GB1191697A - Laboratory Reagent for Assay of Lactate Dehydrogenase - Google Patents

Laboratory Reagent for Assay of Lactate Dehydrogenase

Info

Publication number
GB1191697A
GB1191697A GB29934/67A GB2993467A GB1191697A GB 1191697 A GB1191697 A GB 1191697A GB 29934/67 A GB29934/67 A GB 29934/67A GB 2993467 A GB2993467 A GB 2993467A GB 1191697 A GB1191697 A GB 1191697A
Authority
GB
United Kingdom
Prior art keywords
dry
lactate dehydrogenase
dpn
lactate
june
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB29934/67A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Chemicals Inc
Original Assignee
Calbiochem Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calbiochem Corp filed Critical Calbiochem Corp
Publication of GB1191697A publication Critical patent/GB1191697A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1,191,697. Determination of lactate dehydrogenase. CALBIOCHEM. 28 June, 1967 [30 June, 1966], No. 29934/67. Heading B1X. A solid assay material for dissolving in water for determination of lactate dehydrogenase (LDH) comprises the dry substrate lithium lactate, the dry coenzyme diphosphopyridine nucleotide (DPN), at least one dry stabilizing/bulking agent selected from sorbitol, lactose and mannitol, and a dry buffer capable of maintaining the pH at from 8.0 to 9.0, and preferably 8.4 to 8.6. The buffer and bulking agent are ground together, the substrate and the DPN are mixed and added, and the resulting powder is ground and packaged in unit amounts. In use, the contents of one package are dissolved in water and the solution mixed with the sample, for example a serum under test. The rate of change of optical density of the solution at 340mu is recorded as a measure of the lactate dehydrogenase content of the sample. The reaction concerned is LDH lactate+DPN # pyruvate+DPNH.
GB29934/67A 1966-06-30 1967-06-28 Laboratory Reagent for Assay of Lactate Dehydrogenase Expired GB1191697A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US561757A US3413198A (en) 1966-06-30 1966-06-30 Reagents and method for assaying biological samples

Publications (1)

Publication Number Publication Date
GB1191697A true GB1191697A (en) 1970-05-13

Family

ID=24243318

Family Applications (3)

Application Number Title Priority Date Filing Date
GB29939/67A Expired GB1192046A (en) 1966-06-30 1967-06-28 Laboratory Reagent for Assay of Urea
GB29940/67A Expired GB1163409A (en) 1966-06-30 1967-06-28 Laboratory Reagent for Assay of Creatine Phosphokinase
GB29934/67A Expired GB1191697A (en) 1966-06-30 1967-06-28 Laboratory Reagent for Assay of Lactate Dehydrogenase

Family Applications Before (2)

Application Number Title Priority Date Filing Date
GB29939/67A Expired GB1192046A (en) 1966-06-30 1967-06-28 Laboratory Reagent for Assay of Urea
GB29940/67A Expired GB1163409A (en) 1966-06-30 1967-06-28 Laboratory Reagent for Assay of Creatine Phosphokinase

Country Status (7)

Country Link
US (1) US3413198A (en)
BE (1) BE700778A (en)
CH (1) CH514841A (en)
DE (3) DE1598321A1 (en)
GB (3) GB1192046A (en)
IL (1) IL28098A (en)
NL (1) NL6708570A (en)

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US3523871A (en) * 1966-07-20 1970-08-11 Eisai Co Ltd Stabilization of catalase
DE2050267C3 (en) * 1970-10-13 1974-06-12 Boehringer Mannheim Gmbh, 6800 Mannheim Process for the preparation of stable preparations of reduced nicotinamide adenine dinucleotide
BE786291A (en) * 1971-07-20 1973-01-15 Technicon Instr DIAGNOSTIC COMPOSITIONS FOR DETERMINATION OF GLUTAMATE-OXALATE-TRANSAMINASE (GOT) AND GLUTAMATE-PYRUVATE-TRANSAMINASE (GPT)
US3926735A (en) * 1971-10-20 1975-12-16 Mallinckrodt Inc Alkaline phosphatase assay
US3953295A (en) * 1971-10-20 1976-04-27 Mallinckrodt, Inc. Reagent formulations for glucose assay
US3928137A (en) * 1971-10-20 1975-12-23 Mallinckrodt Inc Reagent formulation for uric acid assay
CS164231B2 (en) * 1972-09-28 1975-11-07
US3869348A (en) * 1973-02-26 1975-03-04 Pierce Chemical Co Determination of amylase
US3953297A (en) * 1973-02-26 1976-04-27 Pierce Chemical Company Determination of amylase
US3941659A (en) * 1974-07-11 1976-03-02 Honeywell Inc. Blood alcohol analyzer
US3982999A (en) * 1974-07-26 1976-09-28 Kharasch Jerome A Complexing cresolase with copper chelating agents
US3926736A (en) * 1974-08-30 1975-12-16 Calbiochem Enzymatic ethanol assay
US4067773A (en) * 1975-09-02 1978-01-10 William Zinsser & Co. Enzyme-containing article for removing paper adhered to a surface
US4006061A (en) * 1975-12-29 1977-02-01 Monsanto Company Lactate dehydrogenase determination method
CH625626A5 (en) * 1976-03-25 1981-09-30 Hoffmann La Roche Process for the preparation of stable immunological reagents
US4105800A (en) * 1976-07-26 1978-08-08 Board Of Regents For Education Of The State Of Rhode Island Immobilized enzyme method to assess fish quality
US4080263A (en) * 1976-08-11 1978-03-21 Boehringer Mannheim Gmbh Process and reagent for the rapid quantitative determination of lactate or alanine
NL7610608A (en) * 1976-09-24 1978-03-29 Akzo Nv PROCESS FOR STABILIZING PEROXIDASE-CONTAINING COMPOSITIONS.
US4127502A (en) * 1977-06-10 1978-11-28 Eastman Kodak Company Stabilizers for reconstituted, lyophilized samples
SE7905852L (en) * 1979-07-04 1981-01-05 Lkb Produkter Ab PROCEDURE FOR DETERMINING CREATINKINAS
SE432112B (en) * 1979-07-12 1984-03-19 Lkb Produkter Ab PROCEDURE FOR DETERMINING CREATINKINASES IN SAMPLES CONTAINING ATP
US4378430A (en) * 1979-09-11 1983-03-29 Modrovich Ivan Endre Method of forming stabilized urease solutions
US4282316A (en) * 1979-09-11 1981-08-04 Modrovich Ivan Endre Stabilized enzymic solutions for determining urea
US4465770A (en) * 1979-09-11 1984-08-14 Modrovich Ivan Endre Stabilized enzymic solutions for determining urea
US4394449A (en) * 1980-02-13 1983-07-19 Modrovich Ivan Endre Stabilization of coenzymes in aqueous solution
US4321364A (en) * 1980-04-17 1982-03-23 Minister For Public Works For The State Of New South Wales Preparation of soluble chromogenic substrates
JPS56169598A (en) * 1980-05-26 1981-12-26 Mitsubishi Petrochem Co Ltd Measuring composition
CA1164340A (en) * 1980-09-02 1984-03-27 Alex A. Monte Single test compositions for immunoassays
DE3044454C2 (en) * 1980-11-26 1982-12-09 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilized enzyme preparation
US4366243A (en) * 1981-04-17 1982-12-28 Miles Laboratories, Inc. Stabilization of glucose oxidase apoenzyme
US4464469A (en) * 1981-09-01 1984-08-07 John & E. Sturge Limited Stabilized lactase solutions and processes for stabilization
JPS58209993A (en) * 1982-05-31 1983-12-07 Mochida Pharmaceut Co Ltd Production of interferon
DE3303098A1 (en) * 1983-01-31 1984-08-02 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD AND REAGENT FOR DETERMINING GLUCOSE IN HAEMOLYSED BLOOD
US4663295A (en) * 1983-06-29 1987-05-05 Ciba Corning Diagnostics Corp. Estrogen-progesterone control reagents and methods for making same
US4900666A (en) * 1983-07-12 1990-02-13 Lifescan, Inc. Colorimetric ethanol analysis method and test device
US4734360A (en) * 1983-07-12 1988-03-29 Lifescan, Inc. Colorimetric ethanol analysis method and test device
US4810633A (en) * 1984-06-04 1989-03-07 Miles Inc. Enzymatic ethanol test
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US4990445A (en) * 1988-01-20 1991-02-05 Beckman Instruments, Inc. Stable reagent and kinetic assay for alpha-amylase
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DE4103220A1 (en) * 1991-02-02 1992-08-06 Boehringer Mannheim Gmbh METHOD FOR STABILIZING 1-METHYLHYDANTOINASE, USE OF A STABILIZED 1-METHYLHYDANTOINASE FOR DETERMINING AN ANALYTIC, CORRESPONDING DETERMINATION PROCEDURE, AND APPROPRIATE AGENTS
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DE683524C (en) * 1934-07-08 1939-11-09 Max Winckel Dr Process for transferring solutions of chemically labile substances in dry form
US3072532A (en) * 1958-11-04 1963-01-08 Innerfield Irving Administration of enzymic composition
US2990338A (en) * 1959-11-24 1961-06-27 Gibson Jacob John Composition for determination of glucose in body fluids
US3133001A (en) * 1959-11-26 1964-05-12 Muset Pedro Puig Stabilization of enzymes

Also Published As

Publication number Publication date
CH514841A (en) 1971-10-31
NL6708570A (en) 1968-01-02
GB1192046A (en) 1970-05-13
DE1598326A1 (en) 1972-03-02
DE1598325B1 (en) 1973-01-11
DE1598321A1 (en) 1972-02-24
IL28098A (en) 1976-02-29
BE700778A (en) 1968-01-02
GB1163409A (en) 1969-09-04
US3413198A (en) 1968-11-26

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PLNP Patent lapsed through nonpayment of renewal fees