GB2163453A - Vessel for culturing cells on microcarriers or in capsules - Google Patents
Vessel for culturing cells on microcarriers or in capsules Download PDFInfo
- Publication number
- GB2163453A GB2163453A GB08520929A GB8520929A GB2163453A GB 2163453 A GB2163453 A GB 2163453A GB 08520929 A GB08520929 A GB 08520929A GB 8520929 A GB8520929 A GB 8520929A GB 2163453 A GB2163453 A GB 2163453A
- Authority
- GB
- United Kingdom
- Prior art keywords
- vessel
- cells
- microcarriers
- volume
- output port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000012258 culturing Methods 0.000 title claims abstract description 15
- 239000002775 capsule Substances 0.000 title abstract description 14
- 210000004102 animal cell Anatomy 0.000 claims abstract description 42
- 210000004027 cell Anatomy 0.000 claims abstract description 41
- 239000012530 fluid Substances 0.000 claims abstract description 25
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 230000001133 acceleration Effects 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims description 36
- 239000012528 membrane Substances 0.000 claims description 11
- 238000004113 cell culture Methods 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 7
- 230000006378 damage Effects 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 210000004379 membrane Anatomy 0.000 claims 3
- 238000011065 in-situ storage Methods 0.000 abstract description 6
- 239000000725 suspension Substances 0.000 abstract description 2
- 238000012216 screening Methods 0.000 abstract 1
- 239000003094 microcapsule Substances 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 229910052760 oxygen Inorganic materials 0.000 description 8
- 239000001301 oxygen Substances 0.000 description 8
- 239000000661 sodium alginate Substances 0.000 description 8
- 235000010413 sodium alginate Nutrition 0.000 description 8
- 229940005550 sodium alginate Drugs 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 239000007789 gas Substances 0.000 description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 210000001822 immobilized cell Anatomy 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 108010039918 Polylysine Proteins 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000000648 calcium alginate Substances 0.000 description 2
- 235000010410 calcium alginate Nutrition 0.000 description 2
- 229960002681 calcium alginate Drugs 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 210000004408 hybridoma Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 229920000656 polylysine Polymers 0.000 description 2
- 229920002714 polyornithine Polymers 0.000 description 2
- 108010055896 polyornithine Proteins 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000219289 Silene Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002844 continuous effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012526 feed medium Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 102000035122 glycosylated proteins Human genes 0.000 description 1
- 108091005608 glycosylated proteins Proteins 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US64419184A | 1984-08-24 | 1984-08-24 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB8520929D0 GB8520929D0 (en) | 1985-09-25 |
| GB2163453A true GB2163453A (en) | 1986-02-26 |
Family
ID=24583842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB08520929A Withdrawn GB2163453A (en) | 1984-08-24 | 1985-08-21 | Vessel for culturing cells on microcarriers or in capsules |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JPS61108371A (enrdf_load_stackoverflow) |
| DE (1) | DE3529203A1 (enrdf_load_stackoverflow) |
| GB (1) | GB2163453A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4943535A (en) * | 1985-06-20 | 1990-07-24 | Celltech Limited | Anti-lift fermenter |
| WO2014110512A1 (en) * | 2013-01-11 | 2014-07-17 | Pbs Biotech, Inc. | Method and apparatus for the use of micro-carriers in a disposable bioreactor system |
| WO2019002608A1 (fr) * | 2017-06-30 | 2019-01-03 | Universite Paris Diderot Paris 7 | Systeme fluidique de production de vesicules extracellulaires et procede associe |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0628595B2 (ja) * | 1985-03-29 | 1994-04-20 | 株式会社日立製作所 | 培養装置 |
| JPS6423888A (en) * | 1987-07-16 | 1989-01-26 | Etsuko Kakizaki | Culture vessel with micro-cellular wall |
| DE102008039812A1 (de) * | 2008-08-25 | 2010-03-04 | Yvonne Ibold | Vorrichtung und Verfahren zur Kultivierung von adhärenten Zellen |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS531828B2 (enrdf_load_stackoverflow) * | 1974-03-27 | 1978-01-23 | ||
| US4184916A (en) * | 1977-11-14 | 1980-01-22 | Monsanto Company | Continuous cell culture system |
| JPS559751A (en) * | 1978-07-06 | 1980-01-23 | Nakajima Seisakusho:Kk | Agitation and fermentation equipment |
| AU6156780A (en) * | 1979-08-24 | 1981-04-09 | G.D. Searle & Co. | Stack plate culture |
| US4355906A (en) * | 1981-04-03 | 1982-10-26 | Bellco Glass Inc. | Stirring apparatus for cell culture |
-
1985
- 1985-08-14 DE DE19853529203 patent/DE3529203A1/de not_active Ceased
- 1985-08-21 GB GB08520929A patent/GB2163453A/en not_active Withdrawn
- 1985-08-24 JP JP18652385A patent/JPS61108371A/ja active Granted
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4943535A (en) * | 1985-06-20 | 1990-07-24 | Celltech Limited | Anti-lift fermenter |
| WO2014110512A1 (en) * | 2013-01-11 | 2014-07-17 | Pbs Biotech, Inc. | Method and apparatus for the use of micro-carriers in a disposable bioreactor system |
| US9637717B2 (en) | 2013-01-11 | 2017-05-02 | Pbs Biotech, Inc. | Method and apparatus for the use of micro-carriers in a disposable bioreactor system |
| US10059915B2 (en) | 2013-01-11 | 2018-08-28 | Pbs Biotech, Inc. | Cell culture method in a bioreactor system using micro-carriers |
| WO2019002608A1 (fr) * | 2017-06-30 | 2019-01-03 | Universite Paris Diderot Paris 7 | Systeme fluidique de production de vesicules extracellulaires et procede associe |
| FR3068361A1 (fr) * | 2017-06-30 | 2019-01-04 | Universite Paris Diderot Paris 7 | Systeme fluidique de production de vesicules extracellulaires et procede associe |
| US20200385665A1 (en) * | 2017-06-30 | 2020-12-10 | Universite Paris Diderot Paris 7 | Fluid system for producing extracellular vesicles and associated method |
| AU2018294559B2 (en) * | 2017-06-30 | 2023-09-28 | Centre National De La Recherche Scientifique (Cnrs) | Fluid system for producing extracellular vesicles and associated method |
| US12098355B2 (en) * | 2017-06-30 | 2024-09-24 | Universite De Paris | Fluid system for producing extracellular vesicles and related method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61108371A (ja) | 1986-05-27 |
| DE3529203A1 (de) | 1986-02-27 |
| GB8520929D0 (en) | 1985-09-25 |
| JPS6260074B2 (enrdf_load_stackoverflow) | 1987-12-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |