JP7732991B2 - 標的化された核酸捕捉のためのシステムおよび方法 - Google Patents

標的化された核酸捕捉のためのシステムおよび方法

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Publication number
JP7732991B2
JP7732991B2 JP2022546396A JP2022546396A JP7732991B2 JP 7732991 B2 JP7732991 B2 JP 7732991B2 JP 2022546396 A JP2022546396 A JP 2022546396A JP 2022546396 A JP2022546396 A JP 2022546396A JP 7732991 B2 JP7732991 B2 JP 7732991B2
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probe
nucleic acid
sequence
adapter
template nucleic
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Japanese (ja)
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JP2023512522A5 (https=
JP2023512522A (ja
Inventor
シェンロン リン，
ユン バオ，
ヘン ワン，
グレイス ジャオ，
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Agilent Technologies Inc
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Agilent Technologies Inc
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Priority to JP2025138504A priority Critical patent/JP2025169409A/ja
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2022546396A 2020-01-31 2021-02-01 標的化された核酸捕捉のためのシステムおよび方法 Active JP7732991B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025138504A JP2025169409A (ja) 2020-01-31 2025-08-21 標的化された核酸捕捉のためのシステムおよび方法

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202062968847P 2020-01-31 2020-01-31
US62/968,847 2020-01-31
US202062987232P 2020-03-09 2020-03-09
US62/987,232 2020-03-09
US202062988859P 2020-03-12 2020-03-12
US62/988,859 2020-03-12
PCT/US2021/016089 WO2021155374A2 (en) 2020-01-31 2021-02-01 Systems and methods for targeted nucleic acid capture

Related Child Applications (1)

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Publications (3)

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JP2023512522A JP2023512522A (ja) 2023-03-27
JP2023512522A5 JP2023512522A5 (https=) 2024-02-07
JP7732991B2 true JP7732991B2 (ja) 2025-09-02

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JP2025138504A Pending JP2025169409A (ja) 2020-01-31 2025-08-21 標的化された核酸捕捉のためのシステムおよび方法

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US (2) US20230193380A1 (https=)
EP (2) EP4097231B1 (https=)
JP (2) JP7732991B2 (https=)
KR (1) KR20220133984A (https=)
CN (1) CN115103909A (https=)
CA (1) CA3162647A1 (https=)
GB (1) GB2612412B (https=)
WO (1) WO2021155374A2 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022513124A (ja) * 2018-11-21 2022-02-07 アヴィダ バイオメッド， インコーポレイテッド 標的化された核酸ライブラリー形成のための方法
KR20240150780A (ko) * 2022-02-18 2024-10-16 애질런트 테크놀로지스, 인크. 표적 핵산 포획 및 바코딩을 위한 시스템 및 방법
US20250369045A1 (en) * 2022-12-20 2025-12-04 Illumina, Inc. Multivalent assemblies for enhanced target hybridization
CN116574780B (zh) * 2023-02-24 2023-11-10 苏州唯善生物科技有限公司 一种单荧光通道同时检测两种miRNA的引物探针设计方法、试剂盒及定量检测方法
WO2025221668A1 (en) * 2024-04-19 2025-10-23 Agilent Technologies, Inc. Methods of preparing nucleic acids for sequencing and methylation analysis
WO2025221722A1 (en) * 2024-04-19 2025-10-23 Agilent Technologies, Inc. Methods of selectively tagging nucleic acids for analysis of rna sequences
US12509733B1 (en) 2025-04-07 2025-12-30 Inivata Ltd. Methods and systems for detecting a primary disease

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010030683A1 (en) 2008-09-09 2010-03-18 Rosetta Inpharmatics Llc Methods of generating gene specific libraries
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060183128A1 (en) 2003-08-12 2006-08-17 Epigenomics Ag Methods and compositions for differentiating tissues for cell types using epigenetic markers
EP2053132A1 (en) * 2007-10-23 2009-04-29 Roche Diagnostics GmbH Enrichment and sequence analysis of geomic regions
US20100167952A1 (en) * 2008-11-06 2010-07-01 Thomas Albert Suppression of secondary capture in microarray assays
US20100331204A1 (en) * 2009-02-13 2010-12-30 Jeff Jeddeloh Methods and systems for enrichment of target genomic sequences
EP2619329B1 (en) * 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
SI4056712T1 (sl) 2012-09-20 2024-10-30 The Chinese University Of Hong Kong Neinvazivno določanje metiloma tumorja iz plazme
US20160340740A1 (en) 2014-01-30 2016-11-24 The Regents Of The University Of California Methylation haplotyping for non-invasive diagnosis (monod)
WO2015159292A2 (en) 2014-04-14 2015-10-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. A method and kit for determining the tissue or cell origin of dna
TWI813141B (zh) 2014-07-18 2023-08-21 香港中文大學 Ｄｎａ混合物中之組織甲基化模式分析
CN109072300B (zh) 2015-12-17 2023-01-31 伊路敏纳公司 区分复杂生物样品中的甲基化水平
US11512344B2 (en) * 2016-05-10 2022-11-29 Arizona Board Of Regents On Behalf Of Arizona State University Consecutive hybridization for multiplexed analysis of biological samples
EP3497242B1 (en) * 2016-08-10 2025-01-22 Grail, Inc. Methods of analyzing nucleic acid fragments
ES3022457T3 (en) 2016-12-21 2025-05-28 Univ California Deconvolution and detection of rare dna in plasma

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010030683A1 (en) 2008-09-09 2010-03-18 Rosetta Inpharmatics Llc Methods of generating gene specific libraries
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products

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Publication number Publication date
CA3162647A1 (en) 2021-08-05
JP2025169409A (ja) 2025-11-12
WO2021155374A3 (en) 2021-09-10
EP4097231A4 (en) 2024-04-03
KR20220133984A (ko) 2022-10-05
GB2612412A (en) 2023-05-03
GB2612412B (en) 2025-10-10
EP4097231C0 (en) 2025-03-19
EP4534690A3 (en) 2025-07-23
US20230193380A1 (en) 2023-06-22
US20240287601A1 (en) 2024-08-29
EP4534690A2 (en) 2025-04-09
EP4097231B1 (en) 2025-03-19
JP2023512522A (ja) 2023-03-27
WO2021155374A2 (en) 2021-08-05
CN115103909A (zh) 2022-09-23
GB202211994D0 (en) 2022-09-28
EP4097231A2 (en) 2022-12-07

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