HK1205534A1 - 通過單分子雜交和操作進行的 檢測和定量的方法 - Google Patents

通過單分子雜交和操作進行的 檢測和定量的方法

Info

Publication number
HK1205534A1
HK1205534A1 HK15106060.5A HK15106060A HK1205534A1 HK 1205534 A1 HK1205534 A1 HK 1205534A1 HK 15106060 A HK15106060 A HK 15106060A HK 1205534 A1 HK1205534 A1 HK 1205534A1
Authority
HK
Hong Kong
Prior art keywords
dna
quantification
manipulation
molecule hybridization
detection
Prior art date
Application number
HK15106060.5A
Other languages
English (en)
Inventor
‧本西蒙
‧阿萊曼德
‧丁
‧克羅凱特
Original Assignee
Centre Nat Rech Scient
Ecole Normale Superieure
Univ Pierre Et Marie Curie Paris 6
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Ecole Normale Superieure, Univ Pierre Et Marie Curie Paris 6 filed Critical Centre Nat Rech Scient
Publication of HK1205534A1 publication Critical patent/HK1205534A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
HK15106060.5A 2011-12-22 2015-06-25 通過單分子雜交和操作進行的 檢測和定量的方法 HK1205534A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11306743 2011-12-22
PCT/EP2012/076644 WO2013093005A1 (en) 2011-12-22 2012-12-21 Method of dna detection and quantification by single-molecule hybridization and manipulation

Publications (1)

Publication Number Publication Date
HK1205534A1 true HK1205534A1 (zh) 2015-12-18

Family

ID=47520089

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15106060.5A HK1205534A1 (zh) 2011-12-22 2015-06-25 通過單分子雜交和操作進行的 檢測和定量的方法

Country Status (11)

Country Link
US (1) US9738924B2 (zh)
EP (1) EP2794915B1 (zh)
JP (1) JP6169603B2 (zh)
KR (1) KR101917272B1 (zh)
CN (1) CN104254617B (zh)
AU (1) AU2012356826B2 (zh)
BR (1) BR112014015547A2 (zh)
CA (1) CA2859913C (zh)
HK (1) HK1205534A1 (zh)
IL (1) IL233248B (zh)
WO (1) WO2013093005A1 (zh)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2390351A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation
EP2390350A1 (en) 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by polymerisation
EP3292217B1 (en) 2015-05-07 2019-07-10 Paris Sciences et Lettres - Quartier Latin Formation of hairpins in situ using force-induced strand invasion
EP3090803B1 (en) 2015-05-07 2019-08-07 Paris Sciences et Lettres - Quartier Latin Improved device for the analysis of nucleic acid molecules
US20160376584A1 (en) * 2015-06-29 2016-12-29 Genesis DNA Inc. Method and apparatus for dual solid phase nucleic acid synthesis
CN106650310B (zh) * 2017-01-09 2019-01-29 上海集爱遗传与不育诊疗中心 一种鉴别染色体平衡易位携带胚胎和正常胚胎的方法
EP3950957A1 (en) 2017-08-08 2022-02-09 Depixus In vitro isolation and enrichment of nucleic acids using site-specific nucleases
EP3844302A1 (en) 2018-11-16 2021-07-07 Depixus Optimization of in vitro isolation of nucleic acids using site-specific nucleases
DK3837379T3 (da) 2018-12-12 2022-06-13 Depixus Fremgangsmåde til nukleinsyreberigelse under anvendelse af stedspecifikke nukleaser efterfulgt af opfangning
US11555871B2 (en) 2019-12-31 2023-01-17 Industrial Technology Research Institute Method of detecting biological sample
EP4160199A1 (en) 2021-10-04 2023-04-05 Depixus Apparatus for biomolecule analysis with a well and a cavity below the well
CN117385002A (zh) * 2023-09-14 2024-01-12 中山大学 一种基于恒力模式下单分子力谱测量的核酸检测方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE48140T1 (de) 1981-04-17 1989-12-15 Univ Yale Modifizierte nukleotide und verfahren zu ihrer herstellung und anwendung.
IL73577A (en) 1983-12-12 1989-10-31 Miles Lab Method and reagent system for detecting dna or rna sequences in a test medium containing single stranded dna or rna using antibodies to intercalation complexes with double stranded nucleic acid
CA1223222A (en) 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
JPH0830704B2 (ja) 1991-03-21 1996-03-27 イーストマン コダック カンパニー 核酸増幅のための要素および方法ならびに付着プローブを使用する検出方法
ATE205542T1 (de) 1992-03-04 2001-09-15 Univ California Vergleichende genomhybridisierung
US5457027A (en) 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
AT401270B (de) 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5871697A (en) 1995-10-24 1999-02-16 Curagen Corporation Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US7244391B2 (en) 1997-03-28 2007-07-17 Centre National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
US7052650B2 (en) 1997-03-28 2006-05-30 Center National De La Recherche Scientifique (Cnrs) Apparatus and method for the manipulation and testing of molecules, and in particular of DNA
WO2002031463A2 (en) 2000-08-31 2002-04-18 Motorola, Inc. High density column and row addressable electrode arrays
NZ575481A (en) 2000-10-18 2010-10-29 Pharmasset Inc Simultaneous quantification of nucleic acids in diseased cells
WO2005098038A2 (en) * 2004-03-05 2005-10-20 Research Foundation Of State University Of New York Use of rna polymerase as an information-dependent molecular motor
GB0426188D0 (en) 2004-11-30 2004-12-29 Leuven K U Res & Dev DNA repeats
JP2011036150A (ja) * 2009-08-07 2011-02-24 Olympus Corp 標的核酸分子の定量方法及び標的核酸分子定量キット
EP2390351A1 (en) * 2010-05-27 2011-11-30 Centre National de la Recherche Scientifique (CNRS) Method of DNA sequencing by hybridisation

Also Published As

Publication number Publication date
EP2794915A1 (en) 2014-10-29
IL233248A0 (en) 2014-08-31
US20150031028A1 (en) 2015-01-29
CA2859913A1 (en) 2013-06-27
US9738924B2 (en) 2017-08-22
BR112014015547A2 (pt) 2017-06-13
CN104254617A (zh) 2014-12-31
AU2012356826A1 (en) 2014-07-24
IL233248B (en) 2018-08-30
CN104254617B (zh) 2017-04-26
CA2859913C (en) 2021-02-23
AU2012356826B2 (en) 2017-08-17
EP2794915B1 (en) 2017-02-08
KR101917272B1 (ko) 2018-11-09
KR20140124752A (ko) 2014-10-27
JP2015502754A (ja) 2015-01-29
WO2013093005A1 (en) 2013-06-27
JP6169603B2 (ja) 2017-07-26

Similar Documents

Publication Publication Date Title
HK1205534A1 (zh) 通過單分子雜交和操作進行的 檢測和定量的方法
HK1255232A1 (zh) 用於純化核酸的方法和試劑盒
HK1198547A1 (zh) 用於檢測核苷酸修飾的方法
HK1211260A1 (zh) 用於核酸測序的方法和試劑盒
GB2500120B (en) Method of producing fluid identification system including DNA
HK1201951A1 (zh) 序列的數據分析
EP2673380A4 (en) ANALYSIS OF NUCLEIC ACIDS
EP2798090A4 (en) NUCLEIC ACID ANALYSIS BY EMULSION PCR
EP2684031A4 (en) SYSTEM AND METHOD FOR FLUORESCENT AND ABSORPTION FORCE ANALYSIS
HK1199069A1 (zh) 基因的 分型方法和試劑盒
HK1183911A1 (zh) 通過雜交的 測序方法
EP2330220A4 (en) METHOD FOR QUANTIFYING OR DETERMINING DNA
IL236508A0 (en) Method and device for rapid identification of amplified nucleotide sequences
HK1183912A1 (zh) 通過聚合的 測序方法
EP2723906A4 (en) METHOD FOR REDUCING GENOMIC COMPLEXITY AND DETECTION OF POLYMORPHISM
EP2327799A4 (en) METHOD FOR DNA QUANTIFICATION OR DETECTION
EP2837695A4 (en) NUCLEIC ACID QUANTIFICATION METHOD, DETECTION PROBE, DETECTION CONDENSATE AND NUCLEIC ACID DETERGENT PROCEDURE
SI2854981T1 (sl) Metode frakcioniranja in detekcije nukleinske kisline
EP2836607A4 (en) RECOGNITION OF RESTRICTIONS OF LIGHT IMMUNOGLOBULIN CHAINS BY IN SITU RNA HYBRIDIZATION
EP2674492A4 (en) METHOD FOR DETECTING NUCLEIC ACIDS
EP2740804A4 (en) PROCESS FOR NUCLEIC ACID ANALYSIS
EP2865764A4 (en) METHOD OF DETECTING NUCLEIC ACID AND DETECTION KIT THEREFOR
EP2735618A4 (en) NUCLEIC ACID ANALYSIS METHOD AND ANALYSIS DEVICE
PT2748320T (pt) Composições, métodos e kits para hibridação de ácidos nucleicos
EP2692869A4 (en) DETECTION PROCESS FOR NUCLEIC ACID AND KIT AND ITS USE

Legal Events

Date Code Title Description
CHRG Changes in the register

Free format text: CORRECTION OF THE INTERNATIONAL APPLICATION FILING DATE