HK1205534A1 - Method of dna detection and quantification by single-molecule hybridization and manipulation dna - Google Patents

Method of dna detection and quantification by single-molecule hybridization and manipulation dna

Info

Publication number
HK1205534A1
HK1205534A1 HK15106060.5A HK15106060A HK1205534A1 HK 1205534 A1 HK1205534 A1 HK 1205534A1 HK 15106060 A HK15106060 A HK 15106060A HK 1205534 A1 HK1205534 A1 HK 1205534A1
Authority
HK
Hong Kong
Prior art keywords
dna
quantification
manipulation
molecule hybridization
detection
Prior art date
Application number
HK15106060.5A
Other languages
English (en)
Chinese (zh)
Inventor
‧本西蒙
‧阿萊曼德
‧丁
‧克羅凱特
Original Assignee
Centre Nat Rech Scient
Ecole Normale Superieure
Univ Pierre Et Marie Curie Paris 6
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Ecole Normale Superieure, Univ Pierre Et Marie Curie Paris 6 filed Critical Centre Nat Rech Scient
Publication of HK1205534A1 publication Critical patent/HK1205534A1/xx

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK15106060.5A 2011-12-22 2015-06-25 Method of dna detection and quantification by single-molecule hybridization and manipulation dna HK1205534A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11306743 2011-12-22
PCT/EP2012/076644 WO2013093005A1 (en) 2011-12-22 2012-12-21 Method of dna detection and quantification by single-molecule hybridization and manipulation

Publications (1)

Publication Number Publication Date
HK1205534A1 true HK1205534A1 (en) 2015-12-18

Family

ID=47520089

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15106060.5A HK1205534A1 (en) 2011-12-22 2015-06-25 Method of dna detection and quantification by single-molecule hybridization and manipulation dna

Country Status (11)

Country Link
US (1) US9738924B2 (ja)
EP (1) EP2794915B1 (ja)
JP (1) JP6169603B2 (ja)
KR (1) KR101917272B1 (ja)
CN (1) CN104254617B (ja)
AU (1) AU2012356826B2 (ja)
BR (1) BR112014015547A2 (ja)
CA (1) CA2859913C (ja)
HK (1) HK1205534A1 (ja)
IL (1) IL233248B (ja)
WO (1) WO2013093005A1 (ja)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2390350A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by polymerisation
EP2390351A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation
CN107771222B (zh) 2015-05-07 2019-06-04 巴黎科学与文学联大-拉丁区 使用力诱导的链侵入原位形成发夹
EP3090803B1 (en) 2015-05-07 2019-08-07 Paris Sciences et Lettres - Quartier Latin Improved device for the analysis of nucleic acid molecules
US20160376584A1 (en) * 2015-06-29 2016-12-29 Genesis DNA Inc. Method and apparatus for dual solid phase nucleic acid synthesis
CN106650310B (zh) * 2017-01-09 2019-01-29 上海集爱遗传与不育诊疗中心 一种鉴别染色体平衡易位携带胚胎和正常胚胎的方法
EP3950957A1 (en) 2017-08-08 2022-02-09 Depixus In vitro isolation and enrichment of nucleic acids using site-specific nucleases
CA3120176A1 (en) 2018-11-16 2020-05-22 Depixus Optimization of in vitro isolation of nucleic acids using site-specific nucleases
IL283866B2 (en) 2018-12-12 2023-02-01 Depixus Nucleic acid enrichment method using site-specific nuclei, which appear by capture
US11555871B2 (en) 2019-12-31 2023-01-17 Industrial Technology Research Institute Method of detecting biological sample
EP4160199A1 (en) 2021-10-04 2023-04-05 Depixus Apparatus for biomolecule analysis with a well and a cavity below the well
CN117385002A (zh) * 2023-09-14 2024-01-12 中山大学 一种基于恒力模式下单分子力谱测量的核酸检测方法

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
ATE48140T1 (de) 1981-04-17 1989-12-15 Univ Yale Modifizierte nukleotide und verfahren zu ihrer herstellung und anwendung.
IL73577A (en) 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
CA1223222A (en) 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
EP0530357B1 (en) 1991-03-21 1995-11-15 Johnson & Johnson Clinical Diagnostics, Inc. Element and method for nucleic acid amplification and detection using adhered probes
DE69330750T2 (de) 1992-03-04 2002-07-04 Univ California Vergleichende genomhybridisierung
US5457027A (en) 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
AT401270B (de) 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US7244391B2 (en) 1997-03-28 2007-07-17 Centre National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
US7052650B2 (en) 1997-03-28 2006-05-30 Center National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
WO2002031463A2 (en) 2000-08-31 2002-04-18 Motorola, Inc. High density column and row addressable electrode arrays
EP2180064A3 (en) 2000-10-18 2010-08-11 Pharmasset, Inc. Multiplex quantification of nucleic acids in diseased cells
JP2008507952A (ja) * 2004-03-05 2008-03-21 リサーチ ファウンデーション オブ ステイト ユニヴァーシティー オブ ニューヨーク 情報依存性分子モーターとしてのｒｎａポリメラーゼの使用
GB0426188D0 (en) 2004-11-30 2004-12-29 Leuven K U Res & Dev DNA repeats
JP2011036150A (ja) * 2009-08-07 2011-02-24 Olympus Corp 標的核酸分子の定量方法及び標的核酸分子定量キット
EP2390351A1 (en) * 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation

Also Published As

Publication number Publication date
IL233248B (en) 2018-08-30
KR101917272B1 (ko) 2018-11-09
CA2859913C (en) 2021-02-23
EP2794915B1 (en) 2017-02-08
JP2015502754A (ja) 2015-01-29
KR20140124752A (ko) 2014-10-27
CA2859913A1 (en) 2013-06-27
AU2012356826B2 (en) 2017-08-17
CN104254617B (zh) 2017-04-26
CN104254617A (zh) 2014-12-31
US20150031028A1 (en) 2015-01-29
EP2794915A1 (en) 2014-10-29
AU2012356826A1 (en) 2014-07-24
WO2013093005A1 (en) 2013-06-27
BR112014015547A2 (pt) 2017-06-13
JP6169603B2 (ja) 2017-07-26
US9738924B2 (en) 2017-08-22
IL233248A0 (en) 2014-08-31

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