FR2415809A1 - Determinations immunologiques utilisant des fragments f(ab')2 - Google Patents

Determinations immunologiques utilisant des fragments f(ab')2

Info

Publication number
FR2415809A1
FR2415809A1 FR7901537A FR7901537A FR2415809A1 FR 2415809 A1 FR2415809 A1 FR 2415809A1 FR 7901537 A FR7901537 A FR 7901537A FR 7901537 A FR7901537 A FR 7901537A FR 2415809 A1 FR2415809 A1 FR 2415809A1
Authority
FR
France
Prior art keywords
fragments
determinations
immunoassays
liquid
clq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7901537A
Other languages
English (en)
Other versions
FR2415809B1 (fr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Technicon Instruments Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technicon Instruments Corp filed Critical Technicon Instruments Corp
Publication of FR2415809A1 publication Critical patent/FR2415809A1/fr
Application granted granted Critical
Publication of FR2415809B1 publication Critical patent/FR2415809B1/fr
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/563—Immunoassay; Biospecific binding assay; Materials therefor involving antibody fragments
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne des déterminations immunologiques utilisant des fragments F(ab')2 . Pour effectuer des déterminations immunologiques d'antigènes ou d'haptènes, on utilise comme anticorps, au lieu d'une immunoglobuline G, ses fragments F(ab')2 . On évite ainsi les interférences provoquées par le RF et le Clq endogènes présents dans le liquide à analyser, sans qu'il soit nécessaire de soumettre ce liquide à un prétraitement pour éliminer ou inactiver le RF ou le Clq; l'invention vise également un réactif utile pour de telles déterminations, constitué de fragments F(ab')2 insolubilisés sur un substrat insoluble dans l'eau, en particulier un substrat en particules. L'invention s'applique à des déterminations immunologiques de divers types, y compris des agglutinations de latex et des déterminations par fixation compétitive.
FR7901537A 1978-01-26 1979-01-22 Determinations immunologiques utilisant des fragments f(ab')2 Expired FR2415809B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB323778 1978-01-26

Publications (2)

Publication Number Publication Date
FR2415809A1 true FR2415809A1 (fr) 1979-08-24
FR2415809B1 FR2415809B1 (fr) 1985-04-12

Family

ID=9754542

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7901537A Expired FR2415809B1 (fr) 1978-01-26 1979-01-22 Determinations immunologiques utilisant des fragments f(ab')2

Country Status (12)

Country Link
US (1) US4397960A (fr)
JP (2) JPS54119292A (fr)
AU (1) AU524656B2 (fr)
BE (1) BE873673A (fr)
CA (1) CA1118345A (fr)
CH (1) CH639208A5 (fr)
DE (1) DE2902676C2 (fr)
FR (1) FR2415809B1 (fr)
GB (1) GB2013211B (fr)
IT (1) IT1119257B (fr)
NL (1) NL7900586A (fr)
SE (1) SE445393B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0046786A1 (fr) * 1980-02-22 1982-03-10 Scripps Clinic And Research Foundation Essai anti-c3 en phase solide de detection de complexes immunologiques

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2013211B (en) * 1978-01-26 1982-06-30 Technicon Instr Immunoassays using f(ab')2 fragments
JPS54139595A (en) * 1978-04-20 1979-10-30 Mitsubishi Chem Ind Reagent for detecting antigen
AU543007B2 (en) * 1980-04-15 1985-03-28 Technicon Instruments Corportion Agglutination immunoassay
JPS56162055A (en) * 1980-05-19 1981-12-12 Eiken Kagaku Kk Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles
EP0051985B2 (fr) * 1980-11-07 1989-12-27 International Institute Of Cellular And Molecular Pathology (Icp) Essai immunologique de protéines
JPS57182168A (en) * 1981-05-02 1982-11-09 Mitsubishi Chem Ind Ltd Immunochemical reagent
DE3266967D1 (en) * 1982-01-05 1985-11-21 Int Inst Cellular Molecul Path Method of immunoassay
DE3225027A1 (de) * 1982-07-05 1984-01-05 Boehringer Mannheim Gmbh, 6800 Mannheim Immunchemisches messverfahren
DE3366740D1 (en) * 1982-08-06 1986-11-13 Int Inst Cellular Molecul Path Particle agglutination assay of antigens
JPS59202064A (ja) * 1983-04-30 1984-11-15 Fujirebio Inc 抗原決定基具有物質を測定する方法
US4623620A (en) * 1983-08-02 1986-11-18 Stichting Vrienden Van De Stichting Dr. Karl Landsteiner Enucleated granulocytes, their preparation and use
DE3331627A1 (de) * 1983-09-01 1985-03-21 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Verfahren zur immunologischen bestimmung fuer proteine in koerperfluessigkeiten, unter verwendung monovalenter antikoerperfragmente
US4551426A (en) * 1983-10-03 1985-11-05 E. I. Du Pont De Nemours And Company Heterogeneous immunoassay for digoxin using ouabain as a separation means
DE3400027A1 (de) * 1984-01-02 1985-07-18 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur bestimmung eines polyvalenten antigens und reagenz hierfuer
BE899664A (fr) * 1984-05-15 1984-08-31 Inst Internat De Pathologie Ce Procede de dosage immunologique d'une substance dans un echantillon liquide.
CA1261743A (fr) * 1984-07-23 1989-09-26 Shai Inbar Produit pour les essais de diagnostic biologiques et procede utilisant des fragments fab marques
GB8422452D0 (en) * 1984-09-05 1984-10-10 Serono Diagnostics Ltd Assay
JPS6175262A (ja) * 1984-09-21 1986-04-17 Teijin Ltd 免疫複合体測定用試薬及びそれを用いた免疫複合体の測定法
US4560504A (en) * 1984-12-06 1985-12-24 Uop Inc. Carboxyl anchored immobilized antibodies
GB2171999A (en) * 1985-03-04 1986-09-10 Iq Antibodies
DE3638767A1 (de) * 1986-11-13 1988-05-26 Behringwerke Ag Laktoferrin enthaltendes inkubationsmedium fuer festphasenimmunometrisches verfahren und seine verwendung
US4921787A (en) * 1987-05-01 1990-05-01 Cambridge Bioscience Corporation Detection of antibodies to human immunodeficiency virus by agglutination of antigen coated latex
US4791067A (en) * 1987-06-25 1988-12-13 Fisher Scientific Co. Agglutination immunoassay for hapten involving monoclonal antibody of IgA class reagent
US4829011A (en) * 1987-08-27 1989-05-09 Biotrack, Inc. Agglutination assay
GB8800293D0 (en) * 1988-01-07 1988-02-10 Int Inst Cellular Molecul Path Antibodies attached to solid surface
US4914041A (en) * 1988-02-12 1990-04-03 Beckman Instruments, Inc. Reagent and method for detecting rheumatoid factor
US5389523A (en) * 1988-05-31 1995-02-14 The United States Of Americas, As Represented By The Secretary Of Commerce Liposome immunoanalysis by flow injection assay
DE3829245A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur bestimmung einer spezifisch bindefaehigen substanz
FR2654836B1 (fr) * 1989-11-17 1994-01-28 Biotrol Sa Laboratoires Appareil d'execution automatique d'un immunodosage en plusieurs etapes successives d'au moins une substance biologique dans une pluralite d'echantillons biologiques, procede et reactif mettant en óoeuvre ledit appareil.
US5231004A (en) * 1990-05-11 1993-07-27 Eastman Kodak Company Use of heme-containing proteins as stabilizers for enzyme-labeled immunoreactants
CA2038260A1 (fr) * 1990-05-11 1991-11-12 John C. Mauck Utilisation d'un fragment d'antigene marque par un enzyme dans la determination d'antigenes de chlamydia ou de gonocoques
US5585278A (en) * 1994-10-27 1996-12-17 Bayer Corporation Method for coupling antibody to novel latex substrate and its use in immunoassay
ATE201100T1 (de) * 1996-12-18 2001-05-15 Diagnostische Forsch Stiftung Synthetische partikel als agglutinationsreagenzien
GB9705376D0 (en) * 1997-03-14 1997-04-30 Harris William J Antibody fragment formulations and assay formats for detecting the presence an d concentration of analytes
AU7977098A (en) * 1997-06-17 1999-01-25 Palingen, Inc. Method for diagnosing systemic lupus erythematosus
US6977142B2 (en) * 2003-09-23 2005-12-20 Agy Therapeutics, Inc. High-throughput turbidometric assay for screening inhibitors of protein disulfide isomerase activity
US20080108147A1 (en) * 2006-11-03 2008-05-08 Tie Wei Reduction of non-specific binding in immunoassays
EP3531130B1 (fr) 2016-10-19 2022-04-13 Eiken Kagaku Kabushiki Kaisha Procédé de dosage immunologique et réactif de dosage
CN113228265B (zh) 2019-07-03 2024-09-24 富士电机株式会社 半导体组件的电路构造
US20230122067A1 (en) 2020-03-26 2023-04-20 Denka Company Limited REAGENT COMPRISING ANTIBODY FROM WHICH PART OF Fc REGION IS DELETED
WO2024038338A1 (fr) 2022-08-15 2024-02-22 Solventum Intellectual Properties Company Substrats poreux fonctionnalisés et leur utilisation pour la détection d'analytes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2123561A1 (fr) * 1968-12-16 1972-09-08 Abbott Lab
US4070246A (en) * 1976-04-09 1978-01-24 Abbott Laboratories Reactive matrices
DE2643207A1 (de) * 1976-09-25 1978-03-30 Behringwerke Ag Verfahren zum nachweis und zur bestimmung eines partners einer immunologischen reaktion

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3646346A (en) * 1968-12-26 1972-02-29 Pharmacia Ab Antibody-coated tube system for radioimmunoassay
US4115534A (en) * 1976-08-19 1978-09-19 Minnesota Mining And Manufacturing Company In vitro diagnostic test
DE2644249A1 (de) * 1976-09-30 1978-04-06 Abbott Lab Reaktive matrices
JPS5344622A (en) * 1976-09-30 1978-04-21 Mochida Pharm Co Ltd Immunologically measuring method
GB2013688B (en) * 1978-01-26 1982-06-30 Technicon Instr Insolubilised proteins and immunoassays utilising them
GB2013211B (en) * 1978-01-26 1982-06-30 Technicon Instr Immunoassays using f(ab')2 fragments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2123561A1 (fr) * 1968-12-16 1972-09-08 Abbott Lab
US4070246A (en) * 1976-04-09 1978-01-24 Abbott Laboratories Reactive matrices
DE2643207A1 (de) * 1976-09-25 1978-03-30 Behringwerke Ag Verfahren zum nachweis und zur bestimmung eines partners einer immunologischen reaktion

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol.81, no.5, 5 août 1974, page 312, résumé no. 24029q, COLUMBUS, Ohio (US) *
JOURNAL OF ORGANIC CHEMISTRY, vol.41, no.8, 1976, EASTON (US) *
THE JOURNAL OF IMMUNOLOGY, vol.116, no.6, juin 1976, The Williams & Wilkins Co., BALTIMORE (US) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0046786A1 (fr) * 1980-02-22 1982-03-10 Scripps Clinic And Research Foundation Essai anti-c3 en phase solide de detection de complexes immunologiques
EP0046786A4 (fr) * 1980-02-22 1982-07-13 Scripps Clinic Res Essai anti-c3 en phase solide de detection de complexes immunologiques.

Also Published As

Publication number Publication date
GB2013211A (en) 1979-08-08
IT1119257B (it) 1986-03-10
US4397960A (en) 1983-08-09
DE2902676C2 (de) 1996-10-17
FR2415809B1 (fr) 1985-04-12
SE445393B (sv) 1986-06-16
SE7900661L (sv) 1979-07-27
GB2013211B (en) 1982-06-30
NL7900586A (nl) 1979-07-30
JPS54119292A (en) 1979-09-17
JPS6338668B2 (fr) 1988-08-01
AU4356579A (en) 1979-08-02
AU524656B2 (en) 1982-09-30
BE873673A (fr) 1979-07-24
CA1118345A (fr) 1982-02-16
JPH02276968A (ja) 1990-11-13
JPH0521186B2 (fr) 1993-03-23
IT7967165A0 (it) 1979-01-25
CH639208A5 (de) 1983-10-31
DE2902676A1 (de) 1979-08-02

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