FI942644A0 - Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin - Google Patents

Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin

Info

Publication number
FI942644A0
FI942644A0 FI942644A FI942644A FI942644A0 FI 942644 A0 FI942644 A0 FI 942644A0 FI 942644 A FI942644 A FI 942644A FI 942644 A FI942644 A FI 942644A FI 942644 A0 FI942644 A0 FI 942644A0
Authority
FI
Finland
Prior art keywords
cellulase
yeast
procedure
humicola insolence
cloning proteins
Prior art date
Application number
FI942644A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI942644A (fi
Inventor
Henrik Dalboege
Hans Peter Heldt-Hansen
Grethe Rasmussen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI942644A publication Critical patent/FI942644A/fi
Publication of FI942644A0 publication Critical patent/FI942644A0/fi

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627Preparations containing enzymes, e.g. protease or amylase containing lipase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38654Preparations containing enzymes, e.g. protease or amylase containing oxidase or reductase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1075Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
FI942644A 1991-12-04 1994-06-03 Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin FI942644A0 (fi)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK9100379 1991-12-04
DK9100378 1991-12-04
PCT/DK1992/000360 WO1993011249A1 (fr) 1991-12-04 1992-12-02 PROCEDE DE CLONAGE DE PROTEINES DANS LA LEVURE ET CELLULASE PROVENANT DE $i(HUMICOLA INSOLENS)

Publications (2)

Publication Number Publication Date
FI942644A FI942644A (fi) 1994-06-03
FI942644A0 true FI942644A0 (fi) 1994-06-03

Family

ID=26068344

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942644A FI942644A0 (fi) 1991-12-04 1994-06-03 Menetelmä proteiinien kloonaamiseksi hiivassa ja sellulaasi, joka on Humicola insolensista peräisin

Country Status (7)

Country Link
EP (1) EP0618974A1 (fr)
JP (1) JPH08504560A (fr)
BR (1) BR9206866A (fr)
CA (1) CA2124939A1 (fr)
FI (1) FI942644A0 (fr)
MX (1) MX9206979A (fr)
WO (1) WO1993011249A1 (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69535733T2 (de) * 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
WO1996013580A1 (fr) * 1994-10-26 1996-05-09 Novo Nordisk A/S Enzyme a activite lipolytique
ATE315083T1 (de) 1995-03-17 2006-02-15 Novozymes As Neue endoglukanase
US6410701B1 (en) * 1995-05-05 2002-06-25 Human Genome Sciences, Inc. Human neuropeptide receptor
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
AU7294396A (en) * 1995-10-13 1997-04-30 Gist-Brocades B.V. Protein detection
US6184019B1 (en) 1995-10-17 2001-02-06 Röhm Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
US6723549B2 (en) 1995-10-17 2004-04-20 Ab Enzymes Oy Cellulases, the genes encoding them and uses thereof
AR005601A1 (es) * 1996-01-29 1999-06-23 Novozymes As Proceso para la remocion o blanqueo de suciedad o manchas presentes en telas celulosicas y proceso para el lavado de telas celulosicas manchadas o sucias y composicion detergente
EP0877799A1 (fr) * 1996-01-29 1998-11-18 Novo Nordisk A/S Procede de desencollage d'un tissu contenant de la cellulose
DE69738254T2 (de) 1996-05-10 2008-08-14 Novozymes A/S Methode zur bereitstellung von dna sequenzen
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
DE69738158T2 (de) 1996-07-05 2008-06-19 Novozymes A/S Alpha-Amylase Transkriptionsfaktor
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP0975745B1 (fr) * 1996-10-28 2008-02-20 Novozymes A/S Expression extracellulaire de domaines de fixation de cellulose (cbd) au moyen de bacillus
DE69716711T3 (de) 1996-12-09 2010-06-10 Novozymes A/S Verringerung von phosphorhaltigen Substanzen in Speiseölen mit hohem Anteil an nicht-hydratisierbarem Phosphor unter Verwendung einer Phospholipase, eine Phospholipase aus fädenförmigen Pilz, welche eine Phospholipase A und/oder B-Aktivität aufweist
DK1042461T3 (da) * 1997-12-22 2007-09-24 Dsm Ip Assets Bv Ekspressionskloning i filiforme svampe
WO1999045143A2 (fr) * 1998-03-06 1999-09-10 Novo Nordisk A/S Procede d'incineration de dechets et incinerateur pour la mise en oeuvre dudit procede
CN101024826B (zh) 1998-06-10 2014-09-03 诺沃奇梅兹有限公司 新的甘露聚糖酶
US6723504B1 (en) 1998-10-28 2004-04-20 Novozymes A/S Method for generating a gene library
JP2002528075A (ja) * 1998-10-28 2002-09-03 ノボザイムス アクティーゼルスカブ 遺伝子ライブラリーの生成方法
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
EP1059351A1 (fr) * 1999-06-11 2000-12-13 The Procter & Gamble Company Compositions détergentes liquides non-aqueuses contenant un composé libérant du borate et mannanase
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝
IL146935A0 (en) * 2000-04-13 2002-08-14 Emalfarb Mark Aaron High-throughput screening of expressed dna libraries in filamentous fungi
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
WO2003070957A2 (fr) * 2002-02-20 2003-08-28 Novozymes A/S Production de polypeptides de plantes
DK1713825T3 (da) * 2004-01-30 2012-11-05 Novozymes Inc Polypeptider med cellulolytisk forøgende aktivitet og polynukleotider der koder for samme
WO2006008508A1 (fr) 2004-07-16 2006-01-26 Danisco A/S Procede de demucilagination enzymatique
BRPI0708949B1 (pt) 2006-03-20 2017-04-04 Novozymes As métodos para degradar ou converter um material celulósico, para produzir uma susbtância, e para produzir um polipeptídeo tendo atividade endoglucanase, e, célula microbiana recombinante
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
WO2009033071A2 (fr) 2007-09-07 2009-03-12 Dyadic International, Inc. Enzymes fongiques inédites

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK187280A (da) * 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
WO1989009259A1 (fr) * 1988-03-24 1989-10-05 Novo-Nordisk A/S Preparation de cellulase
AU639570B2 (en) * 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
ES2080957T3 (es) * 1990-07-13 1996-02-16 Transkaryotic Therapies Inc Metodo para escrutar una genoteca.

Also Published As

Publication number Publication date
BR9206866A (pt) 1995-11-21
FI942644A (fi) 1994-06-03
JPH08504560A (ja) 1996-05-21
WO1993011249A1 (fr) 1993-06-10
EP0618974A1 (fr) 1994-10-12
CA2124939A1 (fr) 1993-06-10
MX9206979A (es) 1993-07-01

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