FR3066113B1 - Procede d’obtention d’un extrait aqueux d’anethum graveolens enrichi en petits arn - Google Patents

Procede d’obtention d’un extrait aqueux d’anethum graveolens enrichi en petits arn Download PDF

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Publication number
FR3066113B1
FR3066113B1 FR1770486A FR1770486A FR3066113B1 FR 3066113 B1 FR3066113 B1 FR 3066113B1 FR 1770486 A FR1770486 A FR 1770486A FR 1770486 A FR1770486 A FR 1770486A FR 3066113 B1 FR3066113 B1 FR 3066113B1
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Prior art keywords
enriched
aqueous extract
obtaining
anethum
graveolens
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FR1770486A
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FR3066113A1 (fr
Inventor
Elodie OGER
Jean-Marie Botto
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ISP Investments LLC
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ISP Investments LLC
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Publication date
Application filed by ISP Investments LLC filed Critical ISP Investments LLC
Priority to FR1770486A priority Critical patent/FR3066113B1/fr
Priority to FR1870189A priority patent/FR3066114B1/fr
Priority to US16/612,365 priority patent/US11248224B2/en
Priority to CN201880031112.XA priority patent/CN110621379B/zh
Priority to PCT/EP2018/060926 priority patent/WO2018206322A1/fr
Priority to EP18721357.4A priority patent/EP3621701A1/fr
Priority to PCT/EP2018/062186 priority patent/WO2018206751A1/fr
Priority to CN201810448184.6A priority patent/CN108852925B/zh
Publication of FR3066113A1 publication Critical patent/FR3066113A1/fr
Priority to HK18116193.1A priority patent/HK1257057A1/zh
Application granted granted Critical
Publication of FR3066113B1 publication Critical patent/FR3066113B1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/007—Preparations for dry skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/002—Preparations for repairing the hair, e.g. hair cure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Birds (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

L'invention concerne un procédé d'obtention d'un extrait aqueux d'Anethum graveolens enrichi en petits ARN d'une longueur d'au maximum 150 nucléotides à partir d'une matière végétale comprenant les étapes suivantes a) on met en présence les parties aériennes d'aneth dans de l'eau ; b) on ajoute de l'acide éthylène diamine tétraacétique (EDTA) tétrasodique à un pH compris entre 10,5 et 11 ; c) on ajuste ensuite le pH du mélange obtenu en b) à une valeur comprise entre 6 et 8 ; d) on purifie le mélange obtenu en c) ; et e) on contrôle le pH et on le réajuste si nécessaire entre 6 et 8. L'invention concerne également un extrait aqueux d'Anethum graveolens, enrichi en petits ARN d'une longueur d'au maximum 150 nucléotides, obtenu par le procédé, ainsi que les compositions cosmétique comprenant un tel extrait et leurs utilisations cosmétiques pour améliorer l'aspect et l'hydratation de la peau et lutter contre les signes du vieillissement cutané.
FR1770486A 2017-05-12 2017-05-12 Procede d’obtention d’un extrait aqueux d’anethum graveolens enrichi en petits arn Active FR3066113B1 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
FR1770486A FR3066113B1 (fr) 2017-05-12 2017-05-12 Procede d’obtention d’un extrait aqueux d’anethum graveolens enrichi en petits arn
FR1870189A FR3066114B1 (fr) 2017-05-12 2018-02-22 Composition comprenant un extrait aqueux d’anethum graveolens enrichi en petits arn et ses utilisations cosmetiques
CN201880031112.XA CN110621379B (zh) 2017-05-12 2018-04-27 获得莳萝的小rna富集水性提取物的方法
PCT/EP2018/060926 WO2018206322A1 (fr) 2017-05-12 2018-04-27 Procede d'obtention d'un extrait aqueux d'anethum graveolens enrichi en petits arn
US16/612,365 US11248224B2 (en) 2017-05-12 2018-04-27 Method for obtaining a small RNA-enriched aqueous extract of Anethum graveolens
EP18721357.4A EP3621701A1 (fr) 2017-05-12 2018-04-27 Procede d'obtention d'un extrait aqueux d'anethum graveolens enrichi en petits arn
PCT/EP2018/062186 WO2018206751A1 (fr) 2017-05-12 2018-05-11 Composition comprenant un extrait aqueux d'anethum graveolens enrichi en petits arn et ses utilisations cosmetiques
CN201810448184.6A CN108852925B (zh) 2017-05-12 2018-05-11 含有富含小rna的莳萝水性提取物的组合物及其在化妆品中的用途
HK18116193.1A HK1257057A1 (zh) 2017-05-12 2018-12-18 含有富含小rna的蒔蘿水性提取物的組合物及其在化妝品中的用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1770486 2017-05-12
FR1770486A FR3066113B1 (fr) 2017-05-12 2017-05-12 Procede d’obtention d’un extrait aqueux d’anethum graveolens enrichi en petits arn

Publications (2)

Publication Number Publication Date
FR3066113A1 FR3066113A1 (fr) 2018-11-16
FR3066113B1 true FR3066113B1 (fr) 2020-06-05

Family

ID=59153208

Family Applications (2)

Application Number Title Priority Date Filing Date
FR1770486A Active FR3066113B1 (fr) 2017-05-12 2017-05-12 Procede d’obtention d’un extrait aqueux d’anethum graveolens enrichi en petits arn
FR1870189A Active FR3066114B1 (fr) 2017-05-12 2018-02-22 Composition comprenant un extrait aqueux d’anethum graveolens enrichi en petits arn et ses utilisations cosmetiques

Family Applications After (1)

Application Number Title Priority Date Filing Date
FR1870189A Active FR3066114B1 (fr) 2017-05-12 2018-02-22 Composition comprenant un extrait aqueux d’anethum graveolens enrichi en petits arn et ses utilisations cosmetiques

Country Status (6)

Country Link
US (1) US11248224B2 (fr)
EP (1) EP3621701A1 (fr)
CN (2) CN110621379B (fr)
FR (2) FR3066113B1 (fr)
HK (1) HK1257057A1 (fr)
WO (2) WO2018206322A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3106754B1 (fr) * 2020-02-04 2022-01-07 Isp Investments Llc Procede d’obtention d’un extrait aqueux de lavande, compositions comprenant un tel extrait et leurs utilisations cosmetiques
CN113876645B (zh) * 2020-07-01 2024-01-05 伽蓝（集团）股份有限公司 一种须松萝提取物及其应用和含其的皮肤外用剂
EP4134439A1 (fr) * 2021-08-11 2023-02-15 Valagro S.p.A. Extrait de plante et leurs utilisations dans l'agriculture

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Publication number Priority date Publication date Assignee Title
FR2817748B1 (fr) 2000-12-13 2003-01-17 Seporga Composition cosmetique et/ou dermatologique contenant un extrait d'artemia salina
FR2827170B1 (fr) 2001-07-13 2004-07-16 Soc Extraction Principes Actif Utilisation de peptides pour augmenter l'adhesion cellulaire
FR2831168B1 (fr) * 2001-10-22 2004-02-06 Rocher Yves Biolog Vegetale Procede d'obtention d'un extrait riche en acides nucleiques a partir d'une matiere vegetale
FR2837098B1 (fr) 2002-03-18 2004-05-28 Vincience Composition cosmetique ou pharmaceutique comprenant des peptides, procedes de traitement et utilisations
FR2841781B1 (fr) 2002-07-03 2005-12-16 Utilisation de peptides pour favoriser la regeneration cutannee
FR2846883B1 (fr) 2002-11-08 2004-12-24 Vincience Composition cosmetique comprenant, comme ingredient actif, au moins un peptide et utilisation de ce peptide
US20100239510A1 (en) * 2009-01-22 2010-09-23 Robert Bao Kim Ha Skin-care composition comprising dill extract
EP2216074A1 (fr) * 2009-02-07 2010-08-11 Cognis IP Management GmbH Extrait de dolichos biflorus à utiliser dans un traitement cosmétique de la peau
US8080265B2 (en) * 2009-02-20 2011-12-20 Johnson & Johnson Consumer Companies, Inc. Compositions and methods for treating signs of skin aging
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
FR2951946B1 (fr) 2009-11-03 2012-05-11 Isp Investments Inc Utilisation d'un hydrolysat peptidique de levure en tant qu'agent actif pour renforcer le cheveu
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US20110117144A1 (en) * 2009-11-18 2011-05-19 Filiberto Palmiro Zadini Topical cosmetic preparation containing elastogenesis inducing substances associated with a systemic absorption retardant
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FR2956818B1 (fr) 2010-02-26 2012-07-20 Isp Investments Inc Utilisation d'un hydrolysat peptidique de lin dans une composition pour apaiser la peau
DE102010028418A1 (de) * 2010-04-30 2011-11-03 Henkel Ag & Co. Kgaa Wäßriges Kosmetikum mit verbessertem Hautgefühl
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CN104479867A (zh) * 2014-11-28 2015-04-01 湖北中烟工业有限责任公司 一种烟用莳萝油的微胶囊纯化方法及其应用
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Also Published As

Publication number Publication date
US11248224B2 (en) 2022-02-15
CN110621379A (zh) 2019-12-27
US20210155921A1 (en) 2021-05-27
FR3066114A1 (fr) 2018-11-16
EP3621701A1 (fr) 2020-03-18
FR3066114B1 (fr) 2019-08-23
CN110621379B (zh) 2022-07-01
WO2018206751A1 (fr) 2018-11-15
FR3066113A1 (fr) 2018-11-16
CN108852925A (zh) 2018-11-23
CN108852925B (zh) 2023-03-17
HK1257057A1 (zh) 2019-10-11
WO2018206322A1 (fr) 2018-11-15

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