HUT53230A - Method for radioimmunological detecting prostaglandin e down 2 content - Google Patents
Method for radioimmunological detecting prostaglandin e down 2 contentInfo
- Publication number
- HUT53230A HUT53230A HU49789A HU49789A HUT53230A HU T53230 A HUT53230 A HU T53230A HU 49789 A HU49789 A HU 49789A HU 49789 A HU49789 A HU 49789A HU T53230 A HUT53230 A HU T53230A
- Authority
- HU
- Hungary
- Prior art keywords
- reaction
- prostaglandin
- incubation
- antibody
- equilibrium
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Antigen-antibody assaying of prostaglandin E2 contg. - bio-samples is improved, when a sequential satn. reaction-based on mono- 125-tyrosine-methyl-ester-deriv. of prostaglandin E2 as isotopic marking indicator and on a specific PGE2 antibody as protein-fixative-replaces a previously used method of a satn. reaction in a state of equilibrium. New equilibrium conditions are maintained for the incubation of inactive antigen and antibodies, but the incubation of antibodies and radioligands occurs under. non-equalibrium conditions. This movel method increases the sensitivity of the data three-four fold compared to equilibrium state radio-immunological assaying. - Reaction parameters are optimised in incubation processes i.e. reaction periods, temps. and antibody concns. - Reagents, are identical to those used in previous detections.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU49789A HU202323B (en) | 1989-02-01 | 1989-02-01 | Method for radioimmunological determination of prostaglandine e2 content |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU49789A HU202323B (en) | 1989-02-01 | 1989-02-01 | Method for radioimmunological determination of prostaglandine e2 content |
Publications (2)
Publication Number | Publication Date |
---|---|
HUT53230A true HUT53230A (en) | 1990-09-28 |
HU202323B HU202323B (en) | 1991-02-28 |
Family
ID=10949853
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU49789A HU202323B (en) | 1989-02-01 | 1989-02-01 | Method for radioimmunological determination of prostaglandine e2 content |
Country Status (1)
Country | Link |
---|---|
HU (1) | HU202323B (en) |
-
1989
- 1989-02-01 HU HU49789A patent/HU202323B/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
HU202323B (en) | 1991-02-28 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
HMM4 | Cancellation of final prot. due to non-payment of fee | ||
HMM4 | Cancellation of final prot. due to non-payment of fee | ||
HNF4 | Restoration of lapsed final prot. |