ES2029114T3 - Un metodo para medir la fraccion libre de ligandos en fluidos biologicos. - Google Patents
Un metodo para medir la fraccion libre de ligandos en fluidos biologicos.Info
- Publication number
- ES2029114T3 ES2029114T3 ES198989300062T ES89300062T ES2029114T3 ES 2029114 T3 ES2029114 T3 ES 2029114T3 ES 198989300062 T ES198989300062 T ES 198989300062T ES 89300062 T ES89300062 T ES 89300062T ES 2029114 T3 ES2029114 T3 ES 2029114T3
- Authority
- ES
- Spain
- Prior art keywords
- ligand
- antibody
- ligands
- measuring
- analogue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57415—Specifically defined cancers of breast
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Endocrinology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
UN ENSAYO EN UNA ETAPA DE LA PARTE LIBRE DE UN LIGANDO EN UNA MUESTRA BIOLOGICA INCLUYE LA INCUBACION DE UNA MEZCLA DE LA MUESTRA CON UN ANTICUERPO MARCADO PARA EL LIGANDO Y UN ANALOGO DE LIGANDO QUE COMPITE CON EL LIGANDO PARA ENLAZAR EL ANTICUERPO. EL ENSAYO SE CARACTERIZA POR USAR UN ANTICUERPO MONOCLONAL Y POR ELEGIR UN ANALOGO DE LIGANDO QUE TIENE UNA AFINIDAD POR EL ANTICUERPO INFERIOR A LA DEL LIGANDO. UN ANALOGO DEL LIGANDO INSOLUBILIZADO PREFERIBLEMENTE TIENE UNA AFINIDAD LIGANTE POR EL ANTICUERPO DE 0,01-10 % DE LA DEL LIGANDO. EJEMPLOS DE PARES LIGANDOÑANALOGO DE LIGANDO SON T4/T3 (TIROXINA/TRIYODOTIRONINA); TESTOSTERONA/ETIOCOLANOL; Y T3/T2
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB888800419A GB8800419D0 (en) | 1988-01-08 | 1988-01-08 | Method for measuring free fraction of ligands in biological fluids |
US47396490A | 1990-04-17 | 1990-04-17 | |
US55158090A | 1990-07-02 | 1990-07-02 | |
US88507092A | 1992-05-18 | 1992-05-18 | |
US08/022,416 US5278080A (en) | 1988-01-08 | 1993-02-11 | Method for measuring the free fraction of ligands in biological fluids |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2029114T3 true ES2029114T3 (es) | 1992-07-16 |
ES2029114T5 ES2029114T5 (es) | 2001-04-16 |
Family
ID=27516786
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89300062T Expired - Lifetime ES2029114T5 (es) | 1988-01-08 | 1989-01-05 | Un metodo para medir la fraccion libre de ligandos en fluidos biologicos. |
Country Status (12)
Country | Link |
---|---|
US (1) | US5278080A (es) |
EP (1) | EP0324540B2 (es) |
JP (1) | JP2718229B2 (es) |
AT (1) | ATE64469T1 (es) |
AU (1) | AU619712B2 (es) |
CA (1) | CA1340536C (es) |
DE (1) | DE68900106D1 (es) |
ES (1) | ES2029114T5 (es) |
FI (1) | FI92884C (es) |
GB (1) | GB8800419D0 (es) |
WO (1) | WO1989006363A1 (es) |
ZA (1) | ZA8918B (es) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3626468A1 (de) * | 1986-08-05 | 1988-02-11 | Hoechst Ag | Verfahren und testkit zur bestimmung freier wirkstoffe in biologischen fluessigkeiten |
DE3727238A1 (de) * | 1987-08-14 | 1989-02-23 | Henning Berlin Gmbh | Immunologisches bestimmungsverfahren zur bestimmung von hapteneigenschaften aufweisenden freien substanzen |
US5364796A (en) * | 1989-07-11 | 1994-11-15 | Pb Diagnostics Systems, Inc. | Diagnostic assay system |
IT1254858B (it) * | 1992-04-14 | 1995-10-11 | Metodo per la determinazione della frazione libera di sostanze presenti nei fluidi biologici. | |
DE4214922C2 (de) * | 1992-05-06 | 1996-06-13 | Brahms Diagnostica Gmbh | Verfahren zur quantitativen Bestimmung des Anteils der freien Form eines Schilddrüsenhormon-Liganden in einer biologischen Flüssigkeit und Kit zur Durchführung eines solchen Verfahrens |
EP0606950A3 (en) * | 1993-01-13 | 1995-07-12 | Eastman Kodak Co | Method for determining the reaction rate of analytes. |
ATE237137T1 (de) * | 1994-11-24 | 2003-04-15 | Inverness Medical Switzerland | Rückgewinnung und verwendungen von spezifischen bindungsreagenzen |
DE19504198A1 (de) * | 1995-02-09 | 1996-08-14 | Behringwerke Ag | Kompetitiver Immuntest unter Verwendung komplexierter Analytderivate |
US5888835A (en) * | 1996-05-10 | 1999-03-30 | Chiron Diagnostics Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
US6143578A (en) * | 1996-05-10 | 2000-11-07 | Bayer Corporation | Method and apparatus for wash, resuspension, recollection and localization of magnetizable particles in assays using magnetic separation technology |
DE69725118T2 (de) * | 1996-07-18 | 2004-07-08 | Dade Behring Marburg Gmbh | Reagentien für tests für mycophenolsäure |
US6087088A (en) * | 1997-01-31 | 2000-07-11 | Bayer Corporation | Binding assays using more than one label for determining analyte in the presence of interfering factors |
US8900882B2 (en) * | 2008-07-31 | 2014-12-02 | Nitto Boseki Co., Ltd. | Method of assaying complex and kit to be used therefor |
JP5997446B2 (ja) | 2011-02-03 | 2016-09-28 | アークレイ株式会社 | 甲状腺ホルモン固定化担体の液体試薬およびその用途 |
CN114544831B (zh) * | 2022-02-21 | 2024-05-10 | 成都翼泰生物科技有限公司 | 分离血液中游离甲状腺激素的方法及其应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4292296A (en) * | 1978-09-12 | 1981-09-29 | Baxter Travenol Laboratories, Inc. | Diagnostic method |
US4366143A (en) * | 1979-09-24 | 1982-12-28 | Amersham International Public Limited Company | Assay for the free portion of substances in biological fluids |
DE3382044D1 (de) * | 1982-03-19 | 1991-01-17 | Ekins | Verfahren zur pruefung von freien liganden. |
EP0089806B1 (en) * | 1982-03-22 | 1989-06-28 | AMERSHAM INTERNATIONAL plc | Assay for the free portion of substances in biological fluids |
ZA837119B (en) * | 1982-10-07 | 1984-12-24 | Amersham Int Plc | Assay for the free portion of substances in biological fluids |
GB2134806B (en) | 1983-02-07 | 1986-07-16 | English Clays Lovering Pochin | A tube pressure filter |
GB8408193D0 (en) * | 1984-03-30 | 1984-05-10 | Cambridge Patent Dev | Antibodies |
DE3727238A1 (de) * | 1987-08-14 | 1989-02-23 | Henning Berlin Gmbh | Immunologisches bestimmungsverfahren zur bestimmung von hapteneigenschaften aufweisenden freien substanzen |
-
1988
- 1988-01-08 GB GB888800419A patent/GB8800419D0/en active Pending
-
1989
- 1989-01-03 ZA ZA8918A patent/ZA8918B/xx unknown
- 1989-01-05 ES ES89300062T patent/ES2029114T5/es not_active Expired - Lifetime
- 1989-01-05 DE DE8989300062T patent/DE68900106D1/de not_active Expired - Lifetime
- 1989-01-05 CA CA000587598A patent/CA1340536C/en not_active Expired - Lifetime
- 1989-01-05 AT AT89300062T patent/ATE64469T1/de not_active IP Right Cessation
- 1989-01-05 WO PCT/GB1989/000003 patent/WO1989006363A1/en active IP Right Grant
- 1989-01-05 JP JP1501274A patent/JP2718229B2/ja not_active Expired - Lifetime
- 1989-01-05 AU AU29251/89A patent/AU619712B2/en not_active Expired
- 1989-01-05 EP EP89300062A patent/EP0324540B2/en not_active Expired - Lifetime
-
1990
- 1990-07-03 FI FI903348A patent/FI92884C/fi not_active IP Right Cessation
-
1993
- 1993-02-11 US US08/022,416 patent/US5278080A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0324540B1 (en) | 1991-06-12 |
AU619712B2 (en) | 1992-01-30 |
FI92884B (fi) | 1994-09-30 |
JP2718229B2 (ja) | 1998-02-25 |
AU2925189A (en) | 1989-08-01 |
FI92884C (fi) | 1995-01-10 |
CA1340536C (en) | 1999-05-11 |
EP0324540B2 (en) | 2000-12-27 |
DE68900106D1 (de) | 1991-07-18 |
FI903348A0 (fi) | 1990-07-03 |
GB8800419D0 (en) | 1988-02-10 |
US5278080A (en) | 1994-01-11 |
JPH03502243A (ja) | 1991-05-23 |
EP0324540A1 (en) | 1989-07-19 |
ZA8918B (en) | 1989-10-25 |
WO1989006363A1 (en) | 1989-07-13 |
ATE64469T1 (de) | 1991-06-15 |
ES2029114T5 (es) | 2001-04-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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