JPS56162055A - Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles - Google Patents

Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Info

Publication number
JPS56162055A
JPS56162055A JP6543480A JP6543480A JPS56162055A JP S56162055 A JPS56162055 A JP S56162055A JP 6543480 A JP6543480 A JP 6543480A JP 6543480 A JP6543480 A JP 6543480A JP S56162055 A JPS56162055 A JP S56162055A
Authority
JP
Japan
Prior art keywords
latex particles
immunoglobulin
fragments
sensitizing
antigens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP6543480A
Other languages
Japanese (ja)
Other versions
JPS6363863B2 (en
Inventor
Noriyuki Tsubota
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eiken Chemical Co Ltd
Original Assignee
Eiken Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Chemical Co Ltd filed Critical Eiken Chemical Co Ltd
Priority to JP6543480A priority Critical patent/JPS56162055A/en
Publication of JPS56162055A publication Critical patent/JPS56162055A/en
Publication of JPS6363863B2 publication Critical patent/JPS6363863B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Abstract

PURPOSE:To determine the existing quantity of an antigen easily with good accuracy by sensitizing a mixture of immunoglobulin F(ab')2 fragments and Fc fragments with latex particles, and allowing the resultant sensitizing latex particles and the antigens in humors to react. CONSTITUTION:A mixture of immunoglobulin F(ab')2 fragments and Fc fragments obtained by enzyme decomposition of immunoglobulin with Pepsin is sensitized with latex particles of 0.1-1mum average grain sizes. Thence, the resultant sensitizing latex particles and humor sample are mixed, and the existing quantity of the antigens is determined by such a method of deciding the degrees of latex flocculation caused by the reaction of the antigens in the sample and the immunoglobulin F(ab')2 sections by naked eyes or irradiating light of 0.3-1.8mum wavelengths and measuring the absorbancy thereof or the intensity of the scattered light. Thereby, the measurement is made without receiving the interference of RA factors or the like in spite of their existance in the sample.
JP6543480A 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles Granted JPS56162055A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP6543480A JPS56162055A (en) 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP6543480A JPS56162055A (en) 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Publications (2)

Publication Number Publication Date
JPS56162055A true JPS56162055A (en) 1981-12-12
JPS6363863B2 JPS6363863B2 (en) 1988-12-08

Family

ID=13286994

Family Applications (1)

Application Number Title Priority Date Filing Date
JP6543480A Granted JPS56162055A (en) 1980-05-19 1980-05-19 Method for determining immunity using immunoglobulin fragment mixture sensitizing latex particles

Country Status (1)

Country Link
JP (1) JPS56162055A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63184062A (en) * 1986-09-30 1988-07-29 アンディシア　ディアグノスティック Analyzing method and device for critically important immunological reactive substance
CN102749445A (en) * 2012-06-29 2012-10-24 中国人民解放军军事医学科学院军事兽医研究所 Method for improving rabies neutralizing antibody detection sensitivity in latex agglutination test

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5362826A (en) * 1976-11-10 1978-06-05 Hoffmann La Roche Quantitative determination of immunologically active substance in analysing sample
JPS5444009A (en) * 1977-07-15 1979-04-07 Behringwerke Ag Immune globulin decomposed substance bound to carrier and use thereof in immunological analytic method
JPS54109494A (en) * 1978-02-16 1979-08-28 Teikoku Hormone Mfg Co Ltd Method of measuring antigennantibody reaction
JPS54119292A (en) * 1978-01-26 1979-09-17 Technicon Instr Immunity examination by f*ab**2

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5362826A (en) * 1976-11-10 1978-06-05 Hoffmann La Roche Quantitative determination of immunologically active substance in analysing sample
JPS5444009A (en) * 1977-07-15 1979-04-07 Behringwerke Ag Immune globulin decomposed substance bound to carrier and use thereof in immunological analytic method
JPS54119292A (en) * 1978-01-26 1979-09-17 Technicon Instr Immunity examination by f*ab**2
JPS54109494A (en) * 1978-02-16 1979-08-28 Teikoku Hormone Mfg Co Ltd Method of measuring antigennantibody reaction

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63184062A (en) * 1986-09-30 1988-07-29 アンディシア　ディアグノスティック Analyzing method and device for critically important immunological reactive substance
CN102749445A (en) * 2012-06-29 2012-10-24 中国人民解放军军事医学科学院军事兽医研究所 Method for improving rabies neutralizing antibody detection sensitivity in latex agglutination test

Also Published As

Publication number Publication date
JPS6363863B2 (en) 1988-12-08

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