ES8503855A1 - Procedimiento de inmunoanalisis homogeneo para determinar un analito en una muestra de ensayo. - Google Patents

Procedimiento de inmunoanalisis homogeneo para determinar un analito en una muestra de ensayo.

Info

Publication number
ES8503855A1
ES8503855A1 ES520777A ES520777A ES8503855A1 ES 8503855 A1 ES8503855 A1 ES 8503855A1 ES 520777 A ES520777 A ES 520777A ES 520777 A ES520777 A ES 520777A ES 8503855 A1 ES8503855 A1 ES 8503855A1
Authority
ES
Spain
Prior art keywords
analyte
labeled
monoclonal anti
label
homogeneous immunoassay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES520777A
Other languages
English (en)
Other versions
ES520777A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES8503855A1 publication Critical patent/ES8503855A1/es
Publication of ES520777A0 publication Critical patent/ES520777A0/es
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO DE INMUNOANALISIS HOMOGENEO PARA DETERMINAR UN ANALITO EN UNA MUESTRA DE ENSAYO.COMPRENDE: A) COMBINAR LA MUESTRA DE ENSAYO CON UN PREPARADO DE ANTI-ANALITO MONOCLONAL MARCADO, SIENDO EL ANTI-ANALITO MONOCLONAL EL UNICO COMPONENTE MARCADO, Y CON UN CONJUGADO MACROMOLECULAR QUE COMPRENDE EPITOPOS LIGABLES POR EL ANTI-ANALITO MONOCLONAL MARCADO, PARA OBTENER UNA SEN/AL OPTICA DETECTABLE Y MEDIBLE; Y B) MEDIR LA SEN/AL OPTICA PRODUCIDA POR FLUORESCENCIA Y COMPARAR LA MEDIDA HALLADA CON SEN/ALES PRODUCIDAS CUANDO SE ANALIZAN MUESTRAS DE REFERENCIA QUE CONTIENE CANTIDADES CONOCIDAS DE ANALITO.
ES520777A 1982-03-18 1983-03-18 Procedimiento de inmunoanalisis homogeneo para determinar un analito en una muestra de ensayo. Granted ES520777A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35961082A 1982-03-18 1982-03-18

Publications (2)

Publication Number Publication Date
ES8503855A1 true ES8503855A1 (es) 1985-03-16
ES520777A0 ES520777A0 (es) 1985-03-16

Family

ID=23414570

Family Applications (1)

Application Number Title Priority Date Filing Date
ES520777A Granted ES520777A0 (es) 1982-03-18 1983-03-18 Procedimiento de inmunoanalisis homogeneo para determinar un analito en una muestra de ensayo.

Country Status (9)

Country Link
EP (1) EP0088974A3 (es)
JP (1) JPS58176550A (es)
AU (1) AU549033B2 (es)
CA (1) CA1186622A (es)
DK (1) DK124083A (es)
ES (1) ES520777A0 (es)
GR (1) GR77159B (es)
IL (1) IL67289A (es)
NO (1) NO830712L (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3483620D1 (de) * 1983-03-11 1991-01-03 Fujirebio Kk Verfahren zur bestimmung von liganden.
US4692404A (en) * 1983-11-18 1987-09-08 Fujirebio Kabushiki Kaisha Method of measuring biological ligand by the use of enzymes
JPS60233555A (ja) * 1984-01-05 1985-11-20 オ−ソ・ダイアグノステイツク・システムズ・インコ−ポレ−テツド 螢光エネルギ−移動を使用する抗遺伝子型検定
JPS60228421A (ja) * 1984-04-27 1985-11-13 Shionogi & Co Ltd モノクロ−ナル抗ヒトＩｇＧ抗体およびその製造法
EP0161868B1 (en) * 1984-05-11 1992-11-04 BioWhittaker, Inc. Fluorometric assay of igg4
JPS6222064A (ja) * 1985-07-23 1987-01-30 Teijin Ltd 第８因子凝固活性抗原の測定方法
ES8606501A1 (es) * 1985-07-31 1986-04-01 Abello Alergia & Inmunologia Un metodo para determinar la presencia de igg especifica en sueros humanos
JPH02213766A (ja) * 1989-02-15 1990-08-24 Mochida Pharmaceut Co Ltd 免疫測定用試薬および免疫測定器具
GB8927503D0 (en) * 1989-12-04 1990-02-07 Kronem Systems Inc Enzyme-amplified lanthanide chelate luminescence
JP2786336B2 (ja) * 1991-03-04 1998-08-13 富士写真フイルム株式会社 免疫分析要素および免疫分析方法
US5447846A (en) * 1992-07-17 1995-09-05 Fuji Photo Film C., Ltd. Homogeneous Immunoassay process using covalent conjugate of enzyme and plural monoclonal antibodies for different epitopes on analyte
EP0957365B1 (en) * 1997-09-05 2006-02-01 Matsushita Electric Industrial Co., Ltd. Fluorescence polarization method

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE792798A (fr) * 1971-12-16 1973-06-15 Radiochemical Centre Ltd Procede et reactifs destines notamment a des titrages radio-immunologiques
US3935074A (en) * 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
IN142734B (es) * 1975-04-28 1977-08-20 Miles Lab
IT1105734B (it) * 1977-07-14 1985-11-04 Syva Co Prova di legame di competizione di antienzima omogeneo
DE3177290T2 (de) * 1980-04-09 1993-04-15 British Tech Group Monoklonale antikoerper gegen hepatitis-b-virus.
CH642458A5 (en) * 1980-04-25 1984-04-13 Hoffmann La Roche Immunological method
IL64574A (en) * 1981-04-27 1986-01-31 Syva Co Method for determining the presence of an analyte by determination of the amount of fluorescence in competitive protein binding assays
GB2102946B (en) * 1981-06-30 1984-11-28 Wellcome Found Enzyme immunoassay

Also Published As

Publication number Publication date
CA1186622A (en) 1985-05-07
EP0088974A2 (en) 1983-09-21
AU9076882A (en) 1983-09-22
JPS58176550A (ja) 1983-10-17
DK124083D0 (da) 1983-03-17
IL67289A0 (en) 1983-03-31
IL67289A (en) 1985-12-31
GR77159B (es) 1984-09-07
EP0088974A3 (en) 1986-05-28
ES520777A0 (es) 1985-03-16
NO830712L (no) 1983-09-19
DK124083A (da) 1983-09-19
AU549033B2 (en) 1986-01-09

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