GB1411422A - Methods for purifying urokinase preparations - Google Patents
Methods for purifying urokinase preparationsInfo
- Publication number
- GB1411422A GB1411422A GB1550275A GB1550275A GB1411422A GB 1411422 A GB1411422 A GB 1411422A GB 1550275 A GB1550275 A GB 1550275A GB 1550275 A GB1550275 A GB 1550275A GB 1411422 A GB1411422 A GB 1411422A
- Authority
- GB
- United Kingdom
- Prior art keywords
- urokinase
- solution
- conductivity
- micromhos
- ammonium sulphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 title abstract 13
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title abstract 13
- 229960005356 urokinase Drugs 0.000 title abstract 13
- 238000000034 method Methods 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title 1
- 239000000243 solution Substances 0.000 abstract 14
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 5
- 239000001166 ammonium sulphate Substances 0.000 abstract 5
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 5
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 239000002244 precipitate Substances 0.000 abstract 3
- 239000011347 resin Substances 0.000 abstract 3
- 229920005989 resin Polymers 0.000 abstract 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 abstract 2
- 238000005194 fractionation Methods 0.000 abstract 2
- 210000002700 urine Anatomy 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 abstract 1
- 239000005995 Aluminium silicate Substances 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 abstract 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 abstract 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000002671 adjuvant Substances 0.000 abstract 1
- 235000012211 aluminium silicate Nutrition 0.000 abstract 1
- 229910021529 ammonia Inorganic materials 0.000 abstract 1
- 235000019270 ammonium chloride Nutrition 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000001768 carboxy methyl cellulose Substances 0.000 abstract 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 abstract 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 230000001698 pyrogenic effect Effects 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 238000005185 salting out Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7134435A FR2153200A1 (en) | 1971-09-24 | 1971-09-24 | Stable,pyrogen-free urokinase prepns - from human urine |
| FR7223868A FR2190413A2 (en) | 1972-06-30 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
| FR7223867A FR2190412A1 (en) | 1972-06-30 | 1972-06-30 | Stable,pyrogen-free urokinase prepns - from human urine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1411422A true GB1411422A (en) | 1975-10-22 |
Family
ID=27249660
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4400372A Expired GB1411421A (en) | 1971-09-24 | 1972-09-22 | Urokinaseheparin complex |
| GB1550275A Expired GB1411422A (en) | 1971-09-24 | 1972-09-22 | Methods for purifying urokinase preparations |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4400372A Expired GB1411421A (en) | 1971-09-24 | 1972-09-22 | Urokinaseheparin complex |
Country Status (11)
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2428248B2 (de) * | 1973-07-24 | 1978-01-12 | Serna Ag, Glarus (Schweiz) | Verfahren zur herstellung von pyrogenfreier urokinase |
| US3957582A (en) * | 1974-11-20 | 1976-05-18 | Abbott Laboratories | Purification of urokinase |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3256158A (en) * | 1963-03-22 | 1966-06-14 | Abbott Lab | Purification of urokinase |
| US3355361A (en) * | 1964-10-15 | 1967-11-28 | Sterling Drug Inc | Recovery and purification of urokinase |
| US3477912A (en) * | 1967-07-07 | 1969-11-11 | Century Lab Inc | Method of production of urokinase |
| US3477913A (en) * | 1967-10-31 | 1969-11-11 | Century Lab Inc | Method of production of urokinase |
-
0
- BE BE789189D patent/BE789189A/xx not_active IP Right Cessation
-
1972
- 1972-09-21 SE SE7212220A patent/SE405806B/sv unknown
- 1972-09-22 CA CA152,328A patent/CA986013A/en not_active Expired
- 1972-09-22 CH CH1715974A patent/CH604726A5/xx not_active IP Right Cessation
- 1972-09-22 GB GB4400372A patent/GB1411421A/en not_active Expired
- 1972-09-22 NL NL7212891A patent/NL7212891A/xx active Search and Examination
- 1972-09-22 CH CH1384072A patent/CH560050A5/xx not_active IP Right Cessation
- 1972-09-22 JP JP9561172A patent/JPS5718465B2/ja not_active Expired
- 1972-09-22 DK DK470672A patent/DK133130C/da not_active IP Right Cessation
- 1972-09-22 GB GB1550275A patent/GB1411422A/en not_active Expired
- 1972-09-23 ES ES406980A patent/ES406980A1/es not_active Expired
- 1972-09-25 DE DE2246969A patent/DE2246969C2/de not_active Expired
-
1973
- 1973-09-17 IN IN2122/CAL/1973A patent/IN141112B/en unknown
-
1975
- 1975-08-14 ES ES440244A patent/ES440244A1/es not_active Expired
- 1975-12-30 SE SE7514792A patent/SE7514792L/xx unknown
-
1977
- 1977-02-16 ES ES455975A patent/ES455975A1/es not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE2246969C2 (de) | 1986-12-11 |
| GB1411421A (en) | 1975-10-22 |
| ES406980A1 (es) | 1976-06-16 |
| SE405806B (sv) | 1979-01-08 |
| SE7514792L (sv) | 1975-12-30 |
| JPS4856888A (enrdf_load_stackoverflow) | 1973-08-09 |
| CA986013A (en) | 1976-03-23 |
| CH604726A5 (enrdf_load_stackoverflow) | 1978-09-15 |
| ES455975A1 (es) | 1978-03-01 |
| DK133130B (da) | 1976-03-29 |
| CH560050A5 (enrdf_load_stackoverflow) | 1975-03-27 |
| ES440244A1 (es) | 1977-08-01 |
| JPS5718465B2 (enrdf_load_stackoverflow) | 1982-04-16 |
| NL7212891A (enrdf_load_stackoverflow) | 1973-03-27 |
| DK133130C (da) | 1976-09-27 |
| IN141112B (enrdf_load_stackoverflow) | 1977-01-22 |
| BE789189A (fr) | 1973-03-22 |
| DE2246969A1 (de) | 1973-04-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PCNP | Patent ceased through non-payment of renewal fee |