GB833973A - Purification of the antibiotic ristocetin - Google Patents

Purification of the antibiotic ristocetin

Info

Publication number
GB833973A
GB833973A GB26700/58A GB2670058A GB833973A GB 833973 A GB833973 A GB 833973A GB 26700/58 A GB26700/58 A GB 26700/58A GB 2670058 A GB2670058 A GB 2670058A GB 833973 A GB833973 A GB 833973A
Authority
GB
United Kingdom
Prior art keywords
ristocetin
aldehyde
aqueous
addition
compound
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB26700/58A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of GB833973A publication Critical patent/GB833973A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
    • C07D311/70—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with two hydrocarbon radicals attached in position 2 and elements other than carbon and hydrogen in position 6
    • C07D311/72—3,4-Dihydro derivatives having in position 2 at least one methyl radical and in position 6 one oxygen atom, e.g. tocopherols

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Ristocetin, ristocetin A and ristocetin B, are purified by adding an aromatic aldehyde to an aqueous solution of the said antibiotic in order to precipitate the compound of ristocetin with the aldehyde. The aqueous solution, which may be a clarified broth, is preferably adjusted to pH 7.0 to 8.1 and especially 7.5 to 8.1 by the addition of alkali such as sodium bicarbonate or sodium hydroxide prior to the addition of the aldehyde which is preferably salicyl-aldehyde or cinnamaldehyde. The compound contains three aromatic aldehyde groups per mol. of ristocetin and in the case of the salicyl-aldehyde-ristocetin A is soluble in polyethylene glycol, propylene glycol, aqueous acetone, aqueous methanol, aqueous ethanol, pyridine and formamide and isoluble in amyl acetate, ethyl acetate, benzene and chloroform. It is non-irritating to tissue in 50% aqueous methanol. Ristocetin is recovered from the aldehyde compound by acidification, e.g. to pH 2.0 and (a) extracting the freed aromatic aldehyde with a solvent such as chloroform, or (b) precipitating the ristocetin salt, e.g. the sulphate by the addition of a non-solvent such as acetone. Specification 765,880 is referred to.
GB26700/58A 1957-08-20 1958-08-19 Purification of the antibiotic ristocetin Expired GB833973A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US833973XA 1957-08-20 1957-08-20

Publications (1)

Publication Number Publication Date
GB833973A true GB833973A (en) 1960-05-04

Family

ID=22178027

Family Applications (1)

Application Number Title Priority Date Filing Date
GB26700/58A Expired GB833973A (en) 1957-08-20 1958-08-19 Purification of the antibiotic ristocetin

Country Status (1)

Country Link
GB (1) GB833973A (en)

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