HRP20110391T1 - Pripravak osteoblasta od poluočvrsnulog izmješanog fibrina za aglutinaciju prijeloma kosti i njegov postupak proizvodnje - Google Patents
Pripravak osteoblasta od poluočvrsnulog izmješanog fibrina za aglutinaciju prijeloma kosti i njegov postupak proizvodnje Download PDFInfo
- Publication number
- HRP20110391T1 HRP20110391T1 HR20110391T HRP20110391T HRP20110391T1 HR P20110391 T1 HRP20110391 T1 HR P20110391T1 HR 20110391 T HR20110391 T HR 20110391T HR P20110391 T HRP20110391 T HR P20110391T HR P20110391 T1 HRP20110391 T1 HR P20110391T1
- Authority
- HR
- Croatia
- Prior art keywords
- osteoblast
- semi
- bone
- fibrin
- composition
- Prior art date
Links
- 210000000963 osteoblast Anatomy 0.000 title claims abstract 17
- 102000009123 Fibrin Human genes 0.000 title claims abstract 6
- 108010073385 Fibrin Proteins 0.000 title claims abstract 6
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 title claims abstract 6
- 229950003499 fibrin Drugs 0.000 title claims abstract 6
- 239000000203 mixture Substances 0.000 title claims abstract 6
- 208000010392 Bone Fractures Diseases 0.000 title claims abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 230000004520 agglutination Effects 0.000 title 1
- 210000000988 bone and bone Anatomy 0.000 claims abstract 4
- 239000000725 suspension Substances 0.000 claims abstract 4
- 108010039209 Blood Coagulation Factors Proteins 0.000 claims abstract 3
- 102000015081 Blood Coagulation Factors Human genes 0.000 claims abstract 3
- 239000003114 blood coagulation factor Substances 0.000 claims abstract 3
- 239000003814 drug Substances 0.000 claims abstract 3
- 229940124597 therapeutic agent Drugs 0.000 claims abstract 3
- 238000000034 method Methods 0.000 claims abstract 2
- 230000004048 modification Effects 0.000 claims abstract 2
- 238000012986 modification Methods 0.000 claims abstract 2
- 108090000190 Thrombin Proteins 0.000 claims 4
- 238000002360 preparation method Methods 0.000 claims 4
- 229960004072 thrombin Drugs 0.000 claims 4
- 108010049003 Fibrinogen Proteins 0.000 claims 3
- 102000008946 Fibrinogen Human genes 0.000 claims 3
- 229940012952 fibrinogen Drugs 0.000 claims 3
- 239000007788 liquid Substances 0.000 claims 2
- 239000007787 solid Substances 0.000 abstract 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 abstract 2
- 238000002347 injection Methods 0.000 abstract 2
- 239000007924 injection Substances 0.000 abstract 2
- 239000007758 minimum essential medium Substances 0.000 abstract 2
- 206010052779 Transplant rejections Diseases 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000002062 proliferating effect Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/36—Blood coagulation or fibrinolysis factors
- A61K38/363—Fibrinogen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
- A61K38/482—Serine endopeptidases (3.4.21)
- A61K38/4833—Thrombin (3.4.21.5)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Rheumatology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Hematology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
Polu-očvrsnuti pripravak osteoblasta koji sadrži fibrin za liječenje prijeloma kosti, naznačen time da je pripravak dobiven postupkom koji se sastoji od: uzgoja/izrastanja izoliranih osteoblasta iz koštanog tkiva u alfa modifikaciji minimalnog esencijalnog medija (alfa-MEM) za pripremu suspenzije osteoblasta, imiješanja rezultirajuće suspenzije osteoblasta sa koagulacijskim čimbenikom za pripremu osteoblastnog terapijskog sredstva, pri čemu je navedeni koagulacijski čimbenik mješavina trombina i fibrinogena, tepri čemu koraci pripreme terapijskog sredstva uključuju: otapanje liofiliziranog trombina u tekućem alfa-MEM i nakon toga miješanje 1 do 100 IU/mL otopljenog trombina sa izmiješanom otopinom osteoblasta, teotapanje liofiliziranog fibrinogena u tekućem alfa-MEM i nakon toga miješanje 20 mg/mL do 100 mg/mL otopljenog fibrinogena sa izmiješanom otopinom osteoblasta koja sadrži u njoj izmiješan trombin.
Claims (1)
1. Polu-očvrsnuti pripravak osteoblasta koji sadrži fibrin za liječenje prijeloma kosti, naznačen time da je pripravak dobiven postupkom koji se sastoji od:
uzgoja/izrastanja izoliranih osteoblasta iz koštanog tkiva u alfa modifikaciji minimalnog esencijalnog medija (alfa-MEM) za pripremu suspenzije osteoblasta, i
miješanja rezultirajuće suspenzije osteoblasta sa koagulacijskim čimbenikom za pripremu osteoblastnog terapijskog sredstva, pri čemu je navedeni koagulacijski čimbenik mješavina trombina i fibrinogena, te
pri čemu koraci pripreme terapijskog sredstva uključuju:
otapanje liofiliziranog trombina u tekućem alfa-MEM i nakon toga miješanje 1 do 100 IU/mL otopljenog trombina sa izmiješanom otopinom osteoblasta, te
otapanje liofiliziranog fibrinogena u tekućem alfa-MEM i nakon toga miješanje 20 mg/mL do 100 mg/mL otopljenog fibrinogena sa izmiješanom otopinom osteoblasta koja sadrži u njoj izmiješan trombin.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020050050450A KR100702250B1 (ko) | 2005-06-13 | 2005-06-13 | 피브린 혼합형 골절 유합용 반고형성 뼈세포 조성물 및이의 제조방법 |
| PCT/KR2005/002531 WO2006135126A1 (en) | 2005-06-13 | 2005-08-03 | Osteoblast composition of semi-solidified mixed fibrin for bone fracture agglutination and its manufacturing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20110391T1 true HRP20110391T1 (hr) | 2011-06-30 |
Family
ID=37532459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HR20110391T HRP20110391T1 (hr) | 2005-06-13 | 2011-05-24 | Pripravak osteoblasta od poluočvrsnulog izmješanog fibrina za aglutinaciju prijeloma kosti i njegov postupak proizvodnje |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US20080213229A1 (hr) |
| EP (1) | EP1890713B1 (hr) |
| JP (1) | JP2008532723A (hr) |
| KR (1) | KR100702250B1 (hr) |
| CN (1) | CN101247814A (hr) |
| AT (1) | ATE503486T1 (hr) |
| BR (1) | BRPI0520049B8 (hr) |
| CY (1) | CY1111795T1 (hr) |
| DE (1) | DE602005027260D1 (hr) |
| DK (1) | DK1890713T3 (hr) |
| ES (1) | ES2362430T3 (hr) |
| HR (1) | HRP20110391T1 (hr) |
| MX (1) | MX2007012041A (hr) |
| PL (1) | PL1890713T3 (hr) |
| PT (1) | PT1890713E (hr) |
| RS (1) | RS51724B (hr) |
| SG (1) | SG162750A1 (hr) |
| SI (1) | SI1890713T1 (hr) |
| WO (1) | WO2006135126A1 (hr) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101037002B1 (ko) * | 2008-09-30 | 2011-05-25 | 세원셀론텍(주) | 연골세포 조기배양을 위한 연골세포 특이적 배양방법 |
| KR100932945B1 (ko) | 2009-01-16 | 2009-12-21 | 원광대학교산학협력단 | 골 분말과 피브린 글루로 이루어진 고형의 스캐폴드 |
| KR100932948B1 (ko) * | 2009-01-16 | 2009-12-21 | 원광대학교산학협력단 | Ｂｍｐ-2를 함유하는 골 분말과 피브린 글루로 이루어진 고형의 스캐폴드 |
| WO2020161747A1 (en) * | 2019-02-08 | 2020-08-13 | Regrow Biosciences Private Limited | Osteoblast cell-mixture, and implementations thereof |
| CN113827778B (zh) * | 2021-11-03 | 2022-10-21 | 浙江赛灵特医药科技有限公司 | 一种注射式骨修复剂及其应用 |
| CN113975468A (zh) * | 2021-11-11 | 2022-01-28 | 成都中科娥皇健康咨询有限公司 | 一种骨膜组织老化修复剂及其制备工艺 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5972703A (en) * | 1994-08-12 | 1999-10-26 | The Regents Of The University Of Michigan | Bone precursor cells: compositions and methods |
| US6599515B1 (en) * | 1995-01-16 | 2003-07-29 | Baxter International Inc. | Fibrin porous structure |
| DE19949290A1 (de) * | 1999-10-12 | 2001-04-26 | Albrecht Bettermann | Partikuläres Konstrukt zur Verwendung in der Transplantationsmedizin |
| DE19956503A1 (de) * | 1999-11-24 | 2001-06-21 | Universitaetsklinikum Freiburg | Spritzbares Knochenersatzmaterial |
| JP2003525696A (ja) * | 2000-03-03 | 2003-09-02 | スミス アンド ネフュー インコーポレーテッド | 造形粒子および骨欠損用組成物および粒子を作る方法 |
| IL144446A0 (en) * | 2001-07-19 | 2002-05-23 | Prochon Biotech Ltd | Plasma protein matrices and methods for their preparation |
| KR100522457B1 (ko) * | 2002-04-13 | 2005-10-18 | 주식회사 셀론텍 | 뼈세포의 배양방법 및 조성물 |
| EP1635887A2 (en) * | 2003-06-12 | 2006-03-22 | Interface Biotech A/S | A method for cell implantation |
| US7316822B2 (en) * | 2003-11-26 | 2008-01-08 | Ethicon, Inc. | Conformable tissue repair implant capable of injection delivery |
-
2005
- 2005-06-13 KR KR1020050050450A patent/KR100702250B1/ko active IP Right Grant
- 2005-08-03 SI SI200531313T patent/SI1890713T1/sl unknown
- 2005-08-03 US US11/885,943 patent/US20080213229A1/en not_active Abandoned
- 2005-08-03 ES ES05774219T patent/ES2362430T3/es active Active
- 2005-08-03 RS RS20110212A patent/RS51724B/en unknown
- 2005-08-03 PT PT05774219T patent/PT1890713E/pt unknown
- 2005-08-03 DK DK05774219.9T patent/DK1890713T3/da active
- 2005-08-03 JP JP2008502891A patent/JP2008532723A/ja active Pending
- 2005-08-03 SG SG201003922-0A patent/SG162750A1/en unknown
- 2005-08-03 PL PL05774219T patent/PL1890713T3/pl unknown
- 2005-08-03 BR BRPI0520049A patent/BRPI0520049B8/pt not_active IP Right Cessation
- 2005-08-03 CN CNA2005800492946A patent/CN101247814A/zh active Pending
- 2005-08-03 AT AT05774219T patent/ATE503486T1/de active
- 2005-08-03 MX MX2007012041A patent/MX2007012041A/es active IP Right Grant
- 2005-08-03 EP EP05774219A patent/EP1890713B1/en active Active
- 2005-08-03 WO PCT/KR2005/002531 patent/WO2006135126A1/en active Application Filing
- 2005-08-03 DE DE602005027260T patent/DE602005027260D1/de active Active
-
2011
- 2011-05-24 HR HR20110391T patent/HRP20110391T1/hr unknown
- 2011-06-28 CY CY20111100616T patent/CY1111795T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE602005027260D1 (de) | 2011-05-12 |
| BRPI0520049B8 (pt) | 2021-05-25 |
| BRPI0520049B1 (pt) | 2020-12-22 |
| WO2006135126A1 (en) | 2006-12-21 |
| KR20060129795A (ko) | 2006-12-18 |
| EP1890713A4 (en) | 2009-07-08 |
| PT1890713E (pt) | 2011-06-09 |
| BRPI0520049A2 (pt) | 2009-04-14 |
| SG162750A1 (en) | 2010-07-29 |
| KR100702250B1 (ko) | 2007-04-03 |
| EP1890713B1 (en) | 2011-03-30 |
| US20080213229A1 (en) | 2008-09-04 |
| JP2008532723A (ja) | 2008-08-21 |
| CN101247814A (zh) | 2008-08-20 |
| EP1890713A1 (en) | 2008-02-27 |
| MX2007012041A (es) | 2008-03-10 |
| ATE503486T1 (de) | 2011-04-15 |
| SI1890713T1 (sl) | 2011-07-29 |
| DK1890713T3 (da) | 2011-06-27 |
| CY1111795T1 (el) | 2015-10-07 |
| RS51724B (en) | 2011-10-31 |
| PL1890713T3 (pl) | 2011-09-30 |
| ES2362430T3 (es) | 2011-07-05 |
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