HK1201554A1 - Isoprene synthase variants with improved solubility for production of isoprene - Google Patents

Isoprene synthase variants with improved solubility for production of isoprene Download PDF

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Publication number
HK1201554A1
HK1201554A1 HK15101956.3A HK15101956A HK1201554A1 HK 1201554 A1 HK1201554 A1 HK 1201554A1 HK 15101956 A HK15101956 A HK 15101956A HK 1201554 A1 HK1201554 A1 HK 1201554A1
Authority
HK
Hong Kong
Prior art keywords
isoprene
production
improved solubility
synthase variants
isoprene synthase
Prior art date
Application number
HK15101956.3A
Other languages
English (en)
Chinese (zh)
Inventor
Christopher L. Rife
Derek H. Wells
Original Assignee
Danisco Us Inc.
The Goodyear Tire & Rubber Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc., The Goodyear Tire & Rubber Company filed Critical Danisco Us Inc.
Publication of HK1201554A1 publication Critical patent/HK1201554A1/xx

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03007—Pentalenene synthase (4.2.3.7)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L7/00—Compositions of natural rubber

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
HK15101956.3A 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene HK1201554A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161552453P 2011-10-27 2011-10-27
US201161552453P 2011-10-27
PCT/US2012/062308 WO2013063528A2 (en) 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene

Publications (1)

Publication Number Publication Date
HK1201554A1 true HK1201554A1 (en) 2015-09-04

Family

ID=47178953

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15101956.3A HK1201554A1 (en) 2011-10-27 2012-10-26 Isoprene synthase variants with improved solubility for production of isoprene

Country Status (8)

Country Link
US (2) US8735134B2 (https=)
EP (1) EP2771460B1 (https=)
JP (1) JP6101275B2 (https=)
BR (1) BR112014010053A8 (https=)
CA (1) CA2853125A1 (https=)
DK (1) DK2771460T3 (https=)
HK (1) HK1201554A1 (https=)
WO (1) WO2013063528A2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2337845A1 (en) * 2008-09-15 2011-06-29 Danisco US Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
JP6101275B2 (ja) 2011-10-27 2017-03-22 ダニスコ・ユーエス・インク イソプレン産生時の溶解度が向上されたイソプレン合成酵素変異体
WO2013096683A2 (en) * 2011-12-23 2013-06-27 Danisco Us Inc. Enhanced production of isoprene using marine bacterial cells
WO2014065271A1 (ja) * 2012-10-23 2014-05-01 積水化学工業株式会社 組換え細胞、並びに、イソプレンの生産方法
JPWO2015076392A1 (ja) 2013-11-22 2017-03-16 味の素株式会社 改変イソプレンシンターゼ
CN105985971B (zh) * 2015-02-10 2021-01-22 中国科学院微生物研究所 异戊二烯合成酶基因及其应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
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GB8407828D0 (en) 1984-03-27 1984-05-02 Ontario Research Foundation In situ preparation
US4570029A (en) 1985-03-04 1986-02-11 Uop Inc. Process for separating isoprene
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
US5849970A (en) 1995-06-23 1998-12-15 The Regents Of The University Of Colorado Materials and methods for the bacterial production of isoprene
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
AU2001278098A1 (en) 2000-07-31 2002-02-13 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
WO2004033646A2 (en) 2002-10-04 2004-04-22 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
US7947478B2 (en) 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
US20090076743A1 (en) 2007-08-01 2009-03-19 Drexel University Novel method for zero point detection
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
AU2009240505B2 (en) * 2008-04-23 2013-09-05 Danisco Us Inc. Isoprene synthase variants for improved microbial production of isoprene
CA2729302A1 (en) 2008-06-30 2010-01-14 The Goodyear Tire & Rubber Company Polymers of isoprene from renewable resources
MX318543B (es) 2008-07-02 2014-03-18 Danisco Us Inc Composiciones y metodos para producir isopreno libre de hidrocarburos de c5 bajo condicion de desacoplamiento y/o intervalos de operacion seguros.
EP2337845A1 (en) 2008-09-15 2011-06-29 Danisco US Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
ZA201102111B (en) 2008-09-15 2013-10-30 Danisco Us Inc Reduction of carbon dioxide emission during isoprene production by fermentation
CA2737195A1 (en) 2008-09-15 2010-03-18 The Goodyear Tire & Rubber Company Conversion of prenyl derivatives to isoprene
SG169641A1 (en) 2008-09-15 2011-04-29 Danisco Us Inc Systems using cell culture for production of isoprene
CA2737158A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
SG172806A1 (en) 2008-12-30 2011-08-29 Danisco Us Inc Methods of producing isoprene and a co-product
AU2010238770A1 (en) 2009-04-23 2011-11-03 Danisco Us Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201120213A (en) 2009-06-17 2011-06-16 Danisco Us Inc Polymerization of isoprene from renewable resources
TWI427149B (zh) 2009-06-17 2014-02-21 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
CA2783976A1 (en) 2009-12-18 2011-06-23 Danisco Us Inc. Purification of isoprene from renewable resources
EP2516657A1 (en) 2009-12-22 2012-10-31 Danisco US Inc. Membrane bioreactor for increased production of isoprene gas
EP2857518A3 (en) 2009-12-23 2015-04-22 Danisco US Inc. Compositions and methods of PGL for the increased production of isoprene
CA2816306A1 (en) * 2010-10-27 2012-05-03 Danisco Us Inc. Isoprene synthase variants for improved production of isoprene
JP6101275B2 (ja) 2011-10-27 2017-03-22 ダニスコ・ユーエス・インク イソプレン産生時の溶解度が向上されたイソプレン合成酵素変異体

Also Published As

Publication number Publication date
WO2013063528A2 (en) 2013-05-02
US9273299B2 (en) 2016-03-01
EP2771460B1 (en) 2017-04-26
BR112014010053A2 (pt) 2017-06-13
DK2771460T3 (en) 2017-06-06
EP2771460A2 (en) 2014-09-03
BR112014010053A8 (pt) 2017-06-20
US20130295632A1 (en) 2013-11-07
JP2015503904A (ja) 2015-02-05
US20140315275A1 (en) 2014-10-23
JP6101275B2 (ja) 2017-03-22
WO2013063528A3 (en) 2013-06-20
CA2853125A1 (en) 2013-05-02
US8735134B2 (en) 2014-05-27

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