FI92221B - Amperometrisk metod för kvantitativ bestämning av 1,4-dihydronikotinamidadenindinukleotid (NADH) i lösning - Google Patents
Amperometrisk metod för kvantitativ bestämning av 1,4-dihydronikotinamidadenindinukleotid (NADH) i lösning Download PDFInfo
- Publication number
- FI92221B FI92221B FI886050A FI886050A FI92221B FI 92221 B FI92221 B FI 92221B FI 886050 A FI886050 A FI 886050A FI 886050 A FI886050 A FI 886050A FI 92221 B FI92221 B FI 92221B
- Authority
- FI
- Finland
- Prior art keywords
- nadh
- electrode
- enzyme
- resin
- carbon
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/40—Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/817—Enzyme or microbe electrode
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Sustainable Development (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Electrodes For Compound Or Non-Metal Manufacture (AREA)
Claims (10)
1. Förfarande för kvantitativ bestämning av 1,4-dihyd-ronikotinamidadenindinukleotid (NADH) i lösning, i vilket förfarande den NADH-haltiga lösningen bringas i kontakt med en aktivkolelektrod och aktivkolelektroden hälles under en kontrollerad, fast potential vilken kan ästad-komma oxidation av NADH pä elektrodens yta, och kolelekt-rodens utgängsström mäts, kännetecknat därav, att man i förfarandet använder en ädelmetallhaltig aktivkolelektrod, vilken omfattar ett poröst, heterogent, hartsbundet skikt av aktivkol- eller -grafitpartiklar vilka innehäller den finfördelade ädelmetallen eller motsvarande oxider förabsorberade därpä, och är bundna med ett naturligt eller syntetiskt hartsbindemedel.
2. Förfarande enligt patentkravet 1, kännetecknat därav, att hartsbindemedlet är ett kolfluorharts, företrädesvis polytetrafluoretylen.
3. Förfarande enligt patentkravet 1 eller 2, kännetecknat därav, att de hartsbundna ädelmetallhalti-ga koi- eller grafitpartiklarna är bildade tili ett hartsbundet ytskikt pä ett underliggande elledande stöd-organ.
4. Förfarande enligt patentkravet 3, kännetecknat därav, att det elledande stödorganet är ett elledande kolpappersskikt, i vilket de ädelmetallhaltiga kol-eller grafitpartiklarna är bundna som ett ytskikt.
5. Förfarande enligt nägot av patentkraven 1-4, kännetecknat därav, att koi- eller grafitpartiklarna är förplatinerade eller förpalladiserade partiklar, vilkas partikelstorlek är inom omrädet 5-30 nm och vilka innehäller finfördelat, kolloidiskt platina eller palladium förabsorberat pä grafit- eller kolpartiklarna, var- 20 92221 vid partikelstorleken av de kolloidiska platina- eller palladiumpartiklarna är mellan 1,5-2,5 nm.
6. Förfarande enligt nägot av patentkraven 1-5, k ä n -netecknat därav, att det används för övervakning av produktionen eller förbrukningen av NADH mellan enzy-met och dess substrat i en enzymatisk reaktion, där an-tingen NADH eller NAD är närvarande som kofaktor och där man använder den ädelmetallhaltiga kolelektroden, pä eller i vilken enzymet har tillsatts eller immobiliserats, och där elektroden används för uppföljning av enzymets aktivitet genom förändring av NADH-koncentrationen, när elektroden är i kontakt med ett prov, som innehäller en-zymsubstrat, och i närvaro av antingen NAD eller NADH som kofaktor, vilken hör tili reaktionen mellan enzymet och dess substrat.
7. Förfarande enligt patentkravet 6, känneteck-n a t därav, att man använder däri en ädelmetallhaltig elektrod, pä eller i vilken enzymet har tillsatts eller immobiliserats tillsammans med kofaktorn NADH eller NAD, beroende pä fallet, varvid kofaktorn NADH eller NAD pä detta sätt tillförs provet via elektroden.
8. Engängsenzymelektrod avsedd för användning i förfarande enligt patentkravet 7, kännetecknat därav, att den bildas av en aktivkolelektrod, som omfattar eller innehäller ett poröst, hartsbundet, heterogent skikt av aktivkol- eller -grafitpartiklar, vilka innehäller den finfördelade ädelmetallen eller motsvarande oxid förabsorberad därpä, och partiklarna är sinsemellan bund-na med ett syntetiskt hydrofobt hartsbindemedel, varvid enzymet har immobiliserats i eller pä det hartsbundna skiktet tillsammans med NAD eller NADH som kofaktor av enzymet, varvid elektroden svarar amperometriskt mot enzymets aktivitet i närvaro av sitt substrat och NAD eller NADH, beroende pä fallet. 21 92221
9. Engängsenzymelektrod enligt patentkravet 8, k ä n -netecknat därav, att aktivkolelektroden omfattar poröst, hartsbundet, heterogent ytskikt raed förplatinera-de eller förpalladiserade aktivkol- eller -grafitparti-klar, vilka är bundna med ett koifluorharts, och vilket är uppburet av ett underliggande stödorgan och i eller pä vilket enzymet eller kofaktoren har inunobiliserats.
10. Engängsenzymelektrod enligt patentkravet 9, kännetecknat därav, att det stödorgan, som uppbär de förplatinerade eller förpalladiserade aktivkol-eller -grafitpartiklarna, är ett elledande kolpapper.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB878710472A GB8710472D0 (en) | 1987-05-01 | 1987-05-01 | Amperometric method |
| GB8710472 | 1987-05-01 | ||
| GB8800338 | 1988-04-29 | ||
| PCT/GB1988/000338 WO1988008447A1 (en) | 1987-05-01 | 1988-04-29 | Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (nadh) in solution |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI886050A FI886050A (sv) | 1988-12-30 |
| FI92221B true FI92221B (sv) | 1994-06-30 |
| FI92221C FI92221C (sv) | 1994-10-10 |
Family
ID=10616757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI886050A FI92221C (sv) | 1987-05-01 | 1988-12-30 | Amperometrisk metod för kvantitativ bestämning av 1,4-dihydronikotinamidadenindinukleotid (NADH) i lösning |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US5122456A (sv) |
| EP (2) | EP0289344B1 (sv) |
| JP (2) | JP2528956B2 (sv) |
| KR (1) | KR960001501B1 (sv) |
| AU (2) | AU612366B2 (sv) |
| CA (1) | CA1304127C (sv) |
| DE (1) | DE3882078T2 (sv) |
| DK (1) | DK169559B1 (sv) |
| ES (1) | ES2058271T3 (sv) |
| FI (1) | FI92221C (sv) |
| GB (3) | GB8710472D0 (sv) |
| HU (1) | HU205625B (sv) |
| IE (1) | IE60238B1 (sv) |
| IL (2) | IL86223A (sv) |
| MX (1) | MX167769B (sv) |
| NO (1) | NO179953C (sv) |
| RU (1) | RU1806187C (sv) |
| WO (2) | WO1988008446A1 (sv) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8710472D0 (en) * | 1987-05-01 | 1987-06-03 | Cambridge Life Sciences | Amperometric method |
| GB8817997D0 (en) * | 1988-07-28 | 1988-09-01 | Cambridge Life Sciences | Enzyme electrodes & improvements in manufacture thereof |
| DE3932247A1 (de) * | 1989-09-27 | 1991-04-04 | Biotechnolog Forschung Gmbh | Elektrodenmaterial, elektroden, verfahren zur herstellung und verwendung der elektrode |
| TW279133B (sv) * | 1990-12-13 | 1996-06-21 | Elan Med Tech | |
| AT397513B (de) * | 1992-12-15 | 1994-04-25 | Avl Verbrennungskraft Messtech | Amperometrische enzymelektrode |
| US5429735A (en) * | 1994-06-27 | 1995-07-04 | Miles Inc. | Method of making and amperometric electrodes |
| IE72524B1 (en) * | 1994-11-04 | 1997-04-23 | Elan Med Tech | Analyte-controlled liquid delivery device and analyte monitor |
| DE4442253A1 (de) * | 1994-11-28 | 1996-05-30 | Bayer Corp N D Ges D Staates I | Elektrochemischer Enzymbiosensor |
| US6413410B1 (en) | 1996-06-19 | 2002-07-02 | Lifescan, Inc. | Electrochemical cell |
| FR2743575B1 (fr) * | 1996-01-17 | 1998-03-13 | Univ Toulouse | Procede perfectionne de modification en surface d'une electrode pour la realisation d'un reaction electrochimique et electrodes obtenues |
| ATE275723T1 (de) | 1996-11-07 | 2004-09-15 | Cambridge Sensors Ltd | Elektroden und ihre verwendung in assays |
| CA2294610A1 (en) | 1997-06-16 | 1998-12-23 | George Moshe Katz | Methods of calibrating and testing a sensor for in vivo measurement of an analyte and devices for use in such methods |
| KR100491316B1 (ko) * | 2002-05-29 | 2005-05-24 | 삼성엔지니어링 주식회사 | 망간이 고정된 흑연 또는 뉴트랄 레드가 결합된 흑연을포함하는 ｎａｄ(ｐ)+ 또는 ｎａｄ(ｐ)ｈ의 직접 환원또는 산화용 전극 |
| RU2476869C2 (ru) * | 2007-09-18 | 2013-02-27 | Алтизайм Интернэшнл Лтд | Ферментный электрод |
| JP5362406B2 (ja) * | 2009-03-25 | 2013-12-11 | 三洋電機株式会社 | 燃料電池 |
| JP5930810B2 (ja) | 2011-04-26 | 2016-06-08 | アークレイ株式会社 | 分析用具 |
| EP2731169B1 (en) * | 2011-07-07 | 2017-01-04 | Toyota Jidosha Kabushiki Kaisha | Secondary battery |
| WO2020148414A1 (en) | 2019-01-18 | 2020-07-23 | Frieslandcampina Nederland B.V. | Shaped cheese product |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4044193A (en) * | 1971-06-16 | 1977-08-23 | Prototech Company | Finely particulated colloidal platinum compound and sol for producing the same, and method of preparation of fuel cell electrodes and the like employing the same |
| SU593439A1 (ru) * | 1975-08-04 | 1980-10-07 | Ордена Трудового Красного Знамени Институт Химических Наук Ан Казахской Сср | Способ получени электропроводных фермент-ковакторных систем |
| US4166143A (en) * | 1977-01-24 | 1979-08-28 | Prototech Company | Control of the interaction of novel platinum-on-carbon electrocatalysts with fluorinated hydrocarbon resins in the preparation of fuel cell electrodes |
| US4321123A (en) * | 1978-04-21 | 1982-03-23 | Matsushita Electric Industrial Co., Ltd. | Coenzyme immobilized electrode |
| JPS5816695B2 (ja) * | 1978-04-24 | 1983-04-01 | 松下電器産業株式会社 | 酵素電極 |
| JPS584982B2 (ja) * | 1978-10-31 | 1983-01-28 | 松下電器産業株式会社 | 酵素電極 |
| US4293396A (en) * | 1979-09-27 | 1981-10-06 | Prototech Company | Thin carbon-cloth-based electrocatalytic gas diffusion electrodes, and electrochemical cells comprising the same |
| US4478696A (en) * | 1982-07-21 | 1984-10-23 | Prototech Company | Ionizable reducing and oxidizing gaseous supply means and process for catalytic barriers and electrodes |
| GB8606824D0 (en) * | 1986-03-19 | 1986-04-23 | Univ Strathclyde | Biochemical detector |
| GB8612861D0 (en) * | 1986-05-27 | 1986-07-02 | Cambridge Life Sciences | Immobilised enzyme biosensors |
| GB8710472D0 (en) * | 1987-05-01 | 1987-06-03 | Cambridge Life Sciences | Amperometric method |
-
1987
- 1987-05-01 GB GB878710472A patent/GB8710472D0/en active Pending
-
1988
- 1988-04-28 IE IE127488A patent/IE60238B1/en not_active IP Right Cessation
- 1988-04-28 IL IL86223A patent/IL86223A/xx not_active IP Right Cessation
- 1988-04-28 IL IL86222A patent/IL86222A0/xx unknown
- 1988-04-29 ES ES88303921T patent/ES2058271T3/es not_active Expired - Lifetime
- 1988-04-29 KR KR1019880701786A patent/KR960001501B1/ko not_active IP Right Cessation
- 1988-04-29 US US07/295,035 patent/US5122456A/en not_active Expired - Lifetime
- 1988-04-29 DE DE88303921T patent/DE3882078T2/de not_active Expired - Lifetime
- 1988-04-29 AU AU16862/88A patent/AU612366B2/en not_active Expired
- 1988-04-29 WO PCT/GB1988/000337 patent/WO1988008446A1/en unknown
- 1988-04-29 AU AU16861/88A patent/AU1686188A/en not_active Abandoned
- 1988-04-29 EP EP88303921A patent/EP0289344B1/en not_active Expired - Lifetime
- 1988-04-29 GB GB8810181A patent/GB2204698B/en not_active Expired - Lifetime
- 1988-04-29 JP JP63503840A patent/JP2528956B2/ja not_active Expired - Lifetime
- 1988-04-29 EP EP88303922A patent/EP0289345A1/en not_active Withdrawn
- 1988-04-29 JP JP63503839A patent/JPH01503409A/ja active Pending
- 1988-04-29 GB GB8810180A patent/GB2204415B/en not_active Expired - Lifetime
- 1988-04-29 WO PCT/GB1988/000338 patent/WO1988008447A1/en active IP Right Grant
- 1988-04-29 HU HU883273A patent/HU205625B/hu unknown
- 1988-05-02 MX MX011347A patent/MX167769B/es unknown
- 1988-05-02 CA CA000565696A patent/CA1304127C/en not_active Expired - Lifetime
- 1988-12-30 NO NO885836A patent/NO179953C/no not_active IP Right Cessation
- 1988-12-30 DK DK731988A patent/DK169559B1/da not_active IP Right Cessation
- 1988-12-30 FI FI886050A patent/FI92221C/sv not_active IP Right Cessation
- 1988-12-30 RU SU884613363A patent/RU1806187C/ru active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB | Publication of examined application | ||
| FG | Patent granted |
Owner name: CAMBRIDGE LIFE SCIENCES PLC |
|
| MA | Patent expired |