IL28098A - Stable reagent mixtures containing polyhydric compounds - Google Patents
Stable reagent mixtures containing polyhydric compoundsInfo
- Publication number
- IL28098A IL28098A IL28098A IL2809867A IL28098A IL 28098 A IL28098 A IL 28098A IL 28098 A IL28098 A IL 28098A IL 2809867 A IL2809867 A IL 2809867A IL 28098 A IL28098 A IL 28098A
- Authority
- IL
- Israel
- Prior art keywords
- dry
- specimen
- reagent
- enzyme
- substance
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims 25
- 239000000203 mixture Substances 0.000 title claims 9
- 150000001875 compounds Chemical class 0.000 title 1
- 238000003556 assay Methods 0.000 claims 12
- 239000007788 liquid Substances 0.000 claims 12
- 239000000126 substance Substances 0.000 claims 12
- 239000007787 solid Substances 0.000 claims 10
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims 8
- 102000004190 Enzymes Human genes 0.000 claims 8
- 108090000790 Enzymes Proteins 0.000 claims 8
- 229930195725 Mannitol Natural products 0.000 claims 8
- 239000005515 coenzyme Substances 0.000 claims 8
- 239000000594 mannitol Substances 0.000 claims 8
- 235000010355 mannitol Nutrition 0.000 claims 8
- 230000003287 optical effect Effects 0.000 claims 8
- 239000000872 buffer Substances 0.000 claims 7
- 239000000463 material Substances 0.000 claims 7
- 239000003381 stabilizer Substances 0.000 claims 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 7
- 239000004067 bulking agent Substances 0.000 claims 6
- 238000000034 method Methods 0.000 claims 6
- 239000000758 substrate Substances 0.000 claims 6
- 102000013460 Malate Dehydrogenase Human genes 0.000 claims 5
- 108010026217 Malate Dehydrogenase Proteins 0.000 claims 5
- 239000000243 solution Substances 0.000 claims 5
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims 4
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims 4
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 claims 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 3
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 3
- 229910019142 PO4 Inorganic materials 0.000 claims 3
- 229960001484 edetic acid Drugs 0.000 claims 3
- 239000008103 glucose Substances 0.000 claims 3
- 239000008101 lactose Substances 0.000 claims 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims 3
- 235000021317 phosphate Nutrition 0.000 claims 3
- 239000000600 sorbitol Substances 0.000 claims 3
- KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims 2
- 102100031126 6-phosphogluconolactonase Human genes 0.000 claims 2
- 108010029731 6-phosphogluconolactonase Proteins 0.000 claims 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims 2
- 241000220479 Acacia Species 0.000 claims 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims 2
- 108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 claims 2
- 102000005548 Hexokinase Human genes 0.000 claims 2
- 108700040460 Hexokinases Proteins 0.000 claims 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims 2
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims 2
- HDVCHBLHEICPPP-UHFFFAOYSA-N O=P(=O)C1=CC=NC(P(=O)=O)=C1P(=O)=O Chemical class O=P(=O)C1=CC=NC(P(=O)=O)=C1P(=O)=O HDVCHBLHEICPPP-UHFFFAOYSA-N 0.000 claims 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 2
- 102000003929 Transaminases Human genes 0.000 claims 2
- 108090000340 Transaminases Proteins 0.000 claims 2
- 229910052783 alkali metal Inorganic materials 0.000 claims 2
- -1 alkali metal salts Chemical class 0.000 claims 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims 2
- 235000003704 aspartic acid Nutrition 0.000 claims 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims 2
- 230000002255 enzymatic effect Effects 0.000 claims 2
- 229930195712 glutamate Natural products 0.000 claims 2
- GKQWYZBANWAFMQ-UHFFFAOYSA-M lithium;2-hydroxypropanoate Chemical compound [Li+].CC(O)C([O-])=O GKQWYZBANWAFMQ-UHFFFAOYSA-M 0.000 claims 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 229940054269 sodium pyruvate Drugs 0.000 claims 2
- 230000000087 stabilizing effect Effects 0.000 claims 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims 1
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- HWXBTNAVRSUOJR-UHFFFAOYSA-N alpha-hydroxyglutaric acid Natural products OC(=O)C(O)CCC(O)=O HWXBTNAVRSUOJR-UHFFFAOYSA-N 0.000 claims 1
- 229940009533 alpha-ketoglutaric acid Drugs 0.000 claims 1
- 239000013060 biological fluid Substances 0.000 claims 1
- 239000000470 constituent Substances 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 claims 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims 1
- 235000019796 monopotassium phosphate Nutrition 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical class OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Claims (5)
1. A dry stable reagent mixtur for assaying biological fluids* characterized by comprising at least one moisture sensitive, biologically active, enzymatic constituent, combined with a bulking-stabllizlng agent which comprises a polyhydric substance selected from the group consisting of mannitol, sorbitol and lactose*
2. Method of stabilizing a moisture sensitive enzymatic material which comprises! talcing said material while in a dry particulate state and admixing therewith a polyhydric substance selected from mannitol, sorbitol and lactose as stabilizing bulking agents, said su stances ranging for each part by weight of said material from 5 parts to 2000 parts by weight,
3. The method of claim 2, wherein the substance is mannitol.
4. A solid assay material according to claim l, for dissolving in water to create a liquid reagent for assaying a specimen for glutamate ©xaloacetate transaminase,including the combination oft substrates comprising dry aspartic acid and alpha-keto-glutaric acidI the dry enzyme malate dehydrogenases the dry coenzyme reduced diphosphopyridine nucleotidei at least one dry stabilizer from the class including mucilageneous gums, polysaccharides,hydroxyalkylaraines,ethylene diamine tetraacetic acid and its salts, and a sulfate anient a dry buffer from the class that includes the alkali metal salts and phosphates! the dry atabilizing-bulklng agent mannitoli and each substance above being present in that quantity so as to ensure a uniform rate of the reaction catalyzed by the enzyme being determined and so as to aauee said rate to be optimal or maximal.
5. The method of assaying a biological specimen for the enzyme glutamate oxaloacetate transaminase, including the steps of: dissolving in water, a substantially anhydrous solid reagent comprising: the dry substrates aspartic acid and alpha-keto-glutaric acid; the dry enzyme malate dehydrogenase; the dry coenzyme reduced diphosphopyridine nucleotide; at least one dry stabilizer from the class that includes mucilagenous gums, hydroxyalkylamines, ethylene diamine tetracetic acid and its salts, and a sulfate anion; a dry buffer from the class that includes the alkali metal salts of phosphates; the dry stabilizing-bulking agent mannitol; each substance being present in that quantity to insure a uniform rate of reaction catalyzed by the enzyme being determined, thereby to produce a liquid reagent having a measurable optical density; mixing said liquid reagent with said specimen to form a specimen reagent assay mixture; determining the rate of change of the optical density of the reacting specimen-reagent assay mixture; and obtaining a solution containing the enzyme malate dehydrogenase in aqueous ammonium sulfate; dissolving into said solution at least one stabilizer from the class of tris (hydroxymethyl)aminomethane and its sulfate salt, acacia and ethylenediamine tetraacetic acid; mixing the solution until the malate dehydrogenase and the stabilizer are uniformly distributed throughout the resultant solution; freezing the resultant solution to form a solid mass; and retaining the frozen mass under a vacuum until the moisture in the mass has been removed and the enzyme malate dehydrogenase and the stabilizers are in a dry solid form. accordipg to claim 1» 6· A solid assay materiay for dissolving in water to create a liquid reagent for assaying a specimen for lactate dehydrogenase, including the combination of? the dry substrate sodium pyruvate; the dry coenzyme reduced diphosphopyridine nucleotide; a dry buffer from the class that includes disodium hydrogen phosphate and potassium dehydrogen phosphate; the dry stabilizing-bulking agent mannitol; and each substance above being present in that quantity so as to insure a uniform rate of reaction catalyzed by the enzyme being determined and so as to cause said rate to be optimal or maximal= 7 The method of assaying a specimen for the enzyme lactate dehydrogenase, including the steps of dissolving into water, a substantially anhydrous solid reagent material, comprising^ the dry substrate sodium pyruvate; the dry coenzyme reduced diphosphopyridine nucleotide; a dry buffer comprising disodium hydrogen phosphate and potassium dihydrogen phosphate; the dry bulking-stabilizing agent mannitol; each substance being present in that quantity to insure a uniform rate of reaction catalyzed by the enzyme being determined, thereby to produce a liquid reagent having a measurable optical density; mixing said liquid reagent with said specimen to form a specimen-reagent assay mixture, and measuring the rate of change optical density of the reacting specimen-assay mixture. Λ . , . according to c.laim 1 S* A solid assay material/ for dissolving in water to create a liquid reagent for assaying a specimen for glucose, including the combination of; the dry enzymes hexokinase and glucose-6-phosphate dehydrogenase; the dry coenzymes adenosine triphosphate and triphosphopyridine nucleotide; at least one dry stabilizer selected from acacia, tris-sulfate and ammonium sulfate the dry buffer tris°succinate; the dry stabilizing-bulking agent mannitol; and each substance above being present in that quantity so as to insure a uniform rate of reaction catalyzed by the enzyme being determined and so as to cause said rate to be optimal or maximal.. . The method of assaying a specimen for glucose including the steps of dissolving into water a substantially anhydrous solid reagent comprising? the dry enzymes hexokinase and glucose-6-phosphate dehydrogenase; the dry coenzymes adenosine triphosphate and triphosphopyridine nucleotide; and the dry stabilizing-bulkifig i¾ent aannitol; each substance being present in that quantity so as to insure that the amount of reduced triphosphopyridine nucleotide formed is equivalent to the amount of glucose present, thereby to produce a liquid reagent having a measurable optical density; mixing said liquid reagent, with said specimen to form a specimen-reagent assay mixture; and determining the amount of change in the optical density of the reacted specimen reagent assay mixture. according to claim 1 40» A solid assay material/for dissolving in water to provide a liquid reagent for assaying a specimen for the unknown lactate dehydrogenase, including the combination of: the dry substrate comprising lithium lactate; the dry coenzyme comprising diphosphopyridine nucleotide; at least one dry stabilizing-bulking agent selected from the class that includes polyhydric substances; a dry buffer capable of maintaining the pH between each substance above being present in that quantity so as to insure a uniform rate of reaction catalyzed by the enzyme being determined, and so as to cause said rate to be optimal or maximal. 44» The method of assaying a specimen for the enzyme lactate dehydrogenase including the steps of dissolving a solid reagent comprising; a dry substrate lithium lactate; the dry coenzyme diphcsphopyridine nucleotide; at least one dry stabilizing-bulking agent selected from mannitol, sorbitol and lactose; a dry buffer capable of maintaining the pH between each substance being present in that quantity so as to insure a uniform rate of reaction catalyzed by the enzyme being determined, thereby to produce a liquid reagent having a measurable optical density^ mixing said liquid reagent with said specimen to form said specimen -reagent assay mixture and measuring the rate of change in optical density of the reacting specimen=reagent assay mixture,, For
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US561757A US3413198A (en) | 1966-06-30 | 1966-06-30 | Reagents and method for assaying biological samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL28098A true IL28098A (en) | 1976-02-29 |
Family
ID=24243318
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL28098A IL28098A (en) | 1966-06-30 | 1967-06-01 | Stable reagent mixtures containing polyhydric compounds |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3413198A (en) |
| BE (1) | BE700778A (en) |
| CH (1) | CH514841A (en) |
| DE (3) | DE1598325B1 (en) |
| GB (3) | GB1191697A (en) |
| IL (1) | IL28098A (en) |
| NL (1) | NL6708570A (en) |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3523871A (en) * | 1966-07-20 | 1970-08-11 | Eisai Co Ltd | Stabilization of catalase |
| DE2050267C3 (en) * | 1970-10-13 | 1974-06-12 | Boehringer Mannheim Gmbh, 6800 Mannheim | Process for the preparation of stable preparations of reduced nicotinamide adenine dinucleotide |
| BE786291A (en) * | 1971-07-20 | 1973-01-15 | Technicon Instr | DIAGNOSTIC COMPOSITIONS FOR DETERMINATION OF GLUTAMATE-OXALATE-TRANSAMINASE (GOT) AND GLUTAMATE-PYRUVATE-TRANSAMINASE (GPT) |
| US3928137A (en) * | 1971-10-20 | 1975-12-23 | Mallinckrodt Inc | Reagent formulation for uric acid assay |
| US3926735A (en) * | 1971-10-20 | 1975-12-16 | Mallinckrodt Inc | Alkaline phosphatase assay |
| US3953295A (en) * | 1971-10-20 | 1976-04-27 | Mallinckrodt, Inc. | Reagent formulations for glucose assay |
| CS164231B2 (en) * | 1972-09-28 | 1975-11-07 | ||
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Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE683524C (en) * | 1934-07-08 | 1939-11-09 | Max Winckel Dr | Process for transferring solutions of chemically labile substances in dry form |
| US3072532A (en) * | 1958-11-04 | 1963-01-08 | Innerfield Irving | Administration of enzymic composition |
| US2990338A (en) * | 1959-11-24 | 1961-06-27 | Gibson Jacob John | Composition for determination of glucose in body fluids |
| US3133001A (en) * | 1959-11-26 | 1964-05-12 | Muset Pedro Puig | Stabilization of enzymes |
-
1966
- 1966-06-30 US US561757A patent/US3413198A/en not_active Expired - Lifetime
-
1967
- 1967-06-01 IL IL28098A patent/IL28098A/en unknown
- 1967-06-20 NL NL6708570A patent/NL6708570A/xx unknown
- 1967-06-28 GB GB29934/67A patent/GB1191697A/en not_active Expired
- 1967-06-28 GB GB29939/67A patent/GB1192046A/en not_active Expired
- 1967-06-28 GB GB29940/67A patent/GB1163409A/en not_active Expired
- 1967-06-28 CH CH913967A patent/CH514841A/en not_active IP Right Cessation
- 1967-06-30 DE DE1598325A patent/DE1598325B1/en active Granted
- 1967-06-30 BE BE700778D patent/BE700778A/xx not_active IP Right Cessation
- 1967-06-30 DE DE19671598326 patent/DE1598326A1/en active Pending
- 1967-06-30 DE DE19671598321 patent/DE1598321A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| GB1191697A (en) | 1970-05-13 |
| DE1598326A1 (en) | 1972-03-02 |
| DE1598321A1 (en) | 1972-02-24 |
| NL6708570A (en) | 1968-01-02 |
| CH514841A (en) | 1971-10-31 |
| BE700778A (en) | 1968-01-02 |
| GB1192046A (en) | 1970-05-13 |
| DE1598325B1 (en) | 1973-01-11 |
| US3413198A (en) | 1968-11-26 |
| GB1163409A (en) | 1969-09-04 |
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