HK1217736A1 - High throughput nucleic acid sequencing by expansion - Google Patents

High throughput nucleic acid sequencing by expansion Download PDF

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Publication number
HK1217736A1
HK1217736A1 HK16105793.0A HK16105793A HK1217736A1 HK 1217736 A1 HK1217736 A1 HK 1217736A1 HK 16105793 A HK16105793 A HK 16105793A HK 1217736 A1 HK1217736 A1 HK 1217736A1
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HK
Hong Kong
Prior art keywords
tether
subunit
xpandomer
template
probe
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HK16105793.0A
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English (en)
Chinese (zh)
Inventor
Stamatios Kokoris Mark
N. Mcruer Robert
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Stratos Genomics Inc.
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Application filed by Stratos Genomics Inc. filed Critical Stratos Genomics Inc.
Publication of HK1217736A1 publication Critical patent/HK1217736A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK16105793.0A 2007-06-19 2010-10-05 High throughput nucleic acid sequencing by expansion HK1217736A1 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US305P 1995-06-19
US94503107P 2007-06-19 2007-06-19
US945031P 2007-06-19
US98191607P 2007-10-23 2007-10-23
US981916P 2007-10-23
US30507P 2007-10-25 2007-10-25

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
HK10109489.7A Addition HK1143188B (en) 2007-06-19 2008-06-19 High throughput nucleic acid sequencing by expansion

Related Child Applications (1)

Application Number Title Priority Date Filing Date
HK10109489.7A Division HK1143188B (en) 2007-06-19 2008-06-19 High throughput nucleic acid sequencing by expansion

Publications (1)

Publication Number Publication Date
HK1217736A1 true HK1217736A1 (en) 2017-01-20

Family

ID=39986452

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16105793.0A HK1217736A1 (en) 2007-06-19 2010-10-05 High throughput nucleic acid sequencing by expansion

Country Status (12)

Country Link
US (8) US7939259B2 (https=)
EP (2) EP2952587B1 (https=)
JP (1) JP5745842B2 (https=)
KR (1) KR101685208B1 (https=)
CN (1) CN102083998B (https=)
AU (1) AU2008265691B2 (https=)
CA (1) CA2691364C (https=)
DK (1) DK2171088T3 (https=)
ES (2) ES2559313T3 (https=)
HK (1) HK1217736A1 (https=)
IL (2) IL202821A (https=)
WO (1) WO2008157696A2 (https=)

Families Citing this family (217)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005118773A2 (en) 2004-05-28 2005-12-15 Wafergen, Inc. Apparatus and methods for multiplex analyses
WO2007041621A2 (en) * 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
AU2006336311B2 (en) * 2006-01-19 2013-01-24 Allexcel, Inc. Solubilization and targeted delivery of drugs with self-assembling amphiphilic polymers
WO2007146158A1 (en) * 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
DK2171088T3 (en) * 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
EP2201136B1 (en) 2007-10-01 2017-12-06 Nabsys 2.0 LLC Nanopore sequencing by hybridization of probes to form ternary complexes and variable range alignment
WO2009055617A1 (en) * 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
CN102186989B (zh) * 2008-09-03 2021-06-29 纳伯塞斯2.0有限责任公司 用于流体通道中生物分子和其它分析物的电压感测的纵向移位纳米级电极的使用
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
WO2010028288A2 (en) 2008-09-05 2010-03-11 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
EP2391726B1 (en) * 2009-01-29 2015-09-23 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion and related methods
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
WO2010111605A2 (en) * 2009-03-27 2010-09-30 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
WO2010114842A1 (en) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
EP2483680A4 (en) 2009-09-30 2014-01-01 Quantapore Inc ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
WO2012003330A2 (en) 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
EP2619329B1 (en) 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
US8865404B2 (en) * 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
US8859201B2 (en) 2010-11-16 2014-10-14 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
US8805095B2 (en) 2010-12-03 2014-08-12 International Business Machines Corporation Analysing character strings
GB2500360B (en) 2010-12-22 2019-10-23 Genia Tech Inc Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN103502463B (zh) 2010-12-27 2016-03-16 艾比斯生物科学公司 核酸样品的制备方法及组合物
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2012109574A2 (en) * 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
US9670538B2 (en) 2011-08-05 2017-06-06 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
JP6457811B2 (ja) 2011-09-23 2019-01-23 オックスフォード ナノポール テクノロジーズ リミテッド ポリマー単位を含むポリマーの解析
EP3604555B1 (en) * 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US9803188B2 (en) 2011-12-22 2017-10-31 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
EP4249605B1 (en) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Methods for analyte detection
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013101935A1 (en) 2011-12-27 2013-07-04 Ibis Biosciences, Inc. Bioagent detection oligonucleotides
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
US9803231B2 (en) 2011-12-29 2017-10-31 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
US9822417B2 (en) 2012-01-09 2017-11-21 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
EP2802666B1 (en) 2012-01-13 2018-09-19 Data2Bio Genotyping by next-generation sequencing
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US10202642B2 (en) 2012-05-02 2019-02-12 Ibis Biosciences, Inc. DNA sequencing
EP2844767A4 (en) 2012-05-02 2015-11-18 Ibis Biosciences Inc SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
WO2013188841A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
EP2875458A2 (en) 2012-07-19 2015-05-27 President and Fellows of Harvard College Methods of storing information using nucleic acids
EP2909343B1 (en) 2012-10-16 2018-10-10 Abbott Molecular Inc. Methods to sequence a nucleic acid
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
WO2014074922A1 (en) 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
GB201222928D0 (en) * 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding
US11505587B2 (en) * 2013-01-18 2022-11-22 Nanyang Technological University Method of preparing a keratin-based biomaterial and keratin-based biomaterial formed thereof
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
EP2971184B1 (en) 2013-03-12 2019-04-17 President and Fellows of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US20140287946A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
EP2971171A4 (en) 2013-03-14 2016-11-02 Abbott Molecular Inc SYSTEMS AND METHODS FOR MULTIPLEX AMPLIFICATION SPECIFIC TO METHYLATION
WO2014150910A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
WO2014151511A2 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
CA2910019A1 (en) * 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
SG10201913068PA (en) 2013-06-04 2020-02-27 Harvard College Rna-guided transcriptional regulation
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
ES2764096T3 (es) 2013-08-19 2020-06-02 Abbott Molecular Inc Bibliotecas de secuenciación de próxima generación
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US10174381B2 (en) 2013-10-18 2019-01-08 The Regents Of The University Of Michigan Systems and methods for determining a treatment course of action
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
JP6461943B2 (ja) 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
US10768181B2 (en) 2013-12-17 2020-09-08 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
EP3594363A1 (en) * 2014-04-23 2020-01-15 Children's Medical Center Corporation High-throughput structure determination using nucleic acid calipers
US20150315637A1 (en) * 2014-04-30 2015-11-05 International Business Machines Corporation Bow tie dna compositions and methods
US10934581B2 (en) 2014-04-30 2021-03-02 International Business Machines Corporation Bow tie DNA compositions and methods
EP3143158A1 (en) 2014-05-14 2017-03-22 Stratos Genomics, Inc. Translocation control for sensing by a nanopore
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
WO2015200541A1 (en) 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
ES2753573T3 (es) 2014-06-27 2020-04-13 Abbott Lab Composiciones y métodos para detectar pegivirus humano 2 (HPGV-2)
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
AP2017009731A0 (en) 2014-07-24 2017-02-28 Abbott Molecular Inc Compositions and methods for the detection and analysis of mycobacterium tuberculosis
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
KR20170064540A (ko) * 2014-09-26 2017-06-09 투 포어 가이즈, 인코포레이티드 합성 프로브의 나노포어 감지에 의한 표적 서열 검출
US20170247756A1 (en) * 2014-10-03 2017-08-31 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
ES2789000T3 (es) 2014-10-10 2020-10-23 Quantapore Inc Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente
EP4397972A3 (en) 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
CN107002126B (zh) 2014-10-24 2021-05-25 昆塔波尔公司 使用纳米结构阵列的聚合物的高效光学分析
AU2015349730C1 (en) 2014-11-20 2020-04-30 F. Hoffmann-La Roche Ag Nulceoside phosphoroamidate esters and derivatives thereof, use and synthesis thereof
CN107075564A (zh) * 2014-12-10 2017-08-18 深圳华大基因研究院 确定肿瘤核酸浓度的方法和装置
CN107077538B (zh) * 2014-12-10 2020-08-07 深圳华大生命科学研究院 测序数据处理装置和方法
WO2016090583A1 (zh) * 2014-12-10 2016-06-16 深圳华大基因研究院 测序数据处理装置和方法
WO2016112073A1 (en) * 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
JP6920275B2 (ja) * 2015-07-13 2021-08-18 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸を用いた回収可能な情報記憶のための方法
ES2902125T3 (es) 2015-07-14 2022-03-25 Abbott Molecular Inc Purificación de ácidos nucleicos utilizando óxidos de cobre-titanio u óxidos de magnesio-titanio
EP3322483A4 (en) 2015-07-14 2019-01-02 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
CN108603235A (zh) 2015-07-30 2018-09-28 马萨诸塞眼科耳科诊所 祖先病毒序列及其用途
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
JP7064439B2 (ja) * 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
EP3377648B1 (en) 2015-11-16 2021-01-06 Stratos Genomics, Inc. Dp04 polymerase variants
CN108431223A (zh) 2016-01-08 2018-08-21 生物辐射实验室股份有限公司 每液滴分辨率下的多个珠
AU2017218431B2 (en) 2016-02-08 2022-08-04 RGENE, Inc. Multiple ligase compositions, systems, and methods
EP3414347A4 (en) * 2016-02-11 2019-10-09 Qiagen Sciences, LLC ADDITIVE FOR IMPROVING SEQUENCING THROUGH SYNTHETIC PERFORMANCE
EP3430154B1 (en) 2016-03-14 2020-11-11 Rgene, Inc. Hyper-thermostable lysine-mutant ssdna/rna ligases
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
JP2019522983A (ja) 2016-07-05 2019-08-22 クアンタポール， インコーポレイテッド 光学ベースのナノポア配列決定
WO2018013509A1 (en) 2016-07-11 2018-01-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
US10781175B2 (en) 2016-07-15 2020-09-22 Am Chemicals Llc Solid supports and phosphoramidite building blocks for oligonucleotide conjugates
JP7075394B2 (ja) 2016-07-21 2022-05-25 タカラ バイオ ユーエスエー， インコーポレイテッド マルチウェルデバイスを用いたマルチｚ撮像及び分注
US11543417B2 (en) 2016-08-29 2023-01-03 Oslo Universitetssykehus Hf ChIP-seq assays
CN118389650A (zh) 2016-08-31 2024-07-26 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
CN118853848A (zh) 2016-08-31 2024-10-29 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
US12116570B2 (en) 2016-09-29 2024-10-15 Roche Sequencing Solutions, Inc. Directed evolution of enzymes by plasmid tagging in droplets
EP3526349B1 (en) 2016-10-13 2021-03-17 F. Hoffmann-La Roche AG Molecular detection and counting using nanopores
CN110139932B (zh) 2016-12-19 2024-05-17 生物辐射实验室股份有限公司 液滴加标的相邻性保留的标签化dna
US11708566B2 (en) 2017-05-04 2023-07-25 Stratos Genomics, Inc. DP04 polymerase variants
EP3634992B1 (en) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
US11390856B2 (en) 2017-08-07 2022-07-19 Dna Script Variants of family a DNA polymerase and uses thereof
WO2019089959A1 (en) 2017-11-02 2019-05-09 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
US11131646B2 (en) 2017-11-03 2021-09-28 Robert Bosch Gmbh Electrochemical sequencing of DNA using an edge electrode
CA3084812A1 (en) 2017-12-11 2019-06-20 Stratos Genomics, Inc. Dpo4 polymerase variants with improved accuracy
EP3728635A1 (en) * 2017-12-21 2020-10-28 F. Hoffmann-La Roche AG Compositions and methods for unidirectional nucleic acid sequencing
WO2019135975A1 (en) * 2018-01-05 2019-07-11 Stratos Genomics Inc. Enhancement of nucleic acid polymerization by aromatic compounds
CN111712584B (zh) 2018-01-05 2025-02-07 F·霍夫曼-罗氏股份公司 由小沟结合部分增强核酸聚合
US10435676B2 (en) 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
JP6559814B2 (ja) * 2018-01-26 2019-08-14 株式会社日立ハイテクノロジーズ 生体分子測定方法
WO2019152395A1 (en) 2018-01-31 2019-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US12037707B2 (en) 2018-04-05 2024-07-16 Massachusetts Eye And Ear Infirmary Methods of making and using combinatorial barcoded nucleic acid libraries having defined variation
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
EP3826762A1 (en) 2018-07-23 2021-06-02 DNA Script Massively parallel enzymatic synthesis of nucleic acid strands
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
EP4249651B1 (en) 2018-08-20 2025-01-29 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US12139764B2 (en) 2018-11-14 2024-11-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating breast cancer
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
WO2020165137A1 (en) 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CN113631764A (zh) * 2019-02-21 2021-11-09 斯特拉托斯基因公司 用于固态合成在单分子测序中使用的可扩展聚合物的方法、组合物和装置
WO2020236526A1 (en) 2019-05-23 2020-11-26 Stratos Genomics, Inc. Translocation control elements, reporter codes, and further means for translocation control for use in nanopore sequencing
CN114040975A (zh) 2019-06-07 2022-02-11 生物辐射实验室股份有限公司 每液滴多个珠的解决方案
WO2020254672A1 (en) 2019-06-19 2020-12-24 Therycell Gmbh Spatial characterisation of target structures in a sample
US11755922B2 (en) * 2019-10-04 2023-09-12 The Board Of Trustees Of The University Of Illinois On-chip nanoscale storage system using chimeric DNA
CN114630891B (zh) 2019-10-30 2025-06-10 豪夫迈·罗氏有限公司 用于生物纳米孔的组装的方法和组合物
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のｄｎａ配列を同定する方法
EP4097251A1 (en) 2020-01-29 2022-12-07 10X Genomics, Inc. Compositions and methods for analyte detection
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
WO2021249985A1 (en) 2020-06-10 2021-12-16 F. Hoffmann-La Roche Ag Faradaic systems and methods for self-limiting protein pore insertion in a membrane
WO2022020359A1 (en) 2020-07-24 2022-01-27 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN112201305B (zh) * 2020-10-12 2021-12-07 冬青南京生物科技有限公司 一种单细胞核中基因数目测定设备
GB202103605D0 (en) 2021-03-16 2021-04-28 Oxford Nanopore Tech Ltd Alignment of target and reference sequences of polymer units
US20240377380A1 (en) 2021-03-31 2024-11-14 Illumina, Inc. Nanopore sensor devices
US20230014008A1 (en) * 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for improving spatial performance
WO2023059599A1 (en) 2021-10-04 2023-04-13 F. Hoffmann-La Roche Ag Online base call compression
WO2023081031A1 (en) 2021-11-08 2023-05-11 Illumina, Inc. Identifying nucleotides using changes in impedance between electrodes
JP2025510288A (ja) 2022-03-28 2025-04-14 エフ． ホフマン－ラ ロシュ アーゲー 異性体的に純粋なポリオール系ホスホロアミダイトの合成
JP2025513682A (ja) 2022-03-31 2025-04-30 イルミナ インコーポレイテッド ジブロック又はトリブロックコポリマーを使用するバリアを含むナノポアデバイス、及びその作製方法
WO2023187001A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Devices including osmotically balanced barriers, and methods of making and using the same
US20230381718A1 (en) 2022-03-31 2023-11-30 Illumina Cambridge Limited Barriers including molecules covalently bonded to amphiphilic molecules, and methods of making the same
EP4499281A1 (en) 2022-03-31 2025-02-05 Illumina, Inc. Barriers including cross-linked amphiphilic molecules, and methods of making the same
WO2023187081A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Methods for inserting nanopores into polymeric membranes using chaotropic solvents
US20230312857A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Nanopore devices including barriers using polymers with end groups, and methods of making the same
US20250205655A1 (en) 2022-03-31 2025-06-26 Illumina, Inc. Amphiphilic polymers to be used in barriers and preparation thereof, barriers with nanopores and preparation thereof
CN119137286A (zh) 2022-05-02 2024-12-13 豪夫迈·罗氏有限公司 在纳米孔测序期间减少普鲁士蓝形成的组合物和方法
EP4599083A1 (en) 2022-10-03 2025-08-13 F. Hoffmann-La Roche AG Single molecule multi-molecular trace methods and systems
WO2024083982A1 (en) 2022-10-21 2024-04-25 F. Hoffmann-La Roche Ag Detection of modified nucleobases in nucleic acid samples
AU2024207414A1 (en) 2023-01-13 2025-06-12 F. Hoffmann-La Roche Ag Detection of modified nucleobases in dna samples
AU2024246785A1 (en) 2023-03-30 2025-09-11 F. Hoffmann-La Roche Ag Modulation of target molecule-lipid bilayer interactions
EP4705375A1 (en) 2023-05-04 2026-03-11 F. Hoffmann-La Roche AG Modified triblock copolymer compounds and methods of use thereof
EP4705012A1 (en) 2023-05-04 2026-03-11 F. Hoffmann-La Roche AG Hybrid triblock copolymer membrane compositions and methods for nanopore sequencing
WO2024242847A1 (en) 2023-05-25 2024-11-28 Illumina, Inc. Methods of making barriers including nanopores and cross¬ linked amphiphilic molecules, and barriers formed using same
WO2025082960A1 (en) 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties
US20250137033A1 (en) * 2023-11-01 2025-05-01 Robert Bosch Gmbh Dna unfolding using a free-end tag flow modifier
WO2025106652A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Fluidic devices, nanopore instruments, and methods
WO2025137293A2 (en) 2023-12-22 2025-06-26 Roche Sequencing Solutions, Inc. Enhancement of nucleic acid polymerization by aromatic compounds
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025132780A2 (en) * 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025149478A1 (en) 2024-01-12 2025-07-17 F. Hoffmann-La Roche Ag Compositions of modified nucleoside triphosphates
WO2025149479A1 (en) 2024-01-12 2025-07-17 Roche Diagnostics Gmbh Synthesis of modified nucleoside triphosphates
WO2025174659A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Net-zero current scheme for nanopore system
WO2025174660A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Recharging for nanopore system
EP4603180A1 (en) 2024-02-15 2025-08-20 Roche Diagnostics International AG Techniques for synthesizing a macromolecule from a sample
WO2025212586A1 (en) * 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads
WO2025240460A2 (en) 2024-05-14 2025-11-20 Roche Molecular Systems, Inc. Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent
WO2026033006A1 (en) 2024-08-07 2026-02-12 Roche Sequencing Solutions, Inc. Nickase-mediated linear amplification of library constructs for duplex sequencing
WO2026033007A1 (en) 2024-08-07 2026-02-12 F. Hoffmann-La Roche Ag Automated solid-state amplification of library constructs for duplex sequencing
WO2026050541A1 (en) 2024-08-28 2026-03-05 Roche Sequencing Solutions, Inc. Methods and systems for allele-specific copy number calling
WO2026046950A1 (en) 2024-08-28 2026-03-05 F. Hoffmann-La Roche Ag Methods and compositions for size-selective elution of xpandomer macromolecules for nanopore sequencing
WO2026050290A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Lipid composition and methods of use in a nanopore sequencing assay
WO2026050289A1 (en) 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Pre-and post-sequencing cleaning protocols for nanopore sequencing
WO2026050292A1 (en) * 2024-08-29 2026-03-05 Roche Sequencing Solutions, Inc. Optimized reagent introduction during a nanopore sequencing assay
WO2026050121A1 (en) 2024-08-30 2026-03-05 Roche Sequencing Solutions, Inc. Compositions for replicating a nucleic acid template
WO2026050150A1 (en) 2024-08-30 2026-03-05 Roche Sequencing Solutions, Inc. Dynamic control of spatial and temporal variability in nanopore sequencing
WO2026050702A1 (en) 2024-08-31 2026-03-05 Roche Sequencing Solutions, Inc. Systems and methods for tuning sequencing parameters
WO2026060095A1 (en) 2024-09-16 2026-03-19 Roche Sequencing Solutions, Inc. Single cell rna sequencing methods and systems
CN118904278A (zh) * 2024-10-08 2024-11-08 祥符实验室 一种核酸固相合成提取磁珠及其制备方法和应用

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
JPH0874B2 (ja) 1990-07-27 1996-01-10 アイシス・ファーマシューティカルス・インコーポレーテッド 遺伝子発現を検出および変調するヌクレアーゼ耐性、ピリミジン修飾オリゴヌクレオチド
US5378825A (en) * 1990-07-27 1995-01-03 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
AU3222793A (en) 1991-11-26 1993-06-28 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
FR2702874B1 (fr) 1993-03-18 1995-06-09 Centre Nat Rech Scient Cellule memoire insensible aux rayonnements.
DK0691980T3 (da) 1993-03-30 1997-12-29 Sanofi Sa 7-deazapurinmodifiderende oligonukleotider
EP0695306A1 (en) 1993-04-19 1996-02-07 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
FR2705099B1 (fr) * 1993-05-12 1995-08-04 Centre Nat Rech Scient Oligonucléotides phosphorothioates triesters et procédé de préparation.
DE4438918A1 (de) * 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US6242589B1 (en) 1998-07-14 2001-06-05 Isis Pharmaceuticals, Inc. Phosphorothioate oligonucleotides having modified internucleoside linkages
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1196554B1 (en) * 1999-03-18 2009-02-25 Complete Genomics AS Methods of cloning and producing fragment chains with readable information content
EP2383776B1 (en) 1999-06-22 2015-02-25 President and Fellows of Harvard College Solid state nanopore device for evaluating biopolymers
EP1072679A3 (en) * 1999-07-20 2002-07-31 Agilent Technologies, Inc. (a Delaware corporation) Method of producing nucleic acid molecules with reduced secondary structure
US7060440B1 (en) 1999-09-01 2006-06-13 Yeda Research And Development Co. Ltd. Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US6951720B2 (en) 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
GB0308852D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
EP2230316A1 (en) * 2005-02-01 2010-09-22 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
DK2171088T3 (en) * 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
CN201189345Y (zh) * 2008-05-23 2009-02-04 北京航天长峰股份有限公司 一种氧浓度调节装置

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