GB2612412B - Systems and methods for targeted nucleic acid capture - Google Patents
Systems and methods for targeted nucleic acid captureInfo
- Publication number
- GB2612412B GB2612412B GB2211994.5A GB202211994A GB2612412B GB 2612412 B GB2612412 B GB 2612412B GB 202211994 A GB202211994 A GB 202211994A GB 2612412 B GB2612412 B GB 2612412B
- Authority
- GB
- United Kingdom
- Prior art keywords
- methods
- nucleic acid
- systems
- acid capture
- targeted nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6832—Enhancement of hybridisation reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062968847P | 2020-01-31 | 2020-01-31 | |
| US202062987232P | 2020-03-09 | 2020-03-09 | |
| US202062988859P | 2020-03-12 | 2020-03-12 | |
| PCT/US2021/016089 WO2021155374A2 (en) | 2020-01-31 | 2021-02-01 | Systems and methods for targeted nucleic acid capture |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB202211994D0 GB202211994D0 (en) | 2022-09-28 |
| GB2612412A GB2612412A (en) | 2023-05-03 |
| GB2612412B true GB2612412B (en) | 2025-10-10 |
Family
ID=77079995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2211994.5A Active GB2612412B (en) | 2020-01-31 | 2021-02-01 | Systems and methods for targeted nucleic acid capture |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20230193380A1 (https=) |
| EP (2) | EP4097231B1 (https=) |
| JP (2) | JP7732991B2 (https=) |
| KR (1) | KR20220133984A (https=) |
| CN (1) | CN115103909A (https=) |
| CA (1) | CA3162647A1 (https=) |
| GB (1) | GB2612412B (https=) |
| WO (1) | WO2021155374A2 (https=) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2022513124A (ja) * | 2018-11-21 | 2022-02-07 | アヴィダ バイオメッド， インコーポレイテッド | 標的化された核酸ライブラリー形成のための方法 |
| KR20240150780A (ko) * | 2022-02-18 | 2024-10-16 | 애질런트 테크놀로지스, 인크. | 표적 핵산 포획 및 바코딩을 위한 시스템 및 방법 |
| US20250369045A1 (en) * | 2022-12-20 | 2025-12-04 | Illumina, Inc. | Multivalent assemblies for enhanced target hybridization |
| CN116574780B (zh) * | 2023-02-24 | 2023-11-10 | 苏州唯善生物科技有限公司 | 一种单荧光通道同时检测两种miRNA的引物探针设计方法、试剂盒及定量检测方法 |
| WO2025221668A1 (en) * | 2024-04-19 | 2025-10-23 | Agilent Technologies, Inc. | Methods of preparing nucleic acids for sequencing and methylation analysis |
| WO2025221722A1 (en) * | 2024-04-19 | 2025-10-23 | Agilent Technologies, Inc. | Methods of selectively tagging nucleic acids for analysis of rna sequences |
| US12509733B1 (en) | 2025-04-07 | 2025-12-30 | Inivata Ltd. | Methods and systems for detecting a primary disease |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060183128A1 (en) | 2003-08-12 | 2006-08-17 | Epigenomics Ag | Methods and compositions for differentiating tissues for cell types using epigenetic markers |
| EP2053132A1 (en) * | 2007-10-23 | 2009-04-29 | Roche Diagnostics GmbH | Enrichment and sequence analysis of geomic regions |
| EP2334802A4 (en) * | 2008-09-09 | 2012-01-25 | Life Technologies Corp | METHODS OF GENERATING SPECIFIC LIBRARIES OF GENES |
| US20100167952A1 (en) * | 2008-11-06 | 2010-07-01 | Thomas Albert | Suppression of secondary capture in microarray assays |
| US20100331204A1 (en) * | 2009-02-13 | 2010-12-30 | Jeff Jeddeloh | Methods and systems for enrichment of target genomic sequences |
| EP2619329B1 (en) * | 2010-09-24 | 2019-05-22 | The Board of Trustees of The Leland Stanford Junior University | Direct capture, amplification and sequencing of target dna using immobilized primers |
| WO2014015098A1 (en) * | 2012-07-18 | 2014-01-23 | Siemens Healthcare Diagnostics Inc. | A method of normalizing biological samples |
| SI4056712T1 (sl) | 2012-09-20 | 2024-10-30 | The Chinese University Of Hong Kong | Neinvazivno določanje metiloma tumorja iz plazme |
| US20160340740A1 (en) | 2014-01-30 | 2016-11-24 | The Regents Of The University Of California | Methylation haplotyping for non-invasive diagnosis (monod) |
| WO2015159292A2 (en) | 2014-04-14 | 2015-10-22 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | A method and kit for determining the tissue or cell origin of dna |
| TWI813141B (zh) | 2014-07-18 | 2023-08-21 | 香港中文大學 | Ｄｎａ混合物中之組織甲基化模式分析 |
| CN109072300B (zh) | 2015-12-17 | 2023-01-31 | 伊路敏纳公司 | 区分复杂生物样品中的甲基化水平 |
| US11512344B2 (en) * | 2016-05-10 | 2022-11-29 | Arizona Board Of Regents On Behalf Of Arizona State University | Consecutive hybridization for multiplexed analysis of biological samples |
| EP3497242B1 (en) * | 2016-08-10 | 2025-01-22 | Grail, Inc. | Methods of analyzing nucleic acid fragments |
| ES3022457T3 (en) | 2016-12-21 | 2025-05-28 | Univ California | Deconvolution and detection of rare dna in plasma |
| WO2019241290A1 (en) * | 2018-06-12 | 2019-12-19 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
-
2021
- 2021-02-01 WO PCT/US2021/016089 patent/WO2021155374A2/en not_active Ceased
- 2021-02-01 EP EP21747390.9A patent/EP4097231B1/en active Active
- 2021-02-01 CN CN202180011915.0A patent/CN115103909A/zh active Pending
- 2021-02-01 EP EP25156820.0A patent/EP4534690A3/en active Pending
- 2021-02-01 KR KR1020227029940A patent/KR20220133984A/ko active Pending
- 2021-02-01 GB GB2211994.5A patent/GB2612412B/en active Active
- 2021-02-01 JP JP2022546396A patent/JP7732991B2/ja active Active
- 2021-02-01 CA CA3162647A patent/CA3162647A1/en active Pending
-
2022
- 2022-07-29 US US17/816,198 patent/US20230193380A1/en active Pending
-
2024
- 2024-04-30 US US18/651,023 patent/US20240287601A1/en active Pending
-
2025
- 2025-08-21 JP JP2025138504A patent/JP2025169409A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP7732991B2 (ja) | 2025-09-02 |
| CA3162647A1 (en) | 2021-08-05 |
| JP2025169409A (ja) | 2025-11-12 |
| WO2021155374A3 (en) | 2021-09-10 |
| EP4097231A4 (en) | 2024-04-03 |
| KR20220133984A (ko) | 2022-10-05 |
| GB2612412A (en) | 2023-05-03 |
| EP4097231C0 (en) | 2025-03-19 |
| EP4534690A3 (en) | 2025-07-23 |
| US20230193380A1 (en) | 2023-06-22 |
| US20240287601A1 (en) | 2024-08-29 |
| EP4534690A2 (en) | 2025-04-09 |
| EP4097231B1 (en) | 2025-03-19 |
| JP2023512522A (ja) | 2023-03-27 |
| WO2021155374A2 (en) | 2021-08-05 |
| CN115103909A (zh) | 2022-09-23 |
| GB202211994D0 (en) | 2022-09-28 |
| EP4097231A2 (en) | 2022-12-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GB2612412B (en) | Systems and methods for targeted nucleic acid capture | |
| MX2021003712A (es) | Reducción de arn basada en nucleasa. | |
| ATE514775T1 (de) | Endenmodifikation zur verhinderung einer überrepräsentierung von fragmenten | |
| EP4421185A3 (en) | Method for spatial tagging and analysing nucleic acids in a biological specimen | |
| MX2022000377A (es) | Analisis de metilacion del genoma completo sin bisulfito. | |
| MY195527A (en) | Methods And Systems For Tumor Detection | |
| WO2017065959A3 (en) | Methods and compositions that utilize transcriptome sequencing data in machine learning-based classification | |
| EP4512527A3 (en) | Microfluidic siphoning array for nucleic acid quantification | |
| MX2011007937A (es) | Produccion de adn lineal cerrado. | |
| EP4617381A3 (en) | Methods and systems for nucleic acid analysis and quantification | |
| EP4644568A3 (en) | Methods for targeted nucleic acid library formation | |
| ATE443777T1 (de) | Methoden und zusammensetzungen zur analyse von ribonukleinsäuren | |
| NZ701145A (en) | Compositions and methods for quantifying a nucleic acid sequence in a sample | |
| EA201170933A1 (ru) | Анализ нуклеиновых кислот, полученных из единичных клеток | |
| EP4219519A3 (en) | Competitive probes for engineering signal generation | |
| MX2015012319A (es) | Sistemas de instrumento analitico. | |
| PH12018502739B1 (en) | Method for producing dna library and method for analyzing genomic dna using the dna library | |
| WO2019122879A3 (en) | Methods for performing biological reactions | |
| BR112014014307A2 (pt) | métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica | |
| MY184632A (en) | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii | |
| MX2021009482A (es) | Metodos para el analisis de acidos nucleicos. | |
| BR112017000449A2 (pt) | processo para iniciar e realizar a conversão de co em um produto final, sistema, e, produto de programa de computador. | |
| NZ732886A (en) | Amplification and detection of nucleic acids in a biological sample | |
| UY38479A (es) | Método y producto informático de análisis de adn fetal por secuenciación masiva | |
| WO2015009844A3 (en) | Mirror bisulfite analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 732E | Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977) |
Free format text: REGISTERED BETWEEN 20230706 AND 20230712 |