MX2022000377A - Analisis de metilacion del genoma completo sin bisulfito. - Google Patents

Analisis de metilacion del genoma completo sin bisulfito.

Info

Publication number
MX2022000377A
MX2022000377A MX2022000377A MX2022000377A MX2022000377A MX 2022000377 A MX2022000377 A MX 2022000377A MX 2022000377 A MX2022000377 A MX 2022000377A MX 2022000377 A MX2022000377 A MX 2022000377A MX 2022000377 A MX2022000377 A MX 2022000377A
Authority
MX
Mexico
Prior art keywords
bisulfite
free
whole genome
methylation analysis
dhu
Prior art date
Application number
MX2022000377A
Other languages
English (en)
Inventor
Chunxiao Song
Jingfei Cheng
Paulina Siejka-Zielinska
Yibin Liu
Original Assignee
Ludwig Inst For Cancer Res Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Inst For Cancer Res Ltd filed Critical Ludwig Inst For Cancer Res Ltd
Publication of MX2022000377A publication Critical patent/MX2022000377A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/115—Characterised by chemical treatment oxidising agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Esta descripción proporciona métodos para la identificación sin bisulfito económica en el ADN, incluido el ADN genómico completo, de las ubicaciones de una o más de 5-metilcitosina, 5-hidroximetilcitosina, 5-carboxilcitosina y 5-formilcitosina. Los métodos descritos en la presente se basan en la conversión de citosina modificada (5mC, 5hmC, 5fC, 5caC) en dihidrouracilo (DHU), por ejemplo, mediante tratamiento con piridina borano asistido por TET, seguido de escisión por endonucleasa del DHU, e identificación del sitio de escisión, que corresponde a la ubicación de la citosina modificada.
MX2022000377A 2019-07-08 2020-07-08 Analisis de metilacion del genoma completo sin bisulfito. MX2022000377A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962871444P 2019-07-08 2019-07-08
PCT/IB2020/056435 WO2021005537A1 (en) 2019-07-08 2020-07-08 Bisulfite-free, whole genome methylation analysis

Publications (1)

Publication Number Publication Date
MX2022000377A true MX2022000377A (es) 2022-03-25

Family

ID=71895032

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022000377A MX2022000377A (es) 2019-07-08 2020-07-08 Analisis de metilacion del genoma completo sin bisulfito.

Country Status (12)

Country Link
US (1) US20220275424A1 (es)
EP (2) EP3997245B1 (es)
JP (1) JP2022540453A (es)
KR (1) KR20220038367A (es)
CN (1) CN114174534A (es)
AU (1) AU2020310613A1 (es)
BR (1) BR112022000361A2 (es)
CA (1) CA3146212A1 (es)
ES (1) ES2969617T3 (es)
IL (1) IL289669A (es)
MX (1) MX2022000377A (es)
WO (1) WO2021005537A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023023500A1 (en) * 2021-08-17 2023-02-23 Illumina, Inc. Methods and compositions for identifying methylated cytosines
WO2023081722A2 (en) * 2021-11-02 2023-05-11 Guardant Health, Inc. Quality control method
CN114085894A (zh) * 2021-11-03 2022-02-25 翌圣生物科技(上海)股份有限公司 核酸甲基化胞嘧啶转化方法
WO2023107453A1 (en) * 2021-12-06 2023-06-15 Chan Zuckerberg Biohub, Inc. Method for combined genome methylation and variation analyses
WO2023187012A1 (en) * 2022-03-31 2023-10-05 Illumina Cambridge Limited Compositions including aqueous amine borane complexes and polynucleotides, and methods of using the same to detect methylcytosine or hydroxymethylcytosine
CN115161400B (zh) * 2022-06-16 2023-06-20 温州医科大学 一种dna甲基化检测方法及所用试剂
EP4296372A1 (en) * 2022-06-22 2023-12-27 Universitat Pompeu Fabra (UPF) Method to detect and discriminate cytosine modifications
CN115820580A (zh) * 2022-08-31 2023-03-21 武汉爱博泰克生物科技有限公司 活性重组hTET2蛋白的表达及纯化方法
WO2024073047A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024076981A2 (en) * 2022-10-04 2024-04-11 Exact Sciences Corporation Tet-assisted pyridine borane sequencing
CN115404270B (zh) * 2022-10-31 2023-01-31 臻和(北京)生物科技有限公司 一种dna甲基化测序文库甲基化转化率的评估方法、应用、终端设备及存储介质

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008091541A1 (en) * 2007-01-22 2008-07-31 University Of Vermont And State Agricultural College Molecular accessibility assay
AU2012291873B2 (en) 2011-07-29 2016-03-10 Cambridge Epigenetix Limited Methods for detection of nucleotide modification
WO2014165770A1 (en) 2013-04-05 2014-10-09 The University Of Chicago Single-base resolution sequencing of 5-formylcytosine (5fc) and 5-carboxylcytosine (5cac)
EP3280793A4 (en) * 2015-04-06 2018-10-03 The Regents of the University of California Methods for determining base locations in a polynucleotide
JPWO2017039002A1 (ja) 2015-09-04 2018-08-30 国立大学法人 東京大学 ５−ヒドロキシメチルシトシン酸化剤及び５−ヒドロキシメチルシトシン解析方法
SG11202006511YA (en) * 2018-01-08 2020-08-28 Ludwig Inst For Cancer Res Ltd Bisulfite-free, base-resolution identification of cytosine modifications
WO2024076981A2 (en) * 2022-10-04 2024-04-11 Exact Sciences Corporation Tet-assisted pyridine borane sequencing

Also Published As

Publication number Publication date
CA3146212A1 (en) 2021-01-14
BR112022000361A2 (pt) 2022-05-10
EP3997245A1 (en) 2022-05-18
EP4306652A3 (en) 2024-03-27
JP2022540453A (ja) 2022-09-15
EP4306652A2 (en) 2024-01-17
AU2020310613A1 (en) 2022-02-17
CN114174534A (zh) 2022-03-11
EP3997245B1 (en) 2023-10-18
US20220275424A1 (en) 2022-09-01
IL289669A (en) 2022-03-01
KR20220038367A (ko) 2022-03-28
WO2021005537A1 (en) 2021-01-14
ES2969617T3 (es) 2024-05-21

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