HK1170010A1 - 生物學活性核酸酶的快速篩選以及經核酸酶修飾的細胞的分離 - Google Patents

生物學活性核酸酶的快速篩選以及經核酸酶修飾的細胞的分離

Info

Publication number
HK1170010A1
HK1170010A1 HK12110726.6A HK12110726A HK1170010A1 HK 1170010 A1 HK1170010 A1 HK 1170010A1 HK 12110726 A HK12110726 A HK 12110726A HK 1170010 A1 HK1170010 A1 HK 1170010A1
Authority
HK
Hong Kong
Prior art keywords
nuclease
isolation
biologically active
modified cells
rapid screening
Prior art date
Application number
HK12110726.6A
Other languages
English (en)
Inventor
Michael C Holmes
Tianjian Li
Original Assignee
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sangamo Biosciences Inc filed Critical Sangamo Biosciences Inc
Publication of HK1170010A1 publication Critical patent/HK1170010A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK12110726.6A 2009-06-30 2012-10-26 生物學活性核酸酶的快速篩選以及經核酸酶修飾的細胞的分離 HK1170010A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26987109P 2009-06-30 2009-06-30
PCT/US2010/001858 WO2011002503A1 (en) 2009-06-30 2010-06-29 Rapid screening of biologically active nucleases and isolation of nuclease-modified cells

Publications (1)

Publication Number Publication Date
HK1170010A1 true HK1170010A1 (zh) 2013-02-15

Family

ID=43411343

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12110726.6A HK1170010A1 (zh) 2009-06-30 2012-10-26 生物學活性核酸酶的快速篩選以及經核酸酶修飾的細胞的分離

Country Status (7)

Country Link
US (4) US20110014616A1 (zh)
EP (1) EP2449135B1 (zh)
JP (1) JP5798116B2 (zh)
AU (1) AU2010266705B2 (zh)
CA (1) CA2765488C (zh)
HK (1) HK1170010A1 (zh)
WO (1) WO2011002503A1 (zh)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
CN103025344B (zh) 2010-05-17 2016-06-29 桑格摩生物科学股份有限公司 新型dna-结合蛋白及其用途
EP2392208B1 (en) * 2010-06-07 2016-05-04 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Fusion proteins comprising a DNA-binding domain of a Tal effector protein and a non-specific cleavage domain of a restriction nuclease and their use
JP2013534417A (ja) * 2010-06-14 2013-09-05 アイオワ ステート ユニバーシティ リサーチ ファウンデーション，インコーポレーティッド Ｔａｌエフェクターとｆｏｋｉの融合タンパク質のヌクレアーゼ活性
US20120204282A1 (en) * 2011-02-04 2012-08-09 Sangamo Biosciences, Inc. Methods and compositions for treating occular disorders
KR20120096395A (ko) 2011-02-22 2012-08-30 주식회사 툴젠 뉴클레아제에 의해 유전자 변형된 세포를 농축시키는 방법
EP2734621B1 (en) * 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
EP2780460B1 (en) 2011-11-16 2018-07-11 Sangamo Therapeutics, Inc. Modified dna-binding proteins and uses thereof
US9688997B2 (en) 2011-12-29 2017-06-27 Iowa State University Research Foundation, Inc. Genetically modified plants with resistance to Xanthomonas and other bacterial plant pathogens
US9163284B2 (en) * 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
KR102530914B1 (ko) 2013-08-16 2023-05-11 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9840699B2 (en) 2013-12-12 2017-12-12 President And Fellows Of Harvard College Methods for nucleic acid editing
WO2015095804A1 (en) * 2013-12-19 2015-06-25 Amyris, Inc. Methods for genomic integration
CA2956224A1 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
SG10201903912XA (en) 2014-10-31 2019-05-30 Massachusetts Inst Technology Delivery of biomolecules to immune cells
US11125739B2 (en) 2015-01-12 2021-09-21 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
WO2016132122A1 (en) * 2015-02-17 2016-08-25 University Of Edinburgh Assay construct
EP3320082B1 (en) 2015-07-09 2023-05-24 Massachusetts Institute of Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
US10167457B2 (en) 2015-10-23 2019-01-01 President And Fellows Of Harvard College Nucleobase editors and uses thereof
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
WO2018136673A1 (en) * 2017-01-18 2018-07-26 Altius Institute For Biomedical Sciences Multiplexed screening
JP7236398B2 (ja) 2017-02-15 2023-03-09 ２セブンティ バイオ インコーポレイテッド ドナー修復鋳型多重ゲノム編集
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
US11268082B2 (en) 2017-03-23 2022-03-08 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
EP3658573A1 (en) 2017-07-28 2020-06-03 President and Fellows of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
BR112021018607A2 (pt) 2019-03-19 2021-11-23 Massachusetts Inst Technology Métodos e composições para editar sequências de nucleotídeos
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024106448A1 (ja) * 2022-11-18 2024-05-23 公立大学法人横浜市立大学 ミスマッチ修復欠損細胞を選択的に検出又は殺傷可能な核酸構築物及びその利用
WO2024163905A1 (en) 2023-02-03 2024-08-08 Genzyme Corporation Hsc-specific antibody conjugated lipid nanoparticles and uses thereof

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9011A (en) * 1852-06-15 Improvement
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
US5420032A (en) * 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5792632A (en) * 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
DE69534629D1 (de) 1994-01-18 2005-12-29 Scripps Research Inst Derivate von zinkfingerproteinen und methoden
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6242568B1 (en) * 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
ATE407205T1 (de) * 1994-08-20 2008-09-15 Gendaq Ltd Verbesserung in bezug auf bindungsproteine bei der erkennung von dna
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
FI105922B (fi) * 1995-04-06 2000-10-31 Fortum Oil & Gas Oy Termoplastinen biohajoava polyesteri, menetelmä sen valmistamiseksi ja siitä valmistetut tuotteet
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6534242B2 (en) 1997-11-06 2003-03-18 Canon Kabushiki Kaisha Multiple exposure device formation
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
DE69942334D1 (de) 1998-03-02 2010-06-17 Massachusetts Inst Technology Poly-zinkfinger-proteine mit verbesserten linkern
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7013219B2 (en) 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
US6196012B1 (en) * 1999-03-26 2001-03-06 Carrier Corporation Generator power management
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
US20030044787A1 (en) 2000-05-16 2003-03-06 Joung J. Keith Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
AU2002336373A1 (en) 2001-08-20 2003-03-03 The Scripps Research Institute Zinc finger binding domains for cnn
JP4968498B2 (ja) * 2002-01-23 2012-07-04 ユニバーシティ オブ ユタ リサーチ ファウンデーション ジンクフィンガーヌクレアーゼを用いる、標的化された染色体変異誘発
EP1485475B2 (en) * 2002-03-15 2017-09-20 Cellectis Hybrid and single chain meganucleases and use thereof
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
JP2006502748A (ja) 2002-09-05 2006-01-26 カリフォルニア インスティテュート オブ テクノロジー 遺伝子ターゲッティングを誘発するキメラヌクレアーゼの使用方法
AU2004208031B2 (en) * 2003-01-28 2009-10-08 Cellectis Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof.
KR20060039019A (ko) 2003-08-08 2006-05-04 상가모 바이오사이언스 인코포레이티드 표적화된 절단과 재조합을 위한 방법 및 그 조성물
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) * 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7972854B2 (en) * 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20060063231A1 (en) 2004-09-16 2006-03-23 Sangamo Biosciences, Inc. Compositions and methods for protein production
ES2347684T3 (es) * 2005-03-15 2010-11-03 Cellectis Variantes de la meganucleasa i-crei con especificidad modifica, metodo de preparacion y usos de las mismas.
MX2007013757A (es) 2005-05-05 2008-01-24 Univ Arizona Reagrupacion permitida de secuencia (seer)-met odo de novedad para visualizar secuencias de adn especificas.
CA2615532C (en) 2005-07-26 2016-06-28 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
DK2650365T3 (en) * 2005-10-18 2016-12-05 Prec Biosciences RATIONAL MEGANUCLEASES constructed with altered sequence specificity and DNA binding affinity
WO2007049095A1 (en) * 2005-10-25 2007-05-03 Cellectis Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof
JP5551432B2 (ja) * 2006-05-25 2014-07-16 サンガモ バイオサイエンシーズ， インコーポレイテッド 遺伝子不活性化のための方法と組成物
DE602007005634D1 (de) * 2006-05-25 2010-05-12 Sangamo Biosciences Inc Variante foki-spaltungshälften-domänen
EP2188384B1 (en) * 2007-09-27 2015-07-15 Sangamo BioSciences, Inc. Rapid in vivo identification of biologically active nucleases
CA2726768C (en) * 2008-06-10 2016-09-06 Sangamo Biosciences, Inc. Methods and compositions for generation of bax- and bak-deficient cell lines
EP2206723A1 (en) * 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
JP2013513389A (ja) * 2009-12-10 2013-04-22 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ Ｔａｌエフェクターに媒介されるｄｎａ修飾

Also Published As

Publication number Publication date
JP5798116B2 (ja) 2015-10-21
EP2449135A1 (en) 2012-05-09
US20120237926A1 (en) 2012-09-20
EP2449135A4 (en) 2012-11-28
CA2765488A1 (en) 2011-01-06
US20130171657A1 (en) 2013-07-04
US20110244474A1 (en) 2011-10-06
US9115409B2 (en) 2015-08-25
US9121072B2 (en) 2015-09-01
CA2765488C (en) 2018-01-02
JP2012531909A (ja) 2012-12-13
AU2010266705A1 (en) 2012-01-12
US20110014616A1 (en) 2011-01-20
EP2449135B1 (en) 2016-01-06
AU2010266705B2 (en) 2014-06-05
WO2011002503A1 (en) 2011-01-06

Similar Documents

Publication Publication Date Title
HK1170010A1 (zh) 生物學活性核酸酶的快速篩選以及經核酸酶修飾的細胞的分離
IL261851B (en) Reprogramming of t cells and hematopoietic cells
IL267629A (en) A method for eukaryotic cell culture
GB2458863B (en) Improved culture of stem cells
ZA201201811B (en) Planar antenna array and article of manufacture using same
GB0823074D0 (en) Design and method of assembly of cdi cells
HK1170258A1 (zh) 細胞的擴增
ZA201008392B (en) Treatment of pluripotent cells
HK1138374A1 (en) Methods of evaluating cells and cell cultures
ZA201104349B (en) Pharmaceutical preparation comprising supernatant of blood mononuclear cell culture
EP2408899A4 (en) DEVICES FOR SEPARATING CELLS AND METHODS FOR USING SAME
EP2485678A4 (en) COFERONS AND METHODS OF MAKING AND USING THE SAME
EP2383818A4 (en) ELECTROCHEMICAL DEVICE AND METHOD FOR MANUFACTURING THE SAME
EP2393930A4 (en) CELLULAR LINES EXPRESSING CFTR AND METHODS OF USE THEREOF
GB0911331D0 (en) Characterising properties or behaviour of biological cells
EP2525867A4 (en) ELECTRODE ARRAY AND METHOD FOR THE PRODUCTION THEREOF
EP2446966A4 (en) ROW OF COVERS AND MICROTUBE ROW ASSEMBLY COMPRISING SAME
EP2481112A4 (en) MANGANPHOSPHATES AND ELECTRODE-ACTIVE MATERIALS THEREFOR
SG10201405455UA (en) Cells and methods for obtaining them
PT2233560T (pt) Dispositivo de cultura de células ou tecidos e conjunto associado
HK1143892A1 (en) An active solar cell and method of manufacture
GB0723504D0 (en) Treatment of eukaryotic cellular biomass
GB201002469D0 (en) Parthenote-derived stem cells and methods of making and using them
EP2391710A4 (en) CELL LINES EXPRESSING GUANYLATE CYCLASE-C AND METHODS OF USE THEREOF
EP2460542A4 (en) ENDOKRINER PANCREAS CELL INDICATOR AND ITS USE