FI92884B - Förfarande för mätning av den fria fraktionen av liganden i biologiska vätskor - Google Patents
Förfarande för mätning av den fria fraktionen av liganden i biologiska vätskor Download PDFInfo
- Publication number
- FI92884B FI92884B FI903348A FI903348A FI92884B FI 92884 B FI92884 B FI 92884B FI 903348 A FI903348 A FI 903348A FI 903348 A FI903348 A FI 903348A FI 92884 B FI92884 B FI 92884B
- Authority
- FI
- Finland
- Prior art keywords
- ligand
- antibody
- free
- binding
- analog
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57415—Specifically defined cancers of breast
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Endocrinology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Claims (10)
1. Förfarande för bestämning av fria delen av en ligand i ett biologiskt vätskeprov, vilket även innehäller 5 en del av liganden bunden vid ett eller flera naturliga bindemedel, genom utnyttjande av en signalreagens, vilken bestär av en antikropp för liganden, och av en differen-tiellt bindande ligandanalog, vilken tävlar med liganden för att binda sig vid antikroppen, varvid förfarandet om-10 fattar inkubering av provet med analogen och antikroppen och observerande av i hur hög grad antikroppen bundit sig vid analogen, kännetecknat därav, att antikroppen är en monoklonal antikropp gentemot liganden och att analogen väls sä att den har lägre affinitet än ligan-15 den för att binda sig vid antikroppen.
2. Förfarande enligt patentkrav 1, kanne-t e c k n a d därav, att bindningsaffiniteten hos analogen i förhällande tili antikroppen är 0,01 - 10 % av bindningsaffiniteten hos den fria liganden i förhällande 20 tili antikroppen.
3. Förfarande enligt patentkrav 1 eller 2, kännetecknad därav, att den differentiellt bindande ligandanalogen insolubiliseras före inkubationen.
. 4. Förfarande enligt patentkrav 3, k ä n n e - 25 tecknad därav, att den differentiellt bindande ligandanalogen insolubiliseras genom att den kovalent binds vid cellulosa- eller polystyrenpartiklar.
5. Förfarande enligt nägot av patentkraven 1-4, kännetecknad därav, att antikroppen märks med 30 1-125.
6. Förfarande enligt nägot av patentkraven 1-5, kännetecknad därav, att förhällandet a) : b) ligger mellan 0,1 och 10, varvid a) är (antikroppens af-finitetskonstant för analogen gänger effektiva koncentra- 35 tionen hos analogen), och b) är (antikroppens affinitets- so ^2884 konstant för liganden gänger koncentrationen hos liganden som föreligger bunden vid antikroppen vid slutet av inku-bationen).
7. Förfarande enligt nägot av patentkraven 1-6, 5 kännetecknad därav, att förhällandet effektiv analogkoncentration tili antikroppkoncentrationen uppgär tili f rän 10 till 105.
8. Förfarande enligt nägot av patentkraven 1-7, kännetecknad därav, att koncentrationen av 10 analogbindningsställen för antikroppen och den fria li-gandkoncentrationen är väsentligen lika vid början och slutet av inkubationen.
9. Förfarande enligt nägot av patentkraven 1-8, kännetecknad därav, att liganden är ett 15 tyroidhormon, en steroid eller kortisol.
10. Förfarande enligt patentkrav 9, kännetecknad därav att liganden är tyroxin och att den differentiellt bindande ligandanalogen är tri-jodotyronin. Il
Applications Claiming Priority (12)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB888800419A GB8800419D0 (en) | 1988-01-08 | 1988-01-08 | Method for measuring free fraction of ligands in biological fluids |
| GB8800419 | 1988-01-08 | ||
| PCT/GB1989/000003 WO1989006363A1 (en) | 1988-01-08 | 1989-01-05 | A method for measuring the free fraction of ligands in biological fluids |
| GB8900003 | 1989-01-05 | ||
| US47396490A | 1990-04-17 | 1990-04-17 | |
| US47396490 | 1990-04-17 | ||
| US55158090A | 1990-07-02 | 1990-07-02 | |
| US55158090 | 1990-07-02 | ||
| US88507092A | 1992-05-18 | 1992-05-18 | |
| US88507092 | 1992-05-18 | ||
| US08/022,416 US5278080A (en) | 1988-01-08 | 1993-02-11 | Method for measuring the free fraction of ligands in biological fluids |
| US2241693 | 1993-02-11 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI903348A0 FI903348A0 (sv) | 1990-07-03 |
| FI92884B true FI92884B (sv) | 1994-09-30 |
| FI92884C FI92884C (sv) | 1995-01-10 |
Family
ID=27516786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI903348A FI92884C (sv) | 1988-01-08 | 1990-07-03 | Förfarande för mätning av den fria fraktionen av liganden i biologiska vätskor |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5278080A (sv) |
| EP (1) | EP0324540B2 (sv) |
| JP (1) | JP2718229B2 (sv) |
| AT (1) | ATE64469T1 (sv) |
| AU (1) | AU619712B2 (sv) |
| CA (1) | CA1340536C (sv) |
| DE (1) | DE68900106D1 (sv) |
| ES (1) | ES2029114T5 (sv) |
| FI (1) | FI92884C (sv) |
| GB (1) | GB8800419D0 (sv) |
| WO (1) | WO1989006363A1 (sv) |
| ZA (1) | ZA8918B (sv) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3626468A1 (de) * | 1986-08-05 | 1988-02-11 | Hoechst Ag | Verfahren und testkit zur bestimmung freier wirkstoffe in biologischen fluessigkeiten |
| DE3727238A1 (de) * | 1987-08-14 | 1989-02-23 | Henning Berlin Gmbh | Immunologisches bestimmungsverfahren zur bestimmung von hapteneigenschaften aufweisenden freien substanzen |
| US5364796A (en) * | 1989-07-11 | 1994-11-15 | Pb Diagnostics Systems, Inc. | Diagnostic assay system |
| IT1254858B (it) * | 1992-04-14 | 1995-10-11 | Metodo per la determinazione della frazione libera di sostanze presenti nei fluidi biologici. | |
| DE4214922C2 (de) * | 1992-05-06 | 1996-06-13 | Brahms Diagnostica Gmbh | Verfahren zur quantitativen Bestimmung des Anteils der freien Form eines Schilddrüsenhormon-Liganden in einer biologischen Flüssigkeit und Kit zur Durchführung eines solchen Verfahrens |
| EP0606950A3 (en) * | 1993-01-13 | 1995-07-12 | Eastman Kodak Co | Method for determining the reaction rate of analytes. |
| DE69530315T2 (de) * | 1994-11-24 | 2004-02-12 | Inverness Medical Switzerland Gmbh | Rückgewinnung und verwendungen von spezifischen bindungsreagenzen |
| DE19504198A1 (de) * | 1995-02-09 | 1996-08-14 | Behringwerke Ag | Kompetitiver Immuntest unter Verwendung komplexierter Analytderivate |
| US6143578A (en) * | 1996-05-10 | 2000-11-07 | Bayer Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
| US5888835A (en) * | 1996-05-10 | 1999-03-30 | Chiron Diagnostics Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
| EP0880704B1 (en) * | 1996-07-18 | 2003-09-24 | Dade Behring Marburg GmbH | Reagents for assays for mycophenolic acid |
| US6087088A (en) * | 1997-01-31 | 2000-07-11 | Bayer Corporation | Binding assays using more than one label for determining analyte in the presence of interfering factors |
| WO2010013525A1 (ja) * | 2008-07-31 | 2010-02-04 | 日東紡績株式会社 | 複合体の測定方法およびそれに用いるキット |
| JP5997446B2 (ja) * | 2011-02-03 | 2016-09-28 | アークレイ株式会社 | 甲状腺ホルモン固定化担体の液体試薬およびその用途 |
| CN114544831B (zh) * | 2022-02-21 | 2024-05-10 | 成都翼泰生物科技有限公司 | 分离血液中游离甲状腺激素的方法及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4292296A (en) * | 1978-09-12 | 1981-09-29 | Baxter Travenol Laboratories, Inc. | Diagnostic method |
| US4366143A (en) * | 1979-09-24 | 1982-12-28 | Amersham International Public Limited Company | Assay for the free portion of substances in biological fluids |
| WO1983003306A1 (en) * | 1982-03-19 | 1983-09-29 | Roger Philip Ekins | Method and composition for free ligand assays |
| EP0089806B1 (en) * | 1982-03-22 | 1989-06-28 | AMERSHAM INTERNATIONAL plc | Assay for the free portion of substances in biological fluids |
| ZA837119B (en) * | 1982-10-07 | 1984-12-24 | Amersham Int Plc | Assay for the free portion of substances in biological fluids |
| GB2134806B (en) | 1983-02-07 | 1986-07-16 | English Clays Lovering Pochin | A tube pressure filter |
| GB8408193D0 (en) * | 1984-03-30 | 1984-05-10 | Cambridge Patent Dev | Antibodies |
| DE3727238A1 (de) * | 1987-08-14 | 1989-02-23 | Henning Berlin Gmbh | Immunologisches bestimmungsverfahren zur bestimmung von hapteneigenschaften aufweisenden freien substanzen |
-
1988
- 1988-01-08 GB GB888800419A patent/GB8800419D0/en active Pending
-
1989
- 1989-01-03 ZA ZA8918A patent/ZA8918B/xx unknown
- 1989-01-05 DE DE8989300062T patent/DE68900106D1/de not_active Expired - Lifetime
- 1989-01-05 WO PCT/GB1989/000003 patent/WO1989006363A1/en active IP Right Grant
- 1989-01-05 CA CA000587598A patent/CA1340536C/en not_active Expired - Lifetime
- 1989-01-05 EP EP89300062A patent/EP0324540B2/en not_active Expired - Lifetime
- 1989-01-05 JP JP1501274A patent/JP2718229B2/ja not_active Expired - Lifetime
- 1989-01-05 AT AT89300062T patent/ATE64469T1/de not_active IP Right Cessation
- 1989-01-05 AU AU29251/89A patent/AU619712B2/en not_active Expired
- 1989-01-05 ES ES89300062T patent/ES2029114T5/es not_active Expired - Lifetime
-
1990
- 1990-07-03 FI FI903348A patent/FI92884C/sv not_active IP Right Cessation
-
1993
- 1993-02-11 US US08/022,416 patent/US5278080A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| GB8800419D0 (en) | 1988-02-10 |
| ES2029114T3 (es) | 1992-07-16 |
| US5278080A (en) | 1994-01-11 |
| EP0324540B2 (en) | 2000-12-27 |
| FI903348A0 (sv) | 1990-07-03 |
| JPH03502243A (ja) | 1991-05-23 |
| EP0324540A1 (en) | 1989-07-19 |
| AU619712B2 (en) | 1992-01-30 |
| CA1340536C (en) | 1999-05-11 |
| WO1989006363A1 (en) | 1989-07-13 |
| ES2029114T5 (es) | 2001-04-16 |
| ZA8918B (en) | 1989-10-25 |
| AU2925189A (en) | 1989-08-01 |
| FI92884C (sv) | 1995-01-10 |
| EP0324540B1 (en) | 1991-06-12 |
| JP2718229B2 (ja) | 1998-02-25 |
| ATE64469T1 (de) | 1991-06-15 |
| DE68900106D1 (de) | 1991-07-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB | Publication of examined application | ||
| FG | Patent granted |
Owner name: AMERLITE DIAGNOSTICS LIMITED |
|
| MA | Patent expired |