GB1176968A - Composition for the Determination of Enzymes - Google Patents

Composition for the Determination of Enzymes

Info

Publication number
GB1176968A
GB1176968A GB4119767A GB4119767A GB1176968A GB 1176968 A GB1176968 A GB 1176968A GB 4119767 A GB4119767 A GB 4119767A GB 4119767 A GB4119767 A GB 4119767A GB 1176968 A GB1176968 A GB 1176968A
Authority
GB
United Kingdom
Prior art keywords
alanine
solution
colour reagent
naphthylamide
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4119767A
Inventor
Reinhard Haschen
Wolfgang Farr
Dieter Reichelt
Norbert Rehfeld
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AWD Pharma GmbH and Co KG
Original Assignee
Arzneimittelwerk Dresden GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arzneimittelwerk Dresden GmbH filed Critical Arzneimittelwerk Dresden GmbH
Priority to GB4119767A priority Critical patent/GB1176968A/en
Priority to FR123451A priority patent/FR1540617A/en
Priority to ES358216A priority patent/ES358216A1/en
Publication of GB1176968A publication Critical patent/GB1176968A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1,176,968. Determining amino acid arylamidase. ARZNEIMITTELWERK DRESDEN VEB. 8 Sept., 1967, No. 41197/67. Heading B1X. A composition for the determination of amino acid arylamidase (leucinaminopeptidase) comprises (a) alanine hydrazide, alanine pnitroanilide or alanine-#-naphthylamide, (b) a buffer solution with a pH of 6.5 to 7.5, with or without (c) an aromatic aldehyde colour reagent, for example p-dimethylamino-benzaldehyde. In use, the sample, for example blood serum, is incubated with the substrate and the buffer solution. When alanine p-nitroanilide is used, the p-nitro-aniline formed is determined colorimetrically, optionally in a kinetic test. When alanine hydrazide or alanine-#-naphthylamide is used, the hydrazine or #-naphthylamine formed react with the aromatic aldehyde colour reagent and the solution is acidified to give a coloured salt, the extinction of which is measured. A test pack contains solutions of the alanine derivative and of the buffer, optionally mixed together, with or without a solution of the colour reagent.
GB4119767A 1967-09-08 1967-09-08 Composition for the Determination of Enzymes Expired GB1176968A (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GB4119767A GB1176968A (en) 1967-09-08 1967-09-08 Composition for the Determination of Enzymes
FR123451A FR1540617A (en) 1967-09-08 1967-10-05 Enzyme assay method
ES358216A ES358216A1 (en) 1967-09-08 1968-09-06 Composition for the Determination of Enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4119767A GB1176968A (en) 1967-09-08 1967-09-08 Composition for the Determination of Enzymes

Publications (1)

Publication Number Publication Date
GB1176968A true GB1176968A (en) 1970-01-07

Family

ID=10418565

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4119767A Expired GB1176968A (en) 1967-09-08 1967-09-08 Composition for the Determination of Enzymes

Country Status (2)

Country Link
ES (1) ES358216A1 (en)
GB (1) GB1176968A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0152274A2 (en) * 1984-02-07 1985-08-21 Kyowa Medex Co. Ltd. Method for the determination of Leucine Aminopeptidase (LAP)
EP0165905A1 (en) * 1984-06-18 1985-12-27 The Board Of Regents Of The University Of Michigan Diagnostic assay method for the detection of oral pathogenic bacteria mixtures

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0152274A2 (en) * 1984-02-07 1985-08-21 Kyowa Medex Co. Ltd. Method for the determination of Leucine Aminopeptidase (LAP)
EP0152274A3 (en) * 1984-02-07 1989-10-11 Kyowa Medex Co. Ltd. Method for the determination of enzyme activity
EP0165905A1 (en) * 1984-06-18 1985-12-27 The Board Of Regents Of The University Of Michigan Diagnostic assay method for the detection of oral pathogenic bacteria mixtures

Also Published As

Publication number Publication date
ES358216A1 (en) 1971-02-16

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