FR2434394A1 - Produit et procede pour la coloration de frottis cellulaires - Google Patents
Produit et procede pour la coloration de frottis cellulairesInfo
- Publication number
- FR2434394A1 FR2434394A1 FR7918692A FR7918692A FR2434394A1 FR 2434394 A1 FR2434394 A1 FR 2434394A1 FR 7918692 A FR7918692 A FR 7918692A FR 7918692 A FR7918692 A FR 7918692A FR 2434394 A1 FR2434394 A1 FR 2434394A1
- Authority
- FR
- France
- Prior art keywords
- product
- frottis
- coloring
- cellular
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000001413 cellular effect Effects 0.000 title 1
- 238000004040 coloring Methods 0.000 title 1
- 239000000975 dye Substances 0.000 abstract 3
- HLUCICHZHWJHLL-UHFFFAOYSA-N Haematein Natural products C12=CC=C(O)C(O)=C2OCC2(O)C1=C1C=C(O)C(=O)C=C1C2 HLUCICHZHWJHLL-UHFFFAOYSA-N 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- HNNSUZPWERIYIL-UHFFFAOYSA-N chembl1730100 Chemical compound O1CC2(O)CC3=CC(O)=C(O)C=C3C2=C2C1=C(O)C(=O)C=C2 HNNSUZPWERIYIL-UHFFFAOYSA-N 0.000 abstract 1
- 230000002380 cytological effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 abstract 1
- 210000004392 genitalia Anatomy 0.000 abstract 1
- 239000002184 metal Substances 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
- AAAQKTZKLRYKHR-UHFFFAOYSA-N triphenylmethane Chemical compound C1=CC=CC=C1C(C=1C=CC=CC=1)C1=CC=CC=C1 AAAQKTZKLRYKHR-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Produit et procédé pour la coloration de frottis cellulaires. Le produit contient de l'hématéine, un colorant de triphénylméthane et un colorant de fluorescéine. Les prélèvements sont fixés, traités par une solution de sel métallique puis par la combinaison de colorants. Le produit et le procédé selon l'invention peuvent être utilisés au laboratoire pour le diagnostic cytologique et en particulier pour la détection des cancers génitaux.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19782832491 DE2832491A1 (de) | 1978-07-24 | 1978-07-24 | Mittel und verfahren zur anfaerbung von zellabstrichen |
Publications (1)
Publication Number | Publication Date |
---|---|
FR2434394A1 true FR2434394A1 (fr) | 1980-03-21 |
Family
ID=6045264
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7918692A Withdrawn FR2434394A1 (fr) | 1978-07-24 | 1979-07-19 | Produit et procede pour la coloration de frottis cellulaires |
Country Status (7)
Country | Link |
---|---|
JP (1) | JPS5518999A (fr) |
DE (1) | DE2832491A1 (fr) |
FI (1) | FI792304A (fr) |
FR (1) | FR2434394A1 (fr) |
GB (1) | GB2026015B (fr) |
IT (1) | IT1118123B (fr) |
SE (1) | SE7906296L (fr) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IT1191052B (it) * | 1982-10-28 | 1988-02-24 | Sclavo Inst Sieroterapeut | Reattivo per la determinazione delle beta-lipoproteine. |
US4487839A (en) * | 1983-01-05 | 1984-12-11 | Ortho Diagnostic Systems Inc. | Immunoassay methods employing patterns for the detection of soluble and cell surface antigens |
ES2278566T3 (es) * | 1994-10-20 | 2007-08-16 | Sysmex Corporation | Reactivo y metodo para analizar componentes solidos en la orina. |
DE10255601C5 (de) * | 2002-10-14 | 2017-10-19 | Fluoron Gmbh | Verwendung eines Triphenylmethanfarbstoffs zur Herstellung eines Färbemittels bei der Netzhaut- und Glaskörperchirurgie |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH294394A (de) * | 1950-05-02 | 1953-11-15 | Inc Technicon Chemical Company | Färbeflüssigkeit für tierisches Gewebe. |
DE1044459B (de) * | 1956-11-15 | 1958-11-20 | Merck Ag E | Verfahren zum Anfaerben von Faserstoffen zum Zwecke der Durchfuehrung von Faserstoffanalysen und dazu verwendete Mittel |
DE1617641A1 (de) * | 1967-06-16 | 1971-03-25 | Merck Patent Gmbh | Farbstoffzubereitungen zum Faerben von Blutpraeparaten und Verfahren zu ihrer Herstellung |
FR2110119A5 (fr) * | 1970-10-30 | 1972-05-26 | Gen Electric |
-
1978
- 1978-07-24 DE DE19782832491 patent/DE2832491A1/de not_active Withdrawn
-
1979
- 1979-07-19 FR FR7918692A patent/FR2434394A1/fr not_active Withdrawn
- 1979-07-23 SE SE7906296A patent/SE7906296L/ not_active Application Discontinuation
- 1979-07-23 FI FI792304A patent/FI792304A/fi not_active Application Discontinuation
- 1979-07-23 GB GB7925561A patent/GB2026015B/en not_active Expired
- 1979-07-23 IT IT49831/79A patent/IT1118123B/it active
- 1979-07-24 JP JP9332779A patent/JPS5518999A/ja active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH294394A (de) * | 1950-05-02 | 1953-11-15 | Inc Technicon Chemical Company | Färbeflüssigkeit für tierisches Gewebe. |
DE1044459B (de) * | 1956-11-15 | 1958-11-20 | Merck Ag E | Verfahren zum Anfaerben von Faserstoffen zum Zwecke der Durchfuehrung von Faserstoffanalysen und dazu verwendete Mittel |
DE1617641A1 (de) * | 1967-06-16 | 1971-03-25 | Merck Patent Gmbh | Farbstoffzubereitungen zum Faerben von Blutpraeparaten und Verfahren zu ihrer Herstellung |
FR2110119A5 (fr) * | 1970-10-30 | 1972-05-26 | Gen Electric |
Also Published As
Publication number | Publication date |
---|---|
IT7949831A0 (it) | 1979-07-23 |
GB2026015B (en) | 1982-08-18 |
IT1118123B (it) | 1986-02-24 |
SE7906296L (sv) | 1980-01-26 |
FI792304A (fi) | 1980-01-25 |
JPS5518999A (en) | 1980-02-09 |
DE2832491A1 (de) | 1980-02-07 |
GB2026015A (en) | 1980-01-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Frankel et al. | A simplified Chatton-Lwoff silver impregnation procedure for use in experimental studies with ciliates | |
Cohen et al. | A cyanine dye distinguishes between cycling and non-cycling fibroblasts | |
GB1492556A (en) | Method for fluorescence analysis of white cells | |
FR2434394A1 (fr) | Produit et procede pour la coloration de frottis cellulaires | |
DeLamater et al. | The chemistry of the Feulgen reaction and related histo-and cytochemical methods | |
FR2393834A1 (fr) | Nouveaux colorants reactifs contenant le residu de l'acide 1-hydroxy-7-amino-8-(5'-amino-2',4'-disulfophenylazo) naphtalene-3,6-disulfonique, leur procede de production et leurs applications tinctoriales | |
FR2442873A1 (fr) | Colorants disazoiques, leur procede de production et leur application a la teinture de matieres cellulosiques | |
DE2649902B2 (de) | Autoradiografisches Verfahren zur Bestimmung physiologischer Veränderungen in Geweben | |
Schnedl et al. | Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15, and the Y by D 287/170 | |
FR2415648A1 (fr) | Colorants heterocycliques, leur procede de production et leur application a la teinture et l'impression de matieres fibreuses | |
Conklin et al. | A quantitative procedure for the determination of nitrofurantoin in whole blood and plasma | |
FR2438112A1 (fr) | Procede et preparations tinctoriales pour la teinture et l'impression de fibres cellulosiques | |
CN108586448A (zh) | 一种喹啉鎓盐型化合物及其应用 | |
Stockert | The horse eosinophil as a model leucocyte for morphological and cytochemical studies | |
Leemann et al. | Selective excitation of mithramycin or DAPI fluorescence on double-stained cell nuclei and chromosomes | |
Schwalbach et al. | Differentiated staining of osmium tetroxide-fixed, Vestopal-W-embedded tissue in thin sections | |
GB1473945A (en) | Method for preparing slides for blood evaluation | |
JPH0331388B2 (fr) | ||
Suzuki et al. | Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy | |
Crescitelli | Extraction of visual pigments with certain alkyl phenoxy polyethoxy ethanol surface-active compounds | |
Fey | Eriochrome black, a means for reduction of nonspecificity in immunofluorescence | |
FR2423520A1 (fr) | Nouveaux colorants reactifs, leur procede de production et leurs applications tinctoriales | |
Juarranz et al. | Fluorescent porphyrin counterstaining of chromatin DNA in conjunction with immunofluorescence methods using FITC‐labelled antibodies | |
FR2397441A1 (fr) | Procede pour la fabrication de colorants pour cuve et leur utilisation | |
Kay | A mixture of methyl green and pyronin for cell fractionation studies |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |