HK1226105B - Improved methods of nucleic acid sequencing - Google Patents

Improved methods of nucleic acid sequencing Download PDF

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Publication number
HK1226105B
HK1226105B HK16114306.2A HK16114306A HK1226105B HK 1226105 B HK1226105 B HK 1226105B HK 16114306 A HK16114306 A HK 16114306A HK 1226105 B HK1226105 B HK 1226105B
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HK
Hong Kong
Prior art keywords
dna
nucleic acid
rna
strand
sequence
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HK16114306.2A
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German (de)
English (en)
French (fr)
Chinese (zh)
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HK1226105A1 (en
Inventor
Niall Anthony Gormley
Louise Fraser
Paula KOKKO-GONZALES
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Illumina Cambridge Limited
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Publication of HK1226105A1 publication Critical patent/HK1226105A1/en
Publication of HK1226105B publication Critical patent/HK1226105B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
HK16114306.2A 2012-03-06 2015-07-09 Improved methods of nucleic acid sequencing HK1226105B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261607418P 2012-03-06 2012-03-06
US201261607418P 2012-03-06

Related Parent Applications (1)

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HK15106560.0A Addition HK1206072B (en) 2012-03-06 2013-03-06 Improved methods of nucleic acid sequencing

Related Child Applications (1)

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HK15106560.0A Division HK1206072B (en) 2012-03-06 2013-03-06 Improved methods of nucleic acid sequencing

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HK1226105A1 HK1226105A1 (en) 2017-09-22
HK1226105B true HK1226105B (en) 2017-09-22

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US (4) US9574226B2 (enExample)
EP (2) EP2823058B1 (enExample)
JP (2) JP6068517B2 (enExample)
CN (1) CN104160040B (enExample)
AU (1) AU2013229533B2 (enExample)
CA (1) CA2864276C (enExample)
DK (2) DK3037552T5 (enExample)
ES (1) ES2565809T3 (enExample)
HK (1) HK1226105B (enExample)
NO (1) NO2694769T3 (enExample)
TR (1) TR201807138T4 (enExample)
WO (1) WO2013131962A1 (enExample)

Families Citing this family (174)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102369298B (zh) 2009-01-30 2017-03-22 牛津纳米孔技术有限公司 跨膜测序中用于核酸构建体的衔接体
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN103827320B (zh) 2011-07-25 2017-04-26 牛津纳米孔技术有限公司 使用跨膜孔进行双链多核苷酸测序的发夹环方法
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN104769127A (zh) 2012-08-14 2015-07-08 10X基因组学有限公司 微胶囊组合物及方法
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3511423B2 (en) 2012-10-17 2024-05-29 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
CN105209634B (zh) 2013-03-08 2020-05-12 牛津纳米孔技术公司 酶停滞方法
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
AU2013382098B2 (en) 2013-03-13 2019-02-07 Illumina, Inc. Methods and compositions for nucleic acid sequencing
JP6618894B2 (ja) 2013-05-23 2019-12-11 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 個別的エピゲノミクスのための天然クロマチンへの転移
LT3013984T (lt) 2013-06-25 2023-06-12 Prognosys Biosciences, Inc. Biologinių taikinių erdvinių modelių mėginyje nustatymo būdai
US20160199832A1 (en) 2013-08-30 2016-07-14 Advanced Liquid Logic France Sas Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
CN114717291A (zh) * 2013-12-30 2022-07-08 阿特雷卡公司 使用核酸条形码分析与单细胞缔合的核酸
JP6525342B2 (ja) * 2014-01-14 2019-06-05 キアゲン ゲーエムベーハー タグ付けされたｄｎａ断片の生成
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
EP3137601B1 (en) 2014-04-29 2020-04-08 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
SG11201609055RA (en) * 2014-05-08 2016-11-29 Fluidigm Corp Integrated single cell sequencing
US20150353989A1 (en) * 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
EP3155125A1 (en) 2014-06-13 2017-04-19 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
GB201410646D0 (en) * 2014-06-14 2014-07-30 Illumina Cambridge Ltd Methods of increasing sequencing accuracy
CN118910216A (zh) 2014-06-26 2024-11-08 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US20150376700A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Analysis of nucleic acid sequences
US10017759B2 (en) * 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
DK3161152T3 (en) * 2014-06-30 2019-03-25 Illumina Inc Methods and compositions using one-sided transposition
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
SG11201701974XA (en) 2014-10-09 2017-04-27 Illumina Inc Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN106715691B (zh) * 2014-10-14 2020-06-02 深圳华大智造科技有限公司 一种转座酶打断核酸并加接头的方法和试剂
EP3207134B1 (en) * 2014-10-17 2019-07-03 Illumina Cambridge Limited Contiguity preserving transposition
US11873480B2 (en) * 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
CA2964472A1 (en) 2014-10-29 2016-05-06 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (en) 2014-12-18 2018-08-29 Agilome, Inc. Chemically-sensitive field effect transistor
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
CN107532202A (zh) 2015-02-24 2018-01-02 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3271073B1 (en) 2015-03-20 2019-06-12 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
EP3303621B1 (en) * 2015-05-28 2025-07-02 Illumina Cambridge Limited Surface-based tagmentation
US10857537B2 (en) 2015-07-06 2020-12-08 Illumina, Inc. Balanced AC modulation for driving droplet operations electrodes
EP3320111B1 (en) 2015-07-06 2021-05-05 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
EP3334839B1 (en) 2015-08-14 2021-03-03 Illumina, Inc. Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
EP3341494B1 (en) 2015-08-28 2024-08-07 Illumina, Inc. Nucleic acid sequence analysis from single cells
HK1253910A1 (zh) 2015-09-02 2019-07-05 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
SG11201803293SA (en) 2015-10-22 2018-05-30 Illumina Inc Filler fluid for fluidic devices
AU2016364722B2 (en) 2015-12-01 2020-10-22 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
CA3006792A1 (en) * 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
WO2017172798A1 (en) 2016-03-28 2017-10-05 Illumina, Inc. Multi-plane microarrays
CA3016221C (en) 2016-04-07 2021-09-28 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
EP4029950A1 (en) 2016-04-29 2022-07-20 Board of Regents, The University of Texas System Targeted measure of transcriptional activity related to hormone receptors
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2018027048A1 (en) * 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
CA3042393A1 (en) 2016-11-03 2018-05-11 Complete Genomics, Inc. Biosensors for biological or chemical analysis and methods of manufacturing the same
KR102586847B1 (ko) 2016-12-22 2023-10-10 일루미나, 인코포레이티드 시퀀싱 프라이머 및 비-시퀀싱 존재물을 포함하는 어레이
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
DK3452621T3 (da) 2017-02-21 2022-12-12 Illumina Inc Tagmentation ved brug af immobiliserede transposomer med linkere
CN110506336B (zh) 2017-03-20 2024-02-02 深圳华大智造科技股份有限公司 用于生物或化学分析的生物传感器及其制造方法
CN110462058A (zh) * 2017-03-24 2019-11-15 新加坡国立大学 用于多重检测分子的方法
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
WO2019060177A1 (en) 2017-09-19 2019-03-28 Complete Genomics, Inc. MANUFACTURE OF SEQUENCING FLOW CELLS AT THE WAFER
WO2019076768A1 (en) 2017-10-16 2019-04-25 Tervisetehnoloogiate Arenduskeskus As METHOD AND KIT FOR PREPARING DNA BANK
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
KR20210133298A (ko) * 2017-12-21 2021-11-05 일루미나, 인코포레이티드 하이드로겔 코팅을 가진 플로우 셀
US12377396B2 (en) 2018-02-05 2025-08-05 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
SG11202100765UA (en) 2018-08-15 2021-03-30 Illumina Inc Compositions and methods for improving library enrichment
CN120775968A (zh) * 2018-08-28 2025-10-14 10X基因组学股份有限公司 用于生物样品中转座酶介导的空间标记和分析基因组dna的方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113767176A (zh) 2018-12-10 2021-12-07 10X基因组学有限公司 成像系统硬件
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
CN113574181A (zh) * 2019-03-01 2021-10-29 武汉华大医学检验所有限公司 用于rna直接建库的核酸序列、基于rna样本直接构建测序文库的方法及应用
KR20220004947A (ko) 2019-04-29 2022-01-12 일루미나, 인코포레이티드 신속한 인큐베이션 및 핵산 풍부화에 의한 미생물 샘플의 확인 및 분석
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP3997224A1 (en) * 2019-07-12 2022-05-18 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
US11919000B2 (en) 2019-10-10 2024-03-05 1859, Inc. Methods and systems for microfluidic screening
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
SG11202106899SA (en) 2019-12-23 2021-09-29 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021236625A1 (en) 2020-05-19 2021-11-25 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4600376A3 (en) 2020-06-02 2025-10-22 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
AU2021288090A1 (en) 2020-06-10 2023-01-19 10X Genomics, Inc. Fluid delivery methods
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
KR20230041725A (ko) 2020-08-06 2023-03-24 일루미나, 인코포레이티드 비드-연결된 트랜스포좀을 사용한 rna 및 dna 시퀀싱 라이브러리의 제작
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
EP4301870B1 (en) 2021-03-18 2025-01-15 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20220411864A1 (en) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
WO2023034076A1 (en) 2021-08-31 2023-03-09 Illumina, Inc. Flow cell with enhanced well imaging resolution
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
WO2023056328A2 (en) * 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US20230193147A1 (en) 2021-12-21 2023-06-22 Illumina Cambridge Limited Wax-microsphere matrix compositions and methods of making and using the same
US20230348967A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5523221A (en) * 1993-06-16 1996-06-04 Stratagene Method for the directional cloning of DNA
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
AU747242B2 (en) 1997-01-08 2002-05-09 Proligo Llc Bioconjugation of macromolecules
ES2563643T3 (es) 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
WO1999002671A1 (en) 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
WO2003093501A2 (en) * 2002-04-30 2003-11-13 University Of Bremen Ssh based methods for identifying and isolating unique nucleic acid sequences
JP4638225B2 (ja) 2002-05-30 2011-02-23 ザ スクリプス リサーチ インスティテュート 銅を触媒とするアジドとアセチレンのライゲーション
ES2550513T3 (es) 2002-08-23 2015-11-10 Illumina Cambridge Limited Nucleótidos modificados para secuenciación de polinucleótidos
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP1594981B1 (en) 2003-01-29 2010-03-17 454 Corporation Double ended sequencing
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7259258B2 (en) 2003-12-17 2007-08-21 Illumina, Inc. Methods of attaching biological compounds to solid supports using triazine
WO2005065814A1 (en) 2004-01-07 2005-07-21 Solexa Limited Modified molecular arrays
US7302146B2 (en) 2004-09-17 2007-11-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
CA2648149A1 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20090226975A1 (en) 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
ES2403756T3 (es) * 2008-10-24 2013-05-21 Epicentre Technologies Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2272976A1 (en) 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
US9029103B2 (en) * 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
BR112013002299A2 (pt) 2010-08-27 2016-05-24 Genentech Inc método de captura e sequenciamento de uma molécula alvo de ácido nucleíco e método de determinação do estado de metilação de um fragmento de dna genônimo
US9238671B2 (en) * 2011-01-28 2016-01-19 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
EP2691541B1 (en) * 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences

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