HRP20110354T1 - Pripravak za tvorbu kosti koji se sastoji od smjese osteoblasta i bio-matrice i postupak za njegovo dobivanje - Google Patents
Pripravak za tvorbu kosti koji se sastoji od smjese osteoblasta i bio-matrice i postupak za njegovo dobivanje Download PDFInfo
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- HRP20110354T1 HRP20110354T1 HR20110354T HRP20110354T HRP20110354T1 HR P20110354 T1 HRP20110354 T1 HR P20110354T1 HR 20110354 T HR20110354 T HR 20110354T HR P20110354 T HRP20110354 T HR P20110354T HR P20110354 T1 HRP20110354 T1 HR P20110354T1
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- Prior art keywords
- osteoblast
- preparation
- bio
- osteoblasts
- matrix
- Prior art date
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- 210000000963 osteoblast Anatomy 0.000 title claims abstract 22
- 230000011164 ossification Effects 0.000 title claims abstract 10
- 239000011159 matrix material Substances 0.000 title claims abstract 8
- 239000000203 mixture Substances 0.000 title claims abstract 7
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 239000000725 suspension Substances 0.000 claims abstract 9
- 210000000988 bone and bone Anatomy 0.000 claims abstract 6
- 239000003814 drug Substances 0.000 claims abstract 3
- 238000000034 method Methods 0.000 claims abstract 3
- 229940124597 therapeutic agent Drugs 0.000 claims abstract 3
- 238000002360 preparation method Methods 0.000 claims 9
- 108090000190 Thrombin Proteins 0.000 claims 5
- 229960004072 thrombin Drugs 0.000 claims 5
- 108010049003 Fibrinogen Proteins 0.000 claims 4
- 102000008946 Fibrinogen Human genes 0.000 claims 4
- 229940012952 fibrinogen Drugs 0.000 claims 4
- 239000007758 minimum essential medium Substances 0.000 claims 4
- 102000008186 Collagen Human genes 0.000 claims 3
- 108010035532 Collagen Proteins 0.000 claims 3
- 239000003795 chemical substances by application Substances 0.000 claims 3
- 230000015271 coagulation Effects 0.000 claims 3
- 238000005345 coagulation Methods 0.000 claims 3
- 229920001436 collagen Polymers 0.000 claims 3
- 239000000243 solution Substances 0.000 claims 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 2
- 239000011575 calcium Substances 0.000 claims 2
- 229910052791 calcium Inorganic materials 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 2
- 150000002500 ions Chemical class 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 239000011707 mineral Substances 0.000 claims 2
- 239000011259 mixed solution Substances 0.000 claims 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 2
- -1 phosphate (PO43-) ions Chemical class 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
- 238000006386 neutralization reaction Methods 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 abstract 2
- 238000002347 injection Methods 0.000 abstract 2
- 239000007924 injection Substances 0.000 abstract 2
- 206010052779 Transplant rejections Diseases 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000009772 tissue formation Effects 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/36—Blood coagulation or fibrinolysis factors
- A61K38/363—Fibrinogen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
- A61K38/482—Serine endopeptidases (3.4.21)
- A61K38/4833—Thrombin (3.4.21.5)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3847—Bones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/56—Fibrin; Thrombin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
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- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- Transplantation (AREA)
- Rheumatology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Physical Education & Sports Medicine (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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Abstract
Pripravak za tvorbu kosti koji je dobiven postupkom za pripremu pripravka za tvorbu kosti korištenjem smjese osteoblasta i bio-matrice, naznačen time da se sastoji od: uzgoja/izrastanja izoliranih osteoblasta i njihovih prekursorskih stanica u alfa-MEM (minimalni esencijalni medij, iz alfa izmjene koštanog tkiva) za pripremu suspenzije osteoblasta; imiješanja rezultirajuće suspenzije osteoblasta sa bio-matricom za pripremu osteoblastnog terapijskog sredstva, pri čemu je bio-matrica mješavina kolagena i hidroksiapatita, pri čemu je kolagen dodan u iznosu od 67 µg/mL do 20 mg/mL u suspenziju osteoblasta, a hidroksiapatit se dodaje se u iznosu od 30 µg/mL do 3,4 mg/mL u suspenziju osteoblasta. Patent sadrži još 4 patentna zahtjeva.
Claims (6)
1. Pripravak za tvorbu kosti koji je dobiven postupkom za pripremu pripravka za tvorbu kosti korištenjem smjese osteoblasta i bio-matrice, naznačen time da se sastoji od:
uzgoja/izrastanja izoliranih osteoblasta i njihovih prekursorskih stanica u alfa-MEM (minimalni esencijalni medij, iz alfa izmjene koštanog tkiva) za pripremu suspenzije osteoblasta; i
miješanja rezultirajuće suspenzije osteoblasta sa bio-matricom za pripremu osteoblastnog terapijskog sredstva,
pri čemu je bio-matrica mješavina kolagena i hidroksiapatita,
pri čemu je kolagen dodan u iznosu od 67 µg/mL do 20 mg/mL u suspenziju osteoblasta, a hidroksiapatit se dodaje se u iznosu od 30 µg/mL do 3,4 mg/mL u suspenziju osteoblasta.
2. Pripravak za tvorbu kosti prema zahtjevu 1, naznačen time da korak pripreme terapijskog sredstva nadalje uključuje miješanje otopine izmiješanih osteoblasta, u kojoj je izmiješana bio-matrica u suspenziju osteoblasta, sa koagulacijskim sredstvom.
3. Pripravak za tvorbu kosti prema zahtjevu 2, naznačen time da korak miješanja uključuje:
4. miješanje izmiješane otopine osteoblasta sa 10 do 100 IU/mL trombina kao koagulacijskog sredstva; te miješanje nastale izmiješane otopine koja sadrži u njoj izmiješan trombin sa 20 do 100 mg/mL fibrinogena kao koagulacijskog sredstva.
5. Pripravak za tvorbu kosti prema zahtjevu 1, naznačen time da je kolagen neutraliziran do neutralnog pH uz dodavanje neutralizacijske otopine prije miješanja sa suspenzijom osteoblasta.
6. Pripravak za tvorbu kosti prema zahtjevu 3, naznačen time da
prije dodavanja u smjesu osteoblasta i bio-matrice, liofilizirani trombin kao trombin je otopljen u tekućini alfa-MEM u koju su dodani fosfatni (PO43-) ioni kao mineralna komponenta kosti, te se dodaje u dvostrukoj količini od količine trombina sadržanog u mediju kulture u koju se dodaju fosfatni (PO43-) ioni; te
prije dodavanja u rezultirajuću otopinu smjese osteoblasta, liofilizirani fibrinogen kao fibrinogen je otopljen u tekućini alfa-MEM u koju su dodani kalcijevi (Ca2+) ioni kao mineralna komponenta kosti, te se dodaje u dvostrukoj količini od količine fibrinogena sadržanog u mediju kulture u koju se dodaju kalcijevi (Ca2+) ioni.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020050050447A KR100751690B1 (ko) | 2005-06-13 | 2005-06-13 | 조골 세포와 생체 기질 성분의 혼합물을 이용한 골 생성용조성물 및 그 제조방법 |
| PCT/KR2005/002006 WO2006135123A1 (en) | 2005-06-13 | 2005-06-27 | Bone formation composition composed of mixture of osteoblast and bio-matrix and its manufacturing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20110354T1 true HRP20110354T1 (hr) | 2011-06-30 |
Family
ID=37532458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HR20110354T HRP20110354T1 (hr) | 2005-06-13 | 2011-05-12 | Pripravak za tvorbu kosti koji se sastoji od smjese osteoblasta i bio-matrice i postupak za njegovo dobivanje |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20090311220A1 (hr) |
| EP (1) | EP1896044B1 (hr) |
| JP (1) | JP2008542362A (hr) |
| KR (1) | KR100751690B1 (hr) |
| CN (1) | CN101227915A (hr) |
| AT (1) | ATE503005T1 (hr) |
| CY (1) | CY1111582T1 (hr) |
| DE (1) | DE602005027129D1 (hr) |
| DK (1) | DK1896044T3 (hr) |
| ES (1) | ES2361754T3 (hr) |
| HR (1) | HRP20110354T1 (hr) |
| PL (1) | PL1896044T3 (hr) |
| PT (1) | PT1896044E (hr) |
| RS (1) | RS51942B (hr) |
| SI (1) | SI1896044T1 (hr) |
| WO (1) | WO2006135123A1 (hr) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008212265A (ja) * | 2007-03-01 | 2008-09-18 | Niigata Univ | 培養人工骨及びその作製方法 |
| KR20100007180A (ko) * | 2008-07-11 | 2010-01-22 | 세원셀론텍(주) | 뼈 재생용 콜라겐 젤 조성물의 제조방법 |
| EP2575441A1 (en) * | 2010-05-28 | 2013-04-10 | Garnet BioTherapeutics, Inc | Compositions and methods of using living and non-living bioactive devices with components derived from self-renewing colony forming cells cultured and expanded in vitro |
| KR102648746B1 (ko) * | 2020-05-13 | 2024-03-18 | 한국기초과학지원연구원 | 골 조직 모사체 및 이의 제조방법 |
| CN112708592A (zh) * | 2021-01-12 | 2021-04-27 | 赵彬 | 一种成骨细胞蛋白质的3d结构 |
| WO2024092300A1 (en) * | 2022-11-04 | 2024-05-10 | Royal Melbourne Institute Of Technology | Method of differentiating stem cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US4837379A (en) * | 1988-06-02 | 1989-06-06 | Organogenesis Inc. | Fibrin-collagen tissue equivalents and methods for preparation thereof |
| JP2984176B2 (ja) * | 1993-12-30 | 1999-11-29 | 新田ゼラチン株式会社 | 骨髄細胞の培養方法、培養用混合物および硬組織欠損部への移植用材料 |
| JPH07213598A (ja) * | 1994-02-07 | 1995-08-15 | Terumo Corp | 骨セメント |
| US5942496A (en) * | 1994-02-18 | 1999-08-24 | The Regent Of The University Of Michigan | Methods and compositions for multiple gene transfer into bone cells |
| JPH07242564A (ja) * | 1994-03-02 | 1995-09-19 | Sagami Chem Res Center | 骨形成促進剤 |
| JPH08112341A (ja) * | 1994-10-12 | 1996-05-07 | Japan Steel Works Ltd:The | 骨充填材およびその製造方法 |
| US6599515B1 (en) * | 1995-01-16 | 2003-07-29 | Baxter International Inc. | Fibrin porous structure |
| JPH10243996A (ja) * | 1997-03-07 | 1998-09-14 | Kagaku Gijutsu Shinko Jigyodan | 硬組織石灰化促進用生体材料 |
| US20030180263A1 (en) * | 2002-02-21 | 2003-09-25 | Peter Geistlich | Resorbable extracellular matrix for reconstruction of bone |
| EP1100558A1 (en) * | 1998-07-24 | 2001-05-23 | Pharmacal Biotechnologies, Inc. | Osseous tissue reconstruction system and method |
| DE19949290A1 (de) * | 1999-10-12 | 2001-04-26 | Albrecht Bettermann | Partikuläres Konstrukt zur Verwendung in der Transplantationsmedizin |
| JP2001129073A (ja) * | 1999-11-02 | 2001-05-15 | Olympus Optical Co Ltd | 骨補填材および骨補填材注入具 |
| DE19956503A1 (de) * | 1999-11-24 | 2001-06-21 | Universitaetsklinikum Freiburg | Spritzbares Knochenersatzmaterial |
| IL144446A0 (en) * | 2001-07-19 | 2002-05-23 | Prochon Biotech Ltd | Plasma protein matrices and methods for their preparation |
| JP2003081869A (ja) * | 2001-09-17 | 2003-03-19 | Asahi Kasei Corp | 骨髄細胞または骨芽細胞移植医療用補助剤 |
| KR100522457B1 (ko) * | 2002-04-13 | 2005-10-18 | 주식회사 셀론텍 | 뼈세포의 배양방법 및 조성물 |
| US20070077236A1 (en) * | 2003-06-12 | 2007-04-05 | Interface Biotech A/S | Method for cell implantation |
| US7316822B2 (en) * | 2003-11-26 | 2008-01-08 | Ethicon, Inc. | Conformable tissue repair implant capable of injection delivery |
| KR100531922B1 (ko) * | 2003-12-23 | 2005-11-29 | 주식회사 셀론텍 | 연골치료제 조성물 및 그 사용방법 |
-
2005
- 2005-06-13 KR KR1020050050447A patent/KR100751690B1/ko active IP Right Grant
- 2005-06-27 SI SI200531298T patent/SI1896044T1/sl unknown
- 2005-06-27 JP JP2008514538A patent/JP2008542362A/ja active Pending
- 2005-06-27 PT PT05765896T patent/PT1896044E/pt unknown
- 2005-06-27 EP EP05765896A patent/EP1896044B1/en active Active
- 2005-06-27 ES ES05765896T patent/ES2361754T3/es active Active
- 2005-06-27 PL PL05765896T patent/PL1896044T3/pl unknown
- 2005-06-27 DE DE602005027129T patent/DE602005027129D1/de active Active
- 2005-06-27 DK DK05765896.5T patent/DK1896044T3/da active
- 2005-06-27 WO PCT/KR2005/002006 patent/WO2006135123A1/en active Application Filing
- 2005-06-27 US US11/921,217 patent/US20090311220A1/en not_active Abandoned
- 2005-06-27 AT AT05765896T patent/ATE503005T1/de active
- 2005-06-27 CN CNA2005800499663A patent/CN101227915A/zh active Pending
- 2005-06-27 RS RS20110204A patent/RS51942B/en unknown
-
2011
- 2011-05-12 HR HR20110354T patent/HRP20110354T1/hr unknown
- 2011-06-22 CY CY20111100592T patent/CY1111582T1/el unknown
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| Publication number | Publication date |
|---|---|
| CY1111582T1 (el) | 2015-10-07 |
| PT1896044E (pt) | 2011-05-31 |
| ES2361754T3 (es) | 2011-06-21 |
| JP2008542362A (ja) | 2008-11-27 |
| DE602005027129D1 (de) | 2011-05-05 |
| EP1896044A4 (en) | 2009-07-15 |
| EP1896044B1 (en) | 2011-03-23 |
| PL1896044T3 (pl) | 2011-08-31 |
| CN101227915A (zh) | 2008-07-23 |
| US20090311220A1 (en) | 2009-12-17 |
| ATE503005T1 (de) | 2011-04-15 |
| KR20060129792A (ko) | 2006-12-18 |
| WO2006135123A1 (en) | 2006-12-21 |
| EP1896044A1 (en) | 2008-03-12 |
| KR100751690B1 (ko) | 2007-08-23 |
| SI1896044T1 (sl) | 2011-07-29 |
| DK1896044T3 (da) | 2011-06-20 |
| RS51942B (en) | 2012-02-29 |
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