HUT53230A - Method for radioimmunological detecting prostaglandin e down 2 content - Google Patents

Method for radioimmunological detecting prostaglandin e down 2 content

Info

Publication number
HUT53230A
HUT53230A HU49789A HU49789A HUT53230A HU T53230 A HUT53230 A HU T53230A HU 49789 A HU49789 A HU 49789A HU 49789 A HU49789 A HU 49789A HU T53230 A HUT53230 A HU T53230A
Authority
HU
Hungary
Prior art keywords
reaction
prostaglandin
incubation
antibody
equilibrium
Prior art date
Application number
HU49789A
Other languages
Hungarian (hu)
Other versions
HU202323B (en
Inventor
Jolan Fazekas
Dinyak Katalin Makraine
Istvan Mucha
Original Assignee
Mta Izotop Intezete
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mta Izotop Intezete filed Critical Mta Izotop Intezete
Priority to HU49789A priority Critical patent/HU202323B/en
Publication of HUT53230A publication Critical patent/HUT53230A/en
Publication of HU202323B publication Critical patent/HU202323B/en

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  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Antigen-antibody assaying of prostaglandin E2 contg. - bio-samples is improved, when a sequential satn. reaction-based on mono- 125-tyrosine-methyl-ester-deriv. of prostaglandin E2 as isotopic marking indicator and on a specific PGE2 antibody as protein-fixative-replaces a previously used method of a satn. reaction in a state of equilibrium. New equilibrium conditions are maintained for the incubation of inactive antigen and antibodies, but the incubation of antibodies and radioligands occurs under. non-equalibrium conditions. This movel method increases the sensitivity of the data three-four fold compared to equilibrium state radio-immunological assaying. - Reaction parameters are optimised in incubation processes i.e. reaction periods, temps. and antibody concns. - Reagents, are identical to those used in previous detections.
HU49789A 1989-02-01 1989-02-01 Method for radioimmunological determination of prostaglandine e2 content HU202323B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
HU49789A HU202323B (en) 1989-02-01 1989-02-01 Method for radioimmunological determination of prostaglandine e2 content

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
HU49789A HU202323B (en) 1989-02-01 1989-02-01 Method for radioimmunological determination of prostaglandine e2 content

Publications (2)

Publication Number Publication Date
HUT53230A true HUT53230A (en) 1990-09-28
HU202323B HU202323B (en) 1991-02-28

Family

ID=10949853

Family Applications (1)

Application Number Title Priority Date Filing Date
HU49789A HU202323B (en) 1989-02-01 1989-02-01 Method for radioimmunological determination of prostaglandine e2 content

Country Status (1)

Country Link
HU (1) HU202323B (en)

Also Published As

Publication number Publication date
HU202323B (en) 1991-02-28

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Legal Events

Date Code Title Description
HMM4 Cancellation of final prot. due to non-payment of fee
HMM4 Cancellation of final prot. due to non-payment of fee
HNF4 Restoration of lapsed final prot.