FI96116B - Förfarande för att framställa eglinmutanter med proteasinhibitoraktivitet - Google Patents
Förfarande för att framställa eglinmutanter med proteasinhibitoraktivitet Download PDFInfo
- Publication number
- FI96116B FI96116B FI891020A FI891020A FI96116B FI 96116 B FI96116 B FI 96116B FI 891020 A FI891020 A FI 891020A FI 891020 A FI891020 A FI 891020A FI 96116 B FI96116 B FI 96116B
- Authority
- FI
- Finland
- Prior art keywords
- eglin
- dna
- formula
- thr
- asp
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/55—Protease inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/855—Proteins from animals other than mammals or birds
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- Plant Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Tropical Medicine & Parasitology (AREA)
- Heart & Thoracic Surgery (AREA)
- Rheumatology (AREA)
- Cardiology (AREA)
- Pain & Pain Management (AREA)
- Pulmonology (AREA)
- Immunology (AREA)
Claims (10)
1. Förfarande för framstälining av föreningar med forxneln I med proteasinhibitoraktivitet R-ThrGluPheGlySerGluLeuLysSerPheProGluValValGlyLysThrVal-AspGlnAlaArgGluTyrPheThrLeuHisTyrProGlnTyrAspVal-W-Phe-LeuProGluGlySerProVa1-X-Y-Z-LeuArgTyrAsnArgValArgVa1-PheTyrAsnProGlyThrAsnVaIValAsnHisVaIProHisValGly-OH (I), i vilken formel R betecknar väte eller acetyl, W är Tyr eller His, X är Thr, Ser eller Pro, Y är Leu, Met, Arg, Lys, Phe, Tyr eller Trp och Z är Asp, Glu, Gin, Asn, Ala, Ser eller Thr, förutsatt att X är skild frän Thr, när Y är Leu och Z är Asp, samt salter av dessa föreningar, kännetecknat därav, att man odlar en transformerad värd-mikroorganism, som innehäller en DNA-sekvens som kodar för en förening med formeln I, och isolerar en förening med formeln I eller ett sait därav.
2. Förfarande enligt patentkravet 1 för framställning av föreningar med formeln I och salter därav, kännetecknat därav, att R är acetyl, W är Tyr och antingen X är Thr, Y är Arg eller Lys och Z är Asp, Glu, Gin, Asn, Ala, Ser eller Thr eller X är Pro, Y är Met och Z är Asp eller X är Thr, Y är Phe, Tyr, Trp eller Met och Z är Asp.
3. Förfarande enligt patentkravet 1 för framställning av föreningar med formeln I och salter därav, kännetecknat därav, att R är acetyl, W är Tyr, X är Thr, Y är Arg och Z är Asp eller Ser.
4. Förfarande enligt patentkravet 1, kännetecknat därav, att man framställer N“-acetyl-(Arg45)-eglin C. Il 96116
5. Förfarande för framställning av en sädan DNA, som innehäller en rekombinant-DNA-sekvens enligt patentkravet 8, kännetecknat därav, att DNAn framstäiIs med en kemlsk syntes eller fragment av DNA framställs med en kemisk syntes och ligeras ihop enzymatiskt i en bestämd ordning eller DNA som kodar för eglin B eller eglin C mutagenise-ras i ett eller flera steg.
6. Förfarande för framställning av en uttryckningsvektor, som innehäller en rekombinant-DNA-sekvens enligt patentkravet 8 som är under kontroll av en uttryckningskont-rollsekvens, kännetecknat därav, att en DNA-sekvens enligt patentkravet 8 sätts tili en vektor-DNA som innehäller en uttryckningskontrollsekvens, sä att uttryck-ningskontrollsekvensen regulerar den nämnda DNA-sekven-sen.
7. Förfarande för framställning av en värdorganism som innehäller en uttryckningsvektor enligt patentkravet 9, kännetecknat därav, att en värdcell transformeras med en uttryckningsvektor som innehäller en DNA-sekvens som kodar för en förening med formeln I och är i kontroll av en uttryckningskontrollsekvens.
8. Rekombinant-DNA-sekvens, kännetecknad därav, att den * kodar för en förening med formeln I som är framställbar enligt patentkravet 1.
9. Uttryckningsvektor, kännetecknad därav, att den innehäller en DNA-sekvens, som är under kontroll av en uttryckningskontrollsekvens och kodar för en förening med ’’ formeln I som är framställbar enligt patentkravet 1.
10. Bakterie eller jäst, kännetecknad därav, att den innehäller en uttryckningsvektor enligt patentkravet 9.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH84088 | 1988-03-07 | ||
| CH84088 | 1988-03-07 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| FI891020A0 FI891020A0 (sv) | 1989-03-03 |
| FI891020A FI891020A (sv) | 1989-09-08 |
| FI96116B true FI96116B (sv) | 1996-01-31 |
| FI96116C FI96116C (sv) | 1996-05-10 |
Family
ID=4196431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI891020A FI96116C (sv) | 1988-03-07 | 1989-03-03 | Förfarande för att framställa eglinmutanter med proteasinhibitoraktivitet |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US5079229A (sv) |
| EP (1) | EP0332576B1 (sv) |
| JP (1) | JP2716191B2 (sv) |
| KR (1) | KR0134377B1 (sv) |
| AT (1) | ATE103930T1 (sv) |
| AU (1) | AU623881B2 (sv) |
| CA (1) | CA1339105C (sv) |
| DD (1) | DD283645A5 (sv) |
| DE (1) | DE58907373D1 (sv) |
| DK (1) | DK107389A (sv) |
| ES (1) | ES2063161T3 (sv) |
| FI (1) | FI96116C (sv) |
| HU (1) | HU209401B (sv) |
| IE (1) | IE62993B1 (sv) |
| IL (1) | IL89496A0 (sv) |
| NO (1) | NO178870C (sv) |
| NZ (1) | NZ228208A (sv) |
| PT (1) | PT89916B (sv) |
| TW (1) | TW211522B (sv) |
| ZA (1) | ZA891679B (sv) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6342373B1 (en) * | 1983-11-21 | 2002-01-29 | Ucp Gen-Pharma Ag | Process for preparing recombinant eglin, protease inhibitor |
| US5674833A (en) * | 1990-09-18 | 1997-10-07 | Novo Nordisk A/S | Detergent compositions containing protease and novel inhibitors for use therein |
| US5604201A (en) * | 1993-01-08 | 1997-02-18 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University, A Non-Profit Organization | Methods and reagents for inhibiting furin endoprotease |
| TW492975B (en) * | 1993-07-26 | 2002-07-01 | Novartis Ag | Tryptase inhibitor |
| JP2000506394A (ja) | 1996-09-24 | 2000-05-30 | ザ、プロクター、エンド、ギャンブル、カンパニー | 安定化されたタンパク質性プロテアーゼインヒビターおよびその変種 |
| US7001884B2 (en) * | 2001-06-18 | 2006-02-21 | Regents Of The University Of Michigan | Eglin c based drugs for treatment of disease |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
| DE3324534A1 (de) * | 1983-07-07 | 1985-01-17 | Ciba-Geigy Ag, Basel | Modifizierte protease-inhibitoren, verfahren zu ihrer herstellung und daraus bereitete pharmazeutische mittel |
| PT79519B (en) * | 1983-11-21 | 1986-12-11 | Ciba Geigy Ag | Process for preparing protease inhibitors based on egline compounds |
| US4711848A (en) * | 1984-03-14 | 1987-12-08 | Zymogenetics, Inc. | Site specific mutagenesis in alpha-1-antitrypsin |
| EP0164719B1 (en) * | 1984-06-14 | 1992-05-06 | Chiron Corporation | Active site modified protease alpha-1-antitrypsin inhibitors and their production |
| HU204563B (en) * | 1984-12-06 | 1992-01-28 | Synergen Biolog Inc | Process for producing recombinant serine-protease inhibitors and dns sequencyes for them |
| GB2188322A (en) * | 1986-03-26 | 1987-09-30 | Bayer Ag | Aprotinin and analogues thereof produced by a recombinant host |
| GB2199582A (en) * | 1987-01-07 | 1988-07-13 | Bayer Ag | Analogues of pancreatic secretory trypsin inhibitor |
-
1989
- 1989-02-28 AT AT89810152T patent/ATE103930T1/de not_active IP Right Cessation
- 1989-02-28 ES ES89810152T patent/ES2063161T3/es not_active Expired - Lifetime
- 1989-02-28 EP EP89810152A patent/EP0332576B1/de not_active Expired - Lifetime
- 1989-02-28 DE DE89810152T patent/DE58907373D1/de not_active Expired - Fee Related
- 1989-03-03 CA CA000592686A patent/CA1339105C/en not_active Expired - Fee Related
- 1989-03-03 FI FI891020A patent/FI96116C/sv not_active IP Right Cessation
- 1989-03-03 AU AU30959/89A patent/AU623881B2/en not_active Ceased
- 1989-03-03 NZ NZ228208A patent/NZ228208A/xx unknown
- 1989-03-06 DD DD89326310A patent/DD283645A5/de not_active IP Right Cessation
- 1989-03-06 IL IL89496A patent/IL89496A0/xx not_active IP Right Cessation
- 1989-03-06 ZA ZA891679A patent/ZA891679B/xx unknown
- 1989-03-06 IE IE71989A patent/IE62993B1/en not_active IP Right Cessation
- 1989-03-06 HU HU891101A patent/HU209401B/hu not_active IP Right Cessation
- 1989-03-06 NO NO890932A patent/NO178870C/no not_active IP Right Cessation
- 1989-03-06 PT PT89916A patent/PT89916B/pt not_active IP Right Cessation
- 1989-03-06 DK DK107389A patent/DK107389A/da not_active Application Discontinuation
- 1989-03-07 TW TW078101663A patent/TW211522B/zh active
- 1989-03-07 US US07/320,139 patent/US5079229A/en not_active Expired - Fee Related
- 1989-03-07 KR KR1019890002759A patent/KR0134377B1/ko not_active IP Right Cessation
- 1989-03-07 JP JP1053065A patent/JP2716191B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU623881B2 (en) | 1992-05-28 |
| IL89496A0 (en) | 1989-09-10 |
| FI891020A (sv) | 1989-09-08 |
| DE58907373D1 (de) | 1994-05-11 |
| DK107389A (da) | 1989-09-08 |
| EP0332576A2 (de) | 1989-09-13 |
| IE890719L (en) | 1989-09-07 |
| NO178870B (no) | 1996-03-11 |
| JP2716191B2 (ja) | 1998-02-18 |
| IE62993B1 (en) | 1995-03-08 |
| NO890932L (no) | 1989-09-08 |
| ATE103930T1 (de) | 1994-04-15 |
| US5079229A (en) | 1992-01-07 |
| NO178870C (no) | 1996-06-19 |
| KR0134377B1 (ko) | 1998-04-20 |
| AU3095989A (en) | 1989-09-07 |
| FI96116C (sv) | 1996-05-10 |
| EP0332576B1 (de) | 1994-04-06 |
| TW211522B (sv) | 1993-08-21 |
| KR890014735A (ko) | 1989-10-25 |
| PT89916A (pt) | 1989-11-10 |
| HU209401B (en) | 1994-05-30 |
| HUT50503A (en) | 1990-02-28 |
| DD283645A5 (de) | 1990-10-17 |
| DK107389D0 (da) | 1989-03-06 |
| FI891020A0 (sv) | 1989-03-03 |
| PT89916B (pt) | 1994-05-31 |
| ES2063161T3 (es) | 1995-01-01 |
| NO890932D0 (no) | 1989-03-06 |
| ZA891679B (en) | 1989-10-25 |
| JPH029392A (ja) | 1990-01-12 |
| EP0332576A3 (en) | 1990-09-12 |
| CA1339105C (en) | 1997-07-29 |
| NZ228208A (en) | 1990-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB | Publication of examined application | ||
| PC | Transfer of assignment of patent |
Owner name: NOVARTIS AG |
|
| MM | Patent lapsed |
Owner name: NOVARTIS AG Owner name: UCP GEN-PHARMA AG |