FR3096146B1 - Dispositif de creation d’un champ evanescent a motifs sur une surface et procede associe - Google Patents

Dispositif de creation d’un champ evanescent a motifs sur une surface et procede associe Download PDF

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Publication number
FR3096146B1
FR3096146B1 FR1905159A FR1905159A FR3096146B1 FR 3096146 B1 FR3096146 B1 FR 3096146B1 FR 1905159 A FR1905159 A FR 1905159A FR 1905159 A FR1905159 A FR 1905159A FR 3096146 B1 FR3096146 B1 FR 3096146B1
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FR
France
Prior art keywords
diopter
patterns
light
creating
injection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1905159A
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English (en)
Other versions
FR3096146A1 (fr
Inventor
Alexeï Grichine
Olivier Destaing
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite Grenoble Alpes
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite Grenoble Alpes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite Grenoble Alpes filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR1905159A priority Critical patent/FR3096146B1/fr
Priority to US17/611,421 priority patent/US20220214556A1/en
Priority to JP2021568495A priority patent/JP2022532248A/ja
Priority to PCT/IB2020/054529 priority patent/WO2020230051A1/fr
Priority to EP20731212.5A priority patent/EP3969954A1/fr
Publication of FR3096146A1 publication Critical patent/FR3096146A1/fr
Application granted granted Critical
Publication of FR3096146B1 publication Critical patent/FR3096146B1/fr
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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/56—Optics using evanescent waves, i.e. inhomogeneous waves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Optics & Photonics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Lenses (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Dispositif de création d’un champ évanescent à motifs sur une surface et procédé associé L’invention concerne un dispositif (1) de création d’un champ évanescent à motifs sur la surface (S) d’un dioptre comprenant un objectif (O), dans un plan focal image duquel est disposée la surface (S) du dioptre, un élément (2) d’injection de lumière émettant un faisceau de lumière (B) collimaté de diamètre, un montage optique (4) entre l’élément (2) d’injection de lumière et l’objectif (O) par lequel le plan objet de l’objectif (O) est conjugué optiquement avec le plan image de l’élément (2) d’injection de lumière, le montage optique (4) étant configuré pour qu’un faisceau de lumière collimaté (B) provenant de l’élément (4) d’injection de lumière soit émis vers l’objectif (O) pour être renvoyé vers la surface (S) du dioptre avec un angle d’incidence supérieur ou égal à l’angle critique du dioptre, un dispositif optique (FS1) de formation de motifs dans le plan objet de l’élément (2), de telle sorte que le motif formé par le dispositif optique de formation de motifs en lumière transmise sur le faisceau de lumière dans le plan objet de l’élément (2) se retrouve en surface du dioptre.
FR1905159A 2019-05-16 2019-05-16 Dispositif de creation d’un champ evanescent a motifs sur une surface et procede associe Active FR3096146B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR1905159A FR3096146B1 (fr) 2019-05-16 2019-05-16 Dispositif de creation d’un champ evanescent a motifs sur une surface et procede associe
US17/611,421 US20220214556A1 (en) 2019-05-16 2020-05-13 Device for creating a patterned evanescent field on a surface and method therefor
JP2021568495A JP2022532248A (ja) 2019-05-16 2020-05-13 表面にパターン化されたエバネッセント場を形成する装置およびその方法
PCT/IB2020/054529 WO2020230051A1 (fr) 2019-05-16 2020-05-13 Dispositif de creation d'un champ evanescent a motifs sur une surface et procede associe
EP20731212.5A EP3969954A1 (fr) 2019-05-16 2020-05-13 Dispositif de creation d'un champ evanescent a motifs sur une surface et procede associe

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1905159 2019-05-16
FR1905159A FR3096146B1 (fr) 2019-05-16 2019-05-16 Dispositif de creation d’un champ evanescent a motifs sur une surface et procede associe

Publications (2)

Publication Number Publication Date
FR3096146A1 FR3096146A1 (fr) 2020-11-20
FR3096146B1 true FR3096146B1 (fr) 2022-07-29

Family

ID=67262769

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1905159A Active FR3096146B1 (fr) 2019-05-16 2019-05-16 Dispositif de creation d’un champ evanescent a motifs sur une surface et procede associe

Country Status (5)

Country Link
US (1) US20220214556A1 (fr)
EP (1) EP3969954A1 (fr)
JP (1) JP2022532248A (fr)
FR (1) FR3096146B1 (fr)
WO (1) WO2020230051A1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009055216A1 (de) * 2009-12-22 2011-06-30 Carl Zeiss Microlmaging GmbH, 07745 Lumineszenzmikroskopie
JP5594429B2 (ja) 2011-05-06 2014-09-24 株式会社ニコン 構造化照明顕微鏡及び構造化照明観察方法
JP6264377B2 (ja) * 2013-07-17 2018-01-24 株式会社ニコン 構造化照明装置及び構造化照明顕微鏡装置
JP6396137B2 (ja) * 2014-09-18 2018-09-26 オリンパス株式会社 顕微鏡照明装置、顕微鏡照明方法および顕微鏡

Also Published As

Publication number Publication date
JP2022532248A (ja) 2022-07-13
WO2020230051A1 (fr) 2020-11-19
EP3969954A1 (fr) 2022-03-23
US20220214556A1 (en) 2022-07-07
FR3096146A1 (fr) 2020-11-20

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