HK1199048A1 - Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase - Google Patents

Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase Download PDF

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Publication number
HK1199048A1
HK1199048A1 HK14112310.2A HK14112310A HK1199048A1 HK 1199048 A1 HK1199048 A1 HK 1199048A1 HK 14112310 A HK14112310 A HK 14112310A HK 1199048 A1 HK1199048 A1 HK 1199048A1
Authority
HK
Hong Kong
Prior art keywords
isoprene
acetoacetyl
coa
production
isoprenoids
Prior art date
Application number
HK14112310.2A
Other languages
English (en)
Chinese (zh)
Inventor
Ilana S. Aldor
Zachary Q. Beck
Michael C. Miller
Caroline M. Peres
Original Assignee
Danisco Us Inc.
The Goodyear Tire & Rubber Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc., The Goodyear Tire & Rubber Company filed Critical Danisco Us Inc.
Publication of HK1199048A1 publication Critical patent/HK1199048A1/xx

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F36/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds
    • C08F36/02—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds
    • C08F36/04—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds conjugated
    • C08F36/08—Isoprene
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01194—Acetoacetyl-CoA synthase (2.3.1.194)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
HK14112310.2A 2011-08-04 2012-08-03 Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase HK1199048A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161515300P 2011-08-04 2011-08-04
US201161515300P 2011-08-04
PCT/US2012/049659 WO2013020118A1 (en) 2011-08-04 2012-08-03 Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase

Publications (1)

Publication Number Publication Date
HK1199048A1 true HK1199048A1 (en) 2015-06-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
HK14112310.2A HK1199048A1 (en) 2011-08-04 2012-08-03 Production of isoprene, isoprenoid precursors, and isoprenoids using acetoacetyl-coa synthase

Country Status (10)

Country Link
US (1) US20130122562A1 (enExample)
EP (1) EP2739658A1 (enExample)
JP (1) JP2014528705A (enExample)
CN (1) CN104039844A (enExample)
AU (1) AU2012289886A1 (enExample)
BR (1) BR112014002661A2 (enExample)
CA (1) CA2844064A1 (enExample)
HK (1) HK1199048A1 (enExample)
SG (1) SG2014007991A (enExample)
WO (1) WO2013020118A1 (enExample)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9422578B2 (en) 2011-06-17 2016-08-23 Invista North America S.A.R.L. Methods for biosynthesizing 1,3 butadiene
US9663801B2 (en) 2011-06-17 2017-05-30 Invista North America S.A.R.L. Methods of producing four carbon molecules
DK2776571T3 (en) 2011-11-09 2017-06-26 Amyris Inc PREPARATION OF ACETYL-COENZYM A-DERIVED ISOPRENOIDS
EP2794851A2 (en) * 2011-12-23 2014-10-29 Danisco US Inc. Enhanced production of isoprene using marine bacterial cells
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
BR112015002940A2 (pt) 2012-08-10 2018-04-24 Opx Biotechnologies Inc microorganismos e métodos para a produção de ácidos graxos e produtos derivados de ácidos graxos.
CN104903455A (zh) 2012-11-28 2015-09-09 英威达技术有限责任公司 用于异丁烯生物合成的方法
WO2014106122A1 (en) * 2012-12-31 2014-07-03 Genomatica, Inc. Compositions and methods for bio-butadiene production screening
US9447438B2 (en) 2013-03-15 2016-09-20 Cargill, Incorporated Acetyl-coA carboxylases
WO2015010103A2 (en) * 2013-07-19 2015-01-22 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
US10294496B2 (en) 2013-07-19 2019-05-21 Invista North America S.A.R.L. Methods for biosynthesizing 1,3 butadiene
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN105683385A (zh) * 2013-08-05 2016-06-15 英威达技术有限责任公司 用于生物合成异戊二烯的方法
AU2014306074B2 (en) * 2013-08-05 2018-08-30 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
EP3030668A1 (en) 2013-08-05 2016-06-15 Invista Technologies S.A R.L. Methods for biosynthesis of isobutene
US10648004B2 (en) 2014-02-20 2020-05-12 Danisco Us Inc. Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-CoA-derived products
CN106795519A (zh) 2014-06-16 2017-05-31 英威达技术有限责任公司 用于生成戊二酸和戊二酸甲酯的方法
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2017131488A1 (ko) * 2016-01-26 2017-08-03 경상대학교산학협력단 이소프렌의 생산 방법
KR101936825B1 (ko) * 2016-01-26 2019-01-11 경상대학교산학협력단 이소프렌의 생산 방법
JP2018014960A (ja) * 2016-07-29 2018-02-01 横浜ゴム株式会社 発現ベクター、形質転換された藻類、形質転換された珪藻、及び、ポリヌクレオチド
CN107815424B (zh) * 2016-09-12 2021-03-05 华东理工大学 一种产柠檬烯的解脂耶氏酵母基因工程菌及其应用
KR20190100386A (ko) 2017-01-06 2019-08-28 그린라이트 바이오사이언시스, 아이엔씨. 당의 무세포 생산
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞
CN107118988B (zh) * 2017-05-17 2020-11-24 重庆理工大学 高效复合沼气发酵催化剂
WO2019006257A1 (en) 2017-06-30 2019-01-03 Invista North America .S.A.R.L. METHODS, SYNTHETIC HOSTS AND REAGENTS FOR HYDROCARBON BIOSYNTHESIS
US11634733B2 (en) 2017-06-30 2023-04-25 Inv Nylon Chemicals Americas, Llc Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof
US11505809B2 (en) 2017-09-28 2022-11-22 Inv Nylon Chemicals Americas Llc Organisms and biosynthetic processes for hydrocarbon synthesis
CN109797173B (zh) * 2019-03-27 2022-09-20 山东泓达生物科技有限公司 一种β-法尼烯的生产方法
US20230090600A1 (en) * 2021-08-24 2023-03-23 Lanzatech, Inc. Microbial fermentation for the production of isoprenoid alcohols and derivatives
CN115820527B (zh) * 2022-12-09 2024-03-19 天津大学 一种生产甲羟戊酸的重组盐单胞菌及构建方法与应用
WO2024215256A2 (en) * 2023-04-11 2024-10-17 Allozymes Pte Ltd Methods and compositions
CN116925991B (zh) * 2023-07-28 2024-08-09 天津大学 高产甲羟戊酸的重组盐单胞菌菌株及构建方法与应用
CN117965414B (zh) * 2024-04-01 2024-06-14 北京微构工场生物技术有限公司 一种重组盐单胞菌及其在生产异戊二烯中的应用
CN118773228A (zh) * 2024-06-25 2024-10-15 广州新鑫生物科技有限公司 一种改造大肠杆菌代谢通路以提高乙酰辅酶a合成效率的方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US20050287655A1 (en) 2002-05-10 2005-12-29 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
CA2651747C (en) 2006-05-26 2017-10-24 Amyris Biotechnologies, Inc. Production of isoprenoids
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
BRPI0823506A8 (pt) 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
WO2009100231A2 (en) 2008-02-06 2009-08-13 The Regents Of The University Of California Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi
JP2011518564A (ja) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
US7632995B2 (en) 2008-05-09 2009-12-15 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH852179
EP2310490B1 (en) 2008-07-02 2019-01-02 Danisco US Inc. Methods for producing isoprene
HU3516U (en) 2008-07-31 2008-11-28 Zoltan Kuthi Anti-theft protection of draining for fuel tanks
BRPI0918936A2 (pt) 2008-09-15 2019-09-24 Danisco Us Inc conversão de derivados de prenila em isopreno
CA2737223A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
MY156562A (en) 2008-09-15 2016-02-26 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
WO2010031079A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Systems using cell culture for production of isoprene
CN102333866B (zh) 2008-12-30 2015-04-29 丹尼斯科美国公司 生产异戊二烯和共同产物的方法
US20100297749A1 (en) 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
EP2421966A2 (en) 2009-04-23 2012-02-29 Danisco US Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
CA2773675A1 (en) 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CA2785530A1 (en) * 2009-12-22 2011-06-23 Danisco Us Inc. Membrane bioreactor for increased production of isoprene gas
AU2010336342B2 (en) * 2009-12-23 2015-02-26 Danisco Us Inc. Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL)
US9309543B2 (en) * 2010-03-18 2016-04-12 William Marsh Rice University Bacteria and method for synthesizing fatty acids
JP2014502148A (ja) 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体

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Publication number Publication date
BR112014002661A2 (pt) 2019-09-24
CA2844064A1 (en) 2013-02-07
US20130122562A1 (en) 2013-05-16
WO2013020118A1 (en) 2013-02-07
EP2739658A1 (en) 2014-06-11
AU2012289886A1 (en) 2014-02-20
SG2014007991A (en) 2014-03-28
JP2014528705A (ja) 2014-10-30
CN104039844A (zh) 2014-09-10

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