GB829266A - A method of manufacturing bacteriophages in solid dry form - Google Patents

Abstract

A therapeutic bacteriophage preparation in solid dry form is obtained by cultivating a strain of a pathogenic bacteria by submerged aerobic cultivation in an aqueous solution of a protein hydrolysate, effecting lysis of the bacteria by the corresponding bacteriophage, precipitating the bacteriophage from the lysate by means of an inorganic salt, e.g. ammonium sulphate, and drying the precipitate, preferably after admixture with 10% calcium gluconate. The solid is then pressed into tablets or filled into capsules which are rendered enteric by coating with graphite or acetylphthalyl cellulose. For parenteral use, the concentrate is desalted by dialysis. The aqueous solution of protein hydrolipate may be an hydrolipate of beef, casein or soya bean containing 200 mg. per cent amine nitrogen. In the example for the preparation of Dysenteric Phage Sonne, the aqueous medium contains beef hydrolipate (200 mg. per cent amine nitrogen), 0.5% sodium chloride and 0.1% sodium monohydrogen phosphate of pH 7.6. After sterilization, it is inoculated with a suspension of Shigella sonnei and incubated 3 hours under submerged aerobic cultivation. It is then inoculated with Shigella sonnei phage and lipis allowed to continue until the culture clarifies and the total nitrogen in the medium is the same as at the beginning of the cultivation. The culture is heated to 56 DEG C. to inactivate the bacteria but not the phage, filtered, cooled to 0 DEG C. and ammonium sulphate added to saturation. 10% calcium gluconate is added to the web precipitate and the mixture dried in vacuo. Other therapeutic bacteriophages which may be made are those of typhus, paratyphus and other Salmonellas, Vibrio cholerae, E. coli, Staphylococcus and Proteus. They may be used for the treatment of man and animals.