modified bis-arsenical fluorescein |
| MXPA02012438A (es) * | 2000-06-16 | 2004-09-06 | Dept Of Radiation Oncology Uni | Columnas extracorporeas para uso repetido, cargadas con conjugados de ligando-dibiotina. |
| EP1529844B2 (en) | 2003-07-21 | 2018-11-07 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Complex formation for the stabilisation and purification of proteins of interest |
| US7956165B2 (en) * | 2003-07-24 | 2011-06-07 | Affisink Biotechnology Ltd. | Compositions and methods for purifying and crystallizing molecules of interest |
| FI20031663A0 (fi) | 2003-11-14 | 2003-11-14 | Jyvaeskylaen Yliopisto | Avidiinin mutantteja |
| WO2006059904A1 (en) * | 2004-12-02 | 2006-06-08 | Unilever N.V. | Method for affinity purification |
| US20070218535A1 (en) | 2005-11-28 | 2007-09-20 | Xinli Lin | Methods for production of recombinant alpha1-antitrypsin |
| EP1989548A2 (en) * | 2006-02-08 | 2008-11-12 | Promega Corporation | Compositions and methods for capturing and analyzing cross-linked biomolecules |
| GB2454153A (en) * | 2006-09-05 | 2009-04-29 | Innovative Purification Techno | Affinity separation methods and systems |
| CN101054594A (zh) * | 2007-03-30 | 2007-10-17 | 南开大学 | 双功能融合蛋白CBD－ProA载体及其制备的免疫磁性微球和应用 |
| HUE032930T2 (hu) | 2007-11-12 | 2017-11-28 | Chreto Aps | Kettõs affinitású polipeptidek tisztításhoz |
-
2008
- 2008-11-12 HU HUE08850855A patent/HUE032930T2/hu unknown
- 2008-11-12 DK DK08850855.1T patent/DK2220107T3/en active
- 2008-11-12 PL PL08850855T patent/PL2220107T3/pl unknown
- 2008-11-12 EP EP08850855.1A patent/EP2220107B1/en active Active
- 2008-11-12 BR BRPI0820156A patent/BRPI0820156B8/pt active IP Right Grant
- 2008-11-12 PT PT88508551T patent/PT2220107T/pt unknown
- 2008-11-12 CA CA2705334A patent/CA2705334C/en active Active
- 2008-11-12 AU AU2008322998A patent/AU2008322998B2/en active Active
- 2008-11-12 ES ES08850855.1T patent/ES2613029T3/es active Active
- 2008-11-12 CN CN200880124535.2A patent/CN101910194B/zh active Active
- 2008-11-12 LT LTEP08850855.1T patent/LT2220107T/lt unknown
- 2008-11-12 WO PCT/EP2008/065346 patent/WO2009062942A2/en active Application Filing
- 2008-11-12 US US12/740,940 patent/US20110207916A1/en not_active Abandoned
- 2008-11-12 SI SI200831751A patent/SI2220107T1/sl unknown
-
2013
- 2013-11-21 US US14/086,621 patent/US9376463B2/en active Active
-
2017
- 2017-01-17 HR HRP20170069TT patent/HRP20170069T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009062942A2 (en) | 2009-05-22 |
| DK2220107T3 (en) | 2017-02-13 |
| BRPI0820156B8 (pt) | 2021-05-25 |
| WO2009062942A3 (en) | 2010-02-18 |
| US20140081001A1 (en) | 2014-03-20 |
| US20110207916A1 (en) | 2011-08-25 |
| PL2220107T3 (pl) | 2017-05-31 |
| CA2705334C (en) | 2018-04-17 |
| EP2220107A2 (en) | 2010-08-25 |
| SI2220107T1 (sl) | 2017-02-28 |
| EP2220107B1 (en) | 2016-11-02 |
| CN101910194A (zh) | 2010-12-08 |
| US9376463B2 (en) | 2016-06-28 |
| HUE032930T2 (hu) | 2017-11-28 |
| PT2220107T (pt) | 2017-02-08 |
| CA2705334A1 (en) | 2009-05-22 |
| LT2220107T (lt) | 2017-04-10 |
| BRPI0820156A2 (pt) | 2015-10-20 |
| AU2008322998A1 (en) | 2009-05-22 |
| ES2613029T3 (es) | 2017-05-22 |
| CN101910194B (zh) | 2017-03-08 |
| BRPI0820156B1 (pt) | 2021-01-12 |
| AU2008322998B2 (en) | 2013-10-03 |
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