FR2485403A1 - Procede et dispositif permettant d'obtenir de fines couches de matiere, notamment de gel - Google Patents
Procede et dispositif permettant d'obtenir de fines couches de matiere, notamment de gel Download PDFInfo
- Publication number
- FR2485403A1 FR2485403A1 FR8112677A FR8112677A FR2485403A1 FR 2485403 A1 FR2485403 A1 FR 2485403A1 FR 8112677 A FR8112677 A FR 8112677A FR 8112677 A FR8112677 A FR 8112677A FR 2485403 A1 FR2485403 A1 FR 2485403A1
- Authority
- FR
- France
- Prior art keywords
- mold
- layer
- parallel
- plate
- process according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000463 material Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims description 40
- 239000012530 fluid Substances 0.000 claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 239000011521 glass Substances 0.000 claims description 18
- 230000008569 process Effects 0.000 claims description 18
- 229920000728 polyester Polymers 0.000 claims description 16
- 238000006073 displacement reaction Methods 0.000 claims description 12
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 229910000077 silane Inorganic materials 0.000 claims description 7
- 239000004793 Polystyrene Substances 0.000 claims description 6
- 229920002223 polystyrene Polymers 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 5
- 230000007246 mechanism Effects 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 4
- 238000006116 polymerization reaction Methods 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 229920000298 Cellophane Polymers 0.000 claims description 3
- 239000004677 Nylon Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 229910052990 silicon hydride Inorganic materials 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000004952 Polyamide Substances 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 230000002209 hydrophobic effect Effects 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920003023 plastic Polymers 0.000 claims description 2
- 229920002647 polyamide Polymers 0.000 claims description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 2
- 230000001846 repelling effect Effects 0.000 claims description 2
- 230000010076 replication Effects 0.000 claims description 2
- 150000001768 cations Chemical class 0.000 claims 2
- 238000002203 pretreatment Methods 0.000 claims 2
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 239000000470 constituent Substances 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 5
- 230000035515 penetration Effects 0.000 description 5
- 238000001962 electrophoresis Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- XDLMVUHYZWKMMD-UHFFFAOYSA-N 3-trimethoxysilylpropyl 2-methylprop-2-enoate Chemical compound CO[Si](OC)(OC)CCCOC(=O)C(C)=C XDLMVUHYZWKMMD-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005562 fading Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 150000004756 silanes Chemical class 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Casting Or Compression Moulding Of Plastics Or The Like (AREA)
- Moulds For Moulding Plastics Or The Like (AREA)
- Moulding By Coating Moulds (AREA)
- Extrusion Moulding Of Plastics Or The Like (AREA)
- Laminated Bodies (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3024288A DE3024288C2 (de) | 1980-06-27 | 1980-06-27 | Verfahren und Vorrichtung zur Herstellung dünner Schichten |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2485403A1 true FR2485403A1 (fr) | 1981-12-31 |
| FR2485403B1 FR2485403B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1983-03-25 |
Family
ID=6105724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8112677A Granted FR2485403A1 (fr) | 1980-06-27 | 1981-06-26 | Procede et dispositif permettant d'obtenir de fines couches de matiere, notamment de gel |
Country Status (7)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0461352A3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1990-06-14 | 1994-03-30 | Hewlett Packard Co |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5853745A (ja) * | 1981-09-28 | 1983-03-30 | Hitachi Ltd | ２次元電気泳動装置 |
| EP0138763B1 (en) * | 1983-10-04 | 1989-01-18 | IntraCel Corporation | Process for moulding gels usable for thin layers electrophoresis |
| US4865707A (en) * | 1986-10-21 | 1989-09-12 | Northeastern University | Capillary gel electrophoresis columns |
| US4865706A (en) * | 1986-10-21 | 1989-09-12 | Northeastern University | High performance microcapillary gel electrophoresis |
| US5665216A (en) * | 1986-10-21 | 1997-09-09 | Northeastern University | Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same |
| US5112460A (en) * | 1986-10-21 | 1992-05-12 | Northeastern University | High performance microcapillary gel electrophoresis |
| US4997537A (en) * | 1986-10-21 | 1991-03-05 | Northeastern University | High performance microcapillary gel electrophoresis |
| US4795541A (en) * | 1987-05-20 | 1989-01-03 | Bios Corporation | Method and apparatus for molding thin gel slabs horizontally with integrally molded large volume sample wells |
| US4909977A (en) * | 1987-05-20 | 1990-03-20 | Bios Corporation | Method for molding thin gel slabs horizontally with integrally molded large volume sample wells |
| US4938992A (en) * | 1988-01-07 | 1990-07-03 | Varian Associates, Inc. | Methods for thermal transfer with a semiconductor |
| US4997606A (en) * | 1988-01-07 | 1991-03-05 | Varian Associates, Inc. | Methods and apparatus for fabricating a high purity thermally-conductive polymer layer |
| JP2558839B2 (ja) * | 1988-03-16 | 1996-11-27 | 株式会社東芝 | 有機薄膜の製造方法及び製膜装置 |
| US5137613A (en) * | 1990-12-20 | 1992-08-11 | Wisconsin Alumni Research Foundation | Horizontal gel electrophoresis apparatus |
| FR2679033B1 (fr) * | 1991-07-09 | 1993-11-05 | Bertin Et Cie | Procede et dispositif de fabrication d'une plaque de gel a membrane microporeuse destinee a la separation et au transfert de macromolecules par electrophorese, et cassette de gel ainsi obtenue. |
| DE19700626A1 (de) | 1997-01-10 | 1998-07-16 | Europ Lab Molekularbiolog | Probenzuführung |
| US5993627A (en) | 1997-06-24 | 1999-11-30 | Large Scale Biology Corporation | Automated system for two-dimensional electrophoresis |
| US6554991B1 (en) | 1997-06-24 | 2003-04-29 | Large Scale Proteomics Corporation | Automated system for two-dimensional electrophoresis |
| US6267579B1 (en) * | 1998-12-23 | 2001-07-31 | Clinical Laboratory Development Group, Inc. | Apparatus for making a gradient gel |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE59392C (de) * | J. GAEDICKE ia Berlin S.W., Ritterstr. 74 | Giefser zum Auftragen von Emulsion | ||
| US3122781A (en) * | 1961-02-20 | 1964-03-03 | Camag Chemie | Applicator for use in chromatographic analysis |
| DE1598713A1 (de) * | 1965-05-13 | 1970-10-08 | Mueszeripari Muevek Lab | Aufstreichgeraet fuer Duennschichtchromatographie |
| DE2056049A1 (de) * | 1969-11-17 | 1971-06-03 | Elevitch F | Verfahren und Vorrichtung zur Her stellung von Dunnfilmelementen fur die Elektrophorese und chemische und ähnliche Analysen |
| DE2106522A1 (de) * | 1970-02-18 | 1971-09-02 | AB Instrumenta, Lund (Schweden) | Vorrichtung zum Aufstreichen einer dünnen Schicht aus einem flussigen oder halbflussigen Material |
| DE1642821A1 (de) * | 1966-09-13 | 1972-03-09 | Elevich Franklin Ray | Vorrichtung fuer die Elektrophorese,fuer chemische und biochemische Analysen u.dgl. |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2269032A (en) * | 1939-10-25 | 1942-01-06 | American Cast Iron Pipe Co | Apparatus for coating and wrapping pipe |
| US3181222A (en) * | 1962-09-27 | 1965-05-04 | Percy W Palmer | Machine for manufacture of prestressed concrete conduit |
| US3635808A (en) * | 1966-09-13 | 1972-01-18 | Franklin R Elevitch | Method and apparatus for forming electrophoresis apparatus and the like |
| US3767560A (en) * | 1966-09-13 | 1973-10-23 | F Elevitch | Method and apparatus for forming electrophoresis apparatus and the like |
| US3647308A (en) * | 1968-10-28 | 1972-03-07 | Joseph J Dues | Apparatus for forming a cored concrete slab |
| US3922125A (en) * | 1969-12-23 | 1975-11-25 | Borge Christensen | Apparatus for forming continuous lengths of construction elements |
| US3766047A (en) * | 1972-09-11 | 1973-10-16 | F Elevitch | Gel for electrophoresis |
| US4142960A (en) * | 1977-09-01 | 1979-03-06 | Terrance Hahn | Slab gel mold and electrophoresis apparatus |
| US4246222A (en) * | 1979-10-22 | 1981-01-20 | Bio-Rad Laboratories, Inc. | Gel slab casting |
| DE3277081D1 (en) * | 1981-07-31 | 1987-10-01 | Shimadzu Corp | Electrophoretic apparatus |
| US4431506A (en) * | 1982-09-07 | 1984-02-14 | E-C Apparatus Corporation | Apparatus for gel electrophoresis |
-
1980
- 1980-06-27 DE DE3024288A patent/DE3024288C2/de not_active Expired
-
1981
- 1981-06-22 US US06/275,894 patent/US4460525A/en not_active Expired - Lifetime
- 1981-06-24 SE SE8103959A patent/SE438626B/sv not_active IP Right Cessation
- 1981-06-24 JP JP56096822A patent/JPS60893B2/ja not_active Expired
- 1981-06-26 CA CA000380706A patent/CA1166811A/en not_active Expired
- 1981-06-26 GB GB8119737A patent/GB2083228B/en not_active Expired
- 1981-06-26 FR FR8112677A patent/FR2485403A1/fr active Granted
-
1984
- 1984-01-27 US US06/574,357 patent/US4533307A/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE59392C (de) * | J. GAEDICKE ia Berlin S.W., Ritterstr. 74 | Giefser zum Auftragen von Emulsion | ||
| US3122781A (en) * | 1961-02-20 | 1964-03-03 | Camag Chemie | Applicator for use in chromatographic analysis |
| DE1598713A1 (de) * | 1965-05-13 | 1970-10-08 | Mueszeripari Muevek Lab | Aufstreichgeraet fuer Duennschichtchromatographie |
| DE1642821A1 (de) * | 1966-09-13 | 1972-03-09 | Elevich Franklin Ray | Vorrichtung fuer die Elektrophorese,fuer chemische und biochemische Analysen u.dgl. |
| DE2056049A1 (de) * | 1969-11-17 | 1971-06-03 | Elevitch F | Verfahren und Vorrichtung zur Her stellung von Dunnfilmelementen fur die Elektrophorese und chemische und ähnliche Analysen |
| FR2069512A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1969-11-17 | 1971-09-03 | Elevitch Franklin Ray | |
| DE2106522A1 (de) * | 1970-02-18 | 1971-09-02 | AB Instrumenta, Lund (Schweden) | Vorrichtung zum Aufstreichen einer dünnen Schicht aus einem flussigen oder halbflussigen Material |
| FR2078591A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1970-02-18 | 1971-11-05 | Instrumenta Ab |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0461352A3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1990-06-14 | 1994-03-30 | Hewlett Packard Co |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2485403B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1983-03-25 |
| US4460525A (en) | 1984-07-17 |
| GB2083228A (en) | 1982-03-17 |
| SE8103959L (sv) | 1982-02-12 |
| DE3024288C2 (de) | 1982-03-25 |
| JPS60893B2 (ja) | 1985-01-10 |
| SE438626B (sv) | 1985-04-29 |
| JPS5736622A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1982-02-27 |
| CA1166811A (en) | 1984-05-08 |
| DE3024288A1 (de) | 1982-01-21 |
| US4533307A (en) | 1985-08-06 |
| GB2083228B (en) | 1984-11-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |